☆ 4.6 Article

Zinc protects sperm from being damaged by reactive oxygen species in assisted reproduction techniques

REPRODUCTIVE BIOMEDICINE ONLINE (2015)

Journal

REPRODUCTIVE BIOMEDICINE ONLINE

Volume 30, Issue 4, Pages 334-339

Publisher

ELSEVIER SCI LTD

DOI: 10.1016/j.rbmo.2014.12.008

Keywords

assisted reproduction; DNA damage; oxidative stress; reactive oxygen species; sperm

Categories

Obstetrics & Gynecology Reproductive Biology

Funding

Ethical Committee of the Faculty of The Second Affiliated Hospital of Fujian Medical University, China [20110311]
Youth Research Foundation of Fujian Provincial Department of Health, China [2011-1-36]

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Abstract

The aim of this study was to explore the effect of zinc on hydrogen peroxide-induced sperm damage in assisted reproduction techniques. First, sperms were selected from semen samples of 20 healthy men prepared by density gradient centrifugation. Selected sperm were treated with either 0.001% H2O2, 12.5 nM ZnCL2, 0.001% H2O2 + 12.5 nM ZnCL2 or 0.9% NaCl2 (control). After this treatment, the motility, viability, membrane integrity and DNA fragmentation of sperms in each group were analysed by Goodline sperm detection system, optical microscopy and sperm DNA fragmentation assay. Poorer motility, vitality, membrane integrity and more DNA damage were found in sperms treated by H2O2, compared with control. When sperms were treated with both H2O2 and zinc, however, all indicators were improved compared with H2O2 alone. There was a close association between oxidative stimulation and sperm injury; zinc could inhibit hydrogen peroxide-induced damage of sperm in assisted reproductive technology. However, the presence of zinc in culture medium can decrease the sperm quality without addition of peroxide. (C) 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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