Article
Biotechnology & Applied Microbiology
Kai Yu, Kai Xiao, Qin-qiang Sun, Run-feng Liu, Liang-feng Huang, Peng-fei Zhang, Hui-yan Xu, Yang-qing Lu, Qiang Fu
Summary: This study compared the protein correlation between spermatozoa, seminal plasma, and seminal plasma exosomes (SPEs) in buffalo and analyzed the protein differences between high-motility and low-motility SPEs. The protein composition of SPEs was mostly derived from spermatozoa and seminal plasma, and a functional network of reproductive process-related proteins was identified. The study also found differential protein expression between high-motility and low-motility SPEs. These findings provide valuable information for identifying biomarkers associated with male infertility.
Article
Agriculture, Dairy & Animal Science
Runfeng Liu, Xingchen Huang, Qinqiang Sun, Zhen Hou, Weihan Yang, Junjun Zhang, Pengfei Zhang, Liangfeng Huang, Yangqing Lu, Qiang Fu
Summary: Proteomic analysis of buffalo sperm reveals molecular mechanisms associated with poor motility, including inefficient energy metabolism, decreased sperm protamine proteins, a lack of motility-related proteins, and changes in tail structural proteins. These findings provide clues for the identification of molecular markers for poor motility in buffalo sperm.
Article
Veterinary Sciences
Wangsheng Zhao, Siraj Ahmed, Junxia Liu, Saeed Ahmed, Eugene Quansah, Tajmal Hussain Solangi, Yitao Wu, Yueling Yangliu, Hongmei Wang, Jiangjiang Zhu, Xin Cai
Summary: This study utilized iTRAQ proteomics to analyze differentially abundant proteins in yak and cattleyak epididymis, revealing 151 up-regulated and 137 down-regulated proteins in cattleyak compared to yak. Gene Ontology analysis showed that down-regulated proteins in cattleyak were primarily enriched in protein component acetylation, as well as negative and positive regulatory activities.
BMC VETERINARY RESEARCH
(2021)
Article
Cell Biology
Yongjie Xu, Qiu Han, Chaofeng Ma, Yaling Wang, Pengpeng Zhang, Cencen Li, Xiaofang Cheng, Haixia Xu
Summary: Comprehensive proteomics analysis of sperm from Yorkshire and Duroc boars revealed differential expression of proteins and phosphorylated proteins related to crucial physiological functions such as sperm development, motility, and energy metabolism. Proteins involved in axonemal dynein complex assembly and sperm-egg recognition were found to be significantly higher in Duroc spermatozoa, indicating potential differences in sperm function between the two boar breeds.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xi Luo, Mingming Liang, Shihai Huang, Qingsong Xue, Xuan Ren, Yanfang Li, Jinli Wang, Deshun Shi, Xiangping Li
Summary: Using iTRAQ-based proteomics analysis, this study discovered that the freezability of buffalo sperm was negatively associated with oxidative phosphorylation (OXPHOS), and found that peroxiredoxin 6 (PRDX6) had a protective effect on cryo-damage of frozen-thawed sperm.
Article
Obstetrics & Gynecology
Franz S. Gruber, Zoe C. Johnston, Neil R. Norcross, Irene Georgiou, Caroline Wilson, Kevin D. Read, Ian H. Gilbert, Jason R. Swedlow, Sarah Martins da Silva, Christopher L. R. Barratt
Summary: This study utilized a high-throughput screening platform to identify compounds that can enhance sperm motility, providing a promising starting point for male fertility drug discovery.
HUMAN REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Xinyue Hu, Lipeng Zhu, Qingyuan Ouyang, Junqi Wang, Jiwei Hu, Bo Hu, Shenqiang Hu, Hua He, Liang Li, Hehe Liu, Jiwen Wang
Summary: This study aimed to compare the semen quality of drakes with different levels of libido and identify the mechanisms regulating sperm motility by performing RNA-seq in the testis, epididymis, and spermaduct. The results showed that drakes with libido level 5 had significantly better sperm motility, testis weight, and epididymis organ index compared to drakes with libido level 4. Additionally, pathways related to metabolism, oxidative phosphorylation, immunity, proliferation, and signaling were significantly enriched in the testis, epididymis, and spermaduct. Several genes involved in protein digestion and absorption, cell cycle, Huntington disease, and PI3K-Akt signaling pathways were identified in the respective tissues. These findings provide new insights into the molecular mechanisms regulating sperm motility in drakes with different levels of libido.
Article
Agriculture, Dairy & Animal Science
Zhenwei Du, WenTing Li, Cong Liu, ChunXiu Wang, DongXue Wang, ShuaiPeng Zhu, XiangTao Kang, RuiRui Jiang, LeKun Deng, DongHua Li, GuiRong Sun
Summary: This study analyzed the testes of Gushi chicken roosters with high and low sperm motility using RNA-seq and identified differentially expressed RNAs involved in sperm motility. The study also revealed the pathways and genes related to sperm motility in chickens.
