Article
Green & Sustainable Science & Technology
Wageeporn Maneechote, Benjamas Cheirsilp, Naruepon Liewtrakul, Sirasit Srinuanpan, Wasu Pathom-aree, Neeranuch Phusunti
Summary: Two promising oleaginous microalgae, Scenedesmus sp. SPP and marine Chlorella sp., were grown under different nutrient modes. They showed the best growth under mixotrophic/nitrogen-rich conditions, indicating their ability to metabolize both inorganic (CO2) and organic (glucose) carbon sources. Lipid accumulation with superior biodiesel properties was promoted under mixotrophic/nitrogen-starvation conditions. The optimization of key energy sources, including light intensity, CO2, and glucose concentrations, for mixotrophic cultivation was successfully performed using Response Surface Methodology. Two-stage mixotrophic cultivation enhanced biomass and lipids, as well as improved biodiesel properties. These strategies are important for the development of microalgae-based biofuel and biochemical industries.
Article
Biotechnology & Applied Microbiology
Jia-Xing Guo, Zhi-Gang Zhou, Carol Sze Ki Lin, Zheng Sun
Summary: This study developed a two-stage cultural strategy for high biomass production and rapid arachidonic acid (ArA) accumulation in Myrmecia incisa, demonstrating its potential as a promising feedstock for ArA-rich oil.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Microbiology
Tan Liu, Zhihui Chen, Yunhua Xiao, Mingmin Yuan, Chenkai Zhou, Gang Liu, Jun Fang, Bo Yang
Summary: Nitrogen limitation and starvation can increase the lipid content of microalgal cells but decrease biomass productivity, pigment concentration, and protein content. Three-day nitrogen starvation is a suitable strategy to produce lipid-rich biomass and causes changes in cell morphology. Saturated fatty acids dominate, making microalgae a potential feedstock for biodiesel production.
Article
Multidisciplinary Sciences
Peng Zhang, Yi Xin, Yuehui He, Xianfeng Tang, Chen Shen, Qintao Wang, Nana Lv, Yun Li, Qiang Hu, Jian Xu
Summary: The protein NobZIP77 regulates oil synthesis by sensing blue light, and these findings can be utilized to enhance oil productivity through genetic and process engineering in microalgae.
NATURE COMMUNICATIONS
(2022)
Article
Nutrition & Dietetics
Yue Liu, Yin-Yin Xia, Ting Zhang, Yang Yang, Richard D. Cannon, Toby Mansell, Boris Novakovic, Richard Saffery, Ting-Li Han, Hua Zhang, Philip N. Baker
Summary: This study investigated the relationship between gestational diabetes mellitus (GDM) and desaturase activities throughout pregnancy. The results showed significant changes in fatty acid levels and desaturase activities in GDM patients compared to non-GDM controls. Particularly, delta-5 desaturase and delta-9-18 desaturase activities may play a role in the pathophysiology of GDM, suggesting potential applications for early diagnosis and management.
FRONTIERS IN NUTRITION
(2022)
Review
Nutrition & Dietetics
Melissa Gonzalez-Soto, David M. Mutch
Summary: This review highlights that mRNA expression and activity of desaturase and elongase enzymes are influenced by various dietary components, including macronutrients, micronutrients, and polyphenols. Understanding how these components influence LC-PUFA synthesis will help further our understanding of how dietary patterns impact disease risks, from caloric excesses to micronutrient deficiencies.
ADVANCES IN NUTRITION
(2021)
Article
Biotechnology & Applied Microbiology
Satoshi Yamamoto, Kouki Ito, Masaharu Tokuda, Naoaki Tsutsui
Summary: This study identified the RhNrt2 and RhCpeb genes and characterized their expression in Rhodomonas species. It was found that the expression level of RhNrt2 significantly increased under nitrogen-starved conditions, while the expression level of RhCpeb was significantly suppressed. The regulated expression of these genes may serve as molecular indicators of nitrate status and intracellular Phycoerythrin biosynthesis.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Pin-Yang Tu, Shin-Jie Huang, Venugopal Rajanbabu, Jen-Leih Wu, Jyh-Yih Chen
Summary: D56 in tilapia regulates pathways and genes related to fatty acid metabolism, inflammation, and immune response, enhancing resistance against V. vulnificus infection.
