Article
Multidisciplinary Sciences
Md. Sadique Hasan, Chad Sundberg, Michael Tolosa, Abhay Andara, Xudong Ge, Yordan Kostov, Govind Rao
Summary: Rapid and accurate detection of bioburden is essential for various applications. We developed a microfluidic device with an integrated filter for trapping cells and detecting bioburden using a fluorescent label. The device was fabricated in a cost-effective and rapid manner, and showed significantly improved sensitivity compared to conventional methods. With a detection limit of 0.037 CFU/mL, this microfluidic device has the potential to be a portable tool for highly sensitive and high-throughput bacterial detection.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Fengtao Jiang, Nan Xiang
Summary: This paper presents the development of a novel handheld sorter with a multifunction integrated microfluidic chip for point-of-care diagnosis in resource-limited settings. The sorter utilizes a spiral inertial microfluidic channel and flow regulators for size-based cell separation. The prototype of the sorter successfully separates malignant tumor cells from clinical pleural effusions. The device is simple to use, inexpensive, portable, and compact, making it suitable for high-throughput label-free cell separation in resource-limited areas.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
June Soo Kim, Soon Yeol Kwon, Jae Yong Lee, Seung Deok Kim, Da Ye Kim, Hyunjun Kim, Noah Jang, Jiajie Wang, Maeum Han, Seong Ho Kong
Summary: A microfluidic resistive pulse sensing technique is presented for detecting and analysing microparticles, with challenges in noise and low throughput. The study introduces a microfluidic chip with multiple detection gates to improve throughput while maintaining simplicity. The proposed chip uses hydrodynamic sheathless particle focusing to detect resistive pulses, with a reference gate to minimize noise. It achieves high sensitivity in analysing physical properties of particles and exosomes, offering potential for exosome detection in biological and clinical applications.
Article
Chemistry, Multidisciplinary
Zihan Yang, Zhihang Zhou, Tongxu Si, Zhengdong Zhou, Li Zhou, Y. Rebecca Chin, Liang Zhang, Xinyuan Guan, Mengsu Yang
Summary: Cancer metastasis is a major cause of cancer-related death, and it is characterized by excessive extracellular matrix deposition and increased stiffness in solid tumors. The confined migration of tumor cells in these conditions plays a crucial role in metastasis, but inhibitors specifically targeting this type of migration are rare. In this study, a microfluidic chip was designed to screen for drugs that can effectively inhibit confined migration of cancer cells. By applying this chip, three novel inhibitors of confined migration, MA-5, SB-705498, and diphenyleneiodonium chloride, were discovered in multiple cancer types. The mechanisms of action of these inhibitors were found to be targeting mitochondria, actin polymerization, and cell viability, respectively. Overall, a high-throughput microfluidic platform for screening drugs targeting confined migration was established, and three effective inhibitors were identified.
Article
Chemistry, Analytical
Seunghee Choo, Hyunjung Lim, Tae Eun Kim, Jion Park, Kyu Been Park, Chaewon Park, Chae Seung Lim, Jeonghun Nam
Summary: Water contamination is a critical global public health issue. This study presents a viscoelastic microfluidic device for the continuous concentration of bacterial cells, aiming to address the limitations of current detection methods. The device was validated for cell concentration by optimizing flow characteristics and achieving highly concentrated bacterial cells.
Article
Biochemical Research Methods
Roberto Rodriguez-Moncayo, Diana F. Cedillo-Alcantar, Pablo E. Guevara-Pantoja, Oriana G. Chavez-Pineda, Jose A. Hernandez-Ortiz, Josue U. Amador-Hernandez, Gustavo Rojas-Velasco, Fausto Sanchez-Munoz, Daniel Manzur-Sandoval, Luis D. Patino-Lopez, Daniel A. May-Arrioja, Rosalinda Posadas-Sanchez, Gilberto Vargas-Alarcon, Jose L. Garcia-Cordero
Summary: The applications of serology tests for SARS-CoV-2 include diagnosing COVID-19, understanding humoral responses, estimating prevalence, and modeling the pandemic. A new high-throughput microfluidic device can assess antibody reactivities to four SARS-CoV-2 antigens from 50 serum samples simultaneously, with high sensitivity and specificity.
