Article
Biochemistry & Molecular Biology
JuDong Yeo, Christopher C. Parrish
Summary: Shotgun lipidomics was used to identify and quantify phospholipids in salmon muscle tissue, focusing on omega-3 fatty acids distribution and eicosanoids. 43 PL species were identified, with 16:0-22:6 PtdCho being predominant. Additionally, prostaglandin E2 and prostaglandin F3 alpha were identified for the first time in salmon muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Lin Ding, Jinyue Yang, Haoran Guo, Peixu Cong, Jie Xu, Changhu Xue, Xiangzhao Mao, Tiantian Zhang, Yuming Wang
Summary: Dietary supplementation of docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) in the form of phosphoethanolamine plasmalogens (PlsEtn) has been shown to remodel lipidome and regulate fatty acid metabolic process in white adipose tissue (WAT), suggesting it as a potential strategy for obesity treatment in the future.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
John Quinones, Rommy Diaz, Jorge F. Beltran, Lidiana Velazquez, David Cancino, Erwin Munoz, Patricio Dantagnan, Adrian Hernandez, Nestor Sepulveda, Jorge G. Farias
Summary: This study analyzed the effects of different feeds on the lipid composition of juvenile Oncorhynchus mykiss muscle and found that the inclusion of Brassica napus oil significantly increased certain lipid levels, while Durvillaea antarctica meal had a less significant impact on lipid variation.
Article
Plant Sciences
Felisa Rey, Paulo Cartaxana, Sonia Cruz, Tania Melo, M. Rosario Domingues
Summary: Marine algae are important sources of high-value compounds. This study focused on the lipidome and pigment profile of Acetabularia acetabulum, a macroscopic green single-celled alga. Analysis identified 16 polar lipid classes and 191 lipid species, as well as carotenoids and chlorophylls. The lipid extracts showed high antioxidant activity.
JOURNAL OF PHYCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Katharina Lackner, Sabrina Sailer, Jan-Bert van Klinken, Eric Wever, Mia L. Pras-Raves, Adrie D. Dane, Masanori Honsho, Yuichi Abe, Markus A. Keller, Georg Golderer, Gabriele Werner-Felmayer, Yukio Fujiki, Frederic M. Vaz, Ernst R. Werner, Katrin Watschinger
Summary: AGMO degrades plasmanyl lipids, while PEDS1 synthesizes plasmalogens. Plasmalogens are associated with neurological diseases. AGMO-deficient mice show moderate accumulation of plasmanyl and plasmenyl lipids, while Peds1 knockout mice have reduced plasmenyl lipids but increased plasmanyl lipids. The rate-limiting enzyme FAR1 is not upregulated in Peds1-deficient mice.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Biochemistry & Molecular Biology
Dmitry Senko, Anna Gorovaya, Elena Stekolshchikova, Nickolay Anikanov, Artur Fedianin, Maxim Baltin, Olga Efimova, Daria Petrova, Tatyana Baltina, Mikhail A. Lebedev, Philipp Khaitovich, Anna Tkachev
Summary: The study assessed blood lipidome alterations in a rat sciatic nerve crush injury model and found delayed systematic alterations in fatty acid metabolism after nerve injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Michaela Chocholouskova, Denise Wolrab, Robert Jirasko, Hana Studentova, Bohuslav Melichar, Michal Holcapek
Summary: This study systematically investigates the effects of different lipid class separation methods and mass spectrometers on quantitative lipidomic data, finding slight variations in molar concentrations even within the same laboratory using different platforms. Normalization to a reference sample can significantly reduce these differences, leading to highly homogenous molar concentrations of individual lipid species. This strategy suggests a potential approach for reporting comparable quantitative results regardless of the analytical platform and mass spectrometer used.