Article
Multidisciplinary Sciences
Mahmoud A. E. Hassan, Wael A. Khalil, Sameh A. Abdelnour, Reham Mokhtar Aman
Summary: This study found that adding ALAN during cryopreservation of buffalo sperm can significantly improve sperm motility, vitality, and membrane integrity. The ALAN150 group showed the best results and increased antioxidant capacity, glutathione reductase, and catalase activity, while decreasing lipid peroxidation and early apoptotic spermatozoa.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Xiaofan Tan, Yu He, Yanchun Qin, Zhiwei Yan, Jing Chen, Ruixue Zhao, Shenglan Zhou, David M. Irwin, Bojiang Li, Shuyi Zhang
Summary: Intramuscular fat (IMF) is an important regulator that affects meat quality. This study used quantitative proteomic analysis to identify candidate proteins associated with IMF in donkey muscle. Differentially abundant proteins analysis revealed proteins related to lipid metabolism and adipogenesis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Genetics & Heredity
Yingping Wu, Haiying Li, Xiaoyu Zhao, Gulnar Baki, Chen Ma, Yingying Yao, Jiahui Li, Yang Yao, Lin Wang
Summary: This study explored the potential biological function of circRNAs in sperm motility traits of Xinjiang Yili geese. The results suggest that circRNAs may interact with miRNAs to regulate mRNA and influence sperm motility, providing a reference for further analysis of the molecular mechanism of sperm motility regulation in Yili geese.
FRONTIERS IN GENETICS
(2022)
Article
Biotechnology & Applied Microbiology
Zhaozheng Yin, Xiuli Xu, Yuge Tan, Haiyue Cao, Wei Zhou, Xinyang Dong, Haiguang Mao
Summary: RNA sequencing of pigeon testicles identified multiple miRNAs associated with sperm motility, with mmu-miR-183-5p/FOXO1 and PC-3p-244994_31/CHDH pairs potentially affecting sperm motility.GO and KEGG analysis revealed target genes of differentially expressed miRNAs were related to kinase activity and signaling pathways.
Article
Reproductive Biology
Renzheng Zhang, Yiwei Chen, Pengjia Bao, Fude Wu, Chunnian Liang, Xian Guo, Min Chu, Ping Yan
Summary: This study identified proteins related to freezing tolerance in yak spermatozoa through proteomic analysis. The results showed that proteins involved in metabolic processes were upregulated in high-motility spermatozoa after frozen-thawed. Many antioxidant enzymes were also found to be upregulated in high-motility spermatozoa. Furthermore, KEGG analysis revealed the enrichment of differentially expressed proteins in glycolysis/gluconeogenesis, PPAR signaling pathway, and Ras signaling pathway. The findings of this study elucidate the mechanisms through which cryopreservation affects the movement of yak spermatozoa and offer a novel basis for refining freezing techniques and modifying cryopreservation extender components.
Article
Agriculture, Dairy & Animal Science
Chiara Del Prete, Olga Blanco Prieto, Beatrice Mislei, Eleonora Iacono, Gaetano Mari, Natascia Cocchia, Bianca Gasparrini, Barbara Merlo, Diego Bucci
Summary: The aim of this study was to verify the reliability of an open access CASA software (BGM) to evaluate the sperm motility of cattle and buffalo, comparing it to a commercial software (HTM). The results showed that the two systems' assessment of motility is comparable, but there are differences in some parameters due to algorithm variations. The open-access CASA plug-in is a reliable alternative to the expensive commercial CASA system.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Environmental Sciences
Xinzong Zhang, Zhaoyu Fan, Qiling Wang, Xinyi Deng, Ruijun Xu, Yingxin Li, Tingting Liu, Rui Wang, Chunxiang Shi, Suli Huang, Ziquan Lv, Gongbo Chen, Yong-Gang Duan, Yuewei Liu
Summary: This study reveals an association between ambient temperature and semen quality in South China, with higher temperatures leading to a reduction in semen quality. The findings indicate that a 5-degree Celsius increase in temperature during the 0-90 day period before ejaculation is associated with decreased sperm concentration, total sperm number, total motility, and normal sperm morphology. Furthermore, specific critical exposure windows of 10-14 days and 70-90 days before ejaculation were identified for sperm motility, count, and morphology.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Reproductive Biology
Zhen Hou, Qiang Fu, Yulin Huang, Pengfei Zhang, Fumei Chen, Mingxing Li, Zhuangzhuang Xu, Shun Yao, Dongrong Chen, Ming Zhang
Article
Biotechnology & Applied Microbiology
Yanling Zhao, Xiaoli Lu, Zhipeng Cheng, Mengfang Tian, Yangzong Qiangba Qiang, Qiang Fu, Zili Ren
Article
Reproductive Biology
Qiang Fu, Limei Pan, Delun Huang, Zhiqiang Wang, Zhen Hou, Ming Zhang
Article
Agriculture, Dairy & Animal Science
Fumei Chen, Qiang Fu, Liping Pu, Zhen Hou, Zhuangzhuang Xu, Pengfei Zhang, Tingxian Deng, Chunying Pang, Xianwei Liang, Yangqing Lu, Ming Zhang
REPRODUCTION IN DOMESTIC ANIMALS
(2020)
Article
Reproductive Biology
Pengfei Zhang, Wengtan He, Yulin Huang, Kai Xiao, Yuyan Tang, Liangfeng Huang, Xingchen Huang, Junjun Zhang, Weihan Yang, Runfeng Liu, Qiang Fu, Yangqing Lu, Ming Zhang
Summary: This study conducted an integrative analysis of the proteome and phosphoproteome of Sertoli cells in buffalo testes to explore their role in spermatogenesis. Multiple proteins were identified, with some being involved in gene expression, cellular localization, and transport processes. The results offer insights into male reproduction and provide a foundation for future research.
