Article
Food Science & Technology
Nan Yang, Xiaolong Liang, Jinxuan Cao, Qian Zhang, Yongzhao Tan, Beitao Xu, Yanling Yang, Yu Wang, Qingjin Yang, Hong Liu, Jiao Liu
Summary: The aim of this study was to investigate the denaturation mechanism of sarcoplasmic proteins (SP) under PSE condition. The results showed that PSE-denaturation and heat-denaturation of SP were different processes, and the improvement of water-holding capacity was observed only under PSE-like condition.
Article
Fisheries
Xiaotong Sun, Hongtao Nie, Xiwu Yan
Summary: This study identified and investigated three novel glycogen metabolism-related proteins in Manila clam, suggesting their potential involvement in innate immunity and important role in immune defense.
Article
Biochemistry & Molecular Biology
David L. Brautigan
Summary: Glycogen is a polymerized form of glucose used as an energy reserve in organisms. The synthesis and degradation of glycogen in animals, especially in the liver and skeletal muscle, is regulated by hormones and physiological signals that control the activities of glycogen synthase and glycogen phosphorylase. This article discusses the flash activation of phosphorylase in glycogen particles and suggests that the regulation of phosphatase in glycogen particles may involve hybrids of phosphorylase.
Article
Biochemistry & Molecular Biology
Izabela Gutowska, Agnieszka Maruszewska, Marta Skorka-Majewicz, Agnieszka Kempinska-Podhorodecka, Agnieszka Kolasa, Agata Wszolek, Irena Baranowska-Bosiacka, Wojciech Zwierello
Summary: Human exposure to fluorine from multiple sources can lead to metabolic disorders, particularly in muscle cells. This study investigated the impact of fluoride exposure on enzymes involved in muscle glycogen metabolism. It was found that fluoride exposure altered energy state, enzyme expression, and glycogen deposition in cells.
Article
Food Science & Technology
Petter Vejle Andersen, Nils Kristian Afseth, Eli Gjerlaug-Enger, Jens Petter Wold
Summary: This study investigated the potential of Raman spectroscopy to improve prediction of drip loss in pork by recording spectra at different positions. Results showed that the best predictions were achieved using spectra from the ventral plug, highlighting the importance of careful selection of spectral collection location for successful predictions.
Article
Pediatrics
Yongxian Shao, Taolin Li, Minyan Jiang, Jianan Xu, Yonglan Huang, Xiuzhen Li, Ruidan Zheng, Li Liu
Summary: This case report describes a patient with GSD-IXc who had two novel mutations in the PHKG2 gene. Functional experiments showed decreased enzyme activity due to these mutations. Treatment with raw cornstarch improved the patient's symptoms.
Review
Pediatrics
Zahra Beyzaei, Fatih Ezgu, Bita Geramizadeh, Alireza Alborzi, Alireza Shojazadeh
Summary: This case report describes a young girl diagnosed with GSD carrying two novel mutations related to GSD IXb type. Liver biopsy and targeted-gene sequencing identified pathogenic variants in the PHKB gene. The identification of novel variants in this patient expands the understanding of dual variants in GSD-IXb.
Article
Fisheries
Jibin Lin, Yanqin Liao, Xueshan Li, Kangle Lu, Kai Song, Ling Wang, Lu Zhang, Chunxiao Zhang
Summary: This study aimed to evaluate the effects of dietary creatine (CR) levels on the growth, muscle energy metabolism, and meat quality of spotted seabass. The results showed that increasing dietary CR levels promoted weight gain and improved the quality of the meat. The optimal CR supplementation level for spotted seabass was found to be 0.25%. Additionally, dietary CR supplementation enhanced energy reserve and decreased anaerobic glycolysis, leading to improved meat quality.
Article
Cell Biology
Yanchao Liu, Shujuan Zhang, Benrong He, Liangkai Chen, Dan Ke, Shi Zhao, Yao Zhang, Wei Wei, Zhipeng Xu, Zihui Xu, Ying Yin, Wen Mo, Yanni Li, Yang Gao, Shihong Li, Weijin Wang, Huiling Yu, Dongqin Wu, Guilin Pi, Tao Jiang, Mingmin Deng, Rui Xiong, Huiyang Lei, Na Tian, Ting He, Fei Sun, Qiuzhi Zhou, Xin Wang, Jinwang Ye, Mengzhu Li, Nan Hu, Guoda Song, Wenju Peng, Chenghong Zheng, Huaqiu Zhang, Jian-Zhi Wang
Summary: Elevation of plasma A beta 1-42/A beta 1-40 is an independent risk factor of MCI in T2DM patients. Combining elevated A beta 1-42/A beta 1-40 with activated platelet GSK-3 beta, ApoE epsilon 4 genotype, olfactory decline, and aging increases the efficiency of MCI diagnosis in T2DM patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Julio Henrique Muzetti, Daniel Almeida do Valle, Mara L. S. Ferreira Santos, Bruno Augusto Telles, Mara L. Cordeiro
Summary: Glycogen storage diseases (GSD) are rare inherited diseases that affect glycogen metabolism, leading to hypoglycemia and potential neurological damage. This study aimed to investigate metabolic, genetic, and neurological profiles of children with GSD and found that GSD type I may have a greater neurological impact compared to GSD type IX. The study identified different mutations and highlighted the significance of adequate metabolic control in managing the neurological consequences of GSD.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biology
Oksana M. Sorochynska, Oksana V. Kuzniak, Maria M. Bayliak, Yulia V. Vasylyk, Kenneth B. Storey, Volodymyr I. Lushchak
Summary: The study compared the metabolic capabilities of skeletal hind limb muscles of one month old mice under restricted feeding (EODF) and ad libitum feeding (AL). Results showed lower concentrations of metabolic substrates in the muscles of EODF mice compared to AL mice.