Article
Chemistry, Analytical
Bryan Ang, Ruhollah Habibi, Ciaren Kett, Wai Hoe Chin, Kellie L. Tuck, Adrian Neild, Victor J. Cadarso
Summary: Microfluidic concentration technology can detect low concentrations of bacteria in samples, making it suitable for time-sensitive situations such as clinical settings and food quality control. By using a packed bed of micro-particles activated by surface acoustic waves, bacteria can be efficiently captured and recovered for further analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Priyatanu Roy, Margaret L. House, Cari S. Dutcher
Summary: This study presented a temperature-controlled microfluidic platform for high-throughput ice nucleation studies in droplets, with automated droplet freezing detection methods using a deep neural network and polarized optical method. A case study on Snomax(R) revealed the impact of aging and heat treatment on ice nucleation ability, with the platform generating droplets at a rate of about 1500/min and minimal human intervention needed for classification by DNN.
Review
Chemistry, Analytical
Jinpei Wang, Lixia Yang, Hanghui Wang, Lin Wang
Summary: Microfluidic chips play a significant role in the detection of airborne microorganisms. They are capable of detecting and analyzing bacteria, viruses, and fungi with high sensitivity and potential, including Escherichia coli, Staphylococcus aureus, H1N1 virus, SARS-CoV-2 virus, and Aspergillus niger.
Article
Chemistry, Analytical
Zhenqing Li, Yifei Wang, Zehang Gao, Shinichi Sekine, Qingxiang You, Songlin Zhuang, Dawei Zhang, Shilun Feng, Yoshinori Yamaguchi
Summary: We proposed a double-layer droplet CF-PCR microfluidic chip to overcome the drawbacks of CF-PCR, such as high resistance and evaporation. Experimental results showed that the target genes of Porphyromonas gingivalis were successfully amplified within a short period of time, providing a new approach for rapid bacterial detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Yuhe Wang, Wuzhen Qi, Lei Wang, Jianhan Lin, Yuanjie Liu
Summary: A new magnetic extraction method combined with microfluidic PCR was developed for detecting Salmonella, with an extraction efficiency of over 90% and a detection limit of 10(2) CFU/mL, promising rapid, sensitive, and simultaneous detection of multiple foodborne pathogens.
Article
Chemistry, Multidisciplinary
Bo Zhang, Ansila Mathoor, Tanja Junkers
Summary: Describes an automated high-throughput multidimensional reaction screening platform based on an inline Fourier-transform infrared spectroscopy. The platform combines flow chemistry, machine automation, and inline analysis to rapidly and efficiently screen reactions in multiple dimensions. It enables the construction of kinetic data libraries with high precision, reproducibility, and data density. The method allows for precise prediction of reaction outcomes within the high-dimensional chemical parameter space that is screened, and is a key step in providing big data for data-driven research.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Natalie J. Wu-Woods, Jacob T. Barlow, Florian Trigodet, Dustin G. Shaw, Anna E. Romano, Bana Jabri, A. Murat Eren, Rustem F. Ismagilov
Summary: This study introduces a microbial-enrichment method (MEM) that enables the removal of host DNA in human intestinal biopsies and allows for the characterization of low-abundance microbial taxa. The method provides deeper insights into the high-throughput characterization of the gut microbiome and demonstrates the construction of metagenome-assembled genomes from human intestinal biopsies.
Article
Biochemical Research Methods
Youngtaek Kim, Jiyoung Song, Younggyun Lee, Sunghyun Cho, Suryong Kim, Seung-Ryeol Lee, Seonghyuk Park, Yongdae Shin, Noo Li Jeon
Summary: The newly designed injection molded microfluidic device addresses the inherent limitations of PDMS-manufactured devices and provides a more convenient way to study single-cell behaviors while saving time. The spatiotemporal dynamics in the device were demonstrated through experiments and computational simulations. This easy-to-use plastic microfluidic device promises to open a new approach for investigating the mechanisms of cell behavior at the single-cell level.
