Article
Neurosciences
Eduard Bentea, Laura De Pauw, Lise Verbruggen, Lila C. Winfrey, Lauren Deneyer, Cynthia Moore, Giulia Albertini, Hideyo Sato, Ann Van Eeckhaut, Charles K. Meshul, Ann Massie
Summary: Our study revealed age-related protection against proteasome inhibition-induced nigrostriatal degeneration in xCT(-/-) mice, while xCT deletion failed to protect nigral dopaminergic neurons of aged mice against MPTP-induced toxicity. This provides new insights into the role of system x(c)(-) in mechanisms of dopaminergic cell loss and its interaction with aging.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Lei Wang, Xinghong Wu, Ge Yang, Nan Hu, Zijian Zhao, Lei Zhao, Shengyu Li
Summary: This study investigated the therapeutic effect of cannabidiol (CBD) on Parkinson's disease (PD) mice. The results showed that CBD improved cognitive dysfunction and locomotion in PD mice by inhibiting neuroinflammation and dopaminergic neuron apoptosis.
Article
Neurosciences
Zijuan Zhang, Hai Li, Yunfang Su, Jinlian Ma, Ye Yuan, Ziyang Yu, Ming Shi, Simai Shao, Zhenqiang Zhang, Christian Holscher
Summary: Research demonstrates that the CCK analogue has neuroprotective effects and improves locomotor function in an acute PD mouse model. It acts by restoring dopaminergic neuron count and synapse number, reducing neuroinflammation and autophagy impairment, and protecting against mitochondrial and ER damage.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Neurosciences
Tsu-Kung Lin, Kai-Jung Lin, Hung-Yu Lin, Kai-Lieh Lin, Min-Yu Lan, Pei-Wen Wang, Tzu-Jou Wang, Feng-Sheng Wang, Po-Chin Tsai, Chia-Wei Liou, Jiin-Haur Chuang
Summary: Parkinson's disease is a common neurodegenerative disorder with no known treatments to slow its progression. Recent studies suggest that anti-diabetic drugs like GLP-1RA may have neuroprotective effects in PD by improving mitochondrial morphology balance and enhancing damaged organelle degradation.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Immunology
Hyongjun Jeon, Chang-Hwan Bae, Yukyoung Lee, Hee-Young Kim, Seungtae Kim
Summary: Korean red ginseng (KRG) has anti-inflammatory effects in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease mouse model, and can influence microbiome composition, thereby preventing dopaminergic neuronal death and expression of inflammatory factors.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Chemistry, Applied
Vincent M. Lombardo, Louise Bernier, Ming Z. Chen, William Farrell, Andrew Flick, Philippe Nuhant, Neal W. Sach, Yong Tao, John Trujillo
Summary: Chiral piperidines are commonly found in approved drugs and drug candidates, but their synthesis faces challenges of scalability and stereochemical control. This research focused on accessing stereochemically pure 3,4-disubstituted piperidines through a classical resolution method, which could have broader utility in pharmaceutical intermediates. The practical synthesis described in this work allows for the production of kilogram quantities of enantiopure precursor, enabling scale-up efforts for toxicology studies.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Pharmacology & Pharmacy
Benchi Cai, Lifan Zhong, Qitong Wang, Wendi Xu, Xi Li, Tao Chen
Summary: This study investigates the neuroprotective mechanisms of curcumin for Parkinson's disease through the microbiota-gut-brain axis, and finds that curcumin alleviates motor deficits and gut dysfunctions by regulating the gut microbiota and short-chain fatty acids.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Behavioral Sciences
Abdolvahed Narmashiri, Mojtaba Abbaszadeh, Ali Ghazizadeh
Summary: This article provides a comprehensive review and meta-analysis on the effects of MPTP on memory and motor functions in Parkinson's disease. The results show that MPTP not only significantly affects motor functions, but also various mnemonic processes. However, no systematic relationship between the MPTP injection protocol parameters and the extent of induced PD symptoms was found.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2022)
Article
Oncology
Jing Zhang, Bohao Sun, Jifeng Yang, Zhuo Chen, Zhengzheng Li, Nan Zhang, Hongzhi Li, Luxi Shen
Summary: This study compared the effects of rotenone and MPTP in two commonly used mouse models of chronic PD. The results showed that the MPTP model accurately reproduced the pathological features of PD, while the rotenone model was more suitable for studying mitochondrial dysfunction and Lewy body formation in PD.
