Article
Biochemistry & Molecular Biology
Shuai Wang, Panpan Tan, Hongwei Wang, Jicang Wang, Cai Zhang, Hao Lu, Baoyu Zhao
Summary: Swainsonine (SW) is a primary toxin in locoweed. This study reveals that SW inhibits autophagy degradation by affecting O-GlcNAcylation modification process, leading to cytotoxicity. These findings provide a new theoretical basis for the toxicological mechanism of SW.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Cell Biology
M. Iqbal Hossain, Joshua M. Marcus, Jun Hee Lee, Patrick L. Garcia, VinodKumar Singh, John J. Shacka, Jianhua Zhang, Toby Gropend, Charles N. Falany, Shaida A. Andrabi
Summary: The study reveals that the lack of CTSD protein in cerebral ischemia leads to lysosomal dysfunction and neuronal cell death. Restoring CTSD protein levels increases neuronal resilience against cell death by maintaining lysosomal function.
Article
Medicine, Research & Experimental
Xiang Wang, Yan Zou, Zhidan Chen, Yang Li, Le Pan, Ying Wang, Ming Liu, Chao Yin, Jian Wu, Chunjie Yang, Lei Zhang, Chenze Li, Zheyong Huang, Daowen Wang, Juying Qian, Junbo Ge, Yunzeng Zou, Hui Gong
Summary: The study showed that cardiomyocyte-expressed LRP6 inhibits cardiac fibrosis by promoting the degradation of Wnt5a and Wnt11 under chronic pressure overload, highlighting a protective role in heart function.
Article
Agronomy
Piotr Cybulski, Magdalena Larska, Aleksandra Wozniak, Artur Jablonski, Tomasz Stadejek
Summary: The study revealed that increasing protein level can significantly raise the occurrence of gastric ulcers, while adding wheat bran and liquid feeding may have the opposite effect.
Article
Chemistry, Applied
Ziwei Wang, Kunying Chen, Ke Zhang, Kaihong He, Duoduo Zhang, Xiaohan Guo, Tongwen Huang, Jielun Hu, Xingtao Zhou, Shaoping Nie
Summary: ACP inhibits proliferation of colorectal cancer cells by inducing apoptosis and lysosomal dysfunction through regulation of mitochondria, lysosome, and apoptosis-related pathways. It also increases histone H3-lysine-27 acetylation (H3K27ac) on CTSD promoter, facilitating TFEB binding and CTSD expression. ACP has potential as an anti-cancer agent for colorectal cancer treatment.
CARBOHYDRATE POLYMERS
(2023)
Article
Cell Biology
Yu-Qin Di, Xiao-Lin Han, Xin-Le Kang, Di Wang, Cai-Hua Chen, Jin-Xing Wang, Xiao-Fan Zhao
Summary: CTSD has dual functions in promoting cell proliferation and apoptosis, with its secretion determined by N233 glycosylation and expression promoted by 20E hormone. Autophagy triggers its maturation and localization inside cells, leading to activation of CASP3 and apoptosis. Its differential expression in tissues plays a role in determining cell fates during lepidopteran metamorphosis.
Article
Agriculture, Dairy & Animal Science
Anna Koseniuk, Grzegorz Smolucha, Malgorzata Natonek-Wisniewska, Anna Radko, Dominika Rubis
Summary: In this study, the researchers aimed to differentiate wild boars from domestic pigs by analyzing the polymorphisms within the MC1R and NR6A1 genes. They used PCR-RFLP and Real-time PCR techniques to study samples from wild boars in Poland and five pig breeds. The results showed that the NR6A1 g.748C > T variation is an effective marker for distinguishing between wild boars and domestic pigs. The study also revealed admixture between the two genomes using population genetics software.
Article
Agriculture, Dairy & Animal Science
Hao Liu, Xing Zhang, Yaning Hu, Xingbo Zhao
Summary: This study reveals an association between mtDNA polymorphisms and backfat thickness (BFT) in pigs. The 48 polymorphic sites in mtDNA generated 22 haplotypes, which clustered into 4 haplogroups. HG1 showed a lower BFT value compared to other haplogroups, and H4 in HG1 exhibited the lowest BFT of all analyzed haplotypes.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Worrarak Norseeda, Guisheng Liu, Tawatchai Teltathum, Pantaporn Supakankul, Korawan Sringarm, Watcharapong Naraballobh, Trisadee Khamlor, Siriwadee Chomdej, Korakot Nganvongpanit, Patcharin Krutmuang, Supamit Mekchay
Summary: The IL-4 and IL-4R cytokine genes play a role in the immune and reproductive systems of pigs, and their polymorphisms have been found to be associated with litter size traits. These genetic markers could potentially be used to improve the reproductive traits of pigs in the breeding industry.
