Article
Biophysics
Wei-Ting Chen, Ping-Yeh Chiu, Chien-Fu Chen
Summary: In recent years, there has been an increasing demand for rapid, sensitive, and simple methods for diagnosing DNA due to the rise in infectious disease variations. This study introduces a flash signal amplification method combined with electrochemical detection for PCR-free tuberculosis molecular diagnosis. By utilizing the properties of butanol and water, we were able to concentrate the necessary components for DNA detection quickly, reducing reaction time. The developed method shows high sensitivity and specificity, capable of detecting low levels of DNA targets and is successfully applied in tuberculosis diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Physics, Applied
Xun Liu, Doudou Ma, Yapeng Yuan, Tao Tang, Yoichiroh Hosokawa, Yaxiaer Yalikun
Summary: This study investigates the use of passive pinched flow fractionation to achieve highly accurate separation of droplets with different sizes. Experimental and simulation results show that a suitable sheath flow rate ensures stable droplet separation. These findings serve as a valuable guide for designing passive droplet separation methods for various types of droplet-based cell sorting systems.
APPLIED PHYSICS EXPRESS
(2023)
Article
Chemistry, Analytical
Xiaolong Li, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: This study describes an amplified and highly sensitive fluorescent strategy based on CHA for detecting SNPs in the K-ras gene, with substantial discrimination capability. This method has significant implications in drug design and disease diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Ling Zhi Wu, Yuan Ye, Zhi Xuan Wang, Die Ma, Li Li, Guo Hao Xi, Bi Qing Bao, Li Xing Weng
Summary: A novel SNP detection assay has been developed for single-nucleotide discrimination using a nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The study successfully detected the SNP of p53 gene mutation in gastric cancer at femtomolar concentration, offering high sensitivity and spatial resolution for potential applications in early diagnosis and disease prevention.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Satoru Nagatoishi, Toshinobu Toyoshima, Kotohiro Furukawa, Kouhei Tsumoto
Summary: This study introduces a new method called Pinched-Flow Fractionation (PFF)-Coulter for the quantitative analysis of submicron-sized particles. The method combines PFF and Coulter techniques to separate and detect particles, allowing for more accurate particle analysis.
ANALYTICAL BIOCHEMISTRY
(2023)
Review
Engineering, Environmental
Seyed Samad Hosseini, Asiyeh Jebelli, Somayeh Vandghanooni, Ali Jahanban-Esfahlan, Behzad Baradaran, Mohammad Amini, Negar Bidar, Miguel de la Guardia, Ahad Mokhtarzadeh, Morteza Eskandani
Summary: Single nucleotide polymorphisms (SNPs) are the main cause of individual variability and are associated with various diseases. The healthcare industry requires more advanced technologies to detect SNPs. This review focuses on novel SNP biosensors based on electrochemical, optical, and piezoelectric analysis, and explores future trends in sensing.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Karl Normak, Marcell Papp, Michael Ullmann, Carolina Paganini, Mauro Manno, Antonella Bongiovanni, Paolo Bergese, Paolo Arosio
Summary: This study demonstrates that liquid chromatography combined with multi-angle light scattering (MALS) and fluorescence detection can provide multiparametric characterization of extracellular vesicles (EVs) in one single apparatus. The method allows for analysis of EV concentration, average diameter, protein amount to particle number ratio, presence of surface markers and lipids, EV shape, and sample purity. It is fully automatable and can be applied to both crude and purified samples with limited sample handling and data analysis time.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Daniel Papp, Thanya Rukkijakan, Daria Lebedeva, Tommy Nylander, Margareta Sandahl, Joseph S. M. Samec, Charlotta Turner
Summary: A novel method for universal quantification of lignin dimers based on supercritical fluid chromatography with charged aerosol detection (CAD) was developed and applied to quantify lignin dimers in an oil obtained by reductive catalytic fractionation (RCF) of birch sawdust. The applicability of linear regression and quadratic calibration curves for quantification was evaluated and found to be equally appropriate. The response factors of lignin dimers were compared and showed little variation, enabling the use of a single calibrant for these compounds. The importance of chromatographic peak resolution in CAD was stressed and addressed using a digital peak sharpening technique.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Daniel Papp, Thanya Rukkijakan, Daria Lebedeva, Tommy Nylander, Margareta Sandahl, Joseph S. M. Samec, Charlotta Turner
Summary: The increased interest in using lignin as a feedstock to produce aromatic compounds requires advanced chemical analysis methods for lignin characterization. This study presents a novel method for quantifying lignin dimers using supercritical fluid chromatography with charged aerosol detection. The method was validated using synthesized lignin dimers and found to be effective for quantification. The study also emphasizes the importance of chromatographic peak resolution and introduces a digital peak sharpening technique to address it.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jiaoyun Xia, Tong Xu, Jing Qing, Lihua Wang, Junlong Tang
Summary: The study introduced a novel biosensor based on the quenching effect of fluorescence-embedded SYBR Green I (SG) dye and graphene oxide (GO) for detecting single nucleotide polymorphisms (SNPs), which is crucial for early disease diagnosis and drug rational use. By optimizing experimental parameters, the sensor achieved high SNP discrimination and differentiation under optimal conditions, making it a low-cost, label-free, and simple operation method with high SNP resolution at room temperature.
