Article
Chemistry, Analytical
Yesim Tugce Yaman, Gulcin Bolat, Turkan Busra Saygin, Serdar Abaci
Summary: The study reported the fabrication of a molecularly imprinted overoxidized polypyrrole MIP(oPPy) on graphene oxide (GO) modified single-use pencil graphite electrode (PGE) for the quantitative analysis of the food processing contaminant 3-MCPD. Through optimizing experimental conditions, the research achieved superior recognition performance for 3-MCPD, indicating significant promise for future food safety control applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Jingbo Zhang, Quancheng Chen, Xuemin Gao, Qian Lin, Ziqin Suo, Di Wu, Xijie Wu, Qing Chen
Summary: In this study, a label-free and antibody-free impedimetric biosensor based on molecularly imprinting technology was developed for quantifying exosomes derived from non-small-cell lung cancer (NSCLC) cells. The sensor showed high sensitivity and selectivity with an LOD of 2.03 x 10(3) and an LOQ of 4.10 x 10(4) particles/mL. It demonstrated great accuracy, precision, and retention of performance after multiple cycles of elution and re-adsorption. The sensor has potential clinical translational applications in improving the prognosis and survival of NSCLC patients.
Article
Chemistry, Multidisciplinary
Aleksandr Egunov, Zehua Dou, Dmitriy D. Karnaushenko, Franziska Hebenstreit, Nicole Kretschmann, Katja Akgun, Tjalf Ziemssen, Daniil Karnaushenko, Mariana Medina-Sanchez, Oliver G. Schmidt
Summary: An innovative rolled-up impedimetric microfluidic sensor, named sensor-in-a-tube, is introduced for simultaneous analysis of single human monocytes CD14+ and their extracellular medium during lipopolysaccharide (LPS)-induced activation. The platform utilizes electrical impedance spectroscopy to detect the correspondence between cell activation stages and the electrical properties of the cell surrounding medium without the need for surface functionalization or labeling. The sensor-in-a-tube platform is demonstrated as a sensitive and reliable tool for precise single cell analysis towards immune-deficient diseases diagnosis.
Article
Chemistry, Analytical
Xiaoyan Li, Natashya Falcone, M. Nur Hossain, Heinz-Bernhard Kraatz, Xiaojun Chen, He Huang
Summary: A novel label-free impedimetric electrochemical sensor was proposed for the detection of OTA in food products. The sensor achieved signal amplification by introducing RecJf exonuclease, showing promising potential for application in the food industry.
Article
Chemistry, Analytical
Gulcin Bolat, Oznur Akbal Vural, Yesim Tugce Yaman, Serdar Abaci
Summary: A sensor platform using PNT-GO structures for quantification of diabetic nephropathy and cancer-related microRNA-192 was prepared and characterized. The impedance signal was utilized to evaluate hybridization between miRNA-192 and its DNA probe, showing high sensitivity and a wide detection range. Evaluation in peripheral serum samples demonstrated the potential for future clinical and diverse biomedical applications.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Fateme Ebrahimi, Hossein Salar Amoli, Sayed Ahmad Mozaffari
Summary: In this study, a novel approach for determining lactoferrin was developed using a mixed monolayer of MOP/3-sulfanylpropan-1-ol as a biosensing platform. Various techniques were employed to track the platform fabrication process and evaluate its efficiency for lactoferrin determination. The results showed that the developed platform exhibited good selectivity and sensitivity towards lactoferrin detection, and was successfully applied for determining lactoferrin in colostrum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Lian C. T. Shoute, Gaser N. Abdelrasoul, Yuhao Ma, Pedro A. Duarte, Cole Edwards, Ran Zhuo, Jie Zeng, Yiwei Feng, Carmen L. Charlton, Jamil N. Kanji, Shawn Babiuk, Jie Chen
Summary: The COVID-19 pandemic presents challenges for existing diagnostic tools, so there is a need to develop portable devices for fast and accurate detection. An impedimetric immunosensor based on microelectrode array has been developed to detect SARS-CoV-2 antibodies. The sensor uses non-Faradaic impedance and has a sensitivity that can be enhanced by adjusting the ionic strength. It has an LOD of 0.4 BAU/ml and can detect anti-spike antibodies in COVID-19 patient sera within 1 hour.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Materials Science, Biomaterials
