Article
Chemistry, Analytical
Guoxia Zheng, Qian Gao, Youwei Jiang, Ling Lu, Jianfeng Li, Xingcai Zhang, Hongyu Zhao, Panpan Fan, Yutong Cui, Furong Gu, Yunhua Wang
Summary: By combining digital microfluidic (DMF) with loop-mediated isothermal amplification (LAMP), an automated instrument-compact DMF-LAMP device was developed for rapid and sensitive detection of Vibrio parahaemolyticus. Proper mixing was found to enhance the amplification reaction rate, shorten the amplification time, and improve detection sensitivity of the device. The device showed high sensitivity with a detection limit of <0.23 x 10(3) CFU/g, making it a promising sample-to-answer system for quick and sensitive analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma
Summary: BNNPs have been proven to significantly improve the specificity and sensitivity of PCR and LAMP amplification reactions, expanding the application range of nucleic acid detection and increasing detection sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agronomy
Guanghui Kong, Tinglu Li, Weixiong Huang, Minhui Li, Wankuan Shen, Liqun Jiang, Tom Hsiang, Zide Jiang, Pinggen Xi
Summary: This study developed a highly sensitive detection method targeting P. litchii for early diagnosis on lychee. The method showed higher sensitivity than conventional PCR and could detect the presence of P. litchii in leaves, fruits, and soils.
Article
Microbiology
Ahmad Mukhlis Abdul Rahman, Julian Ransangan, Vijay Kumar Subbiah
Summary: A rapid, simple, sensitive, and specific detection method based on loop-mediated isothermal amplification (LAMP) has been developed for the detection of Vibrio harveyi contamination. The addition of loop primers reduces the reaction time of LAMP by more than half, and with the use of SYBR Green, results can be obtained within 10 to 15 minutes without gel electrophoresis. LAMP has greater sensitivity compared to PCR.
Article
Food Science & Technology
Yuting Shang, Qinghua Ye, Shuzhen Cai, Qingping Wu, Rui Pang, Shuanghong Yang, Xinran Xiang, Chufang Wang, Fei Zha, Yu Ding, Yinzhi Zhang, Jiasheng Wang, Xiulan Sun, Jumei Zhang
Summary: New molecular targets for Salmonella detection were identified through bioinformatics analysis and PCR verification. The ssaQ gene was selected as the target gene for LAMP-based detection, showing 10-fold higher sensitivity compared to the optimal PCR method.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biology
Sara M. Elnomrosy, Naglaa M. Hagag, Mohamed I. AbdAllah, Rafal Kolenda, Maciej Zacharski
Summary: This study compared the performance of LAMP and PCR techniques for sex identification of 21 parrot species, showing that LAMP, especially with PSI-W primers, is suitable for previously untested species and is more sensitive for female sex identification.
Article
Plant Sciences
Qingcong He, Dongwei Wang, Bei Tang, Jian Wang, Deyong Zhang, Yong Liu, Feixue Cheng
Summary: In this study, specific primers were designed for rapid identification and quantification of the plant-parasitic nematode Meloidogyne graminicola in soil. Both LAMP and real-time PCR showed higher sensitivity in detecting the nematode compared to conventional PCR. These methods have the potential to provide a useful platform for rapid species identification and quantification of economically important PPNs.
Article
Medicine, General & Internal
Ashiq Hussain, Muhammad Kamran Taj, Muhammad Shafi Khosa, Bezan Baloch, Muhammad Tahir, Abdul Sadiq
Summary: The study investigated the epidemiology of Vibrio cholera and the identification of strains using polymerase chain reaction. Results showed a higher incidence of Vibrio cholera in individuals aged 1-20, males, Balochs, lower socioeconomic class, and illiterates. The PCR method was highly effective in diagnosing the strains. Surveillance of diarrhoeal disorders is crucial for controlling cholera outbreaks in the region.
