Article
Biology
Louise C. Archer, Stephen A. Hutton, Luke Harman, W. Russell Poole, Patrick Gargan, Philip McGinnity, Thomas E. Reed
Summary: This study reveals complex associations between metabolic rate and growth rate in brown trout under different thermal environments, indicating a thermally sensitive metabolic phenotype with adaptive responses. The findings suggest that fluctuating selection related to growth plays an important role in shaping metabolic variation and adapting to climate change.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Microbiology
Katharina Keiz, Sebastian Ulrich, Jasmin Wenderlein, Patrick Keferloher, Anna Wiesinger, Klaus Neuhaus, Ilias Lagkouvardos, Helmut Wedekind, Reinhard K. Straubinger
Summary: The bacterial community of brown trout from unfertilized egg to 95 days post fertilization (dpf) was studied using 16S rRNA gene amplicon sequencing. Significant changes in bacterial richness, microbial diversity, and taxonomic composition were observed during early developmental stages until 67 dpf, while the bacterial community seemed to stabilize in the fry stages from 67 to 95 dpf.
Article
Fisheries
Gasper Renko, Ida Djurdjevic, Simona Susnik Bajec
Summary: A novel protocol for the isolation of chromatophores from brown trout skin was developed based on previous research on zebrafish, enabling specific gene expression evaluation. The method is simple, fast, and ethical, allowing for monitoring at multiple time points, and should be effective for the majority of salmonid species due to similarities in skin morphology.
Article
Environmental Sciences
Dhamotharan Kannimuthu, HyeongJin Roh, H. Craig Morton, Ma Michelle D. Penaranda, Anne Vossgard, Tom Hansen, Per Gunnar Fjelldal, Egil Karlsbakk, Ingrid Fiksdal, Maria K. Dahle, Hakon Berg-Rolness, Stig Maehle, Ghebretnsae Dawit Berhe, Joachim Nordbo, Sonal Patel, Abdullah Madhun, Soren Grove, Bjorn Olav Kvamme
Summary: PRV-1 can infect all developmental stages of Atlantic salmon and brown trout, but brown trout have a lower infection prevalence. All life stages of Atlantic salmon develop heart lesions characteristic of HSMI, while brown trout only exhibit mild infections without pathological changes in target organs. These results strongly suggest that brown trout are less susceptible to PRV-1 infection compared to Atlantic salmon, confirming the species-specific susceptibility and disease development for PRV-1 infection.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Food Science & Technology
Muhammed Atamanalp, Arzu Ucar, Esat Mahmut Kocaman, Gonca Alak
Summary: The research aimed to determine the shelf life of products obtained by using humic acid in trout culture, finding that while humic acid did not affect the chemical composition, color, and texture, it had a significant impact on mineral substance, pH, TBARS, TVB-N, and microbiological results. During storage, there were fluctuations in all analyzed values, but the group with 2% humic acid showed relatively lower values in microbiological and physico-chemical analysis results at the end of the storage period.
FOOD PACKAGING AND SHELF LIFE
(2021)
Article
Fisheries
Robert Kasprzak, Zdzislaw Zakes, Maciej Kamaszewski, Hubert Szudrowicz, Wiktoria Wiechetek, Julia Renata Janusz, Teresa Ostaszewska, Agata Korzelecka-Orkisz, Krzysztof Formicki
Summary: The conservation of brown trout populations is becoming increasingly important due to environmental degradation. This study assessed the effects of dietary immunostimulants on two morphs of brown trout and found that feeding with a diet enriched with immunostimulants for 2 weeks may enhance rearing protocols for the fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Giusy Rusco, Michele Di Iorio, Stefano Esposito, Pierpaolo Gibertoni, Emanuele Antenucci, Valentino Palombo, Alessandra Roncarati, Nicolaia Iaffaldano
Summary: In external fertilizer fish, ovarian fluid (OF) plays a key role in improving sperm swimming performance and reducing the risk of genetic introgression of wild populations from introduced individuals.