Article
Biotechnology & Applied Microbiology
Bert Coleman, Christof Van Poucke, Bavo De Witte, Valentina Casciaro, Tanja Moerdijk-Poortvliet, Koenraad Muylaert, Johan Robbens
Summary: The flavor of T. chuii can be modified by changing the nitrogen supply in the cultivation medium, resulting in differences in odor intensity and taste profile. The N-deplete biomass had a stronger odor intensity and earthy off-odor, while the N-replete biomass had a stronger taste intensity, umami, and salty taste. These findings highlight the importance of cultivating conditions in shaping the flavor and palatability of microalgae biomass.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Agriculture, Multidisciplinary
Chen Wu, Nan Jiang, Ruhong Wang, Shanshan Jiang, Zhijia Yuan, Xue Luo, Junrui Wu, Haisu Shi, Rina Wu
Summary: By overexpressing Mucor circinelloides FADS12 in G. candidum, the production of linoleic acid (LA) can be increased in Geotrichum-ripened cheeses, thereby converting saturated fatty acids (SFAs) to unsaturated fatty acids (UFAs). Additionally, the introduction of soybean byproduct (okara) further enhances the level of LA.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agricultural Engineering
Ty Shitanaka, Lauren Higa, Abigail E. Bryson, Conor Bertucci, Natalie Vande Pol, Ben Lucker, Samir Kumar Khanal, Gregory Bonito, Zhi-Yan Du
Summary: This study investigates the potential of using the oleaginous fungus Mortierella alpina to bio-flocculate starchless mutants of Chlamydomonas reinhardtii. By adjusting the nitrogen regime, the lipid concentration of triacylglycerides (TAG) reaches 85%. Scanning electron microscopy reveals that cell-wall attachment and extra polymeric substances (EPS) are responsible for algal flocculation. The optimal algal-fungal biomass ratio of 1:1 results in 80-85% flocculation efficiency in 24 hours. Utilizing nutrient-deprived sta6/sta7 mutants and various strains of M. alpina achieves fatty acid profiles similar to C. reinhardtii, including arachidonic acid (ARA).
BIORESOURCE TECHNOLOGY
(2023)
Article
Optics
Fernando Robert Ferrel Ballestas, Mariana Titica, Jack Legrand, Laurent Pilon, Guillaume Cogne
Summary: This paper aims to develop a new method to predict the radiation characteristics of C. reinhardtii during progressive nitrogen starvation, accounting for physiological changes induced by nutritional stress. The radiation characteristics were predicted based on Lorenz-Mie theory and effective spectral optical properties, and the local rate of photon absorption was determined using the two-flux approximation. The developed tools could be used to generate a database of radiation characteristics for modeling growth and carbon reserve accumulation kinetics in microalgae.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2023)
Article
Biochemistry & Molecular Biology
Junhuan Yang, Xiuwen Wang, Hassan Mohamed, Shaoqi Li, Chen Wu, Wenyue Shi, Futing Xue, Sergio Lopez-Garcia, Yuanda Song
Summary: Linolenic acid (LA) is gaining attention for its potential medical role, and the oleaginous fungus Mucor circinelloides has the ability to produce high lipid content and valuable polyunsaturated fatty acids (PUFAs). This study proposes a genetic engineering method to increase LA production in M. circinelloides and provides evidence for the design of metabolic engineering for LA and PUFA production, with potential industrial applications.
Article
Environmental Sciences
Gang Fu, Yue Qi, Junsheng Li, Caiyun Zhao, Jing He, Yiwen Ma, Jinfang Zhu
Summary: This study investigated the spatial distribution of nitrogen and phosphorus in the intertidal flats of the Yellow River Delta, finding significant spatial heterogeneity in the distribution of these nutrients, with no significant distance effects observed within different distance ranges from the low-tidal level to the coastline. The distribution of Suaeda glauca was closely related to the distribution of total nitrogen and pH, and differences in nitrogen and phosphorus were observed between estuary and non-estuary areas.
Article
Gastroenterology & Hepatology
Shaminie Athinarayanan, Yang-Yi Fan, Xiaokun Wang, Evelyn Callaway, Defeng Cai, Naga Chalasani, Robert S. Chapkin, Wanqing Liu
Summary: The down-regulation of FADS1 leads to hepatic lipid accumulation, affecting lipid homeostasis and fatty acid oxidation pathways, primarily mediated by the PPARα-FGF21 signaling axis.