Article
Multidisciplinary Sciences
Juliana C. Correa-Velloso, Paula J. Bartlett, Robert Brumer, Lawrence D. Gaspers, Henning Ulrich, Andrew P. Thomas
Summary: Rat hepatocytes express multiple purinergic P2Y and P2X receptors, leading to distinct Ca2+ oscillations. These oscillation patterns are shaped by protein kinase C, resulting in unique cellular responses to different agonists.
Article
Multidisciplinary Sciences
Kongphop Parunyakul, Krittika Srisuksai, Sawanya Charoenlappanit, Narumon Phaonakrop, Sittiruk Roytrakul, Wirasak Fungfuang
Summary: Cordycepin may help prevent hepatic metabolism in T1DM by regulating the expression of energy-related proteins and UPS to maintain cell survival, as observed in the study on streptozotocin-induced T1DM mice. The improvement in liver function with cordycepin treatment was related to changes in metabolic pathways and protein expression. Further research is needed to explore the therapeutic potential of cordycepin in metabolic mechanisms.
Article
Endocrinology & Metabolism
Rebecca A. Gibson, Jeong-A Lim, Su Jin Choi, Leticia Flores, Lani Clinton, Deeksha Bali, Sarah Young, Aravind Asokan, Baodong Sun, Priya S. Kishnani
Summary: This study presents the first evidence of a mouse model that recapitulates the liver-specific pathology of patients with GSD IX gamma 2, providing a valuable tool for further studying disease progression and evaluating novel therapeutics.
MOLECULAR GENETICS AND METABOLISM
(2021)
Review
Clinical Neurology
Madoka Mori-Yoshimura, Kazutaka Aizawa, Yasushi Oya, Yoshihiko Saito, Tokiko Fukuda, Hideo Sugie, Ichizo Nishino, Yuji Takahashi
Summary: PHKA1 mutations are causative for glycogen storage disease type IXd (GSDIXd), a myopathy that can manifest as asymptomatic or with exercise intolerance. This report describes a patient with GSDIXd who developed distal myopathy at the age of 71, without exercise intolerance. Muscle MRI revealed severe progressive involvement of muscles in the order of medial gastrocnemius, soleus, lateral gastrocnemius, and gluteus muscles. Muscle pathology showed vacuolar changes with glycogen accumulation, and the enzymatic activity of phosphorylase b kinase was significantly decreased.
NEUROMUSCULAR DISORDERS
(2022)
Article
Chemistry, Applied
Yuemei Zhang, Eero Puolanne, Per Ertbjerg
Summary: This study reveals that protein denaturation in freezing-thawing process is primarily caused by the combination of decreased pH and high solute concentrations in the unfrozen water of frozen meat, leading to changes in water-holding capacity and surface hydrophobicity of myofibrils.
Article
Food Science & Technology
Jian Lyu, Per Ertbjerg
Summary: The study revealed that an increase in Ca2+ concentration can activate calpain and induce its binding to myofibrils, thereby making it proteolytically active.
Article
Agriculture, Dairy & Animal Science
A. Conanec, M. Campo, I Richardson, P. Ertbjerg, S. Failla, B. Panea, M. Chavent, J. Saracco, J. L. Williams, M-P Ellies-Oury, J-F Hocquette
Summary: This study evaluated the impact of breed on the sensory quality of beef from longissimus muscle by rearing 436 young cattle from 15 breeds in similar conditions. Different statistical methods were compared, with the results showing that breed did impact beef flavor. The study found that certain breeds with higher lipid content in the muscle exhibited stronger beef flavor, while others produced less juicy and tender meat.
Article
Food Science & Technology
Elisa Dominguez-Hernandez, Per Ertbjerg
Summary: The study aimed to investigate biophysical and chemical changes during low-temperature long-time heat treatment of pork by analyzing cathepsin B+L activity, myofibril surface hydrophobicity, particle size of myofibrils, and meat toughness. Results showed that meat toughness decreased at lower temperatures and with prolonged cooking times, with changes in myofibrillar surface hydrophobicity and particle size associated with cathepsin B+L activity.
