Journal
MACROMOLECULAR RAPID COMMUNICATIONS
Volume 35, Issue 13, Pages 1191-1197Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201400120
Keywords
block copolymers; GPMA; HPMA; siRNA delivery; microscale thermophoresis
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Funding
- [SFB 1066 (A7, Q2)]
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Here, the preparation of a novel block copolymer consisting of a statistical copolymer N-(2-hydroxypropyl) methacrylamide-s-N-(3-aminopropyl) methacrylamide and a short terminal 3-guanidinopropyl methacrylamide block is reported. This polymer structure forms neutral but water-soluble nanosized complexes with siRNA. The siRNA block copolymer complexes are first analyzed using agarose gel electrophoresis and their size is determined with fluorescence correlation spectroscopy. The protective properties of the polymer against RNA degradation are investigated by treating the siRNA block copolymer complexes with RNase V1. Heparin competition assays confirm the efficient release of the cargo in vitro. In addition, the utilization of microscale thermophoresis is demonstrated for the determination of the binding strength between a fluorescently labeled polyanion and a polymer molecule.
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