Article
Chemistry, Physical
Jiantao Feng, Quanmei Sun, Peipei Chen, Keli Ren, Yuanyuan Zhang, Yahong Shi, Songkun Gao, Zhiwei Song, Jigang Wang, Fulong Liao, Dong Han
Summary: Active deformation behavior is important for understanding cancer cell mechanics. A method using atomic force microscopy and deformation behavior cytometry is proposed to measure cancer cell deformation behavior. Aquaporins and the actin cytoskeleton play essential roles in promoting deformation behavior.
Article
Multidisciplinary Sciences
Qiang Zheng, Mingzeng Peng, Zhuo Liu, Shuyu Li, Rongcheng Han, Han Ouyang, Yubo Fan, Caofeng Pan, Weiguo Hu, Junyi Zhai, Zhou Li, Zhong Lin Wang
Summary: This study developed a force mapping method based on a light nano-antenna array with the use of piezo-phototronic effect, achieving high-resolution dynamic mapping of cell-generated force. The methodology can be applied for fundamental study of cardiomyocyte behavior and provide valuable information for heart-related research.
Article
Multidisciplinary Sciences
Saradha Venkatachalapathy, Doorgesh S. Jokhun, Madhavi Andhari, G. Shivashankar
Summary: Tumour progression is associated with complex biomechanical changes in the tissue microenvironment. This study demonstrates the importance of single cell chromatin and nuclear features as biomarkers for mapping tumor progression phenotypes. The spatial chromatin organization in individual nuclei can characterize cell states and their alterations during tumor progression.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Bashar Hamza, Alex B. Miller, Lara Meier, Max Stockslager, Sheng Rong Ng, Emily M. King, Lin Lin, Kelsey L. DeGouveia, Nolawit Mulugeta, Nicholas L. Calistri, Haley Strouf, Christina Bray, Felicia Rodriguez, William A. Freed-Pastor, Christopher R. Chin, Grissel C. Jaramillo, Megan L. Burger, Robert A. Weinberg, Alex K. Shalek, Tyler Jacks, Scott R. Manalis
Summary: This study presents a new method for measuring endogenous CTC kinetics by continuously exchanging blood between tumor-bearing and tumor-free mice. The research found varying half-life times of CTCs in circulation in different cancer models, and demonstrated the ability to generate distant metastases by transferring only 1-2% of shed CTCs. This technique is expected to further elucidate the role of CTCs and the rate-limiting steps in metastasis.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Kenji Nishizawa, Shao-Zhen Lin, Claire Chardes, Jean-Francois Rupprecht, Pierre-Francois Lenne
Summary: Biological tissues acquire reproducible shapes through dynamic cell behaviors, particularly the remodeling of cell-cell contacts. This study developed a technique to manipulate stress patterns on these contacts in the early epithelium of Drosophila embryos and found that Myosin -II activity responds to junction strain rate, facilitating junction shrinkage. These findings provide insights into the efficient deformation of cell-cell contacts in vivo and reveal the mechanosensitive features of their remodeling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biotechnology & Applied Microbiology
Ling An, Fenglong Ji, Enming Zhao, Yi Liu, Yaling Liu
Summary: Microfluidics is a popular method for studying cell deformation, with applications in cell biology, biophysics, and medical research. This review summarizes recent advances in microfluidic techniques for measuring cellular deformation and highlights their applications. Microfluidic chips provide a powerful platform for studying cell deformation and future developments are expected to improve their applications in biomedical research.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Shu-Ting Xu, Cheng Yang, Xiu-Ping Yan
Summary: The study introduces an all-in-one system for rapid single-HeLa-cell screening and in-depth profiling of cellular metabolites and drugs. Utilizing zinc oxide nanothorn-decorated filters for efficient online lysis of cell membrane, the system showed promising results for subcellular discrimination and signal response improvements. This system has the potential for high-coverage single-cell metabolome analysis in cell biology and cancer research.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Biomedical
Stephen Coyle, Bryant Doss, Yucheng Huo, Hemang Raj Singh, David Quinn, K. Jimmy Hsia, Philip R. LeDuc
Summary: Cell alignment is crucial for various biological functions and tissue engineering applications. This study explores the roles of surface topographies and cell-cell interactions in inducing cell alignment. The results indicate that cell alignment depends on both surface topologies and cell confluency, suggesting the interplay between cell-cell and cell-matrix interactions. Understanding these mechanisms is important for scaffold design, tissue repair, and the development of biohybrid robotic systems.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Gwantaek Kim, Donghyuk Kim, Sukkyung Kang, Jaisuk Yoo, Hyunjung Kim
Summary: The study measured the thermal conductivity of transparent materials - quartz and sapphire glass - using a photothermal deflection method, with relative errors of 5% and 1.5% respectively.
