Journal
MACROMOLECULAR BIOSCIENCE
Volume 12, Issue 4, Pages 533-546Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201100394
Keywords
biological applications of polymers; endotoxin removal; PEGylation; step-growth polymerization; thermal click chemistry
Funding
- National Natural Science Foundation of China [20774094, 51021003, 50733003, 20874097]
- Ministry of Science and Technology of China [2009CB930102]
- Chinese Academy of Sciences [KGCX2-YW-802]
- Jilin Provincial Science and Technology Department [20082104, 20100588]
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PEGylated click polypeptides (PEG-CPs) containing a-amino side groups as well as PEG segments are designed for selective endotoxin removal from protein solutions. The PEG-CPs are synthesized via copper-free thermal click copolymerization from aspartic (or glutamic) acid-based dialkyne and diazide monomers (containing free amino side groups) and alkyne-terminated mPEGs or dialkyne-terminated PEGs. Microwave-assisting technology is introduced into thermal click chemistry to improve the reaction efficiency. The monomers and polymers are fully characterized using NMR, XPS, and MALDI-TOF MS. After immobilizing the PEGylated click polypeptides onto polystyrene microspheres, the adsorbents exhibit good endotoxin removal selectivity from BSA solutions.
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