MOLECULAR MEDICINE REPORTS
(2022)
Article
Crystallography
Maya Chochkova, Rusi Rusew, Reni Kalfin, Lyubka Tancheva, Maria Lazarova, Hristina Sbirkova-Dimitrova, Andrey Popatanasov, Krasimira Tasheva, Boris Shivachev, Nejc Petek, Martin Sticha
Summary: Parkinson's disease is a common neurodegenerative disorder with severe side effects associated with current treatments. In this study, a new compound, CA(2-Me)-Am, was synthesized and shown to improve memory performance and neuromuscular coordination in an experimental model of Parkinson's disease.
Article
Pharmacology & Pharmacy
Babita Singh, Shivani Pandey, Mohammad Rumman, Shashank Kumar, Prem Prakash Kushwaha, Rajesh Verma, Abbas Ali Mahdi
Summary: The study showed that Bacopa monnieri extract has neuroprotective and neurorescue effects against MPTP-induced degeneration of dopaminergic neurons, potentially slowing down disease progression and delaying neurodegeneration in Parkinson's disease.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Neurosciences
Adeola Oluwatosin Adedara, Guilherme Wildner, Julia Sepel Loreto, Matheus Mulling dos Santos, Amos Olalekan Abolaji, Nilda Vargas Barbosa
Summary: The study investigated the protective effects of kaempferol (KMP) against the toxicity induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in Drosophila melanogaster. It was found that MPTP exposure caused mortality, developmental disorders, locomotor deficits, disruption of circadian rhythm, oxidative stress, and mitochondrial dysfunction in flies. However, KMP supplementation counteracted these toxic effects, improving survival, locomotion, circadian cycle, and restoring antioxidant enzymes and mitochondrial functionality. Therefore, KMP shows promise in mitigating the toxic effects of MPTP and potentially in the treatment of Parkinson's disease.
Article
Behavioral Sciences
Yongjun Wang, Zhengjia Pu, Yiran Zhang, Zhaohui Du, Zeming Guo, Qunhua Bai
Summary: Parkinson's disease (PD) is a common neurodegenerative disease whose exact cause is unclear, but gut microbial disorders are believed to play a role in its development. This study investigated the effects of exercise training on PD by subjecting a chronic mouse model of the disease to rotarod walking training. The results showed that exercise training improved motor function, increased dopamine content, and promoted the gene expression of important factors in the striatum, indicating potential protection against MPTP-induced damage. Additionally, exercise training promoted recovery from ileal pathology, reduced intestinal inflammation, and significantly altered the composition and structure of the intestinal flora.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Fumet Duygu Ustundag, Ismail Unal, Derya Cansiz, Unsal Veli Ustundag, Hulya Kara Subasat, A. Ata Alturfan, Pinar Mega Tiber, Ebru Emekli-Alturfan
Summary: This study investigated the effects of 3-pyridinylboronic acid on MPTP exposed zebrafish embryos. The results showed that 3-pyridinylboronic acid restored locomotor activity and gene expressions related to mitochondrial dysfunction and oxidative stress caused by MPTP.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Article
Nutrition & Dietetics
Zhili Ren, Hui Ding, Ming Zhou, Piu Chan
Summary: In this study, the neuroprotective effects of Ganoderma lucidum extract (GLE) in a Parkinson's disease (PD) model were explored. GLE was found to inhibit microglia activation and regulate abnormal cytokine expression levels. It also suppressed iNOS/NLRP3 pathway and abnormal microglial autophagy and lysosomal degradation, providing new evidence for the treatment of PD using Ganoderma lucidum.
Review
Clinical Neurology
Daisuke Ito, Satoru Morimoto, Shinichi Takahashi, Kensuke Okada, Jin Nakahara, Hideyuki Okano
Summary: Significant progress has been made in drug screening for amyotrophic lateral sclerosis using patient-derived induced pluripotent stem cells, leading to the identification of candidate drugs and the advancement of clinical trials. This stem cell-based approach shows safety and positive effects, reducing costs and time compared to animal testing.
Article
Neurosciences
Kanako Muta, Junichi Hata, Naoki Kawaguchi, Yawara Haga, Daisuke Yoshimaru, Kei Hagiya, Takaaki Kaneko, Takako Miyabe-Nishiwaki, Yuji Komaki, Fumiko Seki, Hirotaka James Okano, Hideyuki Okano
Summary: This study evaluated the effects of different sedatives and anesthetics on the resting brain function in common marmosets and found that midazolam and dexmedetomidine had similar sedative effects, while propofol preserved the resting brain function better. The combination of dexmedetomidine and isoflurane showed intermediate effects.
