Article
Cell Biology
Xiaobo Long, Nan Wang, Xinhao Zhang
Summary: The study demonstrated that CC10 can suppress the activation of ILC2s to alleviate nasal mucosa damage, and CD127+ may serve as a biomarker for the activation of ILC2s in AR mice and AR patients.
Article
Medicine, General & Internal
Elena Lopez-Rodriguez, Gemma Gay-Jordi, Lars Knudsen, Matthias Ochs, Anna Serrano-Mollar
Summary: In idiopathic pulmonary fibrosis (IPF), intratracheal administration of alveolar epithelial type II cells (AE2C) has been shown to reverse fibrosis, but further research is needed to determine if this involves any improvement in alveolar dynamics.
FRONTIERS IN MEDICINE
(2021)
Article
Medicine, General & Internal
Nicole C. Shaw, Anthony Kicic, Sue Fletcher, Stephen D. Wilton, Stephen M. Stick, Andre Schultz
Summary: ABCA-3 deficiency was studied in primary nasal epithelial cells as a surrogate culture model for AT-II cells. Expression of ABCA3, SP-B, and SP-C was detected in primary nasal epithelial cells, but at a lower level compared to AT-II cells. Immunofluorescence microscopy showed the presence of ABCA-3, SP-B, and SP-C in primary nasal epithelial cells, while western blotting did not detect SP-B and SP-C proteins. Transmission electron microscopy revealed structurally imperfect lamellar bodies in primary nasal epithelial cells.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yuliang L. Sun, Killian Hurley, Carlos Villacorta-Martin, Jessie Huang, Anne Hinds, Krithi Gopalan, Ignacio S. Caballero, Scott J. Russo, Joseph A. Kitzmiller, Jeffrey A. Whitsett, Michael F. Beers, Darrell N. Kotton
Summary: In this study, a new ABCA3:GFP reporter was generated for the purification of induced pluripotent stem cell-derived alveolar epithelial type 2 cells (iAEC2s) and visualization of organelles associated with AEC2 maturation. Comparative RNA sequencing and functional studies revealed cellular heterogeneity within the iAEC2 population, with ABCA3:GFP single-positive cells exhibiting faster growth kinetics and higher pluripotency compared to ABCA3:GFP/SFTPCtdTomato double-positive cells, which expressed more maturation markers.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Zunyong Feng, Zhou Jing, Qiang Li, Liuxi Chu, YuXin Jiang, Xuanbo Zhang, Liang Yan, Yinhua Liu, Jing Jiang, Ping Xu, Qun Chen, Ming Wang, Hui Yang, Guoren Zhou, Xiaochun Jiang, Xiaoyuan Chen, Hongping Xia
Summary: Exosomes derived from AEC-IIs, called ADEs, can balance airway immunity and alleviate damage and fibrosis by regulating the immune and metabolic status of TRAMs. In a mouse fibrosis model, supplementation with ADEs reduced acute injury, prevented advanced fibrosis, alleviated ventilatory impairment, and decreased mortality.
Article
Biology
Qianqian Pang, Chunyi Liu, Yulong Qiao, Jian Zhao, Sin Man Lam, Mei Mei, Guanghou Shui, Shilai Bao, Qiuling Li
Summary: Deletion of the GM130 gene disrupts the Golgi structure, impairing the secretion of lung surfactant proteins and lipids. This leads to an enlargement of alveolar airspace and increased alveolar epithelial autophagy, which can be partially rescued by surfactant repletion in GM130-deficient mice.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiaoman Li, Liang Wang, Jialin Hao, Qingfeng Zhu, Min Guo, Changjing Wu, Sihui Li, Qiqiang Guo, Qiuhong Ren, Ning Bai, Fei Yi, Bo Jiang, Wenyu Zhang, Yanling Feng, Hongde Xu, Han Jiang, Xiaoyue Zhai, Guohua Zhang, Hong-Long Ji, Xuesong Yang, Dan Zhang, Jianhua Fu, Jianjun Chang, Xiaoyu Song, Liu Cao
Summary: This study found that autophagy is involved in the maturation of lamellar bodies (LB) and regulates the production of surfactant proteins in alveolar epithelial cells. Changes in autophagic activity affect LB formation and function.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Editorial Material
Cell Biology
Mohammad Rudiansyah, Ermias Mergia Terefe, Maria Jade Catalan Opulencia, Walid Kamal Abdelbasset, Dmitry Olegovich Bokov, Amr A. El-Sehrawy, Sayfiddin Baymakov, Ali Thaeer Hammid, Milad Shirvaliloo, Reza Akhavan-Sigari
Summary: The histopathological alterations of AT2 cells in COVID-19 are crucial for predicting heart failure, as the EVs they release may carry viral genetic material that affects cardiac function.