Gulcin Bolat, Oznur Akbal Vural, Yesim Tugce Yaman, Serdar Abaci
Summary: Polydopamine nanoparticles (PDA NPs) have shown great potential for biomedical systems, with the ability to be used as a suitable material for functionalizing electrode surfaces for cell detection. The developed cytosensor exhibits good biocompatibility and sensitivity, making it a promising liquid biopsy method for early cancer detection.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Chemistry, Analytical
Ayman H. Kamel, Nashwa H. Ashmawy, Teraze A. Youssef, Mostafa Elnakib, Heba Abd El-Naby, Hisham S. M. Abd-Rabboh
Summary: This paper presents two electrochemical methods for albumin targeting using potentiometric and impedimetric transductions. The sensors are constructed with screen-printed platforms and incorporate ion-to-electron multi-walled carbon nanotubes transducer. These sensors demonstrate good detection performance and selectivity, and are characterized by simple construction, high sensitivity, fast response time, single-use, and cost-effectiveness. The methods have been successfully applied to albumin assessment in different biological fluids.
Article
Multidisciplinary Sciences
Mohammadali Sharifshazileh, Karla Burelo, Johannes Sarnthein, Giacomo Indiveri
Summary: The study introduces an embedded device that can process intracranial EEG from epilepsy patients to detect High Frequency Oscillations in real-time. By utilizing mixed-signal neuromorphic circuits, a neural network processing system achieves state-of-the-art accuracy, sensitivity, and specificity in HFO detection.
NATURE COMMUNICATIONS
(2021)
Article
Electrochemistry
Laia Anvari, Sayed Mehdi Ghoreishi, Kamyar Khoshnevisan, Mohammad Reza Ganjali, Farnoush Faridbod
Summary: Progress in highly sensitive analytical methods for illicit drugs poses serious challenges. Electrochemical aptamer-based sensors are considered potent analytical tools due to their portability, quick response, sensitivity, and simplicity. In this study, a novel aptasensor using electrochemical impedance spectroscopy (EIS) was presented for the determination of methamphetamine (METH). The aptasensor exhibited excellent repeatability, selectivity, and the ability to effectively detect METH in spiked urine samples.
JOURNAL OF APPLIED ELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Debabrata Mishra, Neeraj Bangruwa, Manish Srivastava
Summary: In this work, the authors demonstrate a label-free electrochemical impedimetric detection method for radiation-induced DNA damage using chiral-induced spin selectivity (CISS) and electrons' spin as a novel sensing tool. They prepared self-assembled monolayers (SAMs) of short ds-DNA on arrays of multilayer thin film devices and exposed them to UVC radiation to induce structural perturbations in DNA. The detection of DNA damage was achieved by recording spin-dependent electrochemical impedimetric spectra, taking into account the correlation between charge-transfer resistance (Rct) and the spin selectivity of electrons through DNA. The authors achieved a limit of detection of 10 picomolar concentrations of DNA, and the method showed robustness against electronic signal perturbation.
Article
Electrochemistry
Alnilan Lobato, Maja Subic, Tea Romih, Lea Zibret, Dino Metarapi, Mojca Bencina, Roman Jerala, Kristijan Vidovic, Samo B. Hocevar, Nikola Tasic
Summary: This paper presents a simple, disposable, sensitive, and selective label-free sensor for impedimetric detection of the SARS-CoV-2 virus Spike S1 protein. The immunosensor exhibited an excellent linear impedimetric response for the protein in clinically-relevant range and low limits of detection in both phosphate-buffered saline and artificial nasopharyngeal fluid.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Cigdem Gozde Aslan, Hilmiye Deniz Ertugrul Uygun, Zihni Onur Uygun, Nalan Gulsen Unal, Yasemin Akcay
Summary: This study introduces an innovative approach to diagnosing Crohn's disease using a rapid and precise biosensor system as an alternative to conventional detection techniques. The biosensor demonstrates its capability to reliably measure calprotectin levels in real samples.
