Article
Biochemical Research Methods
Radu Hristu, Stefan G. Stanciu, Adrian Dumitru, Bogdan Paun, Iustin Floroiu, Mariana Costache, George A. Stanciu
Summary: Second harmonic generation (SHG) microscopy is a powerful tool for tissue characterization and diagnostics in medicine, particularly for mapping collagen structure for diagnosis. The study found that H&E staining affects the extraction and use of image parameters specific to polarization-resolved SHG microscopy, potentially leading to different analysis results and impacting diagnostic accuracy.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Chemistry, Multidisciplinary
Yanan Sun, Lishuang Li, Shuhua Ma, Gaiying He, Weifeng Yang, Yi Wang
Summary: The transdermal administration of collagen is an important method for wound healing and skin regeneration. This study presents a novel approach that combines second-harmonic generation with two-photon excited fluorescence to visualize the dynamics of collagen transdermal absorption in vivo. The findings were validated through traditional in vitro skin scanning and histological examination.
FRONTIERS IN CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Shenyi Jiang, Shuhao Qian, Lingxi Zhou, Jia Meng, Rushan Jiang, Chuncheng Wang, Xinguo Fang, Chen Yang, Zhihua Ding, Shuangmu Zhuo, Zhiyi Liu
Summary: The study uses label-free multiphoton microscopy to investigate the collagen and elastin fibers in human skin specimens and proposes a multi-fiber metrics analysis model for mapping the structural remodeling of the extracellular matrix in hypertrophic scars. The study finds that both fiber components become more disorganized in scar tissues, while content accumulation is observed from elastin fibers only. The 3D analysis can effectively distinguish normal and scar tissues and identify the boundaries. This imaging and analysis system uncovers the 3D architecture of the extracellular matrix in hypertrophic scars and has the potential for evaluating scars and identifying individualized treatment targets.
Article
Materials Science, Multidisciplinary
Christian Jonin, Estelle Salmon, Zacharie Behel, Faheem Ahmed, Mohammed Benali Kanoun, Chawki Awada, Pierre-Francois Brevet
Summary: In this study, second harmonic generation (SHG) and multiphoton excited photoluminescence (MEPL) were observed in retro-reflection mode from a 22 nm diameter anatase TiO2 nanoparticle powder. The relative contribution of SHG and MEPL depends on the conditions of photo-excitation, particularly the fundamental wavelength and the focal point depth location. Depth intensity profiles of SHG and MEPL differ significantly, with SHG profiles being narrower than that of MEPL.
Article
Dermatology
Mercedes Sendin-Martin, Jasmine Posner, Ucalene Harris, Matthew Moronta, Julian Conejo-Mir Sanchez, Sushmita Mukherjee, Milind Rajadhyaksha, Kivanc Kose, Manu Jain
Summary: Basal cell carcinoma (BCC) is the most common skin cancer, and non-invasive in vivo technologies such as multiphoton microscopy (MPM) can aid in diagnosing and prognosticating these tumors. This study explored the potential of MPM to differentiate collagen changes associated with different BCC subtypes compared to normal skin structures and benign lesions. The results showed that collagen distribution is more aligned surrounding BCCs nests compared to normal structures and benign lesions, and collagen is orientated more parallelly surrounding indolent BCC subtypes versus those with aggressive behavior.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Oncology
Danielle E. Desa, Wencheng Wu, Robert M. Brown, Edward B. Brown, Robert L. Hill, Bradley M. Turner, Edward B. Brownn
Summary: This study assessed the impact of neoadjuvant chemotherapy on tumor collagen in breast cancer patients. The results showed that chemotherapy affects tumor extracellular collagen in a subtype-specific manner and alters some prognostic signatures. This may have implications for the clinical utility of these signatures.
Article
Multidisciplinary Sciences
Rabindra Biswas, Medha Dandu, Asish Prosad, Sarthak Das, Sruti Menon, Jayanta Deka, Kausik Majumdar, Varun Raghunathan
Summary: Strong second-harmonic generation (SHG) has been observed from the 2H polytype of multilayer SnSe2 in the absence of excitonic resonances, with significant differences in SHG and Raman spectra between different polytypes and thickness-dependent behaviors. The enhanced nonlinear optical response at 1040 nm excitation is attributed to the near band-edge enhancement of nonlinear processes. The comparison of SHG from SnSe2 and MoS2 highlights the importance of considering the optical properties of 2D materials in nonlinear optical applications based on the wavelength range of interest.
SCIENTIFIC REPORTS
(2021)
Article
Optics
Hongsen He, Meng Zhou, Tian Qiao, Hei Ming Lai, Qiao Ran, Yu-Xuan Ren, Ho Ko, Chaogu Zheng, Kevin K. Tsia, Kenneth K. Y. Wong
Summary: In this study, we demonstrate a second-harmonic generation (SHG) microscopy using a frequency-doubled all-fiber mode-locked laser. The laser system operates at a similar to 890 nm light frequency and is achieved by controlling the emission peak of the gain fiber and using dispersion management technique. The SHG imaging performance is showcased on mouse samples and C. elegans, demonstrating its compact and efficient characteristics.
