Journal
MASS SPECTROMETRY REVIEWS
Volume 30, Issue 3, Pages 417-434Publisher
WILEY
DOI: 10.1002/mas.20287
Keywords
MALDI-MS; bacteria profiling; microorganism identification
Categories
Funding
- Ministry of Education, Youth and Sports of the Czech Republic [LC06034, MSM0021622415, MSM0021622416]
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Direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed. (C) 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:417-434, 2011
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