Article
Food Science & Technology
Seyedmahmood Razavizadeh, Gitana Alencikiene, Alvija Salaseviciene, Lina Vaiciulyte-Funk, Per Ertbjerg, Aelita Zabulione
Summary: This research investigated the effects of fermentation of ultrasound-treated okara on meat analogues, finding that fermentation improved sensory properties and reduced protein oxidation levels. The study recommended adding 6% fermented okara to matrices and fermenting at 4 degrees Celsius for optimal results.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Yuemei Zhang, Arianna Magro, Eero Puolanne, Antonella Dalle Zotte, Per Ertbjerg
Summary: This study found that freezing rate at -3°C had significant effects on myofibrillar proteins in the short term, but these effects gradually diminished and disappeared over time.
Article
Food Science & Technology
Binbin Li, Xinyue Dong, Eero Puolanne, Per Ertbjerg
Summary: This study found that wooden breast condition has a significant impact on lipid and protein oxidation as well as citrate synthase activity, worsening with the severity of the condition. Both bird age and wooden breast degree have main effects on these indicators, and there are significant correlations among them.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Jian Lyu, Per Ertbjerg
Summary: The study investigated the subcellular distribution of calpain-1 and -2 as well as the proteolytic activity of myofibril-bound calpains during 12 days cold storage in pork. The results showed that calpain-1 gradually translocated from sarcoplasm to myofibrils during the initial 6 days of postmortem storage, and both calpain-1 and calpain-2 were found to bind to myofibrils and subsequently degrade structural proteins, including desmin.
Article
Food Science & Technology
Seyedmahmood Razavizadeh, Gitana Alencikiene, Lina Vaiciulyte-Funk, Per Ertbjerg, Alvija Salaseviciene
Summary: The aim of this study was to improve the properties of soy press cake for use in meat analogues by fermentation and hydrolysis. The results showed that fermentation and enzymatic hydrolysis could enhance the water- and oil-holding capacity of soy press cakes and reduce protein oxidation. Fermented soy press cake also improved the texture and taste of meat analogues.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Food Science & Technology
Yuemei Zhang, Y. H. B. Kim, Eero Puolanne, Per Ertbjerg
Summary: The denaturation of myofibrillar proteins plays a crucial role in the formation of thaw loss during freezing-thawing process of meat. The decrease in pH and increase in ionic strength in the unfrozen water during freezing are hypothesized to cause protein denaturation and conformational changes, leading to loss of water-holding capacity. Slow freezing results in more thaw loss compared to fast freezing, indicating the impact of freezing rate on myofibrillar protein denaturation.
Article
Food Science & Technology
Yun-Fang Qian, Jing-Jing Zhang, Cheng-Cheng Liu, Per Ertbjerg, Sheng-Ping Yang
Summary: This study investigated the effect of gaseous ozone with different dose and exposing time on the quality of salmon. The results showed that ozone treatment reduced microbial growth, lipid oxidation, and sensory score decrease, and also eliminated fishy smell. Next-generation sequencing revealed that Photobacterium was the main bacteria causing deterioration of salmon fillets. Exposing time had a greater effect on salmon quality than ozone concentrations.
Article
Food Science & Technology
Yun-Fang Qian, Cheng-Cheng Liu, Jing-Jing Zhang, Per Ertbjerg, Sheng-Ping Yang
Summary: Modified atmosphere packaging (MAP) has effects on the texture, protein contents, and odor characteristics of salmon. The combination of 60%CO2 / 10%O-2 / 30%N-2 shows the best results in preventing protein degradation and odor changes.
Article
Food Science & Technology
Jian Lyu, Eero Puolanne, Per Ertbjerg
Summary: The study investigated the impact of pre-rigor temperature incubation on calpain activity and distribution in sarcoplasmic and myofibrillar fractions, as well as meat quality attributes. Higher pre-rigor temperature led to increased sarcoplasmic calcium concentration, purge loss, and myofibril-bound calpain-1 content, while shear force decreased. Pre-rigor incubation at 38 degrees C resulted in lower water-holding capacity of isolated myofibrils. The presence of sufficient calcium indicated that calpain-1 and calpain-2 were associated with myofibrils and proteolytically active.
Article
Food Science & Technology
Xueshen Zhu, Eero Puolanne, Per Ertbjerg
Summary: The wooden breast syndrome significantly affects the texture, water loss, and thermal properties of broiler meat, but these effects decrease over the early postmortem period (1-3 days).
Article
Food Science & Technology
Binbin Li, Jere Linden, Eero Puolanne, Per Ertbjerg
Summary: This study investigates the effects of wooden breast syndrome on protein traits and total myofiber area in chicken pectoralis major muscle. The results show that the content of sarcoplasmic and myofibrillar proteins decreases with increasing severity of wooden breast syndrome, while the amount of collagen and protein denaturation, as well as the area of degenerated myofibers, connective tissue, and cellular infiltrates, increases.