Article
Biochemical Research Methods
Dorota Garwolinska, Michal Mlynarczyk, Agata Kot-Wasik, Weronika Hewelt-Belka
Summary: The study found that storage of human milk samples at 4 degrees Celsius for 4 days and subjecting them to three freeze-thaw cycles can lead to changes in lipid content. Differences in levels of certain lipid species between samples stored at -20 degrees Celsius and -80 degrees Celsius were not statistically significant, and individual variance remained consistent regardless of sample storage conditions. Storing HM samples at -20 degrees Celsius for up to 3 weeks and at -80 degrees Celsius for up to 12 weeks ensures sample lipidome stability.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Harald C. Koefeler, Robert Ahrends, Erin S. Baker, Kim Ekroos, Xianlin Han, Nils Hoffmann, Michal Holcapek, Markus R. Wenk, Gerhard Liebisch
Summary: Lipidomics has rapidly grown in the past 20 years, leading to the need for guidelines to ensure high standards of data quality. The Lipidomics Standards Initiative aims to coordinate the development of best practice guidelines and shed light on the strengths, drawbacks, and pitfalls in the workflow of lipidomic analysis. This review does not provide step-by-step protocols but offers links and original publications for a comprehensive overview of state-of-the-art practices in the field.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jean-Baptiste Domergue, Cinzia Bocca, Rosine De Paepe, Guy Lenaers, Anis M. Limami, Guillaume Tcherkez
Summary: Disruption of mitochondrial complex I (CI) has been found to cause multiple changes in cellular lipids, including leaf, pollen, and seed lipids. This suggests that mitochondrial homeostasis plays a crucial role in regulating the whole cellular lipidome through specific signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Sarah Kupsch, Lars F. Eggers, Dietmar Spengler, Nicolas Gisch, Torsten Goldmann, Heinz Fehrenbach, Guido Stichtenoth, Martin F. Krause, Dominik Schwudke, Andra B. Schromm
Summary: A strong inflammatory immune response is the main cause of lung pathology in nARDS. Anionic phospholipids such as POPG, DOPG, and PIP2 have been found to inhibit the activation of inflammasomes. By enriching surfactant with these phospholipids, the anti-inflammatory properties of surfactant can be enhanced.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tao Wang, Tiana F. Koukoulis, Laura J. Vella, Huaqi Su, Adityas Purnianto, Shuai Nie, Ching-Seng Ang, Guangxu Ma, Pasi K. Korhonen, Aya C. Taki, Nicholas A. Williamson, Gavin E. Reid, Robin B. Gasser
Summary: Many parasitic worms have a harmful impact on human and animal populations globally. Extracellular vesicles (EVs) have been found to play a role in modulating inflammatory/immune host responses and parasitism. In this study, EVs from the Haemonchus contortus parasite were enriched and a comprehensive analysis of EV proteins and lipids was conducted. The findings provide insights into the role of EV-specific molecules in parasite-host cross-talk and offer potential strategies to disrupt or eliminate parasite infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Maria Kadyrov, Luke Whiley, Belinda Brown, Kirk Erickson, Elaine Holmes
Summary: One of the notable effects of aging is a decline in brain health and cognitive function, which is linked to disruptions in lipid homeostasis. While aging is the primary risk factor for neurodegenerative diseases like dementia, cognitive decline is commonly observed in adults over 65. Unfortunately, there is a lack of effective methods to improve brain health in old age. However, exercise has shown promise in positively influencing cognitive health and associated lipid profiles.
Article
Nutrition & Dietetics
Carolina Gonzalez-Riano, Marcelo Santos, Marta Diaz, Cristina Garcia-Beltran, Carles Lerin, Coral Barbas, Lourdes Ibanez, David Sanchez-Infantes
Summary: Being born small or large for gestational age, combined with suboptimal early postnatal outcomes, can lead to future metabolic alterations. This pilot study investigated the relationships between birth weight, early postnatal outcomes, and cord blood serum lipidomes. The study found distinct cord blood lipidome patterns in different birth weight groups, which were associated with parameters related to growth and glucose homeostasis. These findings suggest that the lipidome patterns may play a role in adipose tissue remodeling and metabolic risks in the future.
Article
Biochemistry & Molecular Biology
Martina Zandl-Lang, Thomas Zuellig, Martin Troetzmueller, Yvonne Naegelin, Lucia Abela, Bernd Wilken, Sabine Scholl-Buergi, Daniela Karall, Ludwig Kappos, Harald Koefeler, Barbara Plecko
Summary: Rett syndrome (RTT) is a rare disease caused by mutations of the methyl-CpG binding protein 2 (MECP2) gene, and it is one of the most common causes of genetic mental retardation in girls. This study found altered CSF lipidomics profile in RTT patients, providing a basis for future studies on selected lipid species as CSF biomarkers for RTT.
