Journal
MACROMOLECULAR BIOSCIENCE
Volume 12, Issue 9, Pages 1232-1242Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201200095
Keywords
biomimetic modifications; click chemistry; peptide radiolabeling; polydopamine; poly(ethylene oxide)
Funding
- Grant Agency of the Academy of Sciences CR [KJB400500904]
- Grant Agency of the Czech Republic [P108/11/1857, AV0Z50390703]
- Ministry of Education, Youth and Sports [1M0538, MSM0021622430]
- Masaryk University [MUNI/A/0933/2010]
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A simple, versatile, protein-repulsive, substrate-independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow-up modifications with various ligands using a copper-catalyzed alkyne-azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125I-radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line.
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