Article
Plant Sciences
Xiyang Yang, Xingbing Li, Minghao Luo, Yongzheng Guo, Chang Li, Dingyi Lv, Zhe Cheng, Longxiang Huang, Fei-Fei Shang, Bi Huang, Jian Shen, Suxin Luo, Jianghong Yan
Summary: Tubeimoside I (TBM) promotes angiogenesis through the activation of the eNOS-VEGF signaling pathway, making it a potential novel therapeutic agent for ischemic diseases.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ivana Tolj, Ana Stupin, Ines Drenjancevic, Petar Susnjara, Leon Peric, Marko Stupin
Summary: This study aimed to investigate the specific role of nitric oxide (NO) in micro- and macrovascular response to a 7-day high-salt (HS) diet in healthy individuals. It found that the HS diet resulted in impaired NO-mediated endothelial vasodilation, with increased eNOS, decreased nNOS, and unchanged iNOS concentration and NO serum level. The study also failed to support the concept of non-osmotic sodium storage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jaqueline Pereira Moura Soares, Diego Assis Goncalves, Ricardo Xisto de Sousa, Margareth Gori Mouro, Elisa M. S. Higa, Leticia Paulino Sperandio, Carolina Moraes Vitoriano, Elisa Bachir Santa Rosa, Fernanda Oliveira dos Santos, Gustavo Nery de Queiroz, Roberta Sessa Stilhano Yamaguchi, Gustavo Pereira, Marcelo Yudi Icimoto, Fabiana Henriques Machado de Melo
Summary: Cutaneous melanoma is the most aggressive type of skin cancer, and it progresses through different stages. Reactive oxygen species modulate oncogenic signaling pathways in all stages of melanoma development. The altered concentration of tetrahydrobiopterin disrupts cellular redox homeostasis and contributes to melanoma progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Amir Asgari, Paul Jurasz
Summary: Megakaryocytes are important members of the hematopoietic system, responsible for regulating vascular homeostasis through platelets. The process of megakaryopoiesis generates mature megakaryocytes which release proplatelets into blood vessels. Nitric oxide (NO) plays a modulatory role in hematopoiesis and hemostasis. This review summarizes the effect of NO and its signaling on megakaryopoiesis and thrombopoiesis under physiological and pathophysiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Paul Guerby, Oriane Tasta, Audrey Swiader, Frederic Pont, Emmanuel Bujold, Olivier Parant, Christophe Vayssiere, Robert Salvayre, Anne Negre-Salvayre
Summary: Preeclampsia is a pregnancy disease exclusive to humans, characterized by gestational hypertension, proteinuria, oxidative stress, and imbalance between NO and ROS production, possibly due to placental abnormalities and oxidative stress.
Article
Hematology
Arvind K. Pandey, Markus Waldeck-Weiermair, Quinn S. Wells, Wusheng Xiao, Shambhu Yadav, Emrah Eroglu, Thomas Michel, Joseph Loscalzo
Summary: This study reveals that CD70 plays a regulatory role in endothelial NO and reactive oxygen species homeostasis and is associated with vascular phenotypes. CD70 knockdown leads to reduced NO levels and increased reactive oxygen species, which may be related to endothelial dysfunction and cardiovascular diseases, highlighting its significance in human vascular diseases.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Cell Biology
Livius d'Uscio, Zvonimir S. Katusic
Summary: The study revealed an age-related increase in APP expression in vascular endothelium, which is believed to be an adaptive response aimed at protecting and maintaining eNOS function. Endothelial dysfunction was exacerbated in aged eAPP(-/-) mice compared to age-matched WT mice, suggesting a crucial role of APP in controlling endothelial function during aging.
Article
Radiology, Nuclear Medicine & Medical Imaging
Chiao-Chi Chen, Chen Chang, Ming-Fang Lin, Guo-Shu Huang, Wing P. Chan
Summary: During acute ischemic stroke, the interaction between deoxyhemoglobin and nitric oxide can be detected using MRI, serving as a sensitive marker for assessing tissue oxygenation status in the ischemic region.