Article
Biochemistry & Molecular Biology
Karel Horejsi, Robert Jirasko, Michaela Chocholouskova, Denise Wolrab, David Kahoun, Michal Holcapek
Summary: In this study, GSL subclasses were isolated from human plasma using an optimized ethanol-water solvent system, followed by purification and analysis using HILIC-ESI-LIT-MS/MS. The results provide detailed profiling and characterization of GSL and phospholipids in human plasma, with the identification of 154 GSL species and 77 phospholipids. The developed method has potential applications in clinical and biological research of GSL in human blood and other biological samples.
Article
Biochemical Research Methods
Zuzana Vankova, Ondrej Peterka, Michaela Chocholouskova, Denise Wolrab, Robert Jirasko, Michal Holcapek
Summary: The RP-UHPLC/MS method was developed to identify over 500 lipid species representing 26 polar and nonpolar lipid classes in human plasma. High-resolution mass spectrometry and chromatographic techniques were employed to accurately identify lipid species.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Denise Wolrab, Robert Jirasko, Eva Cifkova, Marcus Horing, Ding Mei, Michaela Chocholouskova, Ondrej Peterka, Jakub Idkowiak, Tereza Hrnciarova, Ladislav Kuchar, Robert Ahrends, Radana Brumarova, David Friedecky, Gabriel Vivo-Truyols, Pavel Skrha, Jan Skrha, Radek Kucera, Bohuslav Melichar, Gerhard Liebisch, Ralph Burkhardt, Markus R. Wenk, Amaury Cazenave-Gassiot, Petr Karasek, Ivo Novotny, Kristina Greplova, Roman Hrstka, Michal Holcapek
Summary: Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve the survival time prognosis of patients. The study found significant differences in blood lipids between pancreatic cancer patients and healthy controls.
NATURE COMMUNICATIONS
(2022)
Article
Toxicology
Jaroslav Pejchal, Petr Jost, Lubica Muckova, Rudolf Andrys, Miroslav Lisa, Jana Zdarova Karasova
Summary: This study focused on the safety and pharmacokinetics of cucurbit[7]uril (CB[7]). The results showed that CB[7] had no cytotoxicity in human renal cells, but non-parenteral administration caused tissue damage to the kidneys.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Oleksandr Kozlov, Miroslav Lisa
Summary: Powerful chromatographic techniques, such as ultrahigh-performance supercritical fluid chromatography (UHPSFC), have been proven to be effective for lipidomic analysis. This study focused on the intraclass separation of lipids using UHPSFC, investigating the effects of various chromatographic conditions. The results showed that UHPSFC can achieve both interclass selectivity and intraclass separation of lipids, providing valuable insights for lipid analysis.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
Denise Wolrab, Ondrej Peterka, Michaela Chocholouskova, Michal Holcapek
Summary: Ultrahigh-performance supercritical fluid chromatography/mass spectrometry (UHPSFC/MS) is a fast analytical method for high-throughput lipidomic analysis. It offers lipid class separation and lipid species separation modes, with fast analysis time and comparable reproducibility and sensitivity.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Miroslav Lisa, Tereza Jirankova
Summary: Recent research has shown that ultrahigh-performance supercritical fluid chromatography - mass spectrometry (UHPSFC/MS) is an excellent tool for fast and comprehensive lipid characterization. However, the long-term instability of retention times using UHPSFC needs to be addressed. This study evaluated the interclass separation of lipid extracts using UHPSFC and found that the solvent type used as a modifier has a significant effect on resolution. The optimized UHPSFC/MS method can provide resolution of all lipid classes in brain samples within 7 minutes and has excellent retention time repeatability in lipidomic analysis of biological samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Oncology
Robert Jirasko, Jakub Idkowiak, Denise Wolrab, Ales Kvasnicka, David Friedecky, Krzysztof Polanski, Hana Studentova, Vladimir Student, Bohuslav Melichar, Michal Holcapek
Summary: This study reveals significant changes in concentrations of sulfatides and sphingomyelins in plasma and urine of RCC patients, and similar alterations in lipid profiles of body fluids and tissues. Binary classifiers are built to detect RCC based on plasma and urine lipidome dysregulations, with the ability to distinguish between early-stage RCC and controls. The results demonstrate the potential of lipid screening in biofluids for RCC detection and monitoring in clinical settings.