Article
Veterinary Sciences
Nicole B. Goecke, Bodil H. Nielsen, Mette B. Petersen, Lars E. Larsen
Summary: Respiratory and enteric diseases in cattle have a significant impact on their health and productivity. A new high-throughput rtPCR system has been developed to detect multiple pathogens simultaneously, reducing costs and turnaround time. The system allows for testing of multiple samples for different pathogens in the same analysis test.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Fenni Zhang, Wenwen Jing, Ashley Hunt, Hui Yu, Yunze Yang, Shaopeng Wang, Hong-Yuan Chen, Nongjian Tao
Article
Chemistry, Analytical
Hui Yu, Wenwen Jing, Rafael Iriya, Yunze Yang, Karan Syal, Manni Mo, Thomas E. Grys, Shelley E. Haydel, Shaopeng Wang, Nongjian Tao
ANALYTICAL CHEMISTRY
(2018)
Correction
Chemistry, Analytical
Hui Yu, Wenwen Jing, Rafael Iriya, Yunze Yang, Karan Syal, Manni Mo, Thomas E. Grys, Shelley E. Haydel, Shaopeng Wang, Nongjian Tao
ANALYTICAL CHEMISTRY
(2018)
Article
Environmental Sciences
Wenwen Jing, Qi Liu, Mingyi Wang, Xinlian Zhang, Jianmin Chen, Guodong Sui, Lin Wang
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2019)
Article
Chemistry, Multidisciplinary
Wenwen Jing, Yan Wang, Yunze Yang, Yi Wang, Guangzhong Ma, Shaopeng Wang, Nongjian Tao
Article
Chemistry, Analytical
Manni Mo, Yunze Yang, Fenni Zhang, Wenwen Jing, Rafael Iriya, John Popovich, Shaopeng Wang, Thomas Grys, Shelley E. Haydel, Nongjian Tao
ANALYTICAL CHEMISTRY
(2019)
Article
Engineering, Electrical & Electronic
Rafael Iriya, Wenwen Jing, Karan Syal, Manni Mo, Chao Chen, Hui Yu, Shelley E. Hayde, Shaopeng Wang, Nongjian Tao
IEEE SENSORS JOURNAL
(2020)
Article
Chemistry, Multidisciplinary
Yi Wang, Yunze Yang, Chao Chen, Shaopeng Wang, Hui Wang, Wenwen Jing, Nongjian Tao
Article
Chemistry, Multidisciplinary
Runli Liang, Guangzhong Ma, Wenwen Jing, Yan Wang, Yunze Yang, Nongjian Tao, Shaopeng Wang
Summary: A charge-sensitive optical detection (CSOD) technique has been developed to detect molecular binding kinetics in normal ionic strength buffers, overcoming the challenge of decreased sensitivity in mass-based detection technologies for small molecules. By designing an H-shaped sample well and using agarose gels to cover electrodes, the study successfully observed stronger affinities between G-protein-coupled receptors and their small molecule ligands in normal buffer compared to diluted buffer, potentially due to stronger electrostatic repulsion forces in diluted buffer.
Article
Biochemistry & Molecular Biology
Wenwen Jing, Ashley Hunt, Nongjian Tao, Fenni Zhan, Shaopeng Wang
Article
Chemistry, Analytical
Yuhan Yao, Na Zhao, Wenwen Jing, Qi Liu, Huijun Lu, Wei Zhao, Wang Zhao, Zhiming Yuan, Han Xia, Guodong Sui
Summary: The newly developed self-powered rapid loading microfluidic chip can detect eight vector-borne viruses, offering fast operation, low cost, and accuracy, suitable for point-of-care diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Fenni Zhang, Jiapei Jiang, Michelle McBride, Yunze Yang, Manni Mo, Rafael Iriya, Joseph Peterman, Wenwen Jing, Thomas Grys, Shelley E. Haydel, Nongjian Tao, Shaopeng Wang
Article
Chemistry, Multidisciplinary
Yi Wang, Wenwen Jing, Nongjian Tao, Hui Wang
Summary: The study introduces an optical imaging technique for directly counting and monitoring binding and motion events of single nanoparticles with high sensitivity and temporal resolution, providing a new approach for studying individual dynamic behaviors.
Article
Chemistry, Analytical
Lin Li, Qingde Chen, Guodong Sui, Jing Qian, Chi-Tay Tsai, Xunjia Cheng, Wenwen Jing
Summary: A three-dimensional micromixer was developed based on splitting-recombination and chaotic advection principles, showing high efficiency in mixing. The complete mixing length varies depending on the Reynolds number, with different mechanisms dominating below and above a critical Reynolds number. Both numerical simulations and experimental results supported the efficiency of the micromixer design across a range of Reynolds numbers.
Article
Environmental Sciences
Chengli Zhang, Yuchen Lei, Jing Qian, Yixin Qiao, Jingchao Liu, Shuifeng Li, Lingyu Dai, Kexin Sun, Huimin Guo, Guodong Sui, Wenwen Jing
Summary: The study found that microplastics have a good adsorption effect on OCPs in soil, with equilibrium adsorption taking 12 hours and the process described by a pseudo-second-order model. The Freundlich model is more suitable for describing the adsorption isotherm data, indicating that the process occurs on the non-uniform surface of microplastics. Additionally, microplastics showed better adsorption effects on OCPs when the solution/soil ratio was between 75:1 and 100:1, and as the diameter of microplastics increased, the adsorption capacity decreased.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
