Analysis of fexofenadine in pharmaceutical formulations using tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate chemiluminescence system in a multichip device

A simple, rapid and sensitive method has been developed for the analysis of fexofenadine (FEX) in pharmaceutical formulations, using a tris(1,10-phenanthroline)ruthenium(II) [Ru(phen)(3)(2+)] peroxydisulphate chemiluminescence (CL) system in a multichip device. Various parameters that influence the CL signal intensity were optimized. These included pH, flow rates and concentration of reagents used. Under optimum conditions, a linear calibration curve in the range 0.05-5.0 mu g/mL was obtained. The detection limit was found to be 0.001 mu g/mL. The procedure was applied to the analysis of FEX in pharmaceutical products and was found to be free from interference from concomitants usually present in these preparations. Copyright (C) 2011 John Wiley & Sons, Ltd.