Article
Chemistry, Analytical
Szabolcs Fekete, Balazs Bobaly, Jennifer M. Nguyen, Alain Beck, Jean-Luc Veuthey, Kevin Wyndham, Matthew A. Lauber, Davy Guillarme
Summary: In liquid chromatography, using short columns may offer similar separation quality to using long columns and enable faster analysis. Research on the elution behavior of therapeutic monoclonal antibodies and their fragments using modern instrumentation and column formats revealed that narrow-bore columns can separate species quickly and efficiently.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Audrius Maruska, Tomas Drevinskas, Mantas Stankevicius, Kristina Bimbiraite-Surviliene, Vilma Kaskoniene, Linas Jonusauskas, Roaldas Gadonas, Staffan Nilsson, Olga Kornysova
Summary: This work describes the design and characterization of a multi-cell capacitively coupled contactless conductivity detection system, capable of simultaneously acquiring data from multiple detector cells and easily upgradable to 64 detection cells. The system demonstrated sensitivity to low concentrations of various solutions in water and the ability to distinguish different mixtures of organic solvents. It is expected to be used in conjunction with multi-channel separation devices for monitoring simultaneous processes.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Yaqin Nan, Pingyi Zheng, Mengqi Cheng, Ran Zhao, Haijiao Jia, Qinggang Liang, Youxin Li, James J. Bao
Summary: A water-soluble negative sulfonic propyl ether β-CD polymer (SPE-β-CDP) was successfully polymerized as a chiral selector in capillary electrophoresis (CE) and its separation ability was verified. The results showed that the polymer had a lower effect on electrical current of CE, indicating high concentration could be added, and it had good water solubility and easy preparation.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
H. Dennis Tolley, Samuel Avila, Brian D. Iverson, Austin R. Foster, Aaron R. Hawkins, Samuel E. Tolley, Milton L. Lee
Summary: This study introduces a method for simulating molecular transport in capillary gas chromatography, which has been experimentally validated. The model provides reasonable predictions for isothermal, temperature-programmed, and thermal gradient GC separations. The relatively low errors in simulated peak elution time and width demonstrate the model's ability to reflect transport processes and predict meaningful outcomes for GC separations.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Ang Li, Song Xue, Yu Xu, Sihui Ding, Di Wen, Qi Zhang
Summary: The study explores the feasibility of using hydrophobic eutectic solvents (HESs) as pseudo-stationary phases (PSPs) in capillary electrophoresis (CE) for chiral separations. It demonstrates that there is a potential synergistic effect between HESs and traditional chiral selectors, improving enantioseparations of basic drugs.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Zoltan Juvancz, Rita Bodane-Kendrovics, Zita Laczko, Robert Ivanyi, Erzsebet Varga
Summary: Pyrethroid insecticides are highly toxic to insects and fish but have low toxicity for warm-blooded living creatures. In order to reduce their environmental impact, the use of enantio-pure insecticide products instead of racemic mixtures is important. This study focused on the chiral analysis and separation of pyrethroic acids, an intermediate of pyrethroids. Various enantiomeric selective chromatographic methods were used, and beta-cyclodextrin was found to be excellent for the chiral separation of these acids. The results of this study provided insights into the correlations between chiral selectivity features of selectors and analyte structures.
Article
Chemistry, Analytical
Nuzhet Nihaar Nasir Ahamed, Carlos A. Mendiola-Escobedo, Olivia D. Ernst, Victor H. Perez-Gonzalez, Blanca H. Lapizco-Encinas
Summary: This study demonstrates that electrokinetic phenomena can be used to develop rapid and reliable methods for microparticle and cell assessments in a cost-effective and label-free manner. By using direct current (DC)-biased low-frequency alternating current (AC) voltages in an insulator-based electrokinetic (iEK) system, microparticles with a small difference in zeta potentials can be effectively separated.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Blanca H. Lapizco-Encinas, Y. Victoria Zhang, Putuma P. Gqamana, Jana Lavicka, Frantisek Foret
Summary: Capillary electrophoresis (CE) is a powerful separation technique that offers high resolution, great sensitivity, and short processing times. When combined with mass spectrometry (MS), CE-MS systems provide accurate characterization of ionized species and significantly enhance the capabilities of MS. This review article introduces the analytical potential of CE-MS systems and discusses recent advances and future developments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Renata Konasova, Maria Butnariu, Veronika Solinova, Vaclav Kasicka, Dusan Koval
Summary: Charged polymer coatings on the inner fused silica capillary wall enable tunable electroosmotic flow velocity for optimizing peak resolution and separation efficiency in capillary electrophoresis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Mari-Luiza Konjaria, Rusudan Kakava, Alessandro Volonterio, Bezhan Chankvetadze, Gerhard K. E. Scriba
Summary: This study evaluated the enantioseparation of 16 chiral compounds using capillary electrophoresis with charged cyclodextrins as chiral selectors. It found that randomly substituted cyclodextrins showed good separation ability for the model analytes. The study also discovered the influence of complexation constants and complex mobilities on the migration order of enantiomers.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Raymond B. Yu, Joselito P. Quirino
Summary: Native cyclodextrins were studied as chiral selectors in open-tubular admicellar electrochromatography using CTAB as dynamic stationary pseudophase. An increase in the CD concentration decreased the magnitude of the EOF, leading to reduced retention factors of the tested analytes as no admicelles or stationary pseudophase were formed under certain conditions.