Article
Dermatology
Munenari Itoh, Shiho Kawagoe, Hidemi Nakagawa, Akihiko Asahina, Hirotaka James Okano
Summary: This study utilized induced pluripotent stem cells (iPSCs) as an unlimited cell source to re-differentiate specific T cells into cytotoxic T cells, providing a new solution for immunotherapy of melanoma.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ryota Tamura, Hiroyuki Miyoshi, Kent Imaizumi, Masahiro Yo, Yoshitaka Kase, Tsukika Sato, Mizuto Sato, Yukina Morimoto, Oltea Sampetrean, Jun Kohyama, Munehisa Shinozaki, Atsushi Miyawaki, Kazunari Yoshida, Hideyuki Saya, Hideyuki Okano, Masahiro Toda
Summary: This study demonstrates the potential benefit of gene-edited induced pluripotent stem cells (iPSCs) for the treatment of invasive glioma stem cells (GSCs). Neural stem cells (NSCs) derived from CRISPR/Cas9-edited iPSCs expressing a suicide gene showed enhanced tumor-trophic migratory capacity and significant in vivo antitumor effects. The study also identified key signaling pathways and gene expressions associated with the efficacy of therapeutic iPSC-NSCs.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Satoru Morimoto, Koichi Saeki, Masaru Takeshita, Kunio Hirano, Mariko Shirakawa, Yumiko Yamada, Shiho Nakamura, Fumiko Ozawa, Hideyuki Okano
Summary: The study developed an intranasal vaccine for COVID-19 by using a Sendai virus vector loaded with the receptor binding domain of SARS-CoV-2 as an antigen. The vaccine induced a strong immune response, with increased levels of S-RBD-specific antibodies in serum and bronchoalveolar lavage fluid. The booster dose further enhanced the production of neutralizing antibodies, indicating the effectiveness of the vaccine.
Article
Cell Biology
Sho Yoshimatsu, Mayutaka Nakajima, Iki Sonn, Rie Natsume, Kenji Sakimura, Ena Nakatsukasa, Toshikuni Sasaoka, Mari Nakamura, Takashi Serizawa, Tsukika Sato, Erika Sasaki, Hongkui Deng, Hideyuki Okano
Summary: This study compared the gene expression profiles of mouse, human, and common marmoset EPSCs using transcriptomic approaches. The marmoset EPSC-like cells displayed a distinct morphology but exhibited a pluripotent state similar to embryonic stem cells. They also showed interspecies chimeric contribution to mouse embryos. Furthermore, the gene expression of the marmoset EPSC-like cells resembled that of human EPSCs. Overall, this study evaluated the efficacy of the method for deriving marmoset EPSCs.
Article
Biochemistry & Molecular Biology
Tosho Kondo, Ihori Ebinuma, Hirotaka Tanaka, Yukitoshi Nishikawa, Takaki Komiya, Mitsuru Ishikawa, Hideyuki Okano
Summary: In this study, an effective high-throughput screening system was established using induced pluripotent stem cells (iPSCs) to generate motor neurons. The generated motor neurons exhibited characteristics similar to ALS neurons and showed abnormal protein accumulation and hyperexcitability. Treatment with rapamycin and retigabine normalized activity and improved neuronal survival. This study provides a valuable platform for discovering novel ALS therapeutics and personalized medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Junichi Hata, Ken Nakae, Hiromichi Tsukada, Alexander Woodward, Yawara Haga, Mayu Iida, Akiko Uematsu, Fumiko Seki, Noritaka Ichinohe, Rui Gong, Takaaki Kaneko, Daisuke Yoshimaru, Akiya Watakabe, Hiroshi Abe, Toshiki Tani, Hiro Taiyo Hamda, Carlos Enrique Gutierrez, Henrik Skibbe, Masahide Maeda, Frederic Papazian, Kei Hagiya, Noriyuki Kishi, Shin Ishii, Kenji Doya, Tomomi Shimogori, Tetsuo Yamamori, Keiji Tanaka, Hirotaka James Okano, Hideyuki Okano
Summary: Magnetic resonance imaging (MRI) is a non-invasive neuroimaging technique used for studying normal development and aging, and for sharing data. The marmoset model is effective in aging research due to its shorter life expectancy and faster growth and aging rate. This study investigated the aging process of the marmoset brain and provided a comprehensive MRI database, making it the largest public dataset in the world. The database includes multi-contrast MRI images and can contribute to the understanding of various factors' effects on the brain and accelerate global brain science studies.