MAGNETIC RESONANCE IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Thuan Thai, Fei Zhong, Lei Dang, Enoch Chan, Jacqueline Ku, Ernst Malle, Carolyn L. Geczy, John F. Keaney, Shane R. Thomas
Summary: The study demonstrates that transcytosed MPO in endothelial cells activates eNOS by promoting phospholipase C-dependent calcium signaling and altering eNOS phosphorylation status. This activation occurs despite reduced cellular uptake of the eNOS substrate L-arginine. These findings suggest a compensatory signaling response in endothelial cells to maintain eNOS activity and NO production in the presence of MPO-induced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Kenichiro Suno, Yasushige Shingu, Satoru Wakasa
Summary: This study evaluated the effects of trehalose preconditioning on cardiac and coronary endothelial function after ischemia-reperfusion injury. It was found that trehalose preconditioning improved heart function, reduced edema, oxidative stress, and apoptosis, and increased eNOS dimerization ratio, indicating its protective effects on cardiac and coronary endothelial function. These effects were mediated through the eNOS signaling pathway.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Szabolcs Zahoran, Peter R. Szanto, Nikolett Bodi, Maria Bagyanszki, Jozsef Maleth, Peter Hegyi, Tamas Sari, Edit Hermesz
Summary: The study investigated the mechanisms of prenatal endothelial dysfunction induced by maternal smoking, revealing deactivation of endothelial nitric oxide synthase and up-regulation of compensatory pathways in UC vessels. Smoking-induced damage in vessels is proportional to exposure level, with increased formation of reactive oxygen species and up-regulation of highly efficient NO production pathway in primarily exposed veins.
Article
Pharmacology & Pharmacy
Tatsiana Suvorava, Sara Metry, Stephanie Pick, Georg Kojda
Summary: Vascular tone regulation by nitric oxide is a crucial physiological function, with endothelial dysfunction potentially being an underlying cause of hypertension. Increasing eNOS activity reduces blood pressure, while decreasing activity has the opposite effect. These findings have important implications for pre-hypertensive treatment.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Shupeng Hu, Qiangzhong Pi, Xiudan Xu, Jianghong Yan, Yongzheng Guo, Wanying Tan, An He, Zhe Cheng, Suxin Luo, Yong Xia
Summary: This study investigates the role of eNOS in vascular dysfunction induced by sepsis. Increased phosphorylation of eNOS in early sepsis leads to decreased vasodilation, while decreased levels of eNOS in late sepsis result in diminished nitric oxide-dependent relaxation responses.
Article
Biochemistry & Molecular Biology
Ming-Jie Hou, Pan Wang, Bao Ting Zhu
Summary: In this study, it was found that protein disulfide isomerase (PDI)-mediated neuronal nitric oxide synthase (nNOS) activation plays a critical role in chemically-induced ferroptosis, which is characterized by the accumulation of nitric oxide (NO), reactive oxygen species (ROS), and lipid-ROS. Inhibition or knockdown of PDI can effectively prevent ferroptotic cell death.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Biochemistry & Molecular Biology
Yuka Okajima, Takashi Matsuzaka, Shun Miyazaki, Kaori Motomura, Hiroshi Ohno, Rahul Sharma, Takuya Shimura, Nurani Istiqamah, Song-iee Han, Yuhei Mizunoe, Yoshinori Osaki, Hitoshi Iwasaki, Shigeru Yatoh, Hiroaki Suzuki, Hirohito Sone, Takafumi Miyamoto, Yuichi Aita, Yoshinori Takeuchi, Motohiro Sekiya, Naoya Yahagi, Yoshimi Nakagawa, Tsutomu Tomita, Hitoshi Shimano
Summary: The research found various alterations in pancreatic islet vasculature in obese insulin-resistant mice and type 2 diabetic mice, including capillary dilation, increased blood flow, and decreased endothelial nitric oxide synthase activity. Amelioration of these abnormalities can be achieved through Elovl6 deficiency.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)