Article
Fisheries
Kristi Kaello, Henrik Baktoft, Martin Lykke Kristensen, Kim Birnie-Gauvin, Kim Aarestrup
Summary: Natal homing is a common strategy among salmonids, but not all individuals return to their natal river and may stray. This study investigated straying in an anadromous brown trout population and found that 36% of individuals were detected in non-natal rivers. Individuals spending longer time at sea were less likely to stray. The likelihood of straying also depended on the developmental status during out-migration, with lower likelihood for parr compared to pre-smolt and smolt.
ICES JOURNAL OF MARINE SCIENCE
(2022)
Article
Fisheries
Zoran Marinovic, Jelena Lujic, Simona Susnik Bajec, Ida Djurdjevic, Ales Snoj, Gyorgy Hoitsy, Bela Urbanyi, Akos Horvath
Summary: The study found that rainbow trout is not a suitable recipient for all salmonid species, highlighting the importance of careful recipient selection in the development of surrogate production technology.
AQUACULTURE REPORTS
(2022)
Article
Ecology
Richard D. Durtsche, Bror Jonsson, Larry A. Greenberg
Summary: Elevated egg incubation temperature leads to lower metabolic rates in juvenile brown trout, potentially impacting their energy expenditure and predatory abilities in a future warmer climate. The results support the countergradient variation hypothesis, showing that thermal conditions can produce phenotypic patterns in metabolic rates. The differences observed were likely due to plastic responses rather than genetic differences among populations.
Article
Agriculture, Dairy & Animal Science
Kamil Uney, Ertugrul Terzi, Duygu Durna Corum, Rahmi Can Ozdemir, Soner Bilen, Orhan Corum
Summary: The pharmacokinetic/pharmacodynamic studies revealed non-linear and dose-disproportional pharmacokinetics of enrofloxacin in brown trout. Single oral administration of 10 and 20 mg/kg of enrofloxacin provided long duration of action, potentially reducing handling and stress in brown trout. Further research is needed for successful treatment of infections caused by Aeromonas hydrophila and A. sobria in brown trout.
Article
Biology
Ouellet Valerie, Melinda D. Daniels
Summary: The study examined the shift in thermal habitat occupation for Brook and Brown Trout populations during the summer, with an increase in Brown Trout and a decrease in Brook Trout numbers. The observed population shifts were driven by optimal growth temperatures rather than elevated water temperatures, potentially affecting interactions and survival throughout the summer.
JOURNAL OF THERMAL BIOLOGY
(2021)
Article
Marine & Freshwater Biology
Tormod Haraldstad, Erik Hoglund, Frode Kroglund, Esben M. Olsen, Kate L. Hawley, Thrond O. Haugen
Summary: Hydropower poses a serious threat to anadromous salmonids by hindering migration and causing turbine mortality. This study evaluates the long-term selection mechanisms affecting fish populations caused by turbines. The survival rates of wild brown trout smolts that passed through turbines and those that bypassed them were compared, revealing lower survival rates and smaller size in turbine migrants. The resulting disruptive selection may lead to increased size variation in smolt phenotypes. Mitigation measures at hydropower plants are crucial for maintaining sustainable populations and genetic variation.
Article
Fisheries
Jan Grimsrud Davidsen, Andrine Emilie Halvorsen, Sindre Havarstein Eldoy, Eva Bonsak Thorstad, Leif Asbjorn Vollestad
Summary: Brown trout and Arctic charr migrated from fresh water to the sea mainly in May and June, with large individual variation in migration timing. Large brown trout migrated earlier than small ones, while no such pattern was found for Arctic charr. Brown trout stayed longer at sea than Arctic charr, and the two species showed differences in their choice of marine areas and feeding strategies. Stable isotope analysis revealed that brown trout fed on marine fish and shrimps, while Arctic charr had a diet with a larger proportion of freshwater invertebrates.