APPLIED SCIENCES-BASEL
(2021)
Review
Biochemical Research Methods
Kerryn Matthews, Erik S. Lamoureux, Marie-Eve Myrand-Lapierre, Simon P. Duffy, Hongshen Ma
Summary: The deformability of red blood cells (RBCs) is crucial for delivering oxygen throughout the body and can serve as a biomarker for disease status and treatment efficacy. Recent advancements in microfluidic technology have greatly improved the sensitivity, throughput, consistency, and ease of use in measuring RBC deformability. These tools have the potential to be used in various applications, such as studying pathologies caused by malaria and hemoglobinopathies, as well as monitoring changes in RBC deformability during blood storage prior to transfusion.
Article
Chemistry, Analytical
Hailong Yu, Qunfei Tai, Chenjie Yang, Lingxiao Weng, Mingxia Gao, Xiangmin Zhang
Summary: This study developed a single-cell picoliter liquid operating technology for counting target proteins in a single cell. The technology demonstrated good linearity and repeatability for ultra-low-copy number proteins, and successfully analyzed the expression level of human epidermal growth factor receptor 2 in single cells from two cell lines.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
V Negi, R. C. Picu
Summary: The study explores the role of fiber entanglements in random athermal fiber networks, showing that entanglements confine fibers, reduce auxetic effect and maintain contact density. The proposed measure of entanglements allows for defining their relationship with fiber volume fraction. Additionally, the effect of inter-fiber friction on the mechanics of entangled networks is evaluated.
Article
Chemistry, Analytical
Chao Wang, Siyuan Xing, Miao Xu, Haodong Shi, Xingkai Wu, Qiang Fu, Huilin Jiang
Summary: This study investigates the design of SR optical system and the influence of optical alignment errors on SR imaging. The SR optical system based on DMD for long-wave infrared wavelength is successfully designed, and thermal analysis of the system is conducted. By introducing various alignment errors and analyzing the reconstructed image quality using the inverse sensitivity method, the tolerance limits of the system during assembly are obtained. The effectiveness of obtaining the alignment tolerance limit of the compression coding SR imaging optical system is verified through a desktop demonstration experiment.
Review
Chemistry, Multidisciplinary
Yiwei Li, Ian Y. Wong, Ming Guo
Summary: This review introduces the fundamental concepts of biomechanics of living cells and recent developments in measuring cell mechanics and controlling cell behavior. It discusses the bidirectional interaction between cells and their microenvironment and its potential relevance for translational medicine. Case studies and emerging technologies are presented to highlight the importance of mechanical measurements in disease diagnosis and therapies.
Article
Biology
Ziqi Zhang, Kelvin C. M. Lee, Dickson M. D. Siu, Michelle C. K. Lo, Queenie T. K. Lai, Edmund Y. Lam, Kevin K. Tsia
Summary: This article introduces an image-based approach that can quantify multiple biophysical fractal-related properties of single cells at subcellular resolution. This technique has high-throughput single-cell imaging performance and offers sufficient statistical power for cell classification, drug response assays, and cell-cycle progression tracking.