Article
Chemistry, Analytical
Hongfei Gao, Dandan Cui, Shanshan Zhai, Yao Yang, Yuhua Wu, Xiaohong Yan, Gang Wu
Summary: A label-free electrochemical impedimetric biosensor based on gold carbon dots (GCDs) modified screen-printed carbon electrode was developed for the detection of GM soybean. The biosensor showed high sensitivity, specificity, and portability, and successfully detected target DNA with high specificity.
Article
Biophysics
Diana Seidel, Rebecca Rothe, Mandy Kirsten, Heinz-Georg Jahnke, Konstantin Dumann, Mirjana Ziemer, Jan-Christoph Simon, Andrea A. Robitzki
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Biophysics
Heinz-Georg Jahnke, Sabine Schmidt, Ronny Frank, Winnie Weigel, Christoph Proennecke, Andrea A. Robitzki
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Biochemistry & Molecular Biology
Alice Abend, Chelsie Steele, Sabine Schmidt, Ronny Frank, Heinz-Georg Jahnke, Mareike Zink
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Multidisciplinary
Christian Sonnendecker, Juliane Oeser, P. Konstantin Richter, Patrick Hille, Ziyue Zhao, Cornelius Fischer, Holger Lippold, Paula Blazquez-Sanchez, Felipe Engelberger, Cesar A. Ramirez-Sarmiento, Thorsten Oeser, Yuliia Lihanova, Ronny Frank, Heinz-Georg Jahnke, Susan Billig, Bernd Abel, Norbert Straeter, Joerg Matysik, Wolfgang Zimmermann
Summary: Enzyme-based hydrolysis is a promising strategy for sustainable recycling of plastics. A study found that the enzyme PHL7 can completely degrade plastics and the products can be used to synthesize new plastics.
Article
Biochemistry & Molecular Biology
Alice Abend, Chelsie Steele, Heinz-Georg Jahnke, Mareike Zink
Summary: Cell-material interactions are crucial for biomedical applications, impacting cell survival and behavior both in vitro and in vivo. Neurons and glial cells exhibit different adhesion and spreading characteristics on various culture materials, with TiN and nanocolumnar TiN supporting better neuronal growth. The designed nanocolumnar TiN also shows potential for building miniaturized microelectrode arrays for impedance spectroscopy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Sabine Schmidt, Tobias Haensch, Ronny Frank, Heinz-Georg Jahnke, Andrea A. Robitzki
Summary: In this study, a silicon nitride (SiN) passivation was established by physical vapor deposition (PVD) and evaluated for impedance spectroscopy-based monitoring of cells. The findings showed that SiN is a suitable alternative passivation, with significantly higher cell signals observed on microelectrodes larger than 50 μm in diameter. Additionally, the SiN passivation layer demonstrated high compatibility for bonding with PEGDA and PDMS microfluidic structures without any leakage.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Physical
Ronny Frank, Dana Krinke, Christian Sonnendecker, Wolfgang Zimmermann, Heinz-Georg Jahnke
Summary: The Earth has entered the Anthropocene, characterized by widespread environmental pollution from plastics like PET. There is a pressing need for ecofriendly and cost-effective solutions for plastic recycling and reuse. By altering the dielectric properties of PET films, a monitoring system has been developed using impedance spectroscopy to analyze the enzymatic hydrolysis process of PET films with polyester hydrolases.
