Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 59, Issue 1, Pages 300-307Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2014.04.052
Keywords
Lectin; Phytohemagglutinin; Red kidney bean; Gamma irradiation; Mitogen
Categories
Ask authors/readers for more resources
Gamma radiation inactivation of phytohemagglutinin (PHA) of red kidney bean in purified form as well as in seeds in different moisture conditions was demonstrated at different doses viz. 1, 10, 30, 50, 100 and 200 kGy. Irradiation of PHA in dry state (99.5% dry) or in 50% moisture condition showed structural intactness as studied by SDS PAGE, size exclusion chromatography (SEC), fluorescence measurement. CD spectroscopy below 30 kGy, while a 50% reduction in hemagglutinin and mitogenic activity was observed in the dose range of 30-50 kGy. Radiation inactivation of purified PHA was more pronounced when irradiated in aqueous solution form resulting in complete destruction of secondary and tertiary structure as well as function at a dose of 10 kGy. Radiation processing of dry and soaked seeds of kidney bean exhibited 50% loss in functional activity of PHA at the doses of 50 and 30 kGy, respectively. Practicality of implication of the radiation processing for inactivation of this antinutrient in legume seeds is discussed. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available