FRONTIERS IN CHEMISTRY
(2021)
Review
Cell Biology
Farbod Bahreini, Elham Rayzan, Nima Rezaei
Summary: Breast cancer, a multifactorial disease, may have an increased risk due to alterations in microRNA sequences. miR-SNPs are potential biomarkers for early detection of breast cancer.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Concetta Scimone, Luigi Donato, Simona Alibrandi, Concetta Alafaci, Angela D'Ascola, Sergio Vinci, Rosalia D'Angelo, Antonina Sidoti
Summary: This study reveals the importance of epitranscriptomic modifications in gene expression regulation and suggests the involvement of altered epitranscriptome profile in the development of CCM. These findings provide new insights for further investigation into the pathogenesis of CCM.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Guralamatta Siddappa Ravi Kumara, Young Jun Seo
Summary: We developed a direct arylated oligonucleotide based molecular rotor for distinguishing perfect matched DNA sequences from one base mismatched sequences. This rotor showed high sensitivity and could be used to detect local dynamics of nucleic acids, providing a simple and cost-effective method for preparing SNP probes.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Chao Zhang, Tingting Zheng, Qian Ma, Linlin Yang, Mingzhi Zhang, Junyan Wang, Xiaoyan Teng, Yanyan Miao, Hsiao-chu Lin, Yang Yang, Da Han
Summary: Analyzing SNP combinations is crucial for research and clinical applications. DNA-based molecular computing has shown promise in sensing and analyzing molecular information. This study presents a DNA computational scheme that can sense multiple SNPs and perform logical analysis for clinical outcomes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Engineering, Chemical
Ali Amani, Amir Shamloo, Pouyan Vatani, Sina Ebrahimi
Summary: Microfluidic experiments have been widely used in medical sciences and engineering, and this study investigated the focusing and separation of particles with different sizes and densities using inertial microfluidic devices. The simulation results showed that the divergent geometry had better performance in separating and focusing particles compared to the convergent geometry.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Nanoscience & Nanotechnology
Mozhdeh Ghani, Arto Heiskanen, Janko Kajtez, Babak Rezaei, Niels Bent Larsen, Peter Thomsen, Anders Kristensen, Airidas Zukauskas, Martin Alm, Jenny Emneus
Summary: This study presents a novel reversible switchable UV-triggered drug delivery system based on interpenetrating polymer networks, utilizing photo-responsive IPNs to provide a new concept for controlled drug release. The research investigates how copolymer composition and drug lipophilicity affect the release profile, and develops a thermodynamic model to optimize the polymer composition for efficient light-triggered drug release. The results demonstrate excellent light-triggered release for specific drugs and highlight the potential of the developed IPNs in drug delivery applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Nanoscience & Nanotechnology
Soren Raza, Anders Kristensen
Summary: The introduction of resonant dielectric nanomaterials has opened up a new avenue for concentrating and manipulating light on the nanoscale, leading to new opportunities in Raman spectroscopy. Through dielectric resonances, it is possible to engineer the optical near field inside and outside nanostructures, achieving Raman enhancement. Research also investigates the impact of high-refractive-index dielectric materials on Raman spectroscopy.
Article
Physics, Applied
Xiaolong Zhu, Ilja Czolkos, Alicia Johansson, Theodor Nielsen, Anders Kristensen
Summary: This work presents a comprehensive solution for the design of plasmonic color elements suitable for lithography and production grade manufacture of plasmonic color metasurfaces on plastic substrates. The hybrid aluminum disk-hole structures with controlled multiple resonances enable plasmonic color generation across the visible spectrum with larger sized nanostructures, which can be defined by 248nm DUV lithography as a cost-efficient and high throughput alternative to electron beam lithography.
APPLIED PHYSICS LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Xiaolong Zhu, Jacob Engelberg, Sergei Remennik, Binbin Zhou, Jonas Nyvold Pedersen, Peter Uhd Jepsen, Uriel Levy, Anders Kristensen
Summary: This work presents a cost-effective and lithography-free method for printing optical metasurfaces. By utilizing resonant absorption of laser light in an optical cavity formed by a multilayer structure, nearly perfect light absorption is achieved through interferometric control. This approach demonstrates reliability, upscaling, and subwavelength resolution in creating metasurfaces for structural colors, optical holograms, and diffractive optical elements.
Article
Multidisciplinary Sciences
Arianna Toppi, Martin Dufva
Summary: A new method for fabricating microdroplet array (MDA) substrates independently from cleanroom processes was developed. A low-cost in-house built system for photopatterning a negative resist was assembled to produce stable and high-quality MDAs. The feasibility for carrying out digital assays was demonstrated.
