Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 29, Issue 4, Pages 1293-1301Publisher
ELSEVIER
DOI: 10.1016/j.msec.2008.10.026
Keywords
Shear assay technique; Cell/surface interfacial strengths; Computational fluid dynamics; Biomedical systems; BioMEMS surfaces
Categories
Funding
- Division of Material Research of the National Science Foundation [0231418]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [0231418] Funding Source: National Science Foundation
Ask authors/readers for more resources
The paper examines the adhesion Of human osterosarcoma (HOS) cells to selected biomaterials surfaces that are relevant to implantable biomedical systems and bio-micro-electro-mechanical systems (BioMEMS). The four biomaterials that were explored include: silicon. silicon coated with a nanoscale layer of titanium, Ti-6Al-4V, and poly-di-methyl-siloxane (PDMS). The interfacial strengths between the HOS cells and the biomaterials surfaces were determined using a shear assay technique. The adhesion forces were determined using a combination of confocal microscopy images of the three-dimensional cell structure, and computational fluid dynamics (CFD) Simulations that Coupled actual cell morphologies and non-Newtonian fluid properties in the computation of the adhesion forces. After cell detachment by the shear assay, immunofluorescence staining of the biomedical Surfaces was used to reveal the proteins associated with cell detachment. These revealed that the nano-scale Ti coating increases the cell/surface adhesion strength. Silicon with Ti coating has the strongest adhesion strength. while the other surfaces had similar adhesion strength. The measured strengths are shown to be largely associated with the detachment of focal adhesion proteins from extra-cellular matrix (ECM) proteins. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available