HEPATOLOGY COMMUNICATIONS
(2021)
Article
Plant Sciences
Amit Kugler, Puja Kumari, Kamilya Kokabi, Maxim Itkin, Sergey Malitsky, Inna Khozin-Goldberg
JOURNAL OF PHYCOLOGY
(2020)
Article
Plant Sciences
Ben Leyland, Aliza Zarka, Shoshana Didi-Cohen, Sammy Boussiba, Inna Khozin-Goldberg
JOURNAL OF PHYCOLOGY
(2020)
Article
Environmental Sciences
Puja Kumari, Alon Cna'ani, Shoshana Didi-Cohen, Vered Tzin, Irma Khozin-Goldberg
FRONTIERS IN MARINE SCIENCE
(2020)
Article
Biology
Sagar Nayak, Ashraf Al Ashhab, Dina Zilberg, Inna Khozin-Goldberg
Article
Plant Sciences
Kamilya Kokabi, Olga Gorelova, Boris Zorin, Shoshana Didi-Cohen, Maxim Itkin, Sergey Malitsky, Alexei Solovchenko, Sammy Boussiba, Inna Khozin-Goldberg
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Biology
Idan Koren, Sammy Boussiba, Inna Khozin-Goldberg, Aliza Zarka
Summary: Microalgae like Chromochloris zofingiensis are plant-like micro-organisms that can capture light energy through photosynthesis to produce high value pharmaceutical and nutritional pigments. Understanding their growth characteristics is crucial for efficient commercial production. By staining the nucleus of the alga, researchers found that C. zofingiensis cells conduct consecutive DNA synthesis and divisions before dividing into daughter cells. This process is influenced by light conditions and can be applied for high pigment productivity in biotechnological applications.
Article
Plant Sciences
Avraham Ben-Sheleg, Inna Khozin-Godberg, Beery Yaakov, Avigad Vonshak
Summary: The research focused on exploring the disparity in conditions optimal for growth and EPA production in Nannochloropsis oceanica. Mutant strains resistant to oxidative stress conditions showed greater EPA production in a high light and low temperature environment compared to the wild-type strain. Mutations in specific genes were identified as potential sources of tolerance in the mutant strains.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Environmental Sciences
Ram Chandra Shrestha, Lusine Ghazaryan, Ben Poodiack, Boris Zorin, Avner Gross, Osnat Gillor, Inna Khozin-Goldberg, Ilya Gelfand
Summary: The study found that fertilization with algal biomass reduced wheat grain nitrogen content and soil nitrogen oxide emissions, leading to lower greenhouse gas emissions and global warming impact compared to urea fertilization.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Ji-Hyun Kim, Shoshana Didi-Cohen, Inna Khozin-Goldberg, Dina Zilberg
Summary: Monogenean parasites pose challenges to sustainable aquaculture due to fish morbidity and mortality. Traditional chemical treatments have limitations and risks, leading to the exploration of alternatives such as using extracts from the pennate diatom Phaeodactylum tricornutum. These extracts have shown efficacy against the monogenean parasite Gyrodactylus turnbulli affecting Poecilia reticulata, with potential applications in cultured fish for parasite control and antibacterial effects against specific bacteria.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Plant Sciences
Yogesh Taparia, Achintya Kumar Dolui, Sammy Boussiba, Inna Khozin-Goldberg
Summary: This study describes the construction of a modular two-component transcriptional unit system to express SpCas9 in a diatom. The system enables multiplexed targeting and marker-free genome editing. The editing efficiency of the system was evaluated by targeting the StLDP gene, and the results showed the importance of StLDP in lipid droplet regulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Giulia Trentin, Francesca Piazza, Marta Carletti, Boris Zorin, Inna Khozin-Goldberg, Alberto Bertucco, Eleonora Sforza
Summary: Two diazotrophic cyanobacteria were cultivated to produce cyanophycin under nitrogen fixing conditions. Nostoc sp. showed higher efficiency in accumulating cyanophycin and fixing atmospheric nitrogen. The operating conditions were optimized, with nitrogen availability and/or pH playing a major role in biomass production and phosphorus limitation maximizing cyanophycin accumulation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Ekaterina Novichkova, Sagar Nayak, Sammy Boussiba, Jacob Gopas, Dina Zilberg, Inna Khozin-Goldberg
Summary: The study investigates the anti-inflammatory and immunomodulatory potential of the microalga Lobosphaera incisa P127 in the zebrafish model of IBD. Results show that diets containing 15% non-starved (Ns) and 7.5% and 15% nitrogen-starved (St) algal biomass effectively attenuate gut injury, while diets containing 7.5% Ns and DGLA ethyl ester have no effect on gut condition.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Plant Sciences
Avital Friedjung Yosef, Lusine Ghazaryan, Linda Klamann, Katherine Sarah Kaufman, Capucine Baubin, Ben Poodiack, Noya Ran, Talia Gabay, Shoshana Didi-Cohen, Manuela Bog, Inna Khozin-Goldberg, Osnat Gillor
Summary: This study investigates the diversity of duckweeds in Israel and finds that the duckweed population in aquatic environments consists of both native and transient species. The analysis of fatty acids and protein content suggests functional differences among the identified species. Additionally, potential invasive species are identified.
Editorial Material
Plant Sciences
Zhi-Yan Du, Wajid Waheed Bhat, Guoyin Kai, Inna Khozin-Goldberg, Xiao-Hong Yu, Agnieszka Zienkiewicz, Krzysztof Zienkiewicz
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biology
Ben Leyland, Sammy Boussiba, Inna Khozin-Goldberg