INFLAMMATION RESEARCH
(2022)
Article
Immunology
Ahmed A. Al-Qahtani, Ioanna Pantazi, Fatimah S. Alhamlan, Hani Alothaid, Sabine Matou-Nasri, George Sourvinos, Eleni Vergadi, Christos Tsatsanis
Summary: The study found that the S protein of SARS-CoV-2 suppresses inflammatory responses in alveolar epithelial type II cells at early stages of infection by activating the PI3K/AKT pathway. Additionally, when combined with TLR2 signals, the S protein enhances PAI-1 expression, potentially affecting the local coagulation cascade.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Toyoshi Yanagihara, Quan Zhou, Kazuya Tsubouchi, Spencer Revill, Anmar Ayoub, Mahsa Gholiof, Sy Giin Chong, Anna Dvorkin-Gheva, Kjetil Ask, Wei Shi, Martin RJ. Kolb
Summary: Type 1 alveolar epithelial cells (AT1s) and type 2 alveolar epithelial cells (AT2s) regulate the structural integrity and function of alveoli. Gremlin, an inhibitor of bone morphogenetic protein (BMP), induces AT2 proliferation and differentiation. It was found that Gremlin expression during lung injury leads to inhibition of BMP signaling and activation of AT2s, promoting alveolar repair.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Esther Marhuenda, Alvaro Villarino, Maria Leonor Narciso, Marta Camprubi-Rimblas, Ramon Farre, Nuria Gavara, Antonio Artigas, Isaac Almendros, Jorge Otero
Summary: One of the main limitations in in vitro studies on lung diseases is maintaining the specific phenotype of alveolar epithelial cells. This study successfully maintained the phenotype of cells using lung-derived extracellular matrix hydrogels, and found that the hydrogels can promote long-term culture of alveolar epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Rafael Jesus Fernandez, Zachary J. G. Gardner, Katherine J. Slovik, Derek C. Liberti, Katrina N. Estep, Wenli Yang, Qijun Chen, Garrett T. Santini, Javier Perez, Sarah Root, Ranvir Bhatia, John W. Tobias, Apoorva Babu, Michael P. Morley, David B. Frank, Edward E. Morrisey, Christopher J. Lengner, F. Brad Johnson
Summary: DC is a rare genetic disorder that causes short telomeres and early onset of age-related diseases. Using iAT2 cells to model, it is shown that shortened telomeres lead to cell senescence and Wnt signaling defects. The study suggests that Wnt agonists may be potential therapies for DC-related pathologies.
Article
Immunology
Tengyue Zhang, Mengyuan Li, Siyuan Zhao, Mianjing Zhou, Huai Liao, Haiyan Wu, Xinyue Mo, Hongxing Wang, Chaohuan Guo, Hui Zhang, Niansheng Yang, Yuefang Huang
Summary: This study found that CaMK4 activation contributes to the activation of NLRP3 inflammasome during acute lung injury (ALI), and inhibiting CaMK4 can reduce the development of ALI and inflammation. These findings suggest that CaMK4 inhibition may be a novel therapeutic approach for the treatment of ALI.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiaoting Yang, Jing Wang, Wei Liu
Summary: In this study, molecular markers associated with type II alveolar epithelial cell injury in acute lung injury (ALI) were identified using bioinformatics methods. The results provide new insights for the diagnosis and treatment of ALI/ARDS.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Li-Chao Fan, Keith Mcconn, Maria Plataki, Sarah Kenny, Niamh C. Williams, Kihwan Kim, Jennifer A. Quirke, Yan Chen, Maor Sauler, Matthias E. Mobius, Kuei-Pin Chung, Estela Area Gomez, Augustine M. K. Choi, Jin-Fu Xu, Suzanne M. Cloonan
Summary: This study found that lipid synthesis in AEC2 cells increases with chronic smoking and that altered lipid metabolism is implicated in COPD development. The targeted deletion of the lipogenesis enzyme FASN in AEC2 cells leads to more severe smoking addiction, increased neutrophils and protein content in bronchoalveolar lavage fluid, and enlarged airspace. Additionally, AEC2 cells with deleted FASN show lower levels of surfactant phospholipids and higher levels of ether phospholipids, sphingomyelins, and polyunsaturated fatty acid-containing phospholipids in the lung fluid, as well as increased surface tension.
