Article
Cell & Tissue Engineering
Sara Bucar, Andre Dargen de Matos Branco, Marcia F. Mata, Joao Coutinho Milhano, Iris Caramalho, Joaquim M. S. Cabral, Ana Fernandes-Platzgummer, Claudia L. da Silva
Summary: The study compared the capacity of MSC from different sources to expand UCB CD34(+) cells ex vivo, with AT-derived MSC showing superior expansion potential and maintenance of primitive cells. UCM-derived MSC demonstrated inferior hematopoietic supportive capacity compared to MSC from adult tissues. Further research is needed to determine the impact of HPL on the hematopoietic supportive capacity of MSC.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Abisha Crystal Christopher, Vigneshwaran Venkatesan, Karthik V. Karuppusamy, Saranya Srinivasan, Prathibha Babu, Manoj Kumar K. Azhagiri, Karthik Chambayil, Abhirup Bagchi, Vignesh Rajendiran, Nithin Sam Ravi, Sanjay Kumar, Srujan Kumar Marepally, Kumarasamypet Murugesan Mohankumar, Alok Srivastava, Shaji R. Velayudhan, Saravanabhavan Thangavel
Summary: In this study, a combination of small molecules was identified to selectively expand CD34(+)CD133(+)CD90(+) hematopoietic stem cells (HSCs) in ex vivo culture. The preferential expansion enriched the HSCs in culture and significantly increased long-term engraftment in NSG mice. The culture-enriched HSCs also showed a higher responsiveness to gene modification, resulting in a higher frequency of gene-modified HSCs. The yield of gene-modified HSCs obtained through culture enrichment was similar to sort-purification and superior to Cyclosporin-H treatment.
HUMAN GENE THERAPY
(2022)
Article
Immunology
Sabrina B. Bennstein, Sandra Weinhold, Oezer Degistirici, Robert A. J. Oostendorp, Katharina Raba, Gesine Koegler, Roland Meisel, Lutz Walter, Markus Uhrberg
Summary: ILC3, particularly IL-22-secreting ILC3, play vital roles in maintaining mucosal barrier functions and controlling inflammatory diseases. A method for generating functionally competent human ILC3 from cord blood-derived CD34(+) hematopoietic progenitors on human MSCs has been developed, showing promise for potential clinical applications in treating inflammatory diseases and cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Wanbo Tang, Jian He, Tao Huang, Zhijie Bai, Chaojie Wang, Haizhen Wang, Ruichuang Yang, Yanli Ni, Jun Hou, Junliang Wang, Jie Zhou, Yingpeng Yao, Yandong Gong, Siyuan Hou, Bing Liu, Yu Lan
Summary: Using gene knockin strategy, a new Hlf-tdTomato reporter mouse model was developed that revealed the importance of Hlf expression in the generation of functional HSCs from early pre-HSCs. The construction of a novel Hlf-CreER mouse model enabled genetic lineage tracing showing the contribution of early cells to multi-lineage hematopoiesis in the bone marrow. This study provides valuable tools to specifically trace the fate of emerging HSCs during development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Guoyi Dong, Xiaojing Xu, Yue Li, Wenjie Ouyang, Weihua Zhao, Ying Gu, Jie Li, Tianbin Liu, Xinru Zeng, Huilin Zou, Shuguang Wang, Yue Chen, Sixi Liu, Hai-Xi Sun, Chao Liu
Summary: In this study, single-cell RNA-seq was used to analyze hematopoietic stem cells (HSCs) from cord blood and mobilized peripheral blood. The source and culture time of HSCs were found to affect their characteristics, and stemness-related genes were identified. The study revealed that maintaining stemness requires dynamic protein translation and processing, and culture time also affects HSCs' homing and engraftment ability. Furthermore, the study identified a small molecule that can expand HSCs while maintaining their stemness.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Review
Hematology
Fernando Anjos-Afonso, Dominique Bonnet
Summary: The ability to isolate and characterize different hematopoietic stem cell (HSC) or progenitor cell populations provides insights into the regulation of hematopoiesis in various conditions. While significant progress has been made in understanding the composition of these cell types in mice, recent breakthroughs have improved the resolution of the human primitive hematopoietic compartment. This review aims to provide a historical perspective and discuss the progress in characterizing human postnatal CD34(+) HSC-enriched populations, with potential implications for future translational studies.
Article
Cell & Tissue Engineering
Huangfan Xie, Zhongjie Sun, Xiong Xiao, Defang Liu, Hailong Qi, Guoxiong Tian, Miao Chen, Ligong Chen, Xuncheng Su
Summary: Transient inhibition of the JNK pathway can increase the frequency of HSCs in cord blood and promote their quiescence, leading to the enhancement of HSC number and engraftment potential. These findings have the potential to improve HSC collection and transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Pathology
Sandra Bluemich, Hana Zdimerova, Christian Muenz, Anja Kipar, Giovanni Pellegrini
Summary: Immunodeficient mice engrafted with human immune cells showed various pathological features, with one mouse developing mediastinal lymphoblastic lymphoma. Common lesions in mice engrafted with human HSC include nephropathy, ovarian atrophy, cataract, and abnormal retinal development.
