Journal
MACROMOLECULAR RAPID COMMUNICATIONS
Volume 33, Issue 18, Pages 1510-1516Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201200441
Keywords
conjugated polymers; cysteine sensor; fluorescence; sensors; water-soluble hyperbranched polymers
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Funding
- National Research Foundation of Korea (NRF) [2012R1A2A2A01004979, 2012H1B8A2025978]
- Ministry of Education, Science and Technology
- National Research Foundation of Korea [2012H1B8A2025978, 핵06B1310, 2012R1A2A2A01004979] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A highly efficient colorimetric and fluorescence turn-off probe for the sensitive and selective detection of the biologically important amino acid, cysteine (Cys), is demonstrated using a newly synthesized water-soluble hyperbranched polymer (HP) containing sulfonic acid groups. The detection mechanism involves two steps: (i) the slight quenching of HP in the presence of Co2+ in advance; and (ii) the gradual quenching of the HPCo2+ complex according to the concentration of Cys due to the absorption screening effect of the formation of the Cys-Co2+ complex, which prevents HP from absorbing excitation energy.
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