Article
Rheumatology
Haoruo Jia, Xiaoyuan Ma, Yulong Wei, Wei Tong, Robert J. Tower, Abhishek Chandra, Luqiang Wang, Zeyang Sun, Zhaochun Yang, Farid Badar, Kairui Zhang, Wei-Ju Tseng, Ina Kramer, Michaela Kneissel, Yang Xia, X. Sherry Liu, James H. C. Wang, Lin Han, Motomi Enomoto-Iwamoto, Ling Qin
ARTHRITIS & RHEUMATOLOGY
(2018)
Article
Endocrinology & Metabolism
Luqiang Wang, Robert J. Tower, Abhishek Chandra, Lutian Yao, Wei Tong, Zekang Xiong, Kai Tang, Yejia Zhang, X. Sherry Liu, Joel D. Boerckel, Xiaodong Guo, Jaimo Ahn, Ling Qin
JOURNAL OF BONE AND MINERAL RESEARCH
(2019)
Article
Endocrinology & Metabolism
Abhishek Chandra, Anthony B. Lagnado, Joshua N. Farr, David G. Monroe, Sean Park, Christine Hachfeld, Tamar Tchkonia, James L. Kirkland, Sundeep Khosla, Joao F. Passos, Robert J. Pignolo
JOURNAL OF BONE AND MINERAL RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Akane Sakaguchi, Chihiro Nishiyama, Wataru Kimura
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2020)
Review
Pharmacology & Pharmacy
Jyotika Rajawat, Abhishek Chandra
Summary: Activation of PARP1 is involved in inflammatory and viral diseases, including Covid-19, and inhibiting PARP1 may attenuate inflammatory cytokine expression. Literature suggests that PARP1 plays a key role in regulating viral replication and virulence, with potential therapeutic implications for Covid-19 and other viral infections.
CURRENT DRUG TARGETS
(2021)
Review
Biochemistry & Molecular Biology
Abhishek Chandra, Jyotika Rajawat
Summary: Aging leads to tissue dysfunction and imbalance in bone homeostasis, contributing significantly to age-related osteoporosis. Cellular senescence and the SASP are discussed as key factors in aged skeleton and provide potential targets for treating and preventing osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Naoki Iwasa, Takeshi K. Matsui, Naohiko Iguchi, Kaoru Kinugawa, Naritaka Morikawa, Yoshihiko M. Sakaguchi, Tomo Shiota, Shinko Kobashigawa, Mari Nakanishi, Masaya Matsubayashi, Riko Nagata, Sotaro Kikuchi, Tatsuhide Tanaka, Nobuyuki Eura, Takao Kiriyama, Tesseki Izumi, Kozue Saito, Hiroshi Kataoka, Yuichi Saito, Wataru Kimura, Akio Wanaka, Yuhei Nishimura, Eiichiro Mori, Kazuma Sugie
Summary: This study utilized cerebral organoids derived from human induced pluripotent stem cells to evaluate the impact of ischemic stroke on human cerebral tissue. Pathway analysis revealed significant relationships between various metabolic pathways and the complement and coagulation cascades. Key markers of neuronal cells in response to OGD/R were identified as the fatty acids-related PPAR signaling pathway and pyruvate kinase isoform M2 (PKM2).
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Akane Sakaguchi, Wataru Kimura
Summary: Most adult mammalian hearts cannot regenerate after injury due to the lack of cardiomyocyte proliferation, while some vertebrates like zebrafish and urodele amphibians, as well as fetal and early neonatal mammals, retain this ability, supporting tissue regeneration and cardiac function recovery. Understanding the evolutionarily conserved regulatory mechanisms of cardiomyocyte proliferation and their modifiability for cardiac regeneration in adult mammals is of great scientific and medical interest. Recently, environmental hypoxia, hypoxia-induced cellular signaling, and mitochondrial metabolism have been recognized as crucial regulators of cardiomyocyte cell cycle and cardiac regeneration in vertebrates.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Satoshi Kawakami, Hikari Yoshitane, Taiki Morimura, Wataru Kimura, Yoshitaka Fukada
Summary: The deficiency of the Lmna gene causes a shift in mice behavior to daytime and affects the phase angle between the central clock and peripheral clocks, while maintaining molecular oscillation and phase resetting mechanism intact. High-fat diet extends the lifespan of deficient mice, improving behavioral abnormalities and phase of peripheral clocks.
