Article
Biochemical Research Methods
Huichao Chai, Yongxiang Feng, Fei Liang, Wenhui Wang
Summary: In this paper, a novel microfluidic device with high-efficiency cell trapping and deterministic release is presented. The device includes capillary valves that can open in a predefined pattern to sequentially trap incoming cells into separate sites. Experiments using MCF-7 cells demonstrated the capability of the device for deterministic single cell trapping and release, showing promise as a useful platform for single cell analysis.
Article
Multidisciplinary Sciences
Yang Xu, Fangjie Qi, Huachao Mao, Songwei Li, Yizhen Zhu, Jingwen Gong, Lu Wang, Noah Malmstadt, Yong Chen
Summary: This paper presents a general, high-resolution, and low-cost 3D printing process to solve the critical challenge in microfluidic device fabrication. The method achieves high accuracy and efficiency, and is compatible with commonly used light sources and photocurable resins. The verification through multifunctional devices demonstrates the reliability and feasibility of this approach.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Lei Zhu, Jianan Wang, Jianwei Liu, Zhicheng Xu, Muhammad Salman Nasir, Xin Chen, Ze Wang, Shiyi Sun, Qianyue Ma, Jinbo Liu, Jiangtao Feng, Jidong Liang, Wei Yan
Summary: The current gas sensors suffer from insufficient sensitivity and selectivity, limiting their application in real-time monitoring of low concentration gas. This study proposes a versatile in situ enrichment amplification strategy that effectively enhances the detection sensitivity and selectivity of gas sensors for hazardous trace gases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Biomedical
Ilana Berger Fridman, James Kostas, Michal Gregus, Somak Ray, Matthew R. Sullivan, Alexander R. Ivanov, Smadar Cohen, Tania Konry
Summary: Cancer progression is driven by changes in tumor cells and surrounding stroma. The study demonstrates a high throughput microfluidic system for generating dynamic tumor-stroma scaffolds that mimic in vivo TME, with Alg/Alg-S promoting in vivo-like responses and potential therapeutic targets for breast cancer treatment. Formation of large tumor-derived vesicles in Alg/Alg-S scaffolds suggests a mechanism for tumor survival.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Multidisciplinary
Hongyu Li, Rang Chu, Jinyuan Cao, Fei Zhou, Kuikui Guo, Qingmao Zhang, Hongcheng Wang, Ye Liu
Summary: The combination of surface-enhanced Raman scattering (SERS) and microfluidic technology is utilized to improve the reproducibility and reliability of SERS detection. Researchers develop a side-polished multimode fiber (SPMF) SERS probe integrated into a microfluidic channel to form a glass-based microfluidic-SERS chip. The optimized chip demonstrates low detection limits and good spectral reproducibility, providing a highly sensitive and reproducible SERS detection method with potential applications in environmental science, food safety, and biomedicine.
Article
Chemistry, Analytical
Shan He, Huiting Lian, Xuegong Cao, Bin Liu, Xiaofeng Wei
Summary: Non-invasive body fluid analysis has made significant progress in health monitoring and disease diagnostics. In this study, a wearable microfluidic device with a cascaded enzymatic reaction-mediated multicolor pixelated quantitative system was developed for non-invasive and sensitive detection of sweat glucose. This is the first application of non-invasive portable detection using multicolor pixelated quantification, and also the first smartphone-assistant multicolor pixelated analysis for Localized Surface Plasmon Resonance (LSPR) of gold nanorods (Au NRs).
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Neda Azizipour, Rahi Avazpour, Michael H. Weber, Mohamad Sawan, Abdellah Ajji, Derek H. Rosenzweig
Summary: The use of a surface-optimized PDMS microfluidic biochip allows for the reproducible generation of uniform multicellular spheroids, which can more accurately predict cellular responses to drugs in vitro. This platform has important clinical implications for developing new therapeutic agents and optimizing existing treatment strategies in personalized medicine.
Article
Multidisciplinary Sciences
Ratul Paul, Yuwen Zhao, Declan Coster, Xiaochen Qin, Khayrul Islam, Yue Wu, Yaling Liu
Summary: Microfluidic devices have limitations in terms of low resolution, inferior feature fidelity, and poor repeatability. This study proposes a strategic approach called image-guided in-situ maskless lithography (IGIs-ML) to address these challenges. By using dynamic image analysis and flow control, IGIs-ML provides superior repeatability and fidelity for densely packed features across a large area. This approach enables the fabrication of a wide variety of microfluidic devices and resolves critical limitations in rapid prototyping.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Wei-Ting Tseng, Hsin-Yi Tseng, Yin-Yu Chou, Yin-Chen Wang, Tz-Ning Tseng, Li-Ing Ho, Sheng-Wei Pan, Mei-Lin Ho
Summary: The study developed a urinary tract infection diagnostic method based on microfluidic resistance sensors for early detection and quantitative analysis of LE. Using LE-Ag-mu PADs and LE-Ag-ZnO-mu PADs enables sensitive detection of LE, with improved detection capabilities at lower concentrations compared to traditional ELISA methods.