Article
Chemistry, Multidisciplinary
Shen Tong, Jincheng Zhong, Xinlin Chen, Xiangquan Deng, Jie Huang, Yingxian Zhang, Mengyuan Qin, Zhenhui Li, Hui Cheng, Wanjian Zhang, Lei Zheng, Weixin Xie, Ping Qiu, Ke Wang
Summary: Multiphoton microscopy (MPM) is a powerful technique for visualizing deep-brain structures at high resolution. This study demonstrates the measurement technology for the multiphoton excitation and emission properties of fluorescent labels at the 2200 nm window, and successfully applies it to deep-brain vasculature imaging in vivo.
Article
Optics
Shichao Zhang, Xingxin Huang, Deyong Kang, Jikui Miao, Zhenlin Zhan, Guoxian Guan, Jianxin Chen, Yongjian Zhou, Lianhuang Li
Summary: In this study, the feasibility of using SHG imaging to detect GISTs by monitoring the morphological changes of collagen fibers in the tumor microenvironment was assessed. The study found that collagen alterations occurred significantly in GISTs compared to normal tissues, and two morphological features from SHG images were extracted for quantitative assessment of the morphological difference of collagen fibers between normal muscular layer and GISTs through automated image analysis.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2023)
Article
Orthopedics
A. N. Jambor, E. M. Shelton, R. Kijowski, C. R. Henak, P. J. Campagnola
Summary: The study effectively demonstrated the disorganized collagen structure resulting from trypsin and collagenase degradation in cartilage, with collagenase showing the most significant effect. Application of a LD model successfully classified control and degraded tissues, indicating distinct activity of these enzymes on collagen assembly. Results from human cartilage suggested that collagenase effects were more representative of in vivo degeneration, emphasizing the potential of using high-resolution SHG and optical scattering as an earlier diagnostic tool for OA.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Engineering, Biomedical
Samuel Halvorsen, Ruizhi Wang, Yanhang Zhang
Summary: This study investigates the structure and mechanics of the ligamentum nuchae, particularly focusing on the contributions of elastin and collagen fibers to the tissue's nonlinear stress-strain behavior. The findings suggest that the intact tissue exhibits a significant stiffening behavior due to collagen engagement, while purified elastin tissue follows a similar stress-stretch response initially. The study also develops a transversely isotropic constitutive model to describe the mechanical behavior of both intact and purified elastin tissue. These findings provide insights into the roles of elastin and collagen fibers in tissue mechanics and have potential implications for tissue grafting using ligamentum nuchae.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Medicine, Research & Experimental
Xingxin Huang, Yuan-E Lian, Lida Qiu, XunBin Yu, Jikui Miao, Shichao Zhang, Zheng Zhang, Xiong Zhang, Jianxin Chen, Yannan Bai, Lianhuang Li
Summary: Nonalcoholic fatty liver disease is a common cause of chronic liver disease worldwide. This study used multiphoton microscopy to visualize important pathological features of nonalcoholic fatty liver disease and developed image analysis algorithms for quantifying disease progression. These findings can contribute to a better understanding of the disease and potential therapeutic targets.
LABORATORY INVESTIGATION
(2023)
Article
Optics
Alexander W. A. Murphy, Zichen Liu, Andrey V. Gorbach, Adelina Ilie, Ventsislav K. Valev
Summary: The study presents the first hyperspectral multiphoton analysis of densely-packed stacks of twisted and/or fused WS2 nanosheets, known as nanomesh, which exhibits scalable, efficient, and broadband optical properties due to double resonances induced by internal defects. This makes the nanomesh a prime candidate for integration into quantum optical technologies.
LASER & PHOTONICS REVIEWS
(2021)
Article
Biochemical Research Methods
Ekaterina Gubarkova, Vadim V. Elagin, Varvara V. Dudenkova, Sergey S. Kuznetsov, Maria M. Karabut, Arseny L. Potapov, Dmitry A. Vorontsov, Alexey Y. Vorontsov, Marina A. Sirotkina, Elena Zagaynova, Natalia D. Gladkova
Summary: This study showed the potential of multiphoton tomography using SHG and TPEF for visualizing different subtypes of human breast cancer, with a focus on assessing collagen fibers in low- and highly-aggressive cancers. TPEF detection of elastin fibers and amyloid protein may serve as a biomarker for low-aggressive breast cancer subtype detection, making this imaging technique a promising tool for rapid diagnosis and intraoperative assessment of tumor margins.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Instruments & Instrumentation
Yi Zhou, Deyong Kang, Zhenrong Yang, Lianhuang Li, Shuangmu Zhuo, Xiaoqin Zhu, Yongjian Zhou, Jianxin Chen
Article
Engineering, Electrical & Electronic
Lianhuang Li, Zhifen Chen, Xingfu Wang, Xing Liu, Weizhong Jiang, Shuangmu Zhuo, Guoxian Guan, Jianxin Chen
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2016)
Article
Optics
Lianhuang Li, Zhifen Chen, Deyong Kang, Tongxin Deng, Liwei Jiang, Yi Zhou, Xing Liu, Weizhong Jiang, Shuangmu Zhuo, Guoxian Guan, Pan Chi, Jianxin Chen
LASER PHYSICS LETTERS
(2016)
Article
Optics
Shu Wang, Liwei Jiang, Huiping Du, Xingfu Wang, Liqin Zheng, Lianhuang Li, Shuangmu Zhuo, Xiaoqin Zhu, Jianxin Chen
LASER PHYSICS LETTERS
(2016)
Review
Chemistry, Multidisciplinary
Hugh H. Chan, Connor A. Wathen, Ming Ni, Shuangmu Zhuo
Article
Biochemical Research Methods
Hongxin Lin, Chao Wei, Guangxing Wang, Hu Chen, Lisheng Lin, Ming Ni, Jianxin Chen, Shuangmu Zhuo
JOURNAL OF BIOPHOTONICS
(2019)
Article
Physics, Applied
Hongxin Lin, Chao Wei, Ning Cao, Hu Chen, Guangxing Wang, Jianxin Chen, Guannan Chen, Shuangmu Zhuo
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2019)
Article
Surgery
Xiaoling Zheng, Ning Zuo, Hongxin Lin, Liqin Zheng, Ming Ni, Guizhu Wu, Jianxin Chen, Shuangmu Zhuo
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2020)
Article
Biochemical Research Methods
Guangxing Wang, Yang Sun, Youting Chen, Qiqi Gao, Dongqing Peng, Hongxin Lin, Zhenlin Zhan, Zhiyi Liu, Shuangmu Zhuo
JOURNAL OF BIOPHOTONICS
(2020)
Article
Physics, Applied
Xueqin Xu, Guangxing Wang, Dongqing Peng, Liqin Zheng, Lisheng Lin, Xiaoling Zheng, Zhenlin Zhan, Shuangmu Zhuo
Summary: This study used nonlinear optical microscopy (NLOM) to differentiate between normal gastric mucosae and early gastric cancer, exploring the quantitative relationship between submucosal infiltration depth and collagen signals. Significant differences in collagen signals at different infiltration depths for early gastric cancer were observed, highlighting the potential of NLOM for real-time in situ diagnosis of early gastric cancer and accurate individualized treatment guidance.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Engineering, Electrical & Electronic
Huiling Zhan, Caihong Sun, Tianyi Luo, Gangqin Xi, Yikun Guo, Mingyu Xu, Youting Chen, Shuangmu Zhuo
Summary: This study uses multiphoton microscopy (MPM) to image the boundaries of hilar cholangiocarcinoma (HCC) tumors. The experimental results show that MPM can clearly reveal the existence of residual cancer cells at the surgical margin. By quantitatively calculating the density and direction of collagen fibers, it is possible to determine whether the resection margin is negative, which is closely related to the prognosis of HCC.
IEEE PHOTONICS JOURNAL
(2022)
Article
Multidisciplinary Sciences
Shenyi Jiang, Shuhao Qian, Lingxi Zhou, Jia Meng, Rushan Jiang, Chuncheng Wang, Xinguo Fang, Chen Yang, Zhihua Ding, Shuangmu Zhuo, Zhiyi Liu
Summary: The study uses label-free multiphoton microscopy to investigate the collagen and elastin fibers in human skin specimens and proposes a multi-fiber metrics analysis model for mapping the structural remodeling of the extracellular matrix in hypertrophic scars. The study finds that both fiber components become more disorganized in scar tissues, while content accumulation is observed from elastin fibers only. The 3D analysis can effectively distinguish normal and scar tissues and identify the boundaries. This imaging and analysis system uncovers the 3D architecture of the extracellular matrix in hypertrophic scars and has the potential for evaluating scars and identifying individualized treatment targets.
Article
Engineering, Electrical & Electronic
Guangxing Wang, Huiling Zhan, Tianyi Luo, Bingzi Kang, Xiaolu Li, Gangqin Xi, Zhiyi Liu, Shuangmu Zhuo
Summary: Surgery is a crucial method for treating ovarian cancer, but conventional biopsies for intraoperative histopathological diagnosis are labor-intensive, time-consuming, and prone to bias. Therefore, we propose a novel optical biopsy method using deep learning techniques to assist physicians in accurately and rapidly diagnosing ovarian cancer during surgery.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Hongxin Lin, Jianlei Xie, Taojian Fan, Youwu He, Jianxin Chen, Han Zhang, Shuangmu Zhuo
Review
Chemistry, Multidisciplinary
Ming Ni, Shuangmu Zhuo