JOURNAL OF FISH BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Konrad Ocalewicz
Summary: The development of haploid embryos with only paternal or maternal chromosomes requires irradiation or activation of eggs with irradiated spermatozoa. To obtain doubled haploids, androgenetic and gynogenetic haploid zygotes need to undergo thermal or high hydrostatic pressure shock. The use of doubled haploids has been important in breeding programs and studying genetic phenotypes. However, the reduced survival rate of doubled haploids limits their application. Recent studies have provided insights into the characteristics of fish eggs with high and low competence for androgenesis and mito-gynogenesis.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Fisheries
Maciej Wozny, Stefan Dobosz, Piotr Hliwa, Piotr Gomulka, Jaroslaw Krol, Kazimierz Obremski, Jana Blahova, Zdenka Svobodova, Oliwia Michalik, Konrad Ocalewicz, Pawel Brzuzan
Article
Fisheries
J. Nynca, S. Judycka, E. Liszewska, S. Dobosz, M. Krzys, A. Ciereszko
Summary: Freezing-thawing cycles have been found to preserve sperm motility in different fish species, but with a decrease in motility. Fertilizing ability of rainbow trout sperm did not differ after one freeze-thaw cycle, but significant decreases were recorded after two cycles. Double cryopreservation of fish semen shows potential benefits for preserving endangered fish species and valuable breeding aquaculture individuals.
Article
Genetics & Heredity
Ligia Panasiak, Stefan Dobosz, Konrad Ocalewicz
Article
Genetics & Heredity
Dominik Matoulek, Veronika Boruvkova, Konrad Ocalewicz, Radka Symonova
Summary: The study of fish cytogenetics has been hindered by the challenge of producing G-bands. However, a new Python tool has been developed to visualize and quantify GC% and repeats along fish chromosomes, showing the potential contribution of repeats to the inability to produce G-banding.
Article
Biology
Qiaowei Pan, Romain Feron, Elodie Jouanno, Hugo Darras, Amaury Herpin, Ben Koop, Eric Rondeau, Frederick W. Goetz, Wesley A. Larson, Louis Bernatchez, Mike Tringali, Stephen S. Curran, Eric Saillant, Gael P. J. Denys, Frank A. von Hippel, Songlin Chen, J. Andres Lopez, Hugo Verreycken, Konrad Ocalewicz, Rene Guyomard, Camille Eche, Jerome Lluch, Celine Roques, Hongxia Hu, Roger Tabor, Patrick DeHaan, Krista M. Nichols, Laurent Journot, Hugues Parrinello, Christophe Klopp, Elena A. Interesova, Vladimir Trifonov, Manfred Schartl, John Postlethwait, Yann Guiguen
Summary: This study traced the evolution of sex determination in an entire teleost order through following the fate of a master sex-determining gene in northern pike, revealing that the gene originated from a gene duplication event 65 to 90 million years ago and remained sex linked on undifferentiated sex chromosomes for at least 56 million years in multiple species. Various independent species- or population-specific sex determination transitions, including the recent loss of a Y chromosome, were identified. Occasional sex reversals and genetic bottlenecks were proposed as non-adaptive explanations for sex determination transitions.
Article
Agriculture, Dairy & Animal Science
Piotr Hliwa, Ligia Panasiak, Elzbieta Ziomek, Rafal Rozynski, Lukasz Leonowicz, Joanna Grudniewska, Stefan Dobosz, Konrad Ocalewicz
Summary: The use of parental individuals from allochthonous lineages for stocking specimens of European grayling has led to genetic contamination in local populations. This study aimed to triploidize the grayling and examine the gonads of triploid individuals. The results showed that triploid grayling exhibited sterile gonads, indicating their inability to reproduce.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ligia Panasiak, Karolina Szubert, Marcin Polonis, Konrad Ocalewicz
Summary: The study found that rainbow trout with normal growth and dwarfism have similar telomere lengths, indicating that morphological and physiological differences do not affect the dynamics of telomeric DNA in fish with different growth rates. Despite significantly lower body length and weight in dwarf fish, there was no correlation found between body size and telomere length. The presence of active telomerase in rainbow trout suggests that any variations in telomere length may be leveled out.