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Cell Biology
Abhishek Chandra, Anthony B. Lagnado, Joshua N. Farr, Madison Doolittle, Tamara Tchkonia, James L. Kirkland, Nathan K. LeBrasseur, Paul D. Robbins, Laura J. Niedernhofer, Yuji Ikeno, Joao F. Passos, David G. Monroe, Robert J. Pignolo, Sundeep Khosla
Summary: This study demonstrates that cellular senescence-driven radiation-induced osteoporosis is primarily mediated by p21(Cip1) rather than p16(Ink4a), based on the clearance of senescent cells using genetic models. This approach may be used to investigate the contributions of these pathways in other senescence-associated conditions, including aging across tissues.
Article
Endocrinology & Metabolism
Abhishek Chandra, Anthony B. Lagnado, Joshua N. Farr, Megan Schleusner, David G. Monroe, Dominik Saul, Joao F. Passos, Sundeep Khosla, Robert J. Pignolo
Summary: Oxidative stress-induced cellular senescence is associated with the accumulation of bone marrow adipocytes, but the direct link between cellular senescence and bone marrow adipocyte tissue (BMAT) remains elusive. This study demonstrates that radiation and aging induce the occurrence of BMAT through a common pathway that involves the presence of senescent cells. Clearance of senescent cells reduces BMAT in aged and radiated bones.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Review
Gastroenterology & Hepatology
Vy Truong Thuy Nguyen, Negar Taheri, Abhishek Chandra, Yujiro Hayashi
Summary: Aging is a complex process that affects the functions of the gastrointestinal tract. Age-related GI motor disorders can negatively impact quality of life and increase the risk of other diseases. However, the mechanisms underlying these disorders and the effect of aging on GI motor functions are still not well understood, necessitating further research.
NEUROGASTROENTEROLOGY AND MOTILITY
(2022)
Article
Cardiac & Cardiovascular Systems
Chihiro Nishiyama, Yuichi Saito, Akane Sakaguchi, Mari Kaneko, Hiroshi Kiyonari, Yuqing Xu, Yuichiro Arima, Hideki Uosaki, Wataru Kimura
Summary: The marsupial opossum is capable of maintaining cardiomyocyte proliferation and myocardial regeneration for at least 2 weeks after birth, which is the longest postnatal duration of such a capacity among mammals examined to date.
Article
Dermatology
Yasushi Kikuchi, Tomoki Tamakoshi, Ryuichi Ishida, Ryosuke Kobayashi, Shiho Mori, Akemi Ishida-Yamamoto, Manabu Fujimoto, Yasufumi Kaneda, Katsuto Tamai
Summary: In this study, researchers developed an ex vivo gene therapy for recessive dystrophic epidermolysis bullosa (RDEB) using autologous mesenchymal stromal cells (MSCs). The gene-modified MSCs were injected into mice with type VII collagen deficiency, leading to continuous and widespread expression of type VII collagen. The therapy showed successful application in both early blistering skin and advanced ulcerative lesions in the RDEB mouse model.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Review
Endocrinology & Metabolism
Robert J. Pignolo, Susan F. Law, Abhishek Chandra
Summary: This study focuses on the physiological changes in aging bones and the role of cellular senescence in age-related osteoporosis. It reviews different aspects of bone aging, including remodeling deficits, uncoupling phenomena, and the accumulation of senescent cells in the bone microenvironment, as major mechanisms for age-related osteoporosis. The study also discusses the potential therapeutic interventions based on clearing senescent cells or suppressing the senescence-associated secretory phenotype.
Article
Medicine, Research & Experimental
Yuwen Zhu, Yan Guo, Yujia Xue, Anqi Zhou, Ying Chen, Yifei Chen, Xiulian Miao, Fangqiao Lv
Summary: BRG1 plays an important role in HSC-myofibroblast transition and targeting it could be a reasonable strategy for liver fibrosis intervention.