Article
Biochemistry & Molecular Biology
Hitomi Ono-Minagi, Tsutomu Nohno, Takashi Serizawa, Yu Usami, Takayoshi Sakai, Hideyuki Okano, Hideyo Ohuchi
Summary: This study investigates the embryonic origin of salivary and lacrimal glands using transgenic mice and demonstrates that these glands contain cells derived from all three germ layers. Specifically, a subset of cells derived from neural crest differentiate into epithelial cells and express high levels of stem cell markers. These cells play an important role in the regenerative response following tissue damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Keigo Hikishima, Tomokazu Tsurugizawa, Kazumi Kasahara, Ryusuke Hayashi, Ryo Takagi, Kiyoshi Yoshinaka, Naotaka Nitta
Summary: The loud acoustic noise from the scanner during fMRI can affect the resting state functional connectivity (FC). In this study, the effects of different acoustic noise levels on FC were investigated using fUS, a neuroimaging method without audible acoustic noise, and compared to the results of rsfMRI. The findings suggest that the acoustic noise level should be considered when evaluating the anticorrelation between brain networks using rsfMRI.
Article
Neurosciences
Keigo Hikishima, Tomokazu Tsurugizawa, Kazumi Kasahara, Ryo Takagi, Kiyoshi Yoshinaka, Naotaka Nitta
Summary: Functional ultrasound (fUS) imaging provides a method for visualizing deep brain activity by measuring cerebral blood volume changes coupled with neural activity, while functional MRI (fMRI) relies on the blood-oxygenation-level-dependent signal. Resting-state functional MRI (rsfMRI) allows for whole brain functional imaging and estimation of resting-state networks (RSNs). Recent research suggests that fUS can provide similar RSNs to fMRI but with higher temporal resolution and stronger correlation with neural activity. These findings have the potential to advance our understanding of functional brain networks and their relationships with disease models.
Article
Clinical Neurology
Hiroya Kobayashi, Koji Ueda, Satoru Morimoto, Mitsuru Ishikawa, Nicolas Leventoux, Ryogen Sasaki, Yoshifumi Hirokawa, Yasumasa Kokubo, Hideyuki Okano
Summary: The proteins in extracellular vesicles derived from astrocytes in patients with Kii ALS/PDC exhibit distinctive patterns compared to healthy individuals, with increased proteins related to proteostasis and decreased proteins related to anti-inflammation.
NEUROLOGICAL SCIENCES
(2023)
Review
Medicine, General & Internal
Takahiro Shibata, Syoichi Tashiro, Masaya Nakamura, Hideyuki Okano, Narihito Nagoshi
Summary: Cell transplantation therapy using human-induced pluripotent stem cell-derived neural stem/progenitor cells has shown efficacy in treating the subacute phase of spinal cord injury. However, there is a lack of focus on treatment in the chronic phase, which poses challenges in overcoming factors specific to chronic SCI. Combining different therapies and addressing issues in severe injury models and functional recovery are important for regenerative therapy in chronic SCI.
MEDICINA-LITHUANIA
(2023)
Article
Geriatrics & Gerontology
Shohei Komaki, Masatoshi Nagata, Eri Arai, Ryo Otomo, Kanako Ono, Yukiko Abe, Hideki Ohmomo, So Umekage, Natsuko Shinozaki, Tsuyoshi Hachiya, Yoichi Sutoh, Yayoi Otsuka-Yamasaki, Yasumichi Arai, Nobuyoshi Hirose, Akio Yoneyama, Hideyuki Okano, Makoto Sasaki, Yae Kanai, Atsushi Shimizu
Summary: This study explored the epigenetic signatures of exceptional healthy longevity and found that it depends not only on maintaining young epigenetic states but also on advanced states of specific epigenetic regions.
LANCET HEALTHY LONGEVITY
(2023)
Review
Biochemistry & Molecular Biology
Hideyuki Okano, Satoru Morimoto, Chris Kato, Jin Nakahara, Shinichi Takahashi
Summary: In recent years, clinical trials using iPSC technology for disease modeling and drug discovery have shown promising results, particularly for the treatment of ALS. ROPi has been identified as a potential ALS treatment and has undergone clinical trials and reverse translational research, demonstrating its ability to delay disease progression and prolong survival in ALS patients.
JOURNAL OF NEUROCHEMISTRY
(2023)