MICROCHEMICAL JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Sami El Deeb, Camilla Fonseca Silva, Clebio Soares Nascimento Junior, Rasha Sayed Hanafi, Keyller Bastos Borges
Summary: This review article summarizes the literature on chiral capillary electrokinetic chromatography from January 2016 to March 2021, focusing on the separation of drugs and other chiral molecules. It discusses measures to enhance detection sensitivity, chiral recognition techniques, as well as the application of Quality by Design principles and molecular modeling.
Article
Chemistry, Analytical
Yuling Li, Bowen Zhou, Keke Wang, Jing Zhang, Wenjian Sun, Li Zhang, Yinlong Guo
Summary: The mass-tagged chiral selector [d(0)]/[d(5)]-17 beta-EBC was successfully developed for the separation and identification of amino acid enantiomers, achieving good enantiomeric resolution for 19 chiral proteinogenic AAs in a quick analytical run. The linear calibration curve of enantiomeric excess using [d(0)]/[d(5)]-17 beta-EBC showed promising results, demonstrating its potential in peptide drug quality control and discovery of chiral disease biomarkers.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Chemical
Qi Zhang, Siru Ren, Song Xue, Ang Li, Siyao Liu, Xiaodong Sun
Summary: The study evaluated six novel ionic liquids as chiral selectors in capillary electrophoresis, showing better enantioseparation performance compared to traditional chiral selectors.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Tingting Hong, Xing Liu, Yibing Ji, Songwen Tan, Zhiqiang Cai
Summary: Novel chiral capillary electrochromatography (CEC) microsystems were constructed based on Aspergillus sp. CM96. As cell chirality is a newly discovered intrinsic characteristic of the cell, it plays an essential role in life evolution. Aspergillus sp. CM96 spore (CM96s) was chosen to develop chiral capillary columns, and baseline separation of various amino acid (AA) enantiomers was achieved under optimized conditions. The time-dependent chiral interactions between AAs and CM96s were also explored. Immobilization of pectinases generated from Aspergillus sp. CM96 fermentation onto graphene oxide-functionalized capillary silica monoliths facilitated the separation of AA enantiomers. Molecular docking simulations were performed to understand the chiral separation mechanisms of pectinase for AA enantiomers. These results highlight the significant advantage of Aspergillus sp. CM96-based CEC microsystems in chiral separation.
Article
Biotechnology & Applied Microbiology
Xiaopu Wang, Javier Atencia, Roseanne M. Ford
BIOTECHNOLOGY AND BIOENGINEERING
(2015)
Article
Biochemical Research Methods
S. Garcia, R. Sunyer, A. Olivares, J. Noailly, J. Atencia, X. Trepat
Article
Physics, Applied
A. G. Yew, D. Pinero, A. H. Hsieh, J. Atencia
APPLIED PHYSICS LETTERS
(2013)
Article
Cell & Tissue Engineering
Alvin G. Yew, Javier Atencia, Adam H. Hsieh
CELLULAR AND MOLECULAR BIOENGINEERING
(2014)
Article
Biochemical Research Methods
Gregory A. Cooksey, Anne L. Plant, Javier Atencia
Article
Biochemical Research Methods
Javier Atencia, Jayne Morrow, Laurie E. Locascio
Article
Biochemical Research Methods
Javier Atencia, Gregory A. Cooksey, Andreas Jahn, Justin M. Zook, Wyatt N. Vreeland, Laurie E. Locascio
Article
Biochemical Research Methods
Javier Atencia, Gregory A. Cooksey, Laurie E. Locascio
Article
Biochemical Research Methods
Renee R. Hood, Don L. DeVoe, Javier Atencia, Wyatt N. Vreeland, Donna M. Omiatek
Article
Biochemical Research Methods
Gregory A. Cooksey, Javier Atencia
Editorial Material
Biochemical Research Methods
Gregory A. Cooksey, Javier Atencia, Samuel P. Forry
Meeting Abstract
Cell Biology
S. Garcia, R. Sunyer, J. Atencia, X. Trepat
MOLECULAR BIOLOGY OF THE CELL
(2014)
Meeting Abstract
Chemistry, Multidisciplinary
Xiaopu Wang, Javier Atencia, Roseanne M. Ford
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2012)
Meeting Abstract
Chemistry, Multidisciplinary
Laurie E. Locascio, David Ross, Wyatt N. Vreeland, Samuel Forry, Matthew Munson, Jason G. Kralj, Javier Atencia, Darwin R. Reyes, Michael Gaitan
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2008)