JOURNAL OF APPLIED GENETICS
(2022)
Article
Developmental Biology
Ligia Panasiak, Marcin Kucinski, Agata Blaszczyk, Konrad Ocalewicz
Summary: This study examined the telomerase activity in specimens with retarded growth and their normally developed siblings in rainbow trout. The results showed that dwarf specimens did not exhibit significant differences in liver and muscle telomerase activity compared to the rainbow trout without growth deficiency, while telomerase activity in the skin was significantly upregulated in the normally developed specimens.
Review
Biochemistry & Molecular Biology
Konrad Ocalewicz
Summary: The development of haploid embryos with only paternal or maternal chromosomes requires irradiation or activation of eggs with irradiated spermatozoa. To obtain doubled haploids, androgenetic and gynogenetic haploid zygotes need to undergo thermal or high hydrostatic pressure shock. The use of doubled haploids has been important in breeding programs and studying genetic phenotypes. However, the reduced survival rate of doubled haploids limits their application. Recent studies have provided insights into the characteristics of fish eggs with high and low competence for androgenesis and mito-gynogenesis.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Marcin Kucinski, Magdalena Jakubowska-Lehrmann, Agnieszka Gora, Zuzanna Mirny, Katarzyna Nadolna-Altyn, Joanna Szlinder-Richert, Konrad Ocalewicz
Summary: The aim of this study was to investigate the genetic diversity and population structure of the European flounder and determine if the Baltic flounder is present in the southern Baltic Sea. Additionally, the study aimed to determine if the decline in body condition indices of the species in the Baltic Sea is due to adaptive changes in its gene pool caused by increased fishing pressure. The results showed that the European flounder had a high level of genetic diversity and represented a single genetic cluster. No presence of the Baltic flounder was detected, and there were no signs of directional selection or density-dependent adaptive changes in the gene pool.
Article
Marine & Freshwater Biology
Krzysztof Jagiello, Marcin Polonis, Konrad Ocalewicz
Summary: Comparing the body abnormalities in diploid and triploid 14-month-old rainbow trout from commercial stocks, this research found a higher proportion of externally observed body deformities in triploid specimens, including spine deformities such as compressions and fusions of vertebrae. These abnormalities, though non-lethal, may negatively impact the condition and aesthetics of the fish, leading to potential economic losses in commercial production.
OCEANOLOGICAL AND HYDROBIOLOGICAL STUDIES
(2021)
Article
Marine & Freshwater Biology
Marcin Polonis, Agata Blaszczyk, Krzysztof Jagiello, Ligia Panasiak, Stefan Dobosz, Konrad Ocalewicz
Summary: Ionizing radiation was used to inactivate the nuclear genome in rainbow trout eggs for induced androgenetic development. However, the study found significant inter-clutch differences in the developmental competence of eggs from different females, suggesting a strong maternal effect on androgenesis efficiency.
OCEANOLOGICAL AND HYDROBIOLOGICAL STUDIES
(2021)
Article
Agriculture, Dairy & Animal Science
Krzysztof Jagiello, Marcin Polonis, Ligia Panasiak, Stefan Dobosz, Konrad Ocalewicz
Summary: In this study, triploid, mitogynogenetic (doubled haploid; DH) and meiogynogenetic brook trout were produced to assess the efficiency of these technologies and the potential susceptibility of chromosome set manipulated individuals for spinal disorders. The highest survival rates were observed among triploid brook trout, while DHs showed the highest mortality. Additionally, significantly increased malformation rates were observed in meiogynogenetic and mitogynogenetic individuals compared to non-manipulated diploids. The study discusses genetic factors and side effects of HHP shock that may impact survival rates and prevalence of skeletal deformities in chromosome set manipulated brook trout.
ANNALS OF ANIMAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Konrad Pomianowski, Konrad Ocalewicz
Summary: Hybrids of Arctic char and brook trout show viable and fertile offspring, impacting the gene pool of native Arctic char. The study reveals chromosome fragments, presence of triploid individuals, and inherited telomeric sequences in the hybrid progeny. Additionally, chromosomal rearrangements were observed in hybrids, influencing the genome organization of Salvelinus hybrids.
JOURNAL OF APPLIED GENETICS
(2021)