Article
Biochemistry & Molecular Biology
Karel Horejsi, Chunsheng Jin, Zuzana Vankova, Robert Jirasko, Ondrej Strouhal, Bohuslav Melichar, Susann Teneberg, Michal Holcapek
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a common and deadly type of cancer with a low survival rate. In-depth lipid profiling and screening of potential biomarkers offer a promising approach for early detection of PDAC or other cancers.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Oleksandr Kozlov, Eliska Horakova, Sara Rademacherova, David Malinak, Rudolf Andrys, Eliska Prchalova, Miroslav Lisa
Summary: A fast and selective method using chiral supercritical fluid chromatography-mass spectrometry (SFC-MS) was developed to study lipase selectivity in the hydrolysis of triacylglycerols (TGs). The method involved the synthesis of enantiomerically pure monoacylglycerol (MG) and diacylglycerol (DG) isomers and their separation using a chiral column and neat methanol as a mobile phase modifier. The results revealed different stereoselectivities for substrates in the lipase-catalyzed hydrolysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Shannon J. McKie, Alex S. Nicholson, Emily Smith, Stuart Fawke, Eve R. Caroe, James C. Williamson, Benjamin G. Butt, Denisa Kolarova, Ondrej Peterka, Michal Holcapek, Paul J. Lehner, Stephen C. Graham, Janet E. Deane
Summary: Myelin is a multilayered membrane that tightly wraps neuronal axons, enabling efficient, high-speed signal propagation. This study reveals that altered lipid metabolism affects the abundance of specific plasma membrane proteins, which play important roles in cell adhesion and signaling, and are implicated in neurological diseases. The study also demonstrates the direct interaction between the adhesion molecule neurofascin (NFASC) and the sphingolipid sulfatide, providing insights into the pathogenesis of demyelinating diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
Jeffrey G. McDonald, Christer S. Ejsing, Dominik Kopczynski, Michal Holcapek, Junken Aoki, Makoto Arita, Masanori Arita, Erin S. Baker, Justine Bertrand-Michel, John A. Bowden, Britta Brugger, Shane R. Ellis, Maria Fedorova, William J. Griffiths, Xianlin Han, Jurgen Hartler, Nils Hoffmann, Jeremy P. Koelmel, Harald C. Kofeler, Todd W. Mitchell, Valerie B. O'Donnell, Daisuke Saigusa, Dominik Schwudke, Andrej Shevchenko, Candice Z. Ulmer, Markus R. Wenk, Michael Witting, Denise Wolrab, Yu Xia, Robert Ahrends, Gerhard Liebisch, Kim Ekroos
Summary: The rapid growth of lipidomic data has led to a community-based movement to develop guidelines and minimum requirements for generating, reporting, and publishing such data. The creation of a dynamic checklist summarizing key details of lipidomic analyses using a common language has the potential to harmonize the field by improving traceability and reproducibility.
Review
Biochemistry & Molecular Biology
Harald C. Koefeler, Robert Ahrends, Erin S. Baker, Kim Ekroos, Xianlin Han, Nils Hoffmann, Michal Holcapek, Markus R. Wenk, Gerhard Liebisch
Summary: Lipidomics has rapidly grown in the past 20 years, leading to the need for guidelines to ensure high standards of data quality. The Lipidomics Standards Initiative aims to coordinate the development of best practice guidelines and shed light on the strengths, drawbacks, and pitfalls in the workflow of lipidomic analysis. This review does not provide step-by-step protocols but offers links and original publications for a comprehensive overview of state-of-the-art practices in the field.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Multidisciplinary Sciences
Denise Wolrab, Robert Jirasko, Ondrej Peterka, Jakub Idkowiak, Michaela Chocholouskova, Zuzana Vankova, Karel Horejsi, Ivana Brabcova, David Vrana, Hana Studentova, Bohuslav Melichar, Michal Holcapek
Summary: Early cancer detection is an unmet need in clinical medicine. Lipidomic profiling of human plasma is investigated to distinguish patients with breast, kidney, and prostate cancers from healthy controls, showing promising sensitivity, specificity, and accuracy. Seven specific lipid species were identified as potential biomarker panel for cancer screening, but further verification in prospective studies is needed for clinical utility.
SCIENTIFIC REPORTS
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
K. Ekroos, G. Liebisch, M. Holcapek, R. Ahrends, H. Kofeler, X. Han, M. Wenk