VETERINARY PATHOLOGY
(2021)
Article
Hematology
Isabel C. Yoon, Nicholas A. Bascou, Michele D. Poe, Paul Szabolcs, Maria L. Escolar
Summary: HSCT can prolong the lifespan and improve the functional abilities of late-infantile patients with Krabbe disease, especially those who undergo transplantation before symptom onset. The cognitive outcomes were better for patients with disease onset at >12 months compared to untreated patients. The findings support reclassifying late-infantile Krabbe disease to symptom onset at 12 to 36 months of age.
Article
Cell & Tissue Engineering
Richa Kaushal, Suria Jahan, Chelsea McGregor, Nicolas Pineault
Summary: This study compared the cryopreservation capacity of four DMSO-free freezing media for cord blood HSCs. The results showed that CPP-STEM, a DMSO-free freezing medium, achieved the best post-thaw cell viability, recovery of viable CD45+ and CD34+ cells, and potency. It was equal or superior to the control DMSO. CSL provided the second best results, followed by PIM, while CN had modest viability and potency. Further investigations confirmed that CB CD34-enriched HSCs and progenitors cryopreserved with CPP-STEM maintained high viability and growth expansion activity, supporting normal short-term and long-term engraftment kinetics.
Article
Genetics & Heredity
Takahiro Sano, Koki Ueda, Keiji Minakawa, Tsutomu Mori, Yuko Hashimoto, Haruhiko Koseki, Yasuchika Takeishi, Kazuhiko Ikeda, Takayuki Ikezoe
Summary: This study generated Uhrf2(-/-) mice to investigate the role of UHRF2 deletion in hematopoiesis. The results showed that the absence of UHRF2 did not affect blood counts, bone marrow findings, and spleen weights. However, the proportions of Uhrf2(-/-) cells were decreased compared to Uhrf2(+/+) cells in competitive repopulation assays, indicating a potential regulatory role of UHRF2 in hematopoiesis.
Article
Biochemistry & Molecular Biology
Yiqi Yang, Bihui Zhang, Junye Xie, Jingsheng Li, Jia Liu, Rongzhan Liu, Linhao Zhang, Jinting Zhang, Zijian Su, Fu Li, Leisheng Zhang, An Hong, Xiaojia Chen
Summary: The CH02 peptide is effective in ex vivo expansion of CD34(+) umbilical cord blood hematopoietic stem/progenitor cells and promotes wound healing in diabetic mice through bidirectional orchestration of proinflammatory and anti-inflammatory factors.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Hematology
Madhavi Lakkaraja, Michael Scordo, Audrey Mauguen, Christina Cho, Sean Devlin, Josel D. Ruiz, Elizabeth Klein, Scott T. Avecilla, Farid Boulad, Maria Cancio, Kevin J. Curran, Ann A. Jakubowski, Nancy A. Kernan, Andrew L. Kung, Richard J. O'Reilly, Esperanza B. Papadopoulos, Susan Prockop, Ichelle van Roessel, Andromachi Scaradavou, Brian C. Shaffer, Gunjan Shah, Barbara Spitzer, Roni Tamari, Sergio A. Giralt, Miguel-Angel Perales, Jaap Jan Boelens
Summary: The traditional weight-based dosing in rabbit antithymocyte globulin (rATG) treatment leads to variable clearance and higher mortality. A personalized pharmacokinetic-guided rATG dosing strategy can improve patient survival.
Article
Hematology
Kristin M. Page, Margie A. Ream, Hemalatha G. Rangarajan, Rafael Galindo, Ali Y. Mian, Mai-Lan Ho, James Provenzale, Kathryn E. Gustafson, Jennifer Rubin, Shalini Shenoy, Joanne Kurtzberg
Summary: Infantile Krabbe disease can be effectively treated with hematopoietic cell transplantation if done during the first weeks of life. Newborn screening has facilitated early diagnosis and streamlined treatment referrals for this disease. A retrospective review of six infants diagnosed through newborn screening and treated with hematopoietic cell transplantation showed successful engraftment and improved neurodevelopmental outcomes. All infants are alive and deriving benefits from the timely treatment.
