Article
Cell Biology
Eya Hamza, Mercedes Vallejo-Mudarra, Hakim Ouled-Haddou, Cristina Garcia-Caballero, Melania Guerrero-Hue, Laure Santier, Sandra Rayego-Mateos, Islam Amine Larabi, Jean -Claude Alvarez, Loic Garcon, Ziad A. Massy, Gabriel Choukroun, Juan Antonio Moreno, Laurent Metzinger, Valerie Metzinger-Le Meuth
Summary: Chronic kidney disease (CKD) is a global health condition characterized by a progressive deterioration of kidney function. Uremic toxins, such as Indoxyl Sulfate (IS), are associated with CKD-related complications, including anemia. Our study investigated the impact of IS on erythropoiesis in CKD patients, and found that IS can induce apoptosis and hinder the differentiation of erythroid progenitor cells. Additionally, IS deregulated the expression of erythropoietic-related genes. These findings suggest that IS may contribute to the development of anemia in CKD.
CELLULAR SIGNALLING
(2023)
Review
Biochemistry & Molecular Biology
Chien-Lin Lu, Cai-Mei Zheng, Kuo-Cheng Lu, Min-Tser Liao, Kun-Lin Wu, Ming-Chieh Ma
Summary: The uremic toxin IS leads to oxidative stress in CKD patients, causing organ damage and complications such as cardiovascular diseases, renal osteodystrophy, muscle wasting, and anemia. IS-induced ROS production impairs glomerular filtration and triggers vascular smooth muscle cells transformation, contributing to muscle atrophy and bone abnormalities.
Article
Biochemistry & Molecular Biology
Li-Ting Tsai, Te- Weng, Ting-Yu Chang, Kuo-Cheng Lan, Chih-Kang Chiang, Shing-Hwa Liu
Summary: The accumulation of the uremic toxin indoxyl sulfate (IS) is a key pathological feature of chronic kidney disease (CKD). Exposure to IS can induce ferroptosis, characterized by iron accumulation, impaired antioxidant system, elevated reactive oxygen species (ROS) levels, and lipid peroxidation. IS triggers intracellular iron accumulation and ROS generation, leading to the induction of ferroptosis, senescence, ER stress, and injury/fibrosis in CKD kidneys.
Review
Biochemistry & Molecular Biology
Kuo-Chin Hung, Wei-Cheng Yao, Yi-Lien Liu, Hung-Jen Yang, Min-Tser Liao, Keong Chong, Ching-Hsiu Peng, Kuo-Cheng Lu
Summary: Patients with chronic kidney disease (CKD) often have a high accumulation of protein-bound uremic toxins (PBUTs), such as indoxyl sulfate (IS) and p-cresyl sulfate (pCS). The buildup of PBUTs inhibits bone and muscle function, worsens muscle wasting, and contributes to low bone turnover disorders. Understanding the underlying mechanisms of bone and muscle loss in CKD can aid in developing new therapies for musculoskeletal diseases.
Article
Medicine, General & Internal
Min Xiao, Ajay K. Medipally, Laura Biederman, Anjali A. Satoskar, Iouri Ivanov, Brad H. Rovin, Sergey V. Brodsky
Summary: Chronic hematuria results in increased iron-positive tubular epithelial cells, EMT, apoptosis, and more prominent IFTA progression in CKD rats. Our data suggest an important role of chronic hematuria in the progression of CKD.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
George Chang, Hong-Mou Shih, Chi-Feng Pan, Chih-Jen Wu, Cheng-Jui Lin
Summary: Studies have shown that a low-protein diet supplemented with ketoanalogs (KAs) can significantly slow down the progression of renal function in patients with stages 3-5 chronic kidney disease (CKD). However, it is unclear how it affects endothelial function and serum levels of protein-bound uremic toxins. This study aimed to investigate the effects of a low-protein diet supplemented with KAs on kidney function, endothelial function, and serum uremic toxin levels in a cohort of CKD patients. The results demonstrated that LPD supplemented with KAs significantly preserved kidney function and improved endothelial function and protein-bound uremic toxins in patients with CKD.
Article
Food Science & Technology
Su Mi Lee, Mi Yeun Han, Su Hyun Kim, Ran Hui Cha, Seock Hui Kang, Jun Chul Kim, Won Suk An
Summary: Serum myostatin and indoxyl sulfate levels are associated with sarcopenia in chronic kidney disease. Higher levels of indoxyl sulfate are associated with sarcopenia, while higher levels of myostatin are associated with muscle mass in patients with CKD.
