4.1 Article

Testing morphology-based delimitation of Vulpicida juniperinus and V. tubulosus (Parmeliaceae) using three molecular markers

Journal

LICHENOLOGIST
Volume 44, Issue 6, Pages 757-772

Publisher

CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0024282912000448

Keywords

gene tree; ITS; lichenized fungi; Mcm7; molecular phylogenetics; MrBayes; mtSSU; taxonomy

Funding

  1. Estonian Science Foundation [JD173, 7470, 9109]
  2. European Union through European Regional Development Fund (Center of Excellence FIBIR)

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The delimitation of two morphologically similar and not easily separable Vulpicida species, V. juniperinus and V. tubulosus, is analyzed using nuclear ITS and Mcm7, and mitochondrial SSU DNA sequences. Seventy-nine Vulpicida specimens, most from the two focal taxa, are included in the three-locus gene tree. The results from Bayesian and parsimony analyses are presented. There are strong conflicts between the single locus gene trees. Vulpicida juniperinus and V. tubulosus are divided into two clearly distinguished groups in the ITS and concatenated B/MCMC tree. However, these species are mixed in both clades, appearing polyphyletic. Currently accepted V. juniperinus and V. tubulosus are not distinct according to the loci studied. Vulpicida pinastri appears monophyletic based on the available sequences.

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