Journal
MALARIA JOURNAL
Volume 11, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1475-2875-11-198
Keywords
Cambodia; malaria; Plasmodium falciparum; HRP-2; Anti-malarial drugs; Drug resistance
Categories
Funding
- AFRIMS
- Cambodian clinical and laboratory
- US Department of Defense Global Emerging Infections System
- Bill & Melinda Gates Foundation World Health Organization (via World Health Organization)
- Medicines for Malaria Venture (MMV)
- US Army Military Infectious Disease Research Program (MIDRP)
- US Army Medical Materiel Development Activity (USAMMDA)
- Fort Detrick, Maryland, USA
- Silver Spring, Maryland, USA
Ask authors/readers for more resources
Background: In vitro drug susceptibility assay of Plasmodium falciparum field isolates processed immediate ex vivo (IEV), without culture adaption, and tested using histidine-rich protein-2 (HRP-2) detection as an assay, is an expedient way to track drug resistance. Methods: From 2005 to 2010, a HRP-2 in vitro assay assessed 451 P. falciparum field isolates obtained from subjects with malaria in western and northern Cambodia, and eastern Thailand, processed IEV, for 50% inhibitory concentrations (IC50) against seven anti-malarial drugs, including artesunate (AS), dihydroartemisinin (DHA), and piperaquine. Results: In western Cambodia, from 2006 to 2010, geometric mean (GM) IC50 values for chloroquine, mefloquine, quinine, AS, DHA, and lumefantrine increased. In northern Cambodia, from 2009-2010, GM IC50 values for most drugs approximated the highest western Cambodia GM IC50 values in 2009 or 2010. Conclusions: Western Cambodia is associated with sustained reductions in anti-malarial drug susceptibility, including the artemisinins, with possible emergence, or spread, to northern Cambodia. This potential public health crisis supports continued in vitro drug IC50 monitoring of P. falciparum isolates at key locations in the region.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available