Journal
MACROMOLECULAR BIOSCIENCE
Volume 13, Issue 4, Pages 422-436Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201200255
Keywords
atomic force microscopy (AFM); nonviral gene vectors; polyamidoamine (PAMAM); polyethyleneimine (PEI); self-assembly
Funding
- National Natural Science Foundation of China [30870618, 31170918]
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A new derivative of polyamidoamine and polyethylenimine, G2.5-PEI 423 or G1.5-PEI 423, is prepared by an amidation reaction of PAMAM G2.5 or PAMAM G1.5 using PEI 423. The polycations show a great ability to combine with pDNA to form complexes, which protect the pDNA from nuclease degradation. The polymers display stronger buffer capacity and lower cytotoxicity. The complexes have particle sizes of 120180nm and zeta potentials of 2040mV. The G2.5-PEI 423 complexes display much higher transfection efficiencies than PAMAM G5 and Lipo-2k, and the G1.5-PEI 423 complexes display higher transfection efficiencies than PAMAM G4 and PEI-25k. The complexes possess better serum-resistant capacity. The G2.5-PEI 423 has a great potential to be used as a serum-resistant gene vector.
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