Article
Genetics & Heredity
Liangfeng Huang, Kai Xiao, Junjun Zhang, Pengfei Zhang, Wengtan He, Yuyan Tang, Weihan Yang, Xingchen Huang, Runfeng Liu, Xianwei Liang, Xingting Liu, Qiang Fu, Yangqing Lu, Ming Zhang
Summary: This study analyzed the transcriptome profiles of immature Leydig cells (ILC) and adult Leydig cells (ALC) in buffalo, identifying key genes and pathways associated with the regulation of testosterone secretion function. The findings suggest that cAMP signaling plays a crucial role in maintaining testosterone function during postnatal development of LCs, with ALCs potentially increasing testosterone levels through enhanced cAMP production compared to ILCs. Protein-protein interaction analysis highlighted hub genes in cAMP signaling pathway, providing insights into molecular mechanisms of LCs regulating spermatogenesis.
Article
Agriculture, Dairy & Animal Science
Xingchen Huang, Qinqiang Sun, Dongrong Chen, Weihan Yang, Junjun Zhang, Runfeng Liu, Pengfei Zhang, Liangfeng Huang, Ming Zhang, Qiang Fu
Summary: This study aimed to investigate the expression of Nlrp5 and Tle6 genes in buffalo. The coding sequences of these two genes were successfully cloned and characterized. Results showed an absence of Nlrp5 or Tle6 transcripts in somatic tissues, except in the ovary. The expression levels of Nlrp5 and Tle6 in oocytes increased during early embryonic development and then decreased. Protein expression patterns were confirmed by immunofluorescence analysis.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Genetics & Heredity
Junjun Zhang, Liangfeng Huang, Pengfei Zhang, Xingchen Huang, Weihan Yang, Runfeng Liu, Qinqiang Sun, Yangqing Lu, Ming Zhang, Qiang Fu
Summary: This study conducted an identification, evolution, and expression analysis of the BRD gene family in the swamp buffalo. The BRD proteins were classified into six groups, and the BRD genes were irregularly distributed in 13 chromosomes. Comparative transcriptomics revealed differential expression of BRD genes in Sertoli cells at different developmental stages of buffalo.
Article
Agriculture, Dairy & Animal Science
Weihan Yang, Runfeng Liu, Qinqiang Sun, Xingchen Huang, Junjun Zhang, Liangfeng Huang, Pengfei Zhang, Ming Zhang, Qiang Fu
Summary: This study found that quercetin could attenuate the effects of endoplasmic reticulum stress on granulosa cells by the PERK/CHOP signaling pathway, providing a novel strategy for inhibiting the apoptosis of granulosa cells.
Article
Agriculture, Dairy & Animal Science
Runfeng Liu, Xingchen Huang, Qinqiang Sun, Zhen Hou, Weihan Yang, Junjun Zhang, Pengfei Zhang, Liangfeng Huang, Yangqing Lu, Qiang Fu
Summary: Proteomic analysis of buffalo sperm reveals molecular mechanisms associated with poor motility, including inefficient energy metabolism, decreased sperm protamine proteins, a lack of motility-related proteins, and changes in tail structural proteins. These findings provide clues for the identification of molecular markers for poor motility in buffalo sperm.
Article
Multidisciplinary Sciences
Liangfeng Huang, Junjun Zhang, Pengfei Zhang, Xingchen Huang, Weihan Yang, Runfeng Liu, Qinqiang Sun, Yangqing Lu, Ming Zhang, Qiang Fu
Summary: This study analyzed over 32,000 cells from buffalo testes using single-cell RNA sequencing, generating dynamic gene expression roadmaps of germ and somatic cell development. It uncovered the global cell-cell communication essential for regular spermatogenesis in the buffalo testicular microenvironment. These findings provide the theoretical basis for establishing buffalo germline stem cells in vitro or culturing organoids, as well as facilitating the expansion of superior livestock breeding.
Article
Biochemistry & Molecular Biology
Qiang Fu, Yulin Huang, Zhiqiang Wang, Fumei Chen, Delun Huang, Yangqing Lu, Xianwei Liang, Ming Zhang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2016)
Article
Biochemistry & Molecular Biology
Qiang Fu, Zhen-Fang Liu, Yu-Lin Huang, Yang-Qing Lu, Ming Zhang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2016)
Article
Reproductive Biology
Q. Fu, M. Zhang, W. S. Qin, Y. Q. Lu, H. Y. Zheng, B. Meng, S. S. Lu, K. H. Lu
