Article
Engineering, Biomedical
Xianhao Dong, Siyang Liu, Yueyue Yang, Shan Gao, Wenlei Li, Jiasong Cao, Ye Wan, Ziqi Huang, Guanwei Fan, Quan Chen, Hongjun Wang, Meifeng Zhu, Deling Kong
Summary: Research on nerve conduits composed of oriented microfiber-bundle cores and randomly organized nanofiber sheaths has shed light on the regulatory role of microfiber orientation in promoting peripheral nerve regeneration, elucidating a cascade of biological responses and ultimately enhancing nerve regeneration outcomes.
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Engineering, Biomedical
Papon Muangsanit, Victoria Roberton, Eleni Costa, James B. Phillips
Summary: Vascularisation is crucial for nerve tissue engineering to support long-term survival of implanted cells. Constructs containing endothelial cells have shown to promote axonal regeneration and vascularisation across peripheral nerve gaps.
ACTA BIOMATERIALIA
(2021)
Review
Biochemistry & Molecular Biology
Andrea Lavorato, Stefania Raimondo, Marina Boido, Luisa Muratori, Giorgia Durante, Fabio Cofano, Francesca Vincitorio, Salvatore Petrone, Paolo Titolo, Fulvio Tartara, Alessandro Vercelli, Diego Garbossa
Summary: The study discusses the features and applications of different types of mesenchymal stem cells in peripheral nerve regeneration after nerve injury. ADSCs are considered the most suitable MSCs for recovery from peripheral nerve lesions due to their excellent support and stimulation of axonal growth, remarkable paracrine activity, differentiation potential, low immunogenicity, and high survival rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Sean Mccracken, Michael J. Fitzpatrick, Allison L. Hall, Zelun Wang, Daniel Kerschensteiner, Josh L. Morgan, Philip R. Williams
Summary: Retinal ganglion cell (RGC) degeneration, caused by axon degeneration, is the main reason for vision loss in blinding conditions. Axonal Ca2+ elevations from optic nerve injury do not reach RGC somas, and baseline Ca2+ levels of RGCs predict their survival after axon injury.
Article
Nanoscience & Nanotechnology
Wen Xue, Yunfan Kong, Rafay Abu, Pooja Roy, Sung-Ho Huh, Mitchell Kuss, Vikas Kumar, Bin Duan
Summary: Decellularized nerve hydrogels (dNHs) have shown promising effects on promoting Schwann cell proliferation and neurite outgrowth. The mechanics and components of the hydrogel contribute to its functionality, with laminin (LAM) and type V collagen (COLV) playing important roles. Additionally, by adding LAM and COLV to collagen hydrogels, gene expression of Schwann cells can be regulated, leading to improved spreading and neurite outgrowth. Furthermore, dNHs can be fabricated into paper-like, aligned nerve scaffolds for peripheral nerve injury treatment.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biotechnology & Applied Microbiology
Kailei Xu, Xixia Liu, Xiaokeng Li, Jun Yin, Peng Wei, Jin Qian, Jie Sun
Summary: The study demonstrated that electromechanical stimulation affects the proliferation and axon outgrowth of nerve cells, with mechanical stretching reducing these effects. Cellular axons prefer to align perpendicularly to the stretch direction, showing a dependence on stretching direction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Luca Scaccini, Roberta Mezzena, Alessia De Masi, Mariacristina Gagliardi, Giovanna Gambarotta, Marco Cecchini, Ilaria Tonazzini
Summary: The study presents a method using chitosan scaffolds to promote nerve regeneration, showing that asymmetric directional topographies can enhance cell proliferation and migration speed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Fangwei Qi, Xiaosong Chen, Huixing Li, Gang Chen, Xinrui Luo, Shiyu Xiong, Shuping Peng, Cijun Shuai
Summary: Bismuth sulfide (Bi2S3) can convert light signals into electrical signals via the photoelectric effect, but the rapid recombination of electron-hole pairs weakens its photocurrent. To address this issue, a Bi2S3/Ti3C2Tx heterojunction was synthesized and mixed with PLLA to fabricate a Bi2S3/Ti3C2Tx-PLLA conduit. The heterojunction improves electron-hole pair separation and electron transfer, leading to an increased photocurrent and enhanced differentiation of PC12 into functional neurons by upregulating Ca2+ influx.