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION
(2021)
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Yu Zhang, Xinyue Li, Shuya Zhang, Tianling Ma, Chengxin Mao, Chuanqing Zhang
Summary: A quantitative loop-mediated isothermal amplification (q-LAMP) method was developed for Ustilaginoidea virens detection and quantification, which is more sensitive and efficient than quantitative real-time PCR (q-PCR) method. The q-LAMP assay could detect a concentration of 6.4 spores/mL at an optimal reaction temperature of 63.4℃ within 60 minutes. It is a powerful and simple monitoring tool for U. virens, providing valuable technical support for the forecast and management of rice false smut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Anto J. U. K. John, Peijun J. W. He, Ioannis N. Katis, P. P. Galanis, Alice H. Iles, Robert W. Eason, Collin L. Sones
Summary: With the ongoing SARS-CoV-2 pandemic, the development of rapid and cost-effective diagnostic methods has become crucial. A testing strategy utilizing a sensitive RT-LAMP assay in commercially available glass capillaries has been developed, providing results in a short timeframe and reducing the burden on testing supply chains.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Oncology
Orapan Manajit, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
MOLECULAR MEDICINE REPORTS
(2018)
Article
Fisheries
Pradit Wangman, Siwaporn Longyant, Suparat Taengchaiyaphum, Saengchan Senapin, Paisarn Sithigorngul, Parin Chaivisuthangkura
Article
Fisheries
Tanapon Soonthonsrima, Pradit Wangman, Parin Chaivisuthangkura, Chalinan Pengsuk, Paisarn Sithigorngul, Siwaporn Longyant
AQUACULTURE RESEARCH
(2019)
Article
Fisheries
Akapon Vaniksampanna, Siwaporn Longyant, Walaiporn Charoensapsri, Paisarn Sithigorngul, Parin Chaivisuthangkura
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Biophysics
Jantana Kampeera, Patiya Pasakon, Chanpen Karuwan, Narong Arunrut, Assawapong Sappat, Sarawut Sirithammajak, Nipaphorn Dechokiattawan, Thitinun Sumranwanich, Parin Chaivisuthangkura, Puey Ounjai, Surang Chankhamhaengdecha, Anurat Wisitsoraat, Adisorn Tuantranont, Wansika Kiatpathomchai
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Biotechnology & Applied Microbiology
Phongthana Pasookhush, Charles Hindmarch, Paisarn Sithigorngul, Siwaporn Longyant, William G. Bendena, Parin Chaivisuthangkura
Article
Fisheries
Pradit Wangman, Parin Chaivisuthangkura, Suparat Taengchaiyaphum, Chalinan Pengsuk, Paisarn Sithigorngul, Siwaporn Longyant
JOURNAL OF FISH DISEASES
(2020)
Article
Fisheries
Sarocha Jitrakorn, Warachin Gangnonngiw, Malinee Bunnontae, Orapan Manajit, Triwit Rattanarojpong, Parin Chaivisuthangkura, Ha Thanh Dong, Vanvimon Saksmerprome
Article
Fisheries
Phongthana Pasookhush, Akapon Vaniksampanna, Paisarn Sithigorngul, Siwaporn Longyant, Parin Chaivisuthangkura
AQUACULTURE INTERNATIONAL
(2020)
Article
Biotechnology & Applied Microbiology
Chalinan Pengsuk, Pradit Wangman, Parin Chaivisuthangkura, Paisarn Sithigorngul, Siwaporn Longyant
Summary: The study developed a nanogold-based immunochromatographic strip test for rapid and accurate detection of various Vibrio cholerae strains, suitable for preliminary detection of cholera pathogens in fresh seafood. While detection sensitivity can be enhanced through preincubation, the presence of other bacteria in the sample may influence the test results.
JOURNAL OF FOOD SAFETY
(2021)
Article
Fisheries
Terawut Prasitporn, Saengchan Senapin, Akapon Vaniksampanna, Siwaporn Longyant, Parin Chaivisuthangkura
Summary: The study introduced a new method for detecting SDDV virus, utilizing UDG-assisted CPA combined with HNB and LFD technologies, which proved to be rapid, simple, and effective.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Chutima Srisuk, Chanitcha Choolert, William G. Bendena, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
Summary: This study isolated the cDNA of hemocyanin isoform 2 of the prawn Macrobrachium rosenbergii (MrHc2) and characterized its mRNA expression. The study found that MrHc2 contains three domains and was significantly expressed when challenged with MrNV, suggesting its important role in the innate immune response of M. rosenbergii to MrNV.
JOURNAL OF AQUATIC ANIMAL HEALTH
(2022)
Article
Fisheries
Chanitcha Choolert, Phongthana Pasookhush, Akapon Vaniksampanna, Siwaporn Longyant, Parin Chaivisuthangkura
Summary: In this study, a homolog of TRAF6 called MrTRAF6 was identified and characterized. It was found that MrTRAF6 is involved in various biological processes, including adaptive and innate immunity. Gene expression analysis showed that MrTRAF6 is expressed in all tissues and plays a critical role in antibacterial activity.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Education & Educational Research
Supakan Buatip, Parin Chaivisuthangkura, Pinit Khumwong
INTERNATIONAL JOURNAL OF INSTRUCTION
(2019)
Article
Fisheries
Chutima Srisuk, Saengchan Senapin, William G. Bendena, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
AQUACULTURE RESEARCH
(2018)