Article
Veterinary Sciences
Cristina Cuello, Cristina A. Martinez, Josep M. Cambra, Alejandro Gonzalez-Plaza, Inmaculada Parrilla, Heriberto Rodriguez-Martinez, Maria A. Gil, Emilio A. Martinez
Summary: The study showed that vitrification altered the gene expression pattern of porcine morulae produced in vivo, leading to disruptions in metabolic pathways that may interfere with embryo quality and developmental potential.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Chenxi Wang, Yang Yang, Nan Gao, Jing Lan, Xiujing Dou, Jianping Li, Anshan Shan
Summary: The study demonstrates that l-threonine can effectively increase the expression of beta-defensins and reduce inflammatory cytokines in porcine intestinal epithelial cells. Furthermore, l-threonine helps alleviate LPS-induced intestinal mucosal barrier damage, suggesting its potential as a promising approach to enhance disease resistance and intestinal health in animals.
Article
Veterinary Sciences
Jiayun Wu, Xiaoru Shi, Lisi Wu, Zhengchang Wu, Shenglong Wu, Wenbin Bao
Summary: This study conducted a whole genome analysis of DNA methylation and expression in TGEV-infected porcine testicular cells (ST cells) and identified differentially expressed genes (DEGs) enriched in viral defense and ribosome biogenesis pathways. Two crucial genes were found to be negatively correlated to promoter methylation. This provides insights into the functional interactions in TGEV infection.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Guoqi Su, Yuheng Luo, Daiwen Chen, Bing Yu, Jun He
Summary: The study identified porcine beta-defensin 114 (PBD114) as an endotoxin-responsive gene expressed in intestinal epithelial cells (IECs) and found that it regulates NF-κB-dependent inflammation in response to various infections or diseases, maintaining intestinal homeostasis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Dairy & Animal Science
Ales Knoll, Michaela Nesvadbova, Tomas Urban
Summary: NREP gene plays a critical role in muscle transformation in pigs, with higher expression levels in Czech Large White pigs compared to wild boars and positive associations with meat-related traits, suggesting its potential as a marker for selection in improving pork production.
JOURNAL OF ANIMAL BREEDING AND GENETICS
(2022)
Article
Genetics & Heredity
Xiaolong Tian, Jun Ma, Yijing Wu, Pan Zhang, Qinggang Li, Hao Zhang, Changxin Wu
Summary: High-altitude adaptation in Tibetan pigs is associated with positively-selected genes, with the BNP gene playing a critical role. The C-1780G polymorphic site in the BNP gene is advantageous for high-altitude adaptation in pigs by upregulating BNP expression. This study provides insights into the mechanism underlying the involvement of the BNP gene in high-altitude adaptation.
Article
Biochemistry & Molecular Biology
Kun Zhang, Shaoqiang Lian, Xiaoyang Shen, Xinhao Zhao, Weidong Zhao, Chunli Li
Summary: This study explores the immunomodulatory activity and mechanisms of recombinant pBD2 against Escherichia coli infection. The results show that pBD2 can reduce the adhesion of E. coli to cells and restore the abnormal expression of ZO-1 and occludin. Additionally, pBD2 can also reduce cell apoptosis and inflammatory responses, acting through the TAK1-NF-κB signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Damian Tanski, Agnieszka Skowronska, Malgorzata Tanska, Ewa Lepiarczyk, Mariusz T. Skowronski
Summary: The research shows that estradiol, progesterone, and arachidonic acid regulate the expression of AQP1 and AQP5 in porcine luminal epithelial cells through the PKA and MAPK signaling pathways.
