Article
Food Science & Technology
Justyna Szulc, Artur Kolodziej, Tomasz Ruman
Summary: This study utilized innovative laser mass spectroscopy methods to detect and quantify mycotoxins on building materials, with (109)AgNPs SALDI MS showing the lowest detection limits and highest signal intensity. The (109)AgNPs method may serve as an alternative to commonly used MALDI MS and liquid chromatography tandem mass spectrometry LC-MS/MS for mycotoxin determination in the future.
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Medicine, General & Internal
Tjitske Zaat, Miriam Zagers, Femke Mol, Mariette Goddijn, Madelon van Wely, Sebastiaan Mastenbroek
Summary: Based on the study, the 'freeze all' strategy may not have a significant difference compared to the conventional IVF/ICSI strategy in terms of cumulative live birth rate and ongoing pregnancy rate, but may have a lower risk of ovarian hyperstimulation syndrome. However, other risks such as maternal hypertensive disorders of pregnancy and having a large-for-gestational-age baby may increase with the 'freeze all' strategy.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Article
Chemistry, Analytical
Nicole Strittmatter, Richard M. England, Alan M. Race, Daniel Sutton, Jennifer Moss, Gareth Maglennon, Stephanie Ling, Edmond Wong, Jonathan Rose, Ian Purvis, Ruth Macdonald, Simon T. Barry, Marianne B. Ashford, Richard J. A. Goodwin
Summary: A strategy was presented to perform imaging mass cytometry experiments on a water-soluble polysarcosine-modified dendrimer drug-delivery system. By conducting two consecutive imaging acquisitions on the same tissue section, subsequent antibody staining and visualization were achieved. This workflow allowed imaging at a lateral resolution of 2 microns.
ANALYTICAL CHEMISTRY
(2021)
Article
Automation & Control Systems
Adam J. Taylor, Dimitrios Tsikritsis, Alex Dexter, Amy Burton, Josephine Bunch, Natalie A. Belsey
Summary: Rapid chemical characterization methods can assess and classify differences in solid oral dosage forms, helping to identify off-brand or counterfeit products.
JOURNAL OF CHEMOMETRICS
(2022)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Schalk Cloete, Mohammed Nazeer Khan, Shareq Mohd Nazir, Shahriar Amini
Summary: The study proposes a method for ammonia production using membrane-assisted autothermal reforming to reduce CO2 emissions and increase efficiency, with significant economic advantages compared to traditional ammonia plants.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Chemical
Salar Jabbary Farrokhi, Hossein Pakzad, Mohammadali Fakhri, Ali Moosavi
Summary: This study investigates the separation capacities of sponges sprayed with a superhydrophobic coating for a broad range of oils and solvents. Results show that the least dense sponge has the highest absorption capacity, while the absorption capacity varies erratically with the dynamic viscosity. In addition, the coated sponges maintain their superhydrophobicity after being subjected to extreme conditions.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Yong-Min Kim, Sung-Woo Park, Mi-Jin Lee, Da-Yeon Jeon, Su-Jin Sa, Yong-Dae Jeong, Ha-Seung Seong, Jung -Woo Choi, Shinichi Hochi, Eun-Seok Cho, Hak-Jae Chung
Summary: In this study, the iTRAQ technique and LC-MS/MS analysis were used to identify differentially expressed CM10-fractionated proteins in fresh and frozen-thawed boar semen. The results showed that 9 proteins were upregulated and 67 proteins were downregulated in frozen-thawed samples. These proteins are involved in oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Frederic Fournelle, Nidia Lauzon, Ethan Yang, Pierre Chaurand
Summary: Traditionally, MALDI IMS requires the deposition of an organic matrix, which often generates high background noise in the low mass range. LDI offers selective ionization with low background in the low mass range. This article discusses the advantages and disadvantages of solvent free and wet deposition of metals as LDI matrices and demonstrates the power of metal-assisted LDI for analyzing low molecular weight molecules in tissue sections.
MICROCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Peiqi Luo, Liping Wang, Lixia Jiang, Jie Sun, Yafeng Li, Huihui Liu, Caiqiao Xiong, Zongxiu Nie
Summary: Graphdiyne, a new carbon nanomaterial, has been utilized as a matrix in laser desorption ionization mass spectrometry for small molecule analysis, showing enhanced desorption and ionization efficiency. The use of Graphdiyne in LDI MS analysis can potentially improve the early diagnosis of liver cancer by identifying fatty acids as potential biomarkers.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Physics, Multidisciplinary
Meetu Luthra, Abhishek Prashant, Kanupriya Goswami, Anand Bharadvaja, Kasturi Lal Baluja
Summary: Partial ionisation cross-sections for cations formed by electron-impact dissociative ionisation of ethanol are calculated using a variant of the binary-encounter-Bethe (BEB) model. These cross-sections are crucial for modeling this process at a wide range of energies, as ethanol is an important molecule in astrophysical and combustion plasma. These theoretical calculations provide the only validation for experimental data and the simple approach used can estimate cross-section data for any molecule or its cation, making it highly valuable in understanding electron-molecule interactions.
