Article
Chemistry, Analytical
Mengmeng Zhang, Xiaoyang Wang, Yajun Zhang, Yiqiang Fan
Summary: This study proposes an integrated microfluidic approach for the quantification of microplastics from river water sediment and biosamples. The proposed two-layer PMMA-based microfluidic device is capable of conducting the sample digestion, filtration, and counting processes inside the microfluidic chip with preprogrammed sequence. The results from the analysis of samples from river water sediment and fish gastrointestinal tract demonstrate that the proposed microfluidic device can perform the quantification of microplastics from river water and biosamples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Natasha Ramnauth, Elise Neubarth, Amy Makler-Disatham, Mazhar Sher, Steven Soini, Vivian Merk, Waseem Asghar
Summary: This study presents a double filtration microfluidic device that can rapidly isolate exosomes via size-exclusion principles in point-of-care settings. The device efficiently isolates exosomes from plasma samples within 50 minutes and exhibits earlier miRNA detection compared to a polyethylene glycol-based method. Comparative analysis of exosomes collected from different pore sizes of membrane filters shows increased sample purity. The developed microfluidic device is cost-efficient and time-efficient, making it ideal for cancer and disease diagnostics and therapeutics in low-resourced and point-of-care settings.
Article
Chemistry, Analytical
Wei Wang, Haleigh M. Grace, Paul A. Flowers
Summary: This study describes a simple microfluidic device that allows spectroelectrochemical measurements on submicroliter samples. By utilizing embedded electrode chips and a solution-cast ionomeric film, measurements on smaller sample volumes can be achieved.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Lael Wentland, Rachel Polaski, Elain Fu
Summary: The study evaluated the dry storage stability of three types of biochemical reagents, demonstrating that they retained activity after being stored dry for approximately one month. Drying the reagents under nitrogen was found to suppress degradation, and devices with dried reagents stored for 15 days showed comparable functionality to devices with freshly-dried reagents.
ANALYTICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Yang Xu, Fangjie Qi, Huachao Mao, Songwei Li, Yizhen Zhu, Jingwen Gong, Lu Wang, Noah Malmstadt, Yong Chen
Summary: This paper presents a general, high-resolution, and low-cost 3D printing process to solve the critical challenge in microfluidic device fabrication. The method achieves high accuracy and efficiency, and is compatible with commonly used light sources and photocurable resins. The verification through multifunctional devices demonstrates the reliability and feasibility of this approach.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Chutikarn Khamkhajorn, Somkid Pencharee, Jaroon Jakmunee, Napaporn Youngvises
Summary: A universal clamp sample holder was designed and fabricated for smartphone-based colorimetric determination of sulfites in wine using a microfluidic cotton swab-based analytical device. The method involves converting sulfite to volatile SO2, which reacts with Fe(III) to form a red complex. The mu CSAD is cost-effective and eco-friendly, with a mobile software application for calibration and sulfite concentration evaluation.
MICROCHEMICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Ashwin Kumar Vutha, Ryan Patenaude, Alexis Cole, Rajesh Kumar, John N. Kheir, Brian D. Polizzotti
Summary: The paper introduces a method of delivering oxygen to patients through intravenous injection, which can replace or supplement the function of injured lungs and provide effective oxygen supply for critically ill patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Environmental
Seong-Geun Jeong, Dong-Ho Kim, Jingyeong Kim, Ji-Hyun Kim, Sanggeun Song, Chang-Soo Lee
Summary: This study presents a method to achieve automatic multistep digital analysis in a single-sheet 3D paper-based microfluidic analytical device (3D-mu PAD) by controlling the penetration of different wax inks into paper. The methodology allows for sequential fluid delivery and multistep biochemical reactions, which can be integrated with digital assays without the need for external instruments. The potential commercial application of this approach is significant.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Analytical
Somayyeh Bakhtiaridoost, Hamidreza Habibiyan, Hassan Ghafoorifard
Summary: This study presents a fast, hemolysis-free, highly efficient blood plasma separation microfluidic device that combines gravitational sedimentation with dielectrophoresis force. Using finite element analysis, the effect of geometrical parameters on the separation process is investigated to achieve optimal design specifications. The device achieves a purity of 99.98% and a relative increase in flow rate compared to previous studies by utilizing a porous Melt-Blown Polypropylene layer and optimizing the dielectrophoresis force.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Zihan Yang, Zhihang Zhou, Tongxu Si, Zhengdong Zhou, Li Zhou, Y. Rebecca Chin, Liang Zhang, Xinyuan Guan, Mengsu Yang
Summary: Cancer metastasis is a major cause of cancer-related death, and it is characterized by excessive extracellular matrix deposition and increased stiffness in solid tumors. The confined migration of tumor cells in these conditions plays a crucial role in metastasis, but inhibitors specifically targeting this type of migration are rare. In this study, a microfluidic chip was designed to screen for drugs that can effectively inhibit confined migration of cancer cells. By applying this chip, three novel inhibitors of confined migration, MA-5, SB-705498, and diphenyleneiodonium chloride, were discovered in multiple cancer types. The mechanisms of action of these inhibitors were found to be targeting mitochondria, actin polymerization, and cell viability, respectively. Overall, a high-throughput microfluidic platform for screening drugs targeting confined migration was established, and three effective inhibitors were identified.