DALTON TRANSACTIONS
(2021)
Article
Nanoscience & Nanotechnology
Lucio Litti, Stefano Trivini, Davide Ferraro, Javier Reguera
Summary: Surface-enhanced Raman scattering (SERS) is an ideal technique for environmental and biomedical sensor devices due to its highly informative vibrational features and ultrasensitive nature. The use of Janus magnetic/plasmonic Fe3O4/Au nanostars (JMNSs) as SERS colloidal substrates allows for quantitative determination of analytes with the additional amplification of SERS signals using an external magnetic field, showing promising applications in various fields.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemical Research Methods
Hamed Tavakoli, Elisabeth Hirth, Man Luo, Sanjay Sharma Timilsina, Maowei Dou, Delfina C. Dominguez, XiuJun Li
Summary: In this research, a microfluidic fully paper-based analytical device (mu FPAD) integrated with loop-mediated isothermal amplification (LAMP) and ssDNA-functionalized graphene oxide (GO) nano-biosensors was developed for the first time for simple, rapid, low-cost, and quantitative detection of Neisseria meningitidis (N. meningitidis). The presented mu FPAD offers versatile functions and is capable of a simple, highly sensitive, and specific diagnosis of N. meningitidis. Furthermore, this microfluidic approach has great potential in the rapid detection of a wide variety of different other pathogens in low-resource settings.
Article
Polymer Science
Elham Badali, Mahshid Hosseini, Negar Varaa, Narges Mahmoodi, Arash Goodarzi, Vajihe Taghdiri Nooshabadi, Sajad Hassanzadeh, Zohreh Arabpour, Mehdi Khanmohammadi
Summary: This study successfully developed cell-laden silk fibroin-phenol (SF-Ph) microparticles using a co-flow microfluidic system. The microparticles showed high cell survival rate and facilitated cellular proliferation, demonstrating their potential in tissue engineering and regenerative medicine applications.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Biochemical Research Methods
Qihong Ning, Wei Zheng, Hao Xu, Armando Zhu, Tangan Li, Yuemeng Cheng, Shaoqing Feng, Li Wang, Daxiang Cui, Kan Wang
Summary: This paper presents the fabrication of multi-layer mu PADs for colorimetric detection of CRP using the imprinting method. The detection performance of mu PADs is improved through simulating different lighting conditions and shooting angles, and a machine learning algorithm is used for analysis. The results show that the YOLO model trained in this study can accurately identify all reaction areas, and the residual network algorithm achieves the highest accuracy in the classification task.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
K. Arias-Alpizar, A. Sanchez-Cano, J. Prat-Trunas, E. de la Serna Serna, O. Alonso, E. Sulleiro, A. Sanchez-Montalva, A. Dieguez, E. Baldrich
Summary: A point-of-care device for sensitive and selective detection of malaria infection biomarker using a single-step magneto-immunoassay has been reported, providing quantitative results in less than 20 minutes with high sensitivity. This device shows great potential to simplify testing procedures and improve detection accuracy.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Yagmur Demircan Yalcin, Taylan Berkin Toral, Sertan Sukas, Ender Yildirim, Ozge Zorlu, Ufuk Gunduz, Haluk Kulah
Summary: The development of a lab-on-a-chip system enabled the investigation of drug resistance in leukemia cells using coupled dielectrophoretic detection (DEP-D) and impedimetric counting (IM-C) techniques. Conductivity matching and frequency values allowed for successful discrimination between drug resistant and wild type cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Bo Tian, Gabriel Antonio S. Minero, Jeppe Fock, Martin Dufva, Mikkel Fougt Hansen
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Joen H. Jensen, Selgin D. Cakal, Jingwen Li, Christian J. Pless, Carmen Radeke, Morten Leth Jepsen, Thomas E. Jensen, Martin Dufva, Johan U. Lind
SCIENTIFIC REPORTS
(2020)
Article
Biophysics
Bo Tian, Fei Gao, Jeppe Fock, Martin Dufva, Mikkel Fougt Hansen
BIOSENSORS & BIOELECTRONICS
(2020)
Letter
Immunology
John Rizk, Rasmus Agerholm, Alexander Jonsson, Asli Aybike Dogan, Martin Dufva, Vasileios Bekiaris
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Arianna Toppi, Louise L. Busk, Hongxia Hu, Asli A. Dogan, Alexander Jonsson, Rafael J. Taboryski, Martin Dufva
Summary: FluorAcryl 3298 (FA) was utilized for fabricating microstructured surfaces with hydrophilic-in-hydrophobic characteristics, achieving high-resolution microwell arrays and demonstrating its potential applications in digital immunoassays and other fields.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Arianna Toppi, Martin Dufva
Summary: A new method for fabricating microdroplet array (MDA) substrates independently from cleanroom processes was developed. A low-cost in-house built system for photopatterning a negative resist was assembled to produce stable and high-quality MDAs. The feasibility for carrying out digital assays was demonstrated.