Article
Medicine, Research & Experimental
Liu Ye, Beibei Liu, Jingling Huang, Xiaolin Zhao, Yuan Wang, Yungen Xu, Shuping Wang
Summary: Doublecortin-like kinase 1 (DCLK1) is a significant prooncogenic factor that is strongly associated with the malignant progression and clinical prognosis of various cancers. DCLK1 plays important roles in stem cell marker regulation, tumor cell reprogramming, and immune evasion. However, the exact biological functions of DCLK1, especially the disparities between its alpha- and beta-form transcripts in cancer progression, remain ambiguous.
Article
Medicine, Research & Experimental
Jiahui Yang, Xiaoyu Chen, Tianjing Liu, Yongyan Shi
Summary: This article reviews the role of bile acids in necrotizing enterocolitis (NEC) and their potential therapeutic value. The dysregulation of bile acids is associated with intestinal injury, and inflammatory factors in the liver also play a crucial role in regulating bile acid transport. The bile acid metabolic pathway is important for regulating intestinal microbiota, cell proliferation, and barrier protection.
Review
Medicine, Research & Experimental
Zhenzheng Zhu, Yuemiao Xu, Yuwei Xia, Xinru Jia, Yixin Chen, Yuyue Liu, Leyin Zhang, Hui Chai, Leitao Sun
Summary: Bile acid, as the final product of cholesterol breakdown, plays a complex regulatory and signaling role in human metabolism. Research suggests that it has the potential to enhance metabolism and regulate chronic metabolic diseases through various pathways. The interaction between bile acid and gut microbiota is also of great significance.
Article
Medicine, Research & Experimental
Xin He, Hong-Xu Zhou, Xian Fu, Kai-Di Ni, Ai-Zhi Lin, Ling-Tong Zhang, Hou-Hua Yin, Qing Jiang, Xue Zhou, Yi-Wen Meng, Jun-Yan Liu
Summary: DON exposure causes an increase in deoxycholic acid (DCA), which contributes to intestinal injury. DCA may be a potential therapeutic target for DON enterotoxicity.
Article
Medicine, Research & Experimental
Zhitao Wang, Heng Ma, Abdul Nasir, Sufang Liu, Zhisong Li, Feng Tao, Qian Bai
Summary: This study reveals the involvement of TET1-mediated epigenetic regulation in chronic TMJ pain through trigeminal TNF alpha signaling.
Article
Medicine, Research & Experimental
Lu Yu, Hao Ran, Yaru Lu, Qian Ma, Huan Huang, Weibin Liu
Summary: This study found that the HIF-1 alpha inhibitor BAY 87-2243 can alleviate the symptoms of the Experimental Autoimmune Myasthenia Gravis (EAMG) inflammation model. BAY 87-2243 can restore the balance of CD4(+)T cell subsets, reduce the production of pro-inflammatory cytokines, and act as both an immune imbalance regulator and anti-inflammatory.
Article
Medicine, Research & Experimental
Alex Peralvarez-Marin, Montse Sole, Judith Serrano, Alice Taddeucci, Belen Perez, Clara Penas, Gemma Manich, Marcel Jimenez, Pilar D'Ocon, Francesc Jimenez-Altayo
Summary: This study provides the first evidence that TRPV2 channels may modulate vascular tone by balancing opposing inputs from the endothelium and smooth muscle, leading to net vasodilation. The amplification of TRPV2 channel-induced activity by NO emphasizes the pathophysiological relevance of these findings.
Article
Medicine, Research & Experimental
Amin Ullah, Jing Zhao, Jiakun Li, Rajeev K. Singla, Bairong Shen
Summary: Gastric cancer is the fifth-most prevalent and second-most deadly cancer worldwide. Late onset of symptoms makes early detection important. CXC chemokines play an important role in the pathological process of gastric cancer, but their exact role in diagnosis and prognosis is not fully understood. Inhibiting CXC chemokines shows promise as a targeted therapy.
Article
Medicine, Research & Experimental
Menna S. Zeyada, Salma M. Eraky, Mamdouh M. El-Shishtawy
Summary: The current study demonstrates the prophylactic and antifibrotic effects of Trig against BLM-induced PF by targeting multiple signaling pathways. The combination of Trig and Pirf may be a promising approach to enhance Pirf's anti-fibrotic effect.