Article
Biotechnology & Applied Microbiology
Anne Kuchenbuch, Ronny Frank, Jose Vazquez Ramos, Heinz-Georg Jahnke, Falk Harnisch
Summary: This study introduces a new electrochemical microwell plate for investigating the electrochemical and metabolic properties of electroactive microorganisms, allowing for experiments in both pure culture and co-culture modes. Results show that different electron donors have an impact on current density and coulombic efficiency, and the microbial electrochemical performance is correlated with the shift in microbial relative abundance.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Sabine Schmidt, Ronny Frank, Dana Krinke, Heinz-Georg Jahnke, Andrea A. Robitzki
Summary: Microelectrode arrays (MEA) are widely used for bioelectronic monitoring. In this study, we explored the use of a polymer-based substrate, PMMA, as an alternative to glass substrates. After optimizing the structuring process, we achieved a comparable resolution and integrated multiple vias into the substrate for easy contacting. We validated the performance of our PMMA MEA through different bioelectronic monitoring techniques.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Franziska D. Zitzmann, Sabine Schmidt, Max Naumann, Detlev Belder, Heinz-Georg Jahnke, Andrea A. Robitzki
Summary: Three-dimensional cell models are preferred for in vitro studies as they closely resemble the native in vivo situation. To meet the demand for noninvasive and label-free methods for analyzing three-dimensional cultures, impedance spectroscopy combined with microcavity arrays (MCAs) is desirable. In this study, we used selective laser etching to fabricate microcavities in fused silica and borosilicate glass, overcoming the limitations of the previous MCA. Our SLE-fabricated MCAs with variable base and central cone electrode improve the bioelectronic analyses of cellular changes in heterogeneous 3D models.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Ronny Frank, Jose Vazquez Ramos, Ronny Azendorf, Christoph Proennecke, Sabine Schmidt, Heinz-Georg Jahnke, Andrea A. Robitzki
Summary: Simultaneous optical and electrochemical measurements are advantageous for biosensor technology. Researchers have developed an electrochemical-optical 96-well microplate to track the bioelectrocatalysis process in real-time. The combination of fluorometric and chronoamperometric measurements allows for unbiased quantitative determination of enzymatic products and monitoring of enzyme activity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Dermatology
Katrin Rietscher, Heinz-Georg Jahnke, Matthias Ruebsam, Eric W. Lin, Cristina Has, M. Bishr Omary, Carien M. Niessen, Thomas M. Magin
Summary: This study demonstrates that the multikinase inhibitor PKC412 can reduce keratin aggregation in patient-derived EBS keratinocytes and restore intercellular adhesion. The findings provide a proof of concept for the repurposing of existing drugs for the targeted treatment of EBS and pave the way for clinical trials using PKC412 in EBS patients.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tobias Haensch, Franziska D. Zitzmann, Heinz-Georg Jahnke, Maximilian E. Blaha, Erik Paternoga, Kirsten Zeitler, Detlev Belder, Andrea A. Robitzki
Summary: This article introduces the miniaturization and integration of chemical reactions into fluidic systems to reduce solvent and reactant usage while increasing synthesis efficiency. Real-time, label-free monitoring is achieved through electrochemical impedance spectroscopy. The optimization of electrode structures in microfluidic channels allows for sensitive detection and quantification of compounds like propranolol. The integrated electrodes also enable the monitoring and control of fluidic flows for purification processes during free-flow electrophoresis.
Article
Materials Science, Biomaterials
Alice Abend, Chelsie Steele, Sabine Schmidt, Ronny Frank, Heinz-Georg Jahnke, Mareike Zink
Summary: Lab-on-a-chip devices offer advantages in studying biological cells, but realistic cell organization is important. A co-culture system of neuron-like and glial-like cells can model different brain regions. Nanocolumnar TiN has improved properties for biocompatibility and neural recording.
BIOMATERIALS SCIENCE
(2022)
Article
Biochemical Research Methods
Ronny Frank, Christoph Proennecke, Ronny Azendorf, Heinz-Georg Jahnke, Annette G. Beck-Sickinger, Andrea A. Robitzki