Article
Food Science & Technology
Carolla El Chamieh, Islam Amine Larabi, Solene M. Laville, Christian Jacquelinet, Christian Combe, Denis Fouque, Maurice Laville, Luc Frimat, Roberto Pecoits-Filho, Celine Lange, Benedicte Stengel, Natalia Alencar De Pinho, Jean-Claude Alvarez, Ziad A. Massy, Sophie Liabeuf
Summary: Proton-pump inhibitors (PPIs) are commonly used in patients with chronic kidney disease (CKD), and are associated with the retention of uremic toxins (UTs). In this cross-sectional study, we found that patients using PPIs had higher levels of certain UTs compared to other patients. These findings are important for understanding factors that modulate serum UT concentration in CKD patients.
Article
Transplantation
Stanislas Bataille, Laetitia Dou, Marc Bartoli, Marion Sallee, Julien Aniort, Borhane Ferkak, Rania Chermiti, Nathalie McKay, Nathalie Da Silva, Stephane Burtey, Stephane Poitevin
Summary: In chronic kidney disease (CKD), the plasma concentrations of myostatin and activin A are increased, possibly due to reduced renal clearance. Furthermore, we observed increased production of activin A in the kidney and heart, which may be related to muscle wasting. Therefore, myostatin and activin A should be added to the list of uremic toxins.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Article
Pharmacology & Pharmacy
Dezhong Wang, Tianyang Zhao, Yushuo Zhao, Yuan Yin, Yuli Huang, Zizhao Cheng, Beibei Wang, Sidan Liu, Minling Pan, Difei Sun, Zengshou Wang, Guanghui Zhu
Summary: The FGF1 variant FGF1(Delta HBS) has been shown to inhibit podocyte EMT, depletion, renal fibrosis, and preserve renal function in two nephropathy models. Mechanistic studies revealed that the inhibitory effects of FGF1(Delta HBS) on podocyte EMT were mediated by decreased expression of transforming growth factor beta 1 via upregulation of PPAR gamma. These findings suggest potential therapeutic uses of FGF1(Delta HBS) in diseases associated with EMT.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Food Science & Technology
Kullaya Takkavatakarn, Pongpratch Puapatanakul, Jeerath Phannajit, Warumphon Sukkumme, Pajaree Chariyavilaskul, Patita Sitticharoenchai, Asada Leelahavanichkul, Pisut Katavetin, Kearkiat Praditpornsilpa, Somchai Eiam-Ong, Paweena Susantitaphong
Summary: Sevelamer therapy was more effective than calcium carbonate therapy in reducing the protein-bound uremic toxin p-cresyl sulfate. Sevelamer also helped lower fibroblast growth factor 23 and low-density lipoprotein cholesterol levels, showing benefits in terms of retarding CKD progression. No changes in vascular stiffness were found in this study.
Article
Geriatrics & Gerontology
Lara Caldiroli, Silvia Armelloni, Alessandra Eskander, Piergiorgio Messa, Vittoria Rizzo, Elisabetta Margiotta, Matteo Cesari, Simone Vettoretti
Summary: In a cross-sectional study of 93 patients with chronic kidney disease, it was found that indoxyl sulfate (IS) and p-cresyl sulfate (PCs) were not associated with sarcopenia, although they were linked to certain inflammatory pathways. Particularly, p-cresyl sulfate was positively associated with the Protein Energy Wasting syndrome (PEW).
EXPERIMENTAL GERONTOLOGY
(2021)
Article
Nutrition & Dietetics
Mirco Vacca, Giuseppe Celano, Francesco Maria Calabrese, Maria Teresa Rocchetti, Ilaria Iacobellis, Nadia Serale, Maria Calasso, Loreto Gesualdo, Maria De Angelis
Summary: This study evaluated the efficacy of an innovative synbiotic as an adjuvant therapy for IIIb-IV stage chronic kidney disease (CKD) patients. The synbiotic was found to significantly alter the microbiota profile and increase saccharolytic metabolism in feces. These findings suggest a selective effectiveness of the synbiotic for IIIb-IV stage CKD patients, but further validation with a larger patient population is necessary.
FRONTIERS IN NUTRITION
(2023)
Article
Multidisciplinary Sciences
Shuangdi Duan, Jiayi Pi, Chun-Hsiang Wang, Yi-Chou Hou, Chung-Ying Andy Lee, Cheng-Jui Lin, Liyang Shi, Kung-Chia Young, Hung-Yu Sun
Summary: This study validated the comparable efficacy of the Leadgene ELISA kit to UPLC-MS/MS in quantifying serum IS levels, showing a perfect correlation between the IS levels determined by the two methods in CKD patients.
Review
Nutrition & Dietetics
Li Chen, Junhe Shi, Xiaojuan Ma, Dazhuo Shi, Hua Qu
Summary: Microbiota-driven therapy has a modest effect on circulating IS concentration but can decrease PCS concentration in CKD patients. Prebiotic and synbiotic supplementation can effectively reduce PCS concentration, while probiotic supplementation does not show significant effects.
ADVANCES IN NUTRITION
(2022)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)