Journal
LAB ON A CHIP
Volume 12, Issue 20, Pages 4055-4058Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc40584a
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Funding
- NSF
- Burroughs Wellcome Fund Career Award at the Scientific Interface
- James H. Ferry Fund for Innovation
- F R Meyer III [1942]
- G S Meyer UROP Fund
- Joseph R. Mares endowed chair
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Although polymerization-based amplification (PBA) has demonstrated promise as an inexpensive technique for use in molecular diagnostics, oxygen inhibition of radical photopolymerization has hindered its implementation in point-of-care devices. The addition of 0.3-0.7 mu M eosin to an aqueous acrylate monomer solution containing a tertiary amine allows an interfacial polymerization reaction to proceed in air only near regions of a test surface where additional eosin initiators coupled to proteins have been localized as a function of molecular recognition events. The dose of light required for the reaction is inversely related to eosin concentration. This system achieves sensitivities comparable to those reported for inert gas-purged systems and requires significantly shorter reaction times. We provide several comparisons of this system with other implementations of polymerization-based amplification.
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