Article
Environmental Sciences
Qinghao Li, Man Zhang, Juan Sun, Yilei Li, Shaopo Zu, Yuqiang Xiang, Xin Jin
Summary: This study investigated the molecular mechanisms of pBD-2 in alleviating AFB1-induced oxidative stress and intestinal mucosal damage. The findings revealed that pBD-2 reduced ROS production, decreased inflammatory response, and restored intestinal mucosal barrier function.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Yang Yu, Xuechun Li, Yimei Li, Renyue Wei, Hai Li, Zhonghua Liu, Yu Zhang
Summary: This study establishes a stable iPSC-EC induction system and investigates the regulatory mechanism of endothelial differentiation. The derived piPSC-ECs form microvessel-like structures in vitro and promote blood vessel formation under pathological conditions. The activation of TGF-beta-Smad2/3 signaling promotes mesoderm formation and prevents endothelial differentiation in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Fanwen Zeng, Mingming Wang, Ju Li, Chengde Li, Xueqing Pan, Li Meng, Li Li, Hengxi Wei, Shouquan Zhang
Summary: In this study, we found that porcine beta-defensin 129 (pBD129) is widely present in accessory sex glands and coats the surface of sperm. Inhibiting pBD129 on the sperm surface resulted in increased acrosome integrity, tyrosine phosphorylation levels, and Ca2+ influx, leading to a reduced in vitro fertilization cleavage rate. Furthermore, treatment with recombinant pBD129 restored sperm motility in semen contaminated with Escherichia coli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kunhong Xie, Guoqi Su, Daiwen Chen, Bing Yu, Zhiqing Huang, Jie Yu, Ping Zheng, Yuheng Luo, Hui Yan, Hua Li, Jun He
Summary: The study showed that pBD129 is highly expressed in Tibetan pigs compared to DLY pigs and is significantly upregulated upon E. coli infection. The pBD129 protein has antibacterial activity, is non-toxic to cells, and reduces apoptosis of porcine intestinal epithelial cells exposed to bacterial endotoxins. Additionally, it modulates inflammatory responses by down-regulating inflammatory cytokines and apoptosis-related genes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Cell Biology
Hanjun Zhao, Kelvin Kai-Wang To, Hoiyan Lam, Chuyuan Zhang, Zheng Peng, Xinjie Meng, Xiankun Wang, Anna Jinxia Zhang, Bingpeng Yan, Jianpiao Cai, Man Lung Yeung, Jasper Fuk-Woo Chan, Kwok-Yung Yuen
Summary: The human-derived peptide 4H30 demonstrates broad antiviral activity against SARS-CoV-2 and its variants, inhibiting viral entry, fusion, and release.
Article
Microbiology
Peng Shang, Mingbang Wei, Mengqi Duan, Feifei Yan, Yangzom Chamba
Summary: This study found that the composition and abundance of colonic intestinal microflora in Tibetan pigs were closely related to their superior traits. Bifidobacteria, Ruminococcaceae, and Family-XIII-AD3011-Group are conducive to improving disease resistance in Tibetan pigs, while Lactobacillus and Solobacterium are the main bacterial communities involved in fat deposition in Tibetan pigs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Pengju Zhao, Xianrui Zheng, Ying Yu, Zhuocheng Hou, Chenguang Diao, Haifei Wang, Huimin Kang, Chao Ning, Junhui Li, Wen Feng, Wen Wang, George E. Liu, Bugao Li, Jacqueline Smith, Yangzom Chamba, Jian-Feng Liu
Summary: This study identified a large number of unknown protein isoforms from normal pig tissues and systematically characterized them, enhancing the study of the pig proteome. Through high-throughput RNA sequencing, the functional annotations of these newly identified protein isoforms were analyzed, providing a basis for further improving genome annotation. Finally, a significant number of orthologous genes for unknown protein isoforms across multiple species were mined, referencing various pathways and providing valuable resources for pig genomics and biology research.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Agriculture, Dairy & Animal Science
Adrian Lopez-Catalina, Valentin Costes, Ramon Peiro-Pastor, Helene Kiefer, Oscar Gonzalez-Recio
Summary: Epigenetic marks can explain part of the phenotypic variance in animal breeding models. Nanopore sequencing shows high correlation with bisulphite sequencing in identifying DNA methylation marks. Different sequencing methods highlight differentially methylated genes and positions of economic interest in animal breeding.
