Article
Cell Biology
Bjoern Klabunde, Andre Wesener, Wilhelm Bertrams, Stephan Ringshandl, Luke D. Halder, Evelyn Vollmeister, Bernd Schmeck, Birke J. Benedikter
Summary: Streptococcus pneumoniae infection disrupts the Golgi apparatus through H2O2 production, inhibiting anti-infective mechanisms and promoting bacterial attachment and replication.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Medicine, Research & Experimental
Jian Zhou, Rui Li, Qinhui Liu, Jinhang Zhang, Hui Huang, Cuiyuan Huang, Guorong Zhang, Yingnan Zhao, Tong Wu, Qin Tang, Ya Huang, Zijing Zhang, Yanping Li, Jinhan He
Summary: The enzyme 5-lipoxygenase (5-LO) promotes renal fibrosis by inducing epithelial-mesenchymal transition (EMT) in kidney cells. Deletion or inhibition of 5-LO can reduce renal fibrosis.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Nutrition & Dietetics
Kai Shan, Ninghan Feng, Doudou Zhu, Hongyan Qu, Guoling Fu, Jiaqi Li, Jing Cui, Heyan Chen, Rong Wang, Yumin Qi, Yong Q. Chen
Summary: Fatty acid unsaturation is positively correlated with the sensitivity of ferroptosis. Docosahexaenoic acid (DHA) effectively promotes ferroptosis-mediated tumor killing by increasing intracellular lipid peroxidation, and both ALOX5 dependent and independent pathways may be involved in this process.
EUROPEAN JOURNAL OF NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Wen-Sheng Wu, Chin-Hsien Ling, Ming-Che Lee, Chuan-Chu Cheng, Rui-Fang Chen, Chen-Fang Lin, Ren-In You, