Article
Chemistry, Analytical
Alan Wong
Summary: High-Resolution Magic-Angle Spinning (HRMAS) NMR spectroscopy is considered an essential technique in metabolomics for assessing the biochemical composition of tissue samples. HRMAS localized profiling shows promise for spatial resolution of metabolic profiles within the sampling tissues.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Physical
Ivan Hung, Eric G. Keeler, Wenping Mao, Peter L. Gor'kov, Robert G. Griffin, Zhehong Gan
Summary: This study addresses the challenges of low sensitivity, low resolution, and large quadrupolar couplings in the NMR study of oxygen by employing efficient isotopic labeling, high magnetic fields, fast sample spinning, and H-1 detection. Oxygen sites specific to each amino acid residue are observed through multidimensional experiments. The use of cross-polarization at high sample spinning frequencies enables efficient C-13<->O-17 polarization transfer. Results show that using O-17 for initial polarization provides better sensitivity per unit time compared to H-1.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Karolina Krajewska, Anna M. Golkowska, Maciej Nowak, Marta Kozakiewicz-Latala, Wojciech Pudlo, Andrzej Zak, Bozena Karolewicz, Yaroslav Z. Khimyak, Karol P. Nartowski
Summary: This study proposes the use of HR-MAS NMR spectroscopy for a molecular level characterisation of the external surface of carbohydrate-functionalised nanoparticles. The results demonstrate that HR-MAS NMR can provide detailed structural information on the organic functionalities attached at the external surface of MSN within short experimental times.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physics, Multidisciplinary
Alexander Prehn, Martin Ibruegger, Gerhard Rempe, Martin Zeppenfeld
Summary: The rapid progress in cooling and trapping of molecules has enabled high-resolution spectroscopy of trapped diatomic molecules for the first time, showing unprecedented precision. Extending this work to polyatomic molecules offers unique opportunities due to their more complex geometries. By combining various techniques, a high-resolution spectroscopy of formaldehyde molecules with reduced Stark broadening and improved precision has been achieved.
PHYSICAL REVIEW LETTERS
(2021)
Review
Biophysics
Leo L. Cheng
Summary: This article provides a history of the discovery and recent developments of high-resolution magic angle spinning (HRMAS) NMR, a technique used for intact biological material analysis. HRMAS NMR metabolomics studies have been applied in various fields such as human disease states, plant and food analysis, and cell structure studies. The article also discusses the potential applications of HRMAS NMR in future biomedical investigations.
NMR IN BIOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Roman Zadorozhnyi, Sucharita Sarkar, Caitlin M. Quinn, Kaneil K. Zadrozny, Barbie K. Ganser-Pornillos, Owen Pornillos, Angela M. Gronenborn, Tatyana Polenova
Summary: Histidine residues in proteins play crucial structural and functional roles, such as metal-binding and enzyme catalysis. This study introduces a fast MAS NMR approach to determine the protonation and tautomeric states of His at frequencies of 40-62 kHz, using H-1 detection and TEDOR-based filters in 2D heteronuclear correlation experiments. The approach is demonstrated using microcrystalline assemblies of HIV-1 CA(CTD)-SP1 protein.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Radiology, Nuclear Medicine & Medical Imaging
Matteo Sanchez-Dahl Gonzalez, Isabella H. Muti, Leo L. Cheng
Summary: This review discusses the potential clinical benefits of incorporating high-resolution magic angle spinning magnetic resonance spectroscopy in the diagnosis, prognostication, and monitoring of prostate cancer.
MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Gary S. Howarth, Ann E. McDermott
Summary: This study investigates the structure of the cytosolic N- and C-termini of the pH-activated bacterial potassium channel KcsA. The researchers used HR-MAS and fractional deuteration as tools to study these poorly resolved regions. They found that the C-terminus transitions from a rigid structure to a more dynamic structure in acidic solution, and made previously unreported assignments of residues in the C-terminus of lipid-embedded channels.
Article
Biotechnology & Applied Microbiology
Danijela Stanisic, Guilherme C. F. Cruz, Leonardo Abdala Elias, Junko Tsukamoto, Clarice W. Arns, Douglas Soares da Silva, Stanislav Mochkalev, Raluca Savu, Ljubica Tasic
Summary: This study evaluated the antiviral and non-toxic effects of silver nanoparticles on in vitro cells infected with coronavirus using High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance (HR-MAS NMR) spectroscopy. The bio-inspired synthesis of silver nanoparticles using an orange flavonoid-hesperetin (HST) was successful, and the nanoparticles showed great colloidal stability and antiviral activity. The fabricated nanomaterials inhibited viral activity and showed no toxicity towards the cells. The findings suggest potential antiviral applications for the 3D flexible nanostructured composite and colloidal spray.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Computer Science, Information Systems
Pei Dong, Lei Wu, Lei Meng, Xiangxu Meng
Summary: This paper proposes a progressive adversarial learning algorithm, HR-PrGAN, for generating high-resolution image sequences with rich details. The algorithm includes coarse-grain and refinement stages, with loss functions and a feature supplementary module used to maintain image consistency and improve image details. Experimental results demonstrate that the proposed method outperforms state-of-the-art methods in image synthesis and quality evaluation based on human perception.
INFORMATION SCIENCES
(2022)
Article
Astronomy & Astrophysics
G. M. Wahlgren, K. E. Nielsen, D. S. Leckrone
Summary: The study reveals that the element distribution in HR 3383 is consistent with other hot Am stars, but differs from cooler HgMn stars in terms of enhancements in platinum-group elements. The current theory and calculations are able to predict observed abundances and trends up to the iron group.
MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY
(2021)
Article
Chemistry, Physical
DeAunna A. A. Daniels, Thresa A. A. Wells, Peter C. C. Chen
Summary: Two-dimensional infrared (2DIR) spectroscopy is an established method for studying vibrational spectra in condensed phase samples, but the spectral resolution is often insufficient for resolving rotational peaks in gas phase spectra. A new method for generating high resolution 2DIR spectra of gas phase molecules has been developed and tested using methane as the sample. The ability to generate easily recognizable 2D rovibrational patterns makes this technique useful for applications that are otherwise difficult or impossible with conventional 1D rovibrational spectroscopy.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Geosciences, Multidisciplinary
Sansar Raj Meena, Lorenzo Nava, Kushanav Bhuyan, Silvia Puliero, Lucas Pedrosa Soares, Helen Cristina Dias, Mario Floris, Filippo Catani
Summary: This study presents a high-resolution satellite dataset for landslide mapping and detection, which has been tested globally and shown to be reliable and robust in deep learning models.
EARTH SYSTEM SCIENCE DATA
(2023)
Article
Environmental Sciences
Dahang Wan, Rongsheng Lu, Sailei Wang, Siyuan Shen, Ting Xu, Xianli Lang
Summary: This paper proposes an enhanced YOLOv5 algorithm for object detection in high-resolution optical remote sensing images, which uses multiple layers of the feature pyramid, a multi-detection-head strategy, and a hybrid attention module to improve the effect of object-detection networks.
Review
Chemistry, Multidisciplinary
Bernd Reif
Summary: This article summarizes the importance of proton detection in studying solid-state biomolecules over the past 20 years, and introduces labeling strategies and spectroscopic methods for experiments using perdeuterated samples to obtain quantitative information on structure and dynamics.