PRAMANA-JOURNAL OF PHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Jacob R. Hauser, Edmund T. Bergstrom, Alexander N. Kulak, Stuart L. Warriner, Jane Thomas-Oates, Robin S. Bon
Summary: A series of pyrene-based LDI reagents (LALDI tags) have been developed for labeling and analyzing reducing carbohydrates in complex biological samples without the need for additional chemical derivatization or purification. The study demonstrates the potential of LALDI-MS for glycomics and diagnostics by direct detection of lactose in cow's milk.
Article
Biochemical Research Methods
Katrina Carbonara, Matthew P. Padula, Jens R. Coorssen
Summary: Integrative top-down proteomics aims to comprehensively analyze the breadth and depth of proteomes. This study disproves long-held beliefs about protein losses during front-end proteome resolution and confirms that standard facedown rehydration method has minimal impact on overall proteome resolution. This research demonstrates the importance of quantitative front-end 2DE/LC/MS/MS in analyzing proteomes by resolving their constituent proteoforms.
Article
Multidisciplinary Sciences
Lacey Johnson, Pearl Lei, Lauren Waters, Matthew P. Padula, Denese C. Marks
Summary: The aim of this study was to define the phenotype of in vitro generated platelet subpopulations and identify the subpopulations present in cryopreserved components. The study found that cryopreserved platelets contained procoagulant platelets and a novel population that did not align with the phenotype of aggregatory or apoptotic subpopulations.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Oliver McNeilly, Riti Mann, Max Laurence Cummins, Steven P. Djordjevic, Mehrad Hamidian, Cindy Gunawan
Summary: Several recent studies have found that bacteria are developing resistance to broad-spectrum antimicrobial silver nanoparticles (nanosilver; NAg). This is concerning because NAg is a crucial alternative antimicrobial agent. The present study focuses on the development of resistance to NAg in the globally prevalent bacterial pathogen Acinetobacter baumannii. The findings highlight the risks of long-term NAg use on a priority pathogen and emphasize the need for strategies to overcome bacterial NAg adaptation and preserve the efficacy of this potent antimicrobial agent in the era of antimicrobial resistance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
David S. Gertner, David P. Bishop, Matthew P. Padula
Summary: Phosphatidylinositols and phosphatidylinositol phosphates are closely related lipids that have been found to impact various cellular functions and are correlated with the development and progression of diseases. The analysis of these compounds is challenging due to their unique chemical characteristics, and current lipidomics methods are unsuitable. In this study, a new method was developed to enable the sensitive and simultaneous analysis of phosphatidylinositols and phosphatidylinositol phosphates, allowing for the identification of 148 species. This analysis was able to differentiate four distinct canola cultivars based on their phosphatidylinositide-lipidome, suggesting the potential use of lipidomic profiles in studying disease development and progression.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Cell Biology
Ben Winskel-Wood, Matthew P. Padula, Denese C. Marks, Lacey Johnson
Summary: Cryopreservation significantly alters the phenotype of platelets, generating distinct subpopulations. This study found that cryopreserved platelets exhibited increased adherence to a monocyte-like cell line and increased formation of platelet-leukocyte aggregates. The interaction between cryopreserved platelets and THP-1 cells was largely due to an increase in procoagulant platelets. Rating: 8/10.
Article
Genetics & Heredity
Paarthiphan Elankumuran, Glenn F. Browning, Marc S. Marenda, Amanda Kidsley, Marwan Osman, Marisa Haenni, James R. Johnson, Darren J. Trott, Cameron J. Reid, Steven P. Djordjevic
Summary: ST372 is the major Escherichia coli sequence type in dogs globally and is sporadically responsible for extraintestinal infections in humans. However, it is unclear whether ST372 strains from dogs and humans represent shared or distinct populations. In this study, the authors conducted bioinformatics analyses on 407 ST372 E. coli whole-genome sequences to characterize their epidemiological features, population structure, and associated accessory genomes. The results confirm that dogs are the dominant host of ST372, with distinct clusters within the population structure exhibiting different O:H types. Evidence of transmission between dogs and humans within different clusters was also found.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Max L. Cummins, Dmitriy Li, Aeman Ahmad, Rhys Bushell, Amir H. Noormohammadi, Dinidu S. Wijesurendra, Andrew Stent, Marc S. Marenda, Steven P. Djordjevic
Summary: Bacterial chondronecrosis with osteomyelitis (BCO) is a problem in the poultry industry, but its pathogenesis is not well-studied. This study analyzed 205 APEC(BCO) genome sequences and found that APEC(BCO) and APEC(colibac) are similar in phylogeny and genotype. APEC(BCO) frequently carry ColV-like plasmids with similar VAGs as APEC(colibac). The study provides insights for the management and treatment of lameness in poultry.