Article
Multidisciplinary Sciences
Asli Aybike Dogan, Martin Dufva
Summary: There is a high demand for complex cell cultures in various fields, with Transwell inserts being popular due to their commercial availability, ease of use, and versatility. Customizing inserts can offer potential benefits in terms of layers, height, and membrane composition, allowing for tailored experiments and improved culture formation.
SCIENTIFIC REPORTS
(2022)
Correction
Multidisciplinary Sciences
Asli Aybike Dogan, Martin Dufva
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Maria Niora, Mathilde Hauge Lerche, Martin Dufva, Kirstine Berg-Sorensen
Summary: Fluorescent nanodiamonds (FNDs) with negative nitrogen-vacancy (NV-) defect centers are ideal probes for biosensing applications and can potentially serve as biomarkers for cell differentiation. The uptake of FNDs by THP-1 monocytes and monocyte-derived M0-macrophages is studied, revealing differing FND-cell interactions and time-dependent uptake. FND uptake allows for distinction between cell subtypes based on phagocytic capacity, and FNDs demonstrate effective cell labeling and have potential for sensing biological processes involving cell differentiation.
Article
Engineering, Biomedical
Murat Serhatlioglu, Emil Alstrup Jensen, Maria Niora, Anne Todsen Hansen, Christian Friberg Nielsen, Michelle Maria Theresia Jansman, Leticia Hosta-Rigau, Morten Hanefeld Dziegiel, Kirstine Berg-Sorensen, Ian David Hickson, Anders Kristensen
Summary: A compact microfluidic flow cytometer with viscoelastic flow focusing and fiber optical interface is demonstrated. The device allows for easy operation and interchangeable capillaries to achieve single-train particle focusing for a wide range of particle sizes. The system is integrated with optical imaging and other optofluidic modalities and achieves a high throughput of 3500 events s(-1).
ADVANCED NANOBIOMED RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Asli Aybike Dogan, Martin Dufva
Summary: This study introduces a customized hanging insert for cultivating Caco-2 cells on hydrogel membranes under flow conditions. The device consists of an apical channel-like chamber for cell culture with shear force and a basolateral chamber that is mixed when rocked. The experiment investigates the impact of seeding density and flow on the growth of the cells. The results show that low seeding density and flow promote the formation of three-dimensional structures and improve barrier function.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
Martin Dufva
Summary: Out of 2828 screened articles, only 146 contained controls and quantified data for flow cell culture. Analysis of these articles showed that many biomarkers in all cell types were unaffected by flow, while only specific biomarkers responded strongly to flow.
SCIENTIFIC REPORTS
(2023)
Article
Materials Science, Multidisciplinary
Emil Alstrup Jensen, Murat Serhatlioglu, Cihan Uyanik, Anne Todsen Hansen, Sadasivan Puthusserypady, Morten Hanefeld Dziegiel, Anders Kristensen
Summary: Label-free blood typing using Raman spectroscopy and artificial intelligence was demonstrated in this study. Training an AI model on a dataset of Raman spectra of blood samples allowed classification of ABO blood group, erythrocyte antigens, platelet antigens, antibody titers, and ABH-secretor status. The results show promising potential for future applications in transfusion medicine and blood banking.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Proceedings Paper
Engineering, Multidisciplinary
Murat Serhatlioglu, Emil Alstrup Jensen, Maria Niora, Anne Todsen Hansen, Christian Friberg Nielsen, Michelle Maria Theresia Jansman, Leticia Hosta-Rigau, Morten Hanefeld Dziegiel, Kirstine Berg-Sorensen, Ian D. Hickson, Anders Kristensen
Summary: Flow cytometry (FC) is a pivotal tool for studying the physical and chemical properties of particles. This study presents a miniaturized optical capillary FC device using the viscoelastic focusing technique. The device can collect and analyze light signals in real-time and can be used for both flow cytometry analysis and microscopy imaging. The device has been successfully used for particle measurements and can be combined with other tools for extended applications.
OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION XIX
(2022)
Proceedings Paper
Engineering, Multidisciplinary
Emil Alstrup Jensen, Murat Serhatlioglu, Airidas Zukauskas, Cihan Uyanik, Anne Todsen Hansen, Sadasivan Puthusserypady, Morten Hanefeld Dziegiel, Anders Kristensen
Summary: This study presents a multivariate analysis of human-blood samples for ABO blood typing using Raman spectroscopy and support vector machine (SVM) classification. It addresses the issues of expensive and time-consuming traditional ABO blood typing methods, demonstrating the great potential of the developed system for future blood typing applications.
OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION XIX
(2022)
Article
Engineering, Biomedical
Salime Bazban-Shotorbani, Felicity Gavins, Krishna Kant, Martin Dufva, Nazila Kamaly
Summary: A dysfunctional endothelium is a hallmark of many diseases, including atherosclerosis. Researchers found that size plays a dominant role in nanoparticle targeting, with nanoparticles in the range of 30-60nm showing increased targeting to dysfunctional endothelium using a biomicrofluidic model. Additionally, treating dysfunctional endothelium with Annexin A1 resulted in restoration of adherens junctions and normalization of barrier integrity.
ADVANCED NANOBIOMED RESEARCH
(2022)