ACS APPLIED NANO MATERIALS
(2023)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yaxian Wang, Fuchao Zhang, Yunsong Zhang, Qi Shan, Wei Liu, Fengyuan Zhang, Feiyu Zhang, Sheng Yi
Summary: Research findings indicate that Btc plays essential roles in regulating Schwann cell migration and axon elongation, suggesting the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
MOLECULAR MEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Jue Ling, Chang He, Shuxuan Zhang, Yahong Zhao, Meifeng Zhu, Xiaoxuan Tang, Qiaoyuan Li, Liming Xu, Yumin Yang
Summary: This article provides an overview of evaluation methods used to study scaffold-based therapies for peripheral nerve injury (PNI) in experimental animal models, with particular focus on Schwann cell functions and axonal growth within the regenerated nerve.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Trevor Poitras, Douglas W. Zochodne
Summary: Common mechanisms of peripheral axon regeneration are recruited following diverse forms of damage to peripheral nerve axons. Supporting peripheral axon regrowth could be achieved through direct axon support or manipulation of the inflammatory milieu. Understanding the molecular barriers to regrowth and exploiting the downstream signaling pathways that impact neuron growth are promising approaches for promoting adult peripheral axon regeneration. This review emphasizes intrinsic aspects of adult peripheral axon regeneration and highlights several molecular barriers to regrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Raju Poongodi, Ying-Lun Chen, Tao-Hsiang Yang, Ya-Hsien Huang, Kuender D. Yang, Hsin-Chieh Lin, Jen-Kun Cheng
Summary: Various natural polymers and protein polymers have been utilized to create bio-scaffolds for nerve repair, supporting in vitro cell adhesion, mechano-transduction, proliferation, and differentiation, as well as controlled degradation and re-absorption for successful clinical outcomes. Recent advances in 3D bio-printing have also shown promise for nerve regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Liam A. McMorrow, Adrian Kosalko, Daniel Robinson, Alberto Saiani, Adam J. Reid
Summary: Patients with major peripheral nerve injury have poor recovery outcomes due to slow axonal regeneration and deterioration in the ability of the distal stump to support axonal growth. Schwann cells play a crucial role in nerve regeneration, but chronic denervation leads to instability in their repair phenotype and hinders regeneration support. Recent research on the cellular machinery, particularly the role of c-Jun, has opened opportunities for therapeutic intervention to support prolonged neural regeneration.
Article
Medicine, Research & Experimental
Jennifer Kersigo, Lintao Gu, Linjing Xu, Ning Pan, Sarath Vijayakuma, Timothy Jones, Seiji B. Shibata, Bernd Fritzsch, Marlan R. Hansen
Summary: The expression of Neurod1 reduces the progression of slow-growing MB and decreases cell proliferation in primary human SC cultures. In a genetic mouse model of schwannomas, the effects of Neurod1 expression are present but the high variability indicates a need for more tightly regulated expression levels to validate the effects on schwannoma progression.
Article
Otorhinolaryngology
Heba Isaac, Andrew Liu, Seiji B. Shibata, Marlan R. Hansen
Summary: Petrous apicitis (PA) is a serious infection involving the apical portion of the petrous temporal bone, with the classic triad of symptoms rarely seen due to early detection and antibiotic use. This case report presented a successful treatment approach for PA using a combined transmastoid and middle cranial fossa (MCF) approach after multidisciplinary evaluation. The patient recovered well with no recurrence of infection.
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY
(2021)
Article
Audiology & Speech-Language Pathology
Nicholas Giuliani, Marlan R. Hansen
Summary: This case study found that for patients with well-preserved hearing and poor performance with a hybrid cochlear implant device, switching back to bilateral hearing aid use can result in better auditory outcomes.
INTERNATIONAL JOURNAL OF AUDIOLOGY
(2022)
Article
Otorhinolaryngology
Kristen L. Seligman, A. Eliot Shearer, Kathy Frees, Carla Nishimura, Diana Kolbe, Camille Dunn, Marlan R. Hansen, Bruce J. Gantz, Richard J. H. Smith
Summary: Comprehensive genetic testing was performed on patients undergoing cochlear implantation, with 28% testing positive for genetic causes of hearing loss. A total of 44 causative genes were identified, with pediatric patients having a higher diagnostic rate. This study sets the foundation for future research on the relationship between genetic variation and cochlear implant performance.
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2022)
Article
Neurosciences
Viral D. Tejani, Hyejin Yang, Jeong-Seo Kim, Helin Hernandez, Jacob J. Oleson, Marlan R. Hansen, Bruce J. Gantz, Paul J. Abbas, Carolyn J. Brown
Summary: Acoustic hearing can be preserved after cochlear implant surgery, but a delayed loss of residual hearing may occur in some patients, possibly due to increases in electrode impedances associated with intracochlear inflammation/fibrosis. Access resistance rather than polarization impedance appears to drive the increase in total impedances seen with loss of hearing. Studies suggest that intracochlear inflammation may contribute to loss of acoustic hearing in EAS CI users.
JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY
(2022)
Article
Medicine, Research & Experimental
Megan J. Jensen, Heba Isaac, Helin Hernandez, Jacob Oleson, Camille Dunn, Bruce J. Gantz, Marlan R. Hansen
Summary: Long-term low frequency hearing preservation is achievable for recipients of Cochlear implants, regardless of gender or device type. Older recipients may experience poorer initial hearing outcomes, but there is no significant difference in the rate of hearing loss over time between older and younger patients. Early hearing loss after implantation does not serve as an indicator for long-term hearing loss.