Review
Biochemical Research Methods
Katherine S. Elvira, Fabrice Gielen, Scott S. H. Tsai, Adrian M. Nightingale
Summary: Droplet-based systems have become a compelling tool in microbiological and (bio)chemical science. This article summarizes the materials and fabrication techniques required to make microfluidic devices that deliver controlled uniform droplet flow, and discusses the future development and challenges in this field.
Article
Chemistry, Multidisciplinary
Li Huang, Enben Su, Yuan Liu, Nongyue He, Yan Deng, Lian Jin, Zhu Chen, Song Li
Summary: The developed microfluidic chip in this study aims to improve the accuracy of the chromatography system, with significant improvements in detection sensitivity and determination correlation. By enhancing the mixing between the sample and microspheres, the coefficient of variation of the traditional strip can be effectively increased.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Franciele Flores Vit, Rony Nunes, Yu Tzu Wu, Marco Cesar Prado Soares, Naiara Godoi, Eric Fujiwara, Hernandes F. Carvalho, Lucimara Gaziola de la Torre
Summary: This study introduces a microfluidic technology for drug screening that enables the assessment of cellular behavior in dynamic conditions and the recovery of cells for subsequent processing and imaging.
ANALYTICA CHIMICA ACTA
(2021)
Article
Materials Science, Multidisciplinary
Jose M. de Hoyos-Vega, Hye Jin Hong, Kevin Loutherback, Gulnaz Stybayeva, Alexander Revzin
Summary: Liver cultures have evolved from hepatocyte monocultures to more complex co-cultures and precision-cut liver slices. This study presents a microfluidic device that allows for longer-term maintenance of functional organotypic liver cultures, displaying promising potential for disease modeling and individualized therapies.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Analytical
Chunyang Wei, Chengzhuang Yu, Shanshan Li, Jiyu Meng, Tiejun Li, Jingmeng Cheng, Junwei Li
Summary: This study proposes a droplet-based multivolume microfluidic device for easy-to-operate and integrated droplet digital PCR (ddPCR), achieving DNA molecule quantification with a wide dynamic range, easy operation, and miniaturization.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Biochemical Research Methods
Weiyuan Lyu, Mengchao Yu, Haijun Qu, Ziqing Yu, Wenbin Du, Feng Shen
Article
Nanoscience & Nanotechnology
Elysia A. Masters, Alec T. Salminen, Stefano Begolo, Emma N. Luke, Sydney C. Barrett, Clyde T. Overby, Ann Lindley Gill, Karen L. de Mesy Bentley, Hani A. Awad, Steven R. Gill, Edward M. Schwarz, James L. McGrath
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2019)
Article
Chemistry, Multidisciplinary
Haijun Qu, Mengchao Yu, Wenbin Du, Lei Xu, Weiyuan Lyu, Feng Shen
Article
Biophysics
Ziqing Yu, Weiyuan Lyu, Mengchao Yu, Qian Wang, Haijun Qu, Rustem F. Ismagilov, Xu Han, Dongmei Lai, Feng Shen
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Analytical
Yan Yu, Ziqing Yu, Xufeng Pan, Lei Xu, Rui Guo, Xiaohua Qian, Feng Shen
Summary: Digital PCR is a method that can accurately and precisely quantify target nucleic acids. The use of digital melting curve analysis (digital MCA) allows for the multiplex quantification of multiple target molecules in a single digital PCR. This method uses a self-partitioning SlipChip microfluidic system, which can generate microdroplets with trackable physical positions. The unique melting temperature (T-m) of amplification products can be used as a fingerprint for further classification.
