Journal
LAB ON A CHIP
Volume 8, Issue 10, Pages 1658-1663Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b805774e
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Funding
- National Natural Science Foundations of China [20675012, 20635010, 20725517]
- National Basic Research Program of China [2007CB714503]
- Ministry of Education, Dalian University of Technology [JMTZ200602]
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A miniaturized flow injection analysis (FIA) system integrating a micropump on a microfluidic chip based on capillary and evaporation effects was developed. The pump was made by fixing a filter paper plug with a vent tube at the channel end, it requires no peripheral equipment and provides steady flow in the mu l min(-1) range for FIA operation. Valve-free sample injection was achieved at nanolitre level using an array of slotted vials. The practical applicability of the system was demonstrated by DNA assay with laser-induced fluorescence (LIF) detection. A precision of 1.6% RSD (10.0 ng mu l(-1), n = 15) was achieved with a sampling throughput of 76 h(-1) and sample consumption of 95 nl.
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