Article
Chemistry, Multidisciplinary
Wen Yang, Lin Wang, Mulin Fang, Vinit Sheth, Yushan Zhang, Alyssa M. Holden, Nathan D. Donahue, Dixy E. Green, Alex N. Frickenstein, Evan M. Mettenbrink, Tyler A. Schwemley, Emmy R. Francek, Majood Haddad, Md Nazir Hossen, Shirsha Mukherjee, Si Wu, Paul L. DeAngelis, Stefan Wilhelm
Summary: In this study, we explored the use of self-polysaccharide heparosan (HEP) as a surface modification for nanoparticles. Our findings showed that HEP-coating reduced the protein corona formation and increased the uptake of nanoparticles by certain innate immune cells without causing cytotoxicity.
Article
Engineering, Biomedical
Clyde Overby, Soomin Park, Austin Summers, Danielle S. W. Benoit
Summary: In this study, diverse zwitterionic peptides (ZIPs) were developed and characterized for anti-fouling surface functionalization of nanoparticles (NPs). The results showed that the adsorption of proteins to NPs depends not only on the specific composition of the ZIPs, but also on the sequence and order of charges along the sequence (charge motif). These findings provide new tools for controlling protein adsorption profiles on ZIP-conjugated NPs and investigating the relationship between protein corona and biological function.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yajuan Zou, Shinji Ito, Masazumi Fujiwara, Naoki Komatsu
Summary: This study investigates the role of charged functional groups on the interaction between nanoparticles and cells. The protein affinity of different charged groups affects the cellular uptake efficiency and aggregation state of nanoparticles. The protein corona on the nanoparticle surface determines the uptake mechanism.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Feng Ge, Jianfeng Xue, Yi Du, Yan He
Summary: This study revealed that unmodified gold nanorods undergo an initial-adsorption stage and a membrane-diffusion stage on the cell membrane, with the driving force of interaction changing from electrostatic to ligand-receptor interaction, leading to the recruitment of protein ligands and making the nanoparticles cytofriendly.
Article
Biochemical Research Methods
Suman Pal, Ina F. de la Fuente, Shraddha S. Sawant, Jenna N. Cannata, Wu He, Jessica L. Rouge
Summary: In this study, the mechanisms and effects of serum on the uptake of DNA-functionalized micelles by cells were investigated. It was found that scavenger receptor-mediated, caveolae-dependent endocytosis is the major uptake pathway. Additionally, energy-independent pathways and clathrin-mediated endocytosis were involved in the uptake of particles degraded by enzymes. This study provides insights into the cytosolic delivery and therapeutic activity of DNA-functionalized nanomaterials, and highlights the stability of nucleic acids delivered by the micellular NAN platform in the presence of serum.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Russul Al-Nakashli, Radhika Raveendran, Yee Yee Khine, Cheng Cao, Sophie McKay, Hongxu Lu, Martina H. Stenzel
Summary: This study found that drug loading has an impact on cellular uptake of nanoparticles, which has been overlooked in previous studies focusing on properties such as shape and size. With increasing drug loading content, there was greater dehydration of the polymer shell, leading to increased protein adsorption and particle aggregation. The nanoparticle with the highest drug loading content showed reduced cellular uptake and toxicity in various cell lines. It was concluded that an intermediate drug loading content allows for sufficient cellular uptake and toxic drug concentration.
MOLECULAR PHARMACEUTICS
(2023)
Article
Nanoscience & Nanotechnology
Julie A. Champion, Thomas Pho
Summary: By altering the surface properties of protein nanoparticles, their interactions with mucosal barriers can be modified, affecting processes such as diffusion, cellular uptake, and immune cell maturation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Haijun Ma, Xiaoyan Zhang, Yang Yang, Shumu Li, Jiawei Huo, Yang Liu, Mirong Guan, Mingming Zhen, Chunying Shu, Jie Li, Chunru Wang
Summary: This study found that hydrophilic fullerene derivatives with reversed surface charges in aqueous solutions show remarkable performance in various biological applications, with C-60-EDA exhibiting stronger antioxidation capabilities due to its faster cellular uptake rate and mitochondrial enrichment compared to C-60-(EDA-EA).