Article
Agriculture, Dairy & Animal Science
Grzegorz Sieminski, Piotr Skalecki, Mariusz Florek, Piotr Domaradzki, Ewa Poleszak, Malgorzata Dmoch, Malgorzata Ryszkowska-Siwko, Monika Kedzierska-Matysek, Anna Teter, Marek Kowalczyk, Agnieszka Kaliniak-Dziura
Summary: The old indigenous pig breeds in Poland play a vital role in preserving livestock biodiversity. Crossbreeds are used for commercial pig production. This study compared the nutritional quality and health-promoting properties of meat from Pulawska breed pigs and their crossbreeds. The Pulawska breed had the lowest intramuscular fat content, but also the lowest saturated fatty acids, most favorable PUFA/SFA ratio, and the best atherogenic and thrombogenic indices. It also had a high protein content and concentration of indispensable amino acids.
Article
Food Science & Technology
Hanna Jankowiak, Aleksandra Cebulska, Maria Bocian
Summary: The study shows a close relationship between pH values and quality characteristics of meat, with variations in pH impacting water-holding capacity, tenderness, color, and other properties of the meat.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Medicine, General & Internal
Justyna Magielda-Stola, Grazyna Kurzawinska, Marcin Ozarowski, Tomasz M. Karpinski, Krzysztof Drews, Agnieszka Seremak-Mrozikiewicz
Summary: This study aimed to investigate the association between VDR gene single-nucleotide polymorphisms and preeclampsia in the Polish population. The results revealed that the VDR BsmI AA genotype was more frequent in preeclamptic women and associated with a higher risk of PE. Additionally, the VDR BsmI A allele may be closely related to a higher predisposition to hypertension.
Article
Genetics & Heredity
Jiali Xu, Yong Ruan, Jinkui Sun, Pengfei Shi, Jiajin Huang, Lingang Dai, Meimei Xiao, Houqiang Xu
Summary: The study evaluated the growth traits of Xiangsu hybrid pigs through PRKAA2 and MSMB as candidate genes. Sanger sequencing identified three intronic mutation sites in PRKAA2 and six mutation sites (including both exonic and intronic) in MSMB. Association analysis showed different relationship patterns between these mutation sites and various growth traits. The expression of PRKAA2 was highest in the longissimus dorsi muscle, while the expression of MSMB was highest in the spleen. These findings suggest that PRKAA2 and MSMB can be used for marker-assisted selection in improving growth traits of Xiangsu hybrid pigs, contributing to pig molecular breeding.
Article
Agriculture, Dairy & Animal Science
Xiaoyan Wang, Enrico D'Alessandro, Chenglin Chi, Ali Shoaib Moawad, Wencheng Zong, Cai Chen, Chengyi Song
Summary: This study evaluates the genetic variation and population structure of native pig populations in Jiangsu Province, China using SINE-RIPs. The results show that the native pig populations in Jiangsu are more polymorphic compared to crossbreeds and external breeds, and these findings can aid in the conservation and utilization of Chinese native pigs.
Article
Dentistry, Oral Surgery & Medicine
Tomasz Olszowski, Marta Milona, Joanna Janiszewska-Olszowska, Krzysztof Safranow, Izabela Uzar, Alicja Walczak, Maciej Sikora, Dariusz Chlubek, Grazyna Adler
Summary: The study found that FCN1 SNPs are not associated with dental caries susceptibility in Polish children. There were no significant differences in the genotype, allele, or haplotype distributions of FCN1 SNPs between children with higher and lower caries experience.
Article
Agriculture, Dairy & Animal Science
Kamila Pokorna, Jaroslav Citek, Petr Dolezal, Martyna Malopolska, Miroslav Tyra, Monika Okrouhla, Katerina Zadinova, Michal Sprysl, Nicole Lebedova, Roman Stupka
Summary: This study focused on finding a non-invasive method to determine boar taint in pigs by evaluating the concentration of androstenone compounds in their saliva. The results showed a correlation between the concentration of salivary androstenone and the age of the boars. This research has significant implications for preventing the occurrence of boar taint in pork.
Article
Biochemistry & Molecular Biology
Przemyslaw Podstawski, Katarzyna Ropka-Molik, Ewelina Semik-Gurgul, Marcin Samiec, Maria Skrzyszowska, Zenon Podstawski, Tomasz Szmatola, Maciej Witkowski, Klaudia Pawlina-Tyszko
Summary: The extracellular matrix (ECM) plays a crucial role in tissue structure and biochemical reactions, and dysregulation of the ECM can lead to diseases such as cancer. This study investigated the expression profiles of genes related to ECM remodeling and cell adhesion in equine sarcoid, the most common skin cancer in horses. The results suggest that abnormal expression of ECM-related genes is correlated with sarcoid formation. Understanding the molecular pathways involved in ECM remodeling and cell adhesion could contribute to the development of biomarkers and potential therapeutic targets for sarcoid-related cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Katarzyna Piorkowska, Kacper Zukowski, Katarzyna Ropka-Molik, Miroslaw Tyra
Summary: The goal of this study was to evaluate the effects of mutations in the FGL1 gene associated with pig productive traits. More than 50 crucial mutations were identified in the FGL1 gene. The association study showed significant effects of the FGL1 on various traits related to fat deposition. The study supports the theory that liver FGL1 is involved in the fat deposition process and provides a potential genetic marker for pig selection.