Article
Chemistry, Multidisciplinary
Xu Liu, Xiang Li, Nannan Wu, Yang Luo, Jiajie Zhang, Ziqing Yu, Feng Shen
Summary: The study presents a novel microfluidic device that enables instrument-free gradient droplet formation and parallel manipulation for screening tests. The device demonstrates good performance in antimicrobial susceptibility testing and shows consistent results with clinical culture-based tests.
Article
Chemistry, Multidisciplinary
Qixin Hu, Fariha Kanwal, Weiyuan Lyu, Jiajie Zhang, Xu Liu, Kai Qin, Feng Shen
Summary: Pancreatic cancer is a lethal disease with low survival rates. Researchers have developed a droplet-array SlipChip for the detection of KRAS mutations in pancreatic cancer using digital PCR. This high-density microwell array allows for accurate quantification of mutant genes and wild-type KRAS gene. The results obtained from this chip align well with those from next-generation sequencing, making it a promising tool for point-of-care applications.
Article
Chemistry, Analytical
Xiang Li, Qixin Hu, Xu Liu, Jianfang Liu, Nannan Wu, Feng Shen
Summary: The rise of antimicrobial resistance is a global health concern, and there is a need for new antimicrobial drugs and alternative therapies. Phage therapy has shown promising results as an alternative treatment for antimicrobial resistance. Researchers have developed a digital biosensing method on a microfluidic device for rapid quantification of infectious bacteriophages. This method provides a promising tool for phage quantification in clinical practice and can also be used for ultrasensitive and high-specificity bacteria detection.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yan Yu, Sheng Wang, Yang Luo, Chang Gu, Xin Shi, Feng Shen
Summary: Methylation is an essential epigenetic modification involved in regulating gene expression and maintaining genome stability. Current methods of methylation analysis face limitations of high cost, time-consuming workflows, and difficulty in accurate heterogeneity analysis and precise quantification. A droplet array SlipChip-based digital melting curve analysis (MCA) method was developed for accurate quantification of both methylation level and density. This method has been demonstrated to distinguish healthy individuals from patients with atrial fibrillation and holds potential for broader application in various research and clinical settings requiring methylation analysis.
Article
Biochemical Research Methods
Xiang Li, Xu Liu, Ziqing Yu, Yang Luo, Qixin Hu, Zhenye Xu, Jia Dai, Nannan Wu, Feng Shen
Summary: Bacterial antimicrobial resistance is a global threat, and a rapid high-throughput phenotypic antimicrobial susceptibility testing (AST) method is needed. The cs-SlipChip, containing 192 nanoliter-sized compartments, allows for high-throughput AST by monitoring bacterial growth and analyzing changes in bright-field imaging. The chip can measure the minimum inhibitory concentration (MIC) of Escherichia coli against four antibiotics within 3 hours and has been validated against a VITEK 2 automated system. This technology provides a practical and rapid method for AST and screening antibiotic combinations for treating multiple antibiotic-resistant bacteria.
Article
Biochemical Research Methods
Ziqing Yu, Lei Xu, Weiyuan Lyu, Feng Shen
Summary: Digital biological analysis compartmentalizes targets of interest and performs detection and investigation. This study presents a parallel multistep digital analysis SlipChip, which enables the manipulation of a large number of droplets for digital biological analysis. The SlipChip utilizes a self-partitioning droplet formation mechanism and can perform digital loop-mediated isothermal amplification and digital CRISPR detection in parallel.
Article
Materials Science, Multidisciplinary
Jin Li, Chang He, Haijun Qu, Feng Shen, Jian Ye
Summary: The study introduces the design of O-GERTs for creating PUF security labels, achieving a large encoding capacity and high information density. Digitized from signals of embedded alkyne Raman reporters, the PUF labels exhibit robustness against interference and demonstrate high reliability.
JOURNAL OF MATERIALS CHEMISTRY C
(2022)
Article
Biochemical Research Methods
Weiyuan Lyu, Jiajie Zhang, Yan Yu, Lei Xu, Feng Shen
Summary: The da-SlipChip is a novel microfluidic device that can generate droplets of various sizes at high density in a single slipping step, without requiring precise alignment of microfeatures, thus significantly increasing design flexibility and partition density.
Review
Multidisciplinary Sciences
Chang Gu, Xin Shi, Chenyang Dai, Feng Shen, Gaetano Rocco, Jiafei Chen, Zhengyu Huang, Chunji Chen, Chuan He, Tao Huang, Chang Chen
Review
Materials Science, Biomaterials
Daniel Garcia Alonso, Mengchao Yu, Haijun Qu, Liang Ma, Feng Shen
ADVANCED BIOSYSTEMS
(2019)