Article
Multidisciplinary Sciences
Asli Aybike Dogan, Martin Dufva
Summary: There is a high demand for complex cell cultures in various fields, with Transwell inserts being popular due to their commercial availability, ease of use, and versatility. Customizing inserts can offer potential benefits in terms of layers, height, and membrane composition, allowing for tailored experiments and improved culture formation.
SCIENTIFIC REPORTS
(2022)
Correction
Multidisciplinary Sciences
Asli Aybike Dogan, Martin Dufva
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Stephen R. Doyle, Martin Jensen Soe, Peter Nejsum, Martha Betson, Philip J. Cooper, Lifei Peng, Xing-Quan Zhu, Ana Sanchez, Gabriela Matamoros, Gustavo Adolfo Fontecha Sandoval, Cristina Cutillas, Louis-Albert Tchuem Tchuente, Zeleke Mekonnen, Shaali M. Ame, Harriet Namwanje, Bruno Levecke, Matthew Berriman, Brian Lund Fredensborg, Christian Moliin Outzen Kapel
Summary: This study provides the first population genomics analysis of the whipworm Trichuris trichiura, a soil-transmitted helminth that causes trichuriasis in humans. By sequencing the whole genomes of modern and ancient samples, the authors characterize the genetic diversity and evolution of this pathogen. The findings suggest an African origin of T. trichiura with subsequent human migration, and reveal genetic differentiation among geographically distinct populations. This study sheds light on zoonotic reservoirs of human-infective T. trichiura and will aid in genomic epidemiology efforts.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Maria Niora, Mathilde Hauge Lerche, Martin Dufva, Kirstine Berg-Sorensen
Summary: Fluorescent nanodiamonds (FNDs) with negative nitrogen-vacancy (NV-) defect centers are ideal probes for biosensing applications and can potentially serve as biomarkers for cell differentiation. The uptake of FNDs by THP-1 monocytes and monocyte-derived M0-macrophages is studied, revealing differing FND-cell interactions and time-dependent uptake. FND uptake allows for distinction between cell subtypes based on phagocytic capacity, and FNDs demonstrate effective cell labeling and have potential for sensing biological processes involving cell differentiation.
Article
Biotechnology & Applied Microbiology
Asli Aybike Dogan, Martin Dufva
Summary: This study introduces a customized hanging insert for cultivating Caco-2 cells on hydrogel membranes under flow conditions. The device consists of an apical channel-like chamber for cell culture with shear force and a basolateral chamber that is mixed when rocked. The experiment investigates the impact of seeding density and flow on the growth of the cells. The results show that low seeding density and flow promote the formation of three-dimensional structures and improve barrier function.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
Martin Dufva
Summary: Out of 2828 screened articles, only 146 contained controls and quantified data for flow cell culture. Analysis of these articles showed that many biomarkers in all cell types were unaffected by flow, while only specific biomarkers responded strongly to flow.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Electrical & Electronic
Alexander Jonsson, Arianna Toppi, Martin Dufva
Article
Materials Science, Biomaterials
Morten Leth Jepsen, Andreas Willumsen, Chiara Mazzoni, Anja Boisen, Line Hagner Nielsen, Martin Dufva
ADVANCED BIOSYSTEMS
(2020)
Article
Engineering, Biomedical
Salime Bazban-Shotorbani, Felicity Gavins, Krishna Kant, Martin Dufva, Nazila Kamaly
Summary: A dysfunctional endothelium is a hallmark of many diseases, including atherosclerosis. Researchers found that size plays a dominant role in nanoparticle targeting, with nanoparticles in the range of 30-60nm showing increased targeting to dysfunctional endothelium using a biomicrofluidic model. Additionally, treating dysfunctional endothelium with Annexin A1 resulted in restoration of adherens junctions and normalization of barrier integrity.
ADVANCED NANOBIOMED RESEARCH
(2022)