Article
Agriculture, Dairy & Animal Science
Grzegorz Mycka, Weronika Klecel, Monika Stefaniuk-Szmukier, Joanna Jaworska, Adrianna Dominika Musial, Katarzyna Ropka-Molik
Summary: In this study, we investigated the genetic composition of the Polish draft horse (PDH) breed by analyzing whole mitochondrial d-loop variabilities and comparing them to sequences of other cold-blooded breeds. Our results showed high nucleotide diversity among the PDH population and the existence of two main haplogroups with strong kinship to the Belgian breed.
Article
Biochemistry & Molecular Biology
Ewa Tomaszewska, Malgorzata Swiatkiewicz, Siemowit Muszynski, Janine Donaldson, Katarzyna Ropka-Molik, Marcin B. Arciszewski, Maciej Murawski, Tomasz Schwarz, Piotr Dobrowolski, Sylwia Szymanczyk, Slawomir Dresler, Joanna Bonior
Summary: Chronic pancreatitis (CP) is an irreversible and progressive inflammatory disease. This study aimed to investigate the changes in pro-inflammatory cytokines, antioxidant defense system, and selected serum biochemical parameters in a cerulein-induced CP model in pigs. The study found that repetitive cerulein injections not only led to CP and induced inflammation in the intestine, but also impaired the intestinal barrier.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Karolina Zygmunt, Agnieszka Otwinowska-Mindur, Katarzyna Piorkowska, Wojciech Witarski
Summary: It is predicted that by 2040, 35% of meat requirements will be provided through in vitro production. This study focused on developing a medium composition that effectively stimulates the proliferation of bovine satellite cells (BSCs) for cultured meat production. The results indicated that bovine serum and the addition of 10 ng/mL bFGF significantly enhanced the proliferation rate of BSCs, while low glucose content promoted the expression of crucial genes involved in satellite cell self-renewal.
Article
Agriculture, Dairy & Animal Science
Katarzyna Piorkowska, Julia Sroka, Kacper Zukowski, Karolina Zygmunt, Katarzyna Ropka-Molik, Miroslaw Tyra
Summary: This study evaluated the effects of BSCL2 variants on slaughter and fattening characteristics and meat quality traits. The selection of mutations was based on variant calling analysis and chi test results using subcutaneous fat RNA-seq data. Significant genotype/allele distribution variations between high- and low-fat pigs were observed. The study suggests that variant calling from RNA-seq data may help develop genetic markers for complex pig traits, and the BSCL2 G allele (rs341493267) was found to increase backfat thickness in pigs.
Review
Genetics & Heredity
Monika Stefaniuk-Szmukier, Katarzyna Piorkowska, Katarzyna Ropka-Molik
Summary: Equine metabolic syndrome (EMS) is an important issue in modern veterinary medicine, linked to the painful hoof laminitis. We have summarized available information on the genetic background, environmental factors, diagnosis, treatment, and management of EMS for both scientific and practical purposes. Clinical presentation in horses includes obesity, lameness, and insulin dysregulation, with ongoing research in regenerative medicine for promising therapies.
Article
Agriculture, Dairy & Animal Science
Angelika Podbielska, Katarzyna Piorkowska
Summary: Genotyping-by-sequencing (GBS) is a useful technique for discovering high-quality SNPs and identifying hybrid individuals between different species. GBS can also be applied to finding backcrosses and has unlimited potential for use with restriction enzymes and species without reference genomes.
ANNALS OF ANIMAL SCIENCE
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
Kuba Chrobocinski, Wojciech Witarski, Katarzyna Piorkowska
Summary: This paper presents an algorithm for automated count and outline of hepatocytes using filters and texture analysis. The algorithm includes a series of filters and algorithms for morphological segmentation, and there are plans for further improvement and development.
INTELLIGENT COMPUTING, VOL 3
(2022)
Article
Agriculture, Dairy & Animal Science
Katarzyna Piorkowska, Kacper Zukowski, Katarzyna Ropka-Molik, Miroslaw Tyra
Summary: This study used pigs as an animal model to investigate the potential gene expression regulation by lncRNAs in fat tissue. The results showed that the expression of hundreds of genes, including 8 lncRNAs, was dependent on fat content. It was also discovered that a transcript related to human insulin resistance was identified, and some lncRNAs may play a regulatory role in gene expression.
ANNALS OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Magdalena Szyndler-Nedza, Aurelia Mucha, Katarzyna Ropka-Molik, Katarzyna Piorkowska
Summary: This study aimed to search for new polymorphisms in genes related to lactogenesis and determine their relationship with colostrum and milk composition and piglets' body weight. The study found that polymorphisms in the B4GALT1 gene had a significant effect on colostrum and milk composition and piglets' body weight changes.
ANNALS OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Adrian Lopez-Catalina, Valentin Costes, Ramon Peiro-Pastor, Helene Kiefer, Oscar Gonzalez-Recio
Summary: Epigenetic marks can explain part of the phenotypic variance in animal breeding models. Nanopore sequencing shows high correlation with bisulphite sequencing in identifying DNA methylation marks. Different sequencing methods highlight differentially methylated genes and positions of economic interest in animal breeding.