COMMUNICATIONS BIOLOGY
(2023)
Article
Hematology
M. Lehmann, K. Ashworth, M. Manco-Johnson, J. Di Paola, K. B. Neeves, C. J. Ng
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2018)
Article
Environmental Sciences
Kenton Rod, Wooyong Um, Jaehun Chun, Ning Wu, Xialong Yin, Guohui Wang, Keith Neeves
JOURNAL OF CONTAMINANT HYDROLOGY
(2018)
Article
Hematology
B. R. Branchford, T. J. Stalker, L. Law, G. Acevedo, S. Sather, C. Brzezinski, K. M. Wilson, K. Minson, A. B. Lee-Sherick, P. Davizon-Castillo, C. Ng, W. Zhang, K. B. Neeves, S. R. Lentz, X. Wang, S. V. Frye, H. Shelton Earp, D. DeRyckere, L. F. Brass, D. K. Graham, J. A. Di Paola
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2018)
Article
Multidisciplinary Sciences
Kathryn G. Link, Michael T. Stobb, Jorge Di Paola, Keith B. Neeves, Aaron L. Fogelson, Suzanne S. Sindi, Karin Leiderman
Article
Robotics
Tao Yang, Andrew Tomaka, Tonguc O. Tasci, Keith B. Neeves, Ning Wu, David W. M. Marr
Editorial Material
Hematology
Hunter B. Moore, Keith B. Neeves
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2019)
Article
Chemistry, Analytical
Matthew G. Sorrells, Keith B. Neeves
Article
Instruments & Instrumentation
E. J. Roth, C. J. Zimmermann, D. Disharoon, T. O. Tasci, D. W. M. Marr, K. B. Neeves
REVIEW OF SCIENTIFIC INSTRUMENTS
(2020)
Article
Hematology
Robert C. Gosselin, Annette Bowyer, Emmanuel J. Favaloro, Jill M. Johnsen, Giuseppe Lippi, Richard A. Marlar, Keith Neeves, Marian A. Rollins-Raval
Summary: Recent shortage of 3.2% sodium citrate blood collection tubes has led stakeholders to seek suitable alternatives, with laboratories particularly concerned about variability between manufacturers and the challenges associated with transitioning to new blood collection methods. This provisional guidance document provides recommendations for validating alternative sources of sodium citrate blood collection tubes before clinical implementation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Dante Disharoon, Brian G. Trewyn, Paco S. Herson, David W. M. Marr, Keith B. Neeves
Summary: This study developed a platform for co-delivering tPA and plasminogen using microwheels to accelerate clot lysis, surpassing the biochemical speed limit through corkscrew motion. The results demonstrated significant efficacy in lysing plasma clots with this approach.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biotechnology & Applied Microbiology
Cara L. Sake, Alexander J. Metcalf, Michelle Meagher, Jorge Di Paola, Keith B. Neeves, Nanette R. Boyle
Summary: This study used isotopically nonstationary C-13 metabolic flux analysis to measure carbon fluxes in resting and activated platelets. The results showed significant differences in metabolic fluxes between activated and resting platelets, providing important insights into the mechanisms of platelet activation.
METABOLIC ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Matthew J. Osmond, Elizabeth Korthals, Coy J. Zimmermann, Eric J. Roth, David W. M. Marr, Keith B. Neeves
Summary: Inflammatory bowel disease (IBD) is caused by the over-expression of tumor necrosis factor-alpha (TNF) in the intestinal mucosa. Oral delivery of anti-TNF is hindered by degradation and poor bioavailability. To overcome these issues, researchers have developed magnetically powered hydrogel particles that can roll along mucosal surfaces, release anti-TNF, and repair damaged cell junctions. This method has the potential to be used for the treatment of IBD.
Article
Chemistry, Multidisciplinary
Aaron K. Ishiki, Keith B. Neeves, David W. M. Marr
Summary: High-molecular-weight polymers that approach the size scale of the gap between the microbots and surfaces can be excluded, impacting microbot transport. This exclusion leads to a reversal in the microbot translation direction.
Article
Biochemical Research Methods
Jess Berry, Francois J. Peaudecerf, Nicole A. Masters, Keith B. Neeves, Raymond E. Goldstein, Matthew T. Harper
Summary: Cardiovascular disease is a leading cause of death worldwide, with myocardial infarction being triggered by blood clots. Developing new anti-platelet drugs is an active area of research, with in vitro models using human blood showing potential in improving relevance. The study developed a microfluidic device for generating occlusive thrombi and demonstrated its ability to monitor the effect of anti-thrombotic drugs in an unbiased and objective manner.
Review
Hematology
Jessica Lin, Matthew G. Sorrells, Wilbur A. Lam, Keith B. Neeves
Summary: This illustrated review delves into the physical forces regulating hemostasis and thrombosis, spanning from vessel to cellular to molecular scales. Blood flow exerts forces on vessel walls and blood cells, impacting the kinetics, structure, and stability of thrombi. External forces and environmental stiffness play crucial roles in platelet function and clot formation.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2021)