Article
Infectious Diseases
Yohannes E. Messele, Darren J. Trott, Mauida F. Hasoon, Tania Veltman, Joe P. McMeniman, Stephen P. Kidd, Steven P. Djordjevic, Kiro R. Petrovski, Wai Y. Low
Summary: The genetic characteristics and phylogenetic relationships among Escherichia coli isolates from cattle, pigs, poultry, and humans were analyzed using whole genome sequencing data. Most cattle and pig isolates belonged to phylogroups A and B1, while avian and human isolates belonged to B2 and D. Some cattle isolates carried extended-spectrum and AmpC beta-lactamase genes. The results indicate that cattle isolates from feedlots pose a reduced risk of being a source of clinically important antimicrobial-resistant E. coli.
Article
Biochemical Research Methods
Lana Brockbals, Samara Garrett-Rickman, Shanlin Fu, Maiken Ueland, Dennis McNevin, Matthew P. Padula
Summary: The aim of this study was to investigate the human decomposition process up to 3 months after death and propose novel time-dependent biomarkers for the estimation of decomposition time. An untargeted liquid chromatography tandem mass spectrometry-based bottom-up proteomics workflow was utilized to analyze skeletal muscle, and multiple peptide ratios were successfully proposed as a first step towards generalized, objective biochemical estimation of decomposition time.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Neus Gomila Pelegri, Aleksandra M. M. Stanczak, Amy L. L. Bottomley, Bruce K. K. Milthorpe, Catherine A. A. Gorrie, Matthew P. P. Padula, Jerran Santos
Summary: Neurological diseases are a major cause of disability and death worldwide, but effective treatments are still difficult to develop. Tissue engineering provides a potential solution for testing treatments, but creating accurate brain tissue models remains challenging. This study focused on using adipose-derived stem cells (ADSCs) to create neural models and found that growing ADSCs in a 3D environment with similar mechanical properties to the central nervous system can promote spontaneous neural differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Tamara Alhamami, Piklu Roy Chowdhury, Henrietta Venter, Tania Veltman, Alec Truswell, Sam Abraham, Sylvia A. Sapula, Mandi Carr, Steven P. Djordjevic, Darren J. Trott
Summary: Pasteurella multocida isolates from Australian feedlot cattle with bovine respiratory disease exhibited significant genetic diversity, with different capsular serogroups, lipopolysaccharide genotypes, and sequence types. Additionally, antibiotic resistance was predominantly observed in certain sequence types. The study provides insights into the genomic diversity and antimicrobial resistance associations of P. multocida in Australia.
VETERINARY MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Dmitriy Li, Paarthiphan Elankumaran, Timothy Kudinha, Amanda K. Kidsley, Darren J. Trott, Veronica Maria Jarocki, Steven Philip Djordjevic
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) are the most frequent cause of urinary tract infections (UTIs) globally. Whole genome sequencing (WGS) of E. coli isolates from a regional hospital in Australia in 2006 revealed several common sequence types and identified specific virulence plasmids. In addition, closely related isolates from the same patient associated with different pathologies and evidence of strain-sharing events involving isolates sourced from companion and wild animals were identified.
MICROBIAL GENOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Claire Rennie, Yanan Huang, Prakriti Siwakoti, Ziqing Du, Matthew Padula, Guochen Bao, Bernard E. Tuch, Xiaoxue Xu, Lana McClements
Summary: This study developed a core-shell nanostructure for controlled release of an immunomodulatory agent, MCC950, which effectively prevents fibrosis, inflammation, and monocyte adhesion caused by insulin-secreting cell implants. The nanosystem can be used in combination with insulin-secreting cell implants to increase their lifespan and improve the treatment of Type 1 diabetes mellitus.
Article
Biochemistry & Molecular Biology
Neus Gomila Pelegri, Aleksandra M. Stanczak, Amy L. Bottomley, Max L. Cummins, Bruce K. Milthorpe, Catherine A. Gorrie, Matthew P. Padula, Jerran Santos
Summary: This study cultured human adipose-derived stem cells (ADSCs) in 1.1 kPa polyethylene-glycol 3D hydrogels and examined the neural differentiation potential of ADSCs using B27, CultureOne (C1), and N2 neural supplements. The results showed that B27 and C1 enhanced the expression of neural markers in ADSCs, while N2 did not. Additionally, there were morphological changes observed in 2D environments but not in 3D environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Thomas Goulden, Iurii Bodachivskyi, Matthew P. Padula, D. Bradley G. Williams
Summary: The use of concentrated ionic liquids (ILs) in protein bioanalytical chemistry allows for improved sample preparation and analysis. Hydrophilic ILs show the best solubilisation capacity, and higher temperatures can enhance solubility but also reduce protein molecular weight.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)