Article
Nanoscience & Nanotechnology
Julien Dembele, Jou-Hsuan Liao, Tsang-Pai Liu, Yi-Ping Chen
Summary: This study presents a novel protein delivery approach using mesoporous silica nanoparticles, which enables effective intracellular delivery of denatured SOD protein and enhances its enzymatic activity. The strategy also allows for changes in corona protein composition of the nanoparticles for improved cytosolic delivery efficiency, while maintaining protein structure integrity.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
J. Oberlaender, C. Champanhac, R. da Costa Marques, K. Landfester, V Mailaender
Summary: This study investigated the influence of surface charge, temperature, and plasma concentration on the formation and composition of the protein corona of polystyrene nanoparticles and its impact on cellular uptake. The results showed that the composition of the corona is influenced by these parameters and can affect the cellular uptake of pre-coated nanoparticles.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Ana Mateos-Maroto, Meiyu Gai, Maximilian Brueckner, Richard da Costa Marques, Iain Harley, Johanna Simon, Volker Mailaender, Svenja Morsbach, Katharina Landfester
Summary: This study investigates the interaction between liposomes and cell membranes and how it affects their cellular uptake. The researchers found that by adjusting the lipid composition of liposomes, they could enhance their internalization by dendritic cells. This finding is significant for the design of specific drug carriers.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Chan Wang, Mengdie Xue, Xiao Liu, Jingjing Chen, Mengdie Jiang, Liuting Zheng, Ruxuan Ma, Chengjin Ding, Yaping Tao, Hao Zhang, Qin Liu, Da Huo
Summary: This study challenges the traditional view of protein corona effect as a hindrance to drug delivery efficacy, showing that it can actually enhance delivery and reduce toxicity when used strategically. By utilizing depleted serum to create functional protein coronas on nanomaterials, it was found that the passivation process could improve therapeutic effects and decrease adverse reactions. Moreover, this approach showed promising results in cancer treatment, offering a potential strategy for precision nanomedicine development.
Article
Engineering, Environmental
Zixuan Liu, Qiushuang Zhu, Erqun Song, Yang Song
Summary: This study investigated the protein corona formed on PM2.5 particles in biological fluids and found differences in corona composition between plasma and after BALF exposure. The results showed an abundance of serum albumin, hemoglobin, and fibrinogen proteins in the corona, and highlighted the contributions of various types of interactions in the adsorption process. Additionally, the study demonstrated that the protein corona could affect cellular internalization of PM2.5 and subsequent oxidative stress responses.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Analytical
Joanna Xiuzhu Xu, Md Siddik Alom, Nicholas C. Fitzkee
Summary: An effective intensity-based reference using N-15 tryptophan as an external reference for H-1-N-15 2D NMR experiments is presented in this study to quantify competitive protein adsorption onto nanoparticle surfaces. The referencing system validated the binding capacity of single protein onto gold NPs and accurately quantified signals from protein mixtures. The proposed Trp reference enables the monitoring of competitive adsorption of different proteins in situ and in real time, highlighting the importance of studying protein-NP interactions in protein mixtures using H-1-N-15 2D NMR techniques.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Multidisciplinary
Daniel Nierenberg, Orielyz Flores, David Fox, Yuen Yee Li Sip, Caroline M. Finn, Heba Ghozlan, Amanda Cox, Melanie Coathup, Karl Kai McKinstry, Lei Zhai, Annette R. Khaled
Summary: Pre-coating nanoparticles with sera from infected mice can enhance their uptake by cancer cells. The absorbed macromolecules from the sera modulate the biological identity of nanoparticles, achieving targeted uptake by specific cell types.