Review
Immunology
Mary Slatter, Su Han Lum
Summary: Patients with inborn errors of immunity (IEI) have undergone transplantation for more than 50 years. The need for further improvements in hematopoietic stem cell transplantation (HSCT) is shown by ongoing medical issues in long-term survivors. Precise genetic diagnosis allows early treatment, and specific biologic therapies can be used as a bridge to improve outcomes. Advances in reduced toxicity conditioning and methods of T-cell depletion have made transplant an option for all eligible patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Ruri Kato, Hiroya Tamaki, Kazuhiro Ikegame, Satoshi Yoshihara, Katsuji Kaida, Kyoko Taniguchi, Takayuki Inoue, Shinichi Ishii, Jun Nakata, Tatsuya Fujioka, Ryoji Eguchi, Toshihiro Soma, Masaya Okada, Hiroyasu Ogawa
ANNALS OF HEMATOLOGY
(2015)
Article
Cell & Tissue Engineering
Ryusuke Nakatsuka, Yoshikazu Matsuoka, Yasushi Uemura, Keisuke Sumide, Ryuji Iwaki, Masaya Takahashi, Tatsuya Fujioka, Yutaka Sasaki, Yoshiaki Sonoda
CELL TRANSPLANTATION
(2015)
Article
Cell & Tissue Engineering
Yoshikazu Matsuoka, Ryusuke Nakatsuka, Keisuke Sumide, Hiroshi Kawamura, Masaya Takahashi, Tatsuya Fujioka, Yasushi Uemura, Hiroaki Asano, Yutaka Sasaki, Masami Inoue, Hiroyasu Ogawa, Takayuki Takahashi, Masayuki Hino, Yoshiaki Sonoda
Letter
Oncology
Y. Matsuoka, K. Sumide, H. Kawamura, R. Nakatsuka, T. Fujioka, Y. Sasaki, Y. Sonoda
BLOOD CANCER JOURNAL
(2015)
Letter
Hematology
Yoshikazu Matsuoka, Keisuke Sumide, Hiroshi Kawamura, Ryusuke Nakatsuka, Tatsuya Fujioka, Yoshiaki Sonoda
Article
Cell & Tissue Engineering
Ryusuke Nakatsuka, Ryuji Iwaki, Yoshikazu Matsuoka, Keisuke Sumide, Hiroshi Kawamura, Tatsuya Fujioka, Yutaka Sasaki, Yasushi Uemura, Hiroaki Asano, A-Hon Kwon, Yoshiaki Sonoda
STEM CELLS AND DEVELOPMENT
(2016)
Article
Biophysics
S. Yoshihara, K. Ikegame, K. Taniguchi, K. Kaida, E. H. Kim, J. Nakata, R. Kato, T. Inoue, T. Fujioka, H. Tamaki, M. Okada, T. Soma, H. Ogawa
BONE MARROW TRANSPLANTATION
(2012)
Article
Biophysics
S. Yoshihara, K. Ikegame, K. Kaida, K. Taniguchi, R. Kato, T. Inoue, T. Fujioka, H. Tamaki, M. Okada, T. Soma, H. Ogawa
BONE MARROW TRANSPLANTATION
(2012)
Article
Biophysics
S. Yoshihara, E. Maruya, K. Taniguchi, K. Kaida, R. Kato, T. Inoue, T. Fujioka, H. Tamaki, K. Ikegame, M. Okada, T. Soma, K. Hayashi, N. Fujii, T. Onuma, Y. Kusunoki, H. Saji, H. Ogawa
BONE MARROW TRANSPLANTATION
(2012)
Article
Biophysics
T. Fujioka, H. Tamaki, K. Ikegame, S. Yoshihara, K. Taniguchi, K. Kaida, R. Kato, T. Inoue, J. Nakata, S. Ishii, T. Soma, M. Okada, H. Ogawa
BONE MARROW TRANSPLANTATION
(2013)
Article
Biophysics
K. Taniguchi, S. Yoshihara, E. Maruya, K. Ikegame, K. Kaida, K. Hayashi, R. Kato, T. Inoue, T. Fujioka, H. Tamaki, M. Okada, T. Onuma, N. Fujii, Y. Kusunoki, T. Soma, H. Saji, H. Ogawa
BONE MARROW TRANSPLANTATION
(2012)
Article
Hematology
Kazuhiro Ikegame, Katsuji Kaida, Satoshi Yoshihara, Masayuki Fujiwara, Kyoko Taniguchi, Ruri Kato, Takayuki Inoue, Tatsuya Fujioka, Hiroya Tamaki, Masaya Okada, Toshihiro Soma, Norihiko Kamikonya, Hiroh Saji, Shozo Hirota, Hiroyasu Ogawa
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2012)
Article
Hematology
Kazuhiro Ikegame, Ruri Kato, Tatsuya Fujioka, Masaya Okada, Katsuji Kaida, Shinichi Ishii, Satoshi Yoshihara, Takayuki Inoue, Kyoko Taniguchi, Hiroya Tamaki, Toshihiro Soma, Hiroyasu Ogawa
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2013)
Article
Cell & Tissue Engineering
Yoshikazu Matsuoka, Masaya Takahashi, Keisuke Sumide, Hiroshi Kawamura, Ryusuke Nakatsuka, Tatsuya Fujioka, Yoshiaki Sonoda
CELL TRANSPLANTATION
(2017)