Journal
MACROMOLECULAR BIOSCIENCE
Volume 13, Issue 6, Pages 717-722Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201200469
Keywords
affinity chromism; conjugated polymers; DNA; oligonucleotides; polythiophenes
Funding
- Defence Research and Development Canada Centre for Security Science Chemical, Biological, Radiological/Nuclear, and Explosives Research and Technology Initiative [CRTI-03-0005RD, CRTI-06-0186RD, CRTI-06-0187TD]
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DNA hybridization is a universal and specific mechanism for the recognition of biological targets. Some cationic polythiophene transducers sensitive to DNA structure have been previously utilized to detect such biomolecules. Further characterization of these systems indicates that both DNA sequence composition and length modulate the biosensor performance. It appears that different repeated sequence patterns cause different conformational changes of the polythiophene, from a more relaxed form to an extremely rigid one. A length difference between the DNA oligonucleotide probe and target has a detrimental effect on the fluorescent signal, but it can be attenuated by changing the sequence composition of the protruding target sequence. This demonstrates that the nature of DNA can be critical for hybridization-based detection systems.
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