Article
Otorhinolaryngology
Allan M. Henslee, Christopher R. Kaufmann, Matt D. Andrick, Parker T. Reineke, Viral D. Tejani, Marlan R. Hansen
Summary: An ECochG-guided robotics-assisted CI insertion system was developed to detect real-time drops in ECochG signals during electrode array insertions and immediately alter the insertion motion. This system provides surgeons with a means to monitor and reduce CI insertion-related trauma, potentially improving CI hearing outcomes.
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2022)
Article
Clinical Neurology
Thomas Lenarz, Andreas Buechner, Bruce Gantz, Marlan Hansen, Viral D. Tejani, Robert Labadie, Brendan O'Connell, Craig Alan Buchman, Carla V. Valenzuela, Oliver F. Adunka, Michael S. Harris, William J. Riggs, Douglas Fitzpatrick, Kanthaiah Koka
Summary: This study compared the intraoperative intra-cochlear electrocochleography (ECochG) with hearing preservation outcomes in cochlear implant (CI) subjects. The results showed a moderate positive correlation between the size of the ECochG drop and the magnitude of pure tone average change. This information can be useful for surgical decision-making regarding electrode insertion and hearing preservation.
OTOLOGY & NEUROTOLOGY
(2022)
Article
Audiology & Speech-Language Pathology
Alexander D. Claussen, Rene Vielman Quevedo, Jonathon R. Kirk, Timon Higgins, Brian Mostaert, Muhammad Taifur Rahman, Jacob Oleson, Reyna Hernandez, Keiko Hirose, Marlan R. Hansen
Summary: This study examined the contributions of surgical trauma, foreign body response, and electrical stimulation to intracochlear fibrosis and the innate immune response to cochlear implantation. The results showed that surgical trauma and the presence of a foreign body can induce an innate immune response and contribute to the development of intracochlear fibrosis.
Review
Audiology & Speech-Language Pathology
Bruce J. Gantz, Marlan Hansen, Camille C. Dunn
Summary: Preservation of residual acoustic hearing is an important concept for individuals with residual low frequency hearing undergoing cochlear implantation. Acoustic plus electric speech processing improves hearing outcomes in quiet, enables melody recognition, preserves spatial hearing if there is acoustic hearing in both ears, and significantly improves hearing in noise.
Review
Audiology & Speech-Language Pathology
Muhammad T. Rahman, Divya A. Chari, Gail Ishiyama, Ivan Lopez, Alicia M. Quesnel, Akira Ishiyama, Joseph B. Nadol, Marlan R. Hansen
Summary: Cochlear implants provide effective auditory rehabilitation for patients with severe to profound sensorineural hearing loss. This article reviews the impact of the inflammatory/foreign body response (FBR) on cochlear implants, the factors affecting the response, and therapeutic strategies to mitigate it. The understanding of the FBR has the potential to improve current cochlear implants and facilitate the development of neural prostheses.
Article
Audiology & Speech-Language Pathology
Megan J. Jensen, Alexander D. Claussen, Timon Higgins, Rene Vielman-Quevedo, Brian Mostaert, Linjing Xu, Jonathon Kirk, Marlan R. Hansen
Summary: This study assessed the effects of cochlear implant biomaterials on macrophages and fibroblasts, and their influence on fibrous tissue growth and new bone formation in the cochlea. The results showed that platinum substrates and contacts promoted cell adhesion, proliferation, and fibrosis compared to PDMS. Furthermore, platinum contacts influenced the deposition of new bone.
Article
Clinical Neurology
Alexander D. Claussen, Seiji B. Shibata, Christopher R. Kaufmann, Allan Henslee, Marlan R. Hansen
Summary: The study investigated the impact of robotics-assisted cochlear implant insertions on reducing intracochlear trauma. The results showed that the use of robotics-assisted system significantly decreased trauma scores compared with manual insertions, suggesting the potential for improved auditory outcomes in clinical trials.
OTOLOGY & NEUROTOLOGY
(2022)
Article
Medicine, Research & Experimental
Armine Kocharyan, Amanda M. Schaefer, Richard J. H. Smith, Marlan R. Hansen
Article
Audiology & Speech-Language Pathology
Osama Tarabichi, Tatiana Correa, Emre Kul, Stacia Phillips, Bahaa Darkazanly, Samuel M. Young Jr, Marlan R. Hansen
Summary: Viral vector gene therapy using Helper-dependent adenoviral vectors (HdAd) shows potential for treating hearing loss. HdAd vectors can express large or multiple genes in a cell-type specific manner. Two HdAd vectors, HdAd5 and a chimeric HdAd 5/35, were created and delivered into mice to evaluate their efficacy in inner ear gene therapy. Both HdAd vectors successfully transduced multiple cell types in the cochlea, suggesting their promise in treating hearing loss.