Article
Nanoscience & Nanotechnology
Jeongeun Kim, Sunay V. Chankeshwara, Frank Thielbeer, Jiyoung Jeong, Ken Donaldson, Mark Bradley, Wan-Seob Cho
Meeting Abstract
Respiratory System
A. R. Akram, N. Avlonitis, M. Vendrell, S. Chankeshwara, N. McDonald, T. Aslam, E. Scholefield, T. Walsh, C. Haslett, M. Bradley, K. Dhaliwal
Article
Chemistry, Multidisciplinary
Ahsan R. Akram, Nicolaos Avlonitis, Annamaria Lilienkampf, Ana M. Perez-Lopez, Neil McDonald, Sunay V. Chankeshwara, Emma Scholefield, Christopher Haslett, Mark Bradley, Kevin Dhaliwal
Article
Chemistry, Multidisciplinary
Tashfeen Aslam, Amy Miele, Sunay V. Chankeshwara, Alicia Megia-Fernandez, Chesney Michels, Ahsan R. Akram, Neil McDonald, Nik Hirani, Chris Haslett, Mark Bradley, Kevin Dhaliwal
Article
Chemistry, Multidisciplinary
Eugenio Indrigo, Jessica Clavadetscher, Sunay V. Chankeshwara, Annamaria Lilienkampf, Mark Bradley
CHEMICAL COMMUNICATIONS
(2016)
Article
Environmental Sciences
Sung-Hyun Hwang, Frank Thielbeer, Jiyoung Jeong, Youngju Han, Sunay V. Chankeshwara, Mark Bradley, Wan-Seob Cho
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
(2016)
Correction
Respiratory System
A. R. Akram, N. Avlonitis, M. Vendrell, S. Chankeshwara, N. McDonald, T. Aslam, E. Scholefield, T. Walsh, C. Haslett, M. Bradley, K. Dhaliwal
Article
Chemistry, Organic
Alicia Megia-Fernandez, Bethany Mills, Chesney Michels, Sunay V. Chankeshwara, Kevin Dhaliwal, Mark Bradley
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Review
Biochemistry & Molecular Biology
Sunay V. Chankeshwara, Eugenio Indrigo, Mark Bradley
CURRENT OPINION IN CHEMICAL BIOLOGY
(2014)
Article
Chemistry, Organic
Alicia Megia-Fernandez, Bethany Mills, Chesney Michels, Sunay V. Chankeshwara, Nikola Krstajic, Chris Haslett, Kevin Dhaliwal, Mark Bradley
ORGANIC & BIOMOLECULAR CHEMISTRY
(2018)
Article
Cell Biology
Ahsan R. Akram, Sunay V. Chankeshwara, Emma Scholefield, Tashfeen Aslam, Neil McDonald, Alicia Megia-Fernandez, Adam Marshall, Bethany Mills, Nicolaos Avlonitis, Thomas H. Craven, Annya M. Smyth, David S. Collie, Calum Gray, Nik Hirani, Adam T. Hill, John R. Govan, Timothy Walsh, Christopher Haslett, Mark Bradley, Kevin Dhaliwal
SCIENCE TRANSLATIONAL MEDICINE
(2018)
Article
Chemistry, Multidisciplinary
Soyeon Jeon, Jessica Clavadetscher, Dong-Keun Lee, Sunay Chankeshwara, Mark Bradley, Wan-Seob Cho
Proceedings Paper
Optics
Tushar R. Choudhary, Michael G. Tanner, Alicia Megia-Fernandez, Kerrianne Harrington, Harry A. Wood, Sunay Chankeshwara, Patricia Zhu, Debaditya Choudhury, Fei Yu, Robert R. Thomson, Rory R. Duncan, Kevin Dhaliwal, Mark Bradley
OPTICAL FIBERS AND SENSORS FOR MEDICAL DIAGNOSTICS AND TREATMENT APPLICATIONS XVII
(2017)
Article
Chemistry, Multidisciplinary
Eugenio Indrigo, Jessica Clavadetscher, Sunay V. Chankeshwara, Alicia Megia-Fernandez, Annamaria Lilienkampf, Mark Bradley
CHEMICAL COMMUNICATIONS
(2017)
Article
Chemistry, Multidisciplinary
Jessica Clavadetscher, Eugenio Indrigo, Sunay V. Chankeshwara, Annamaria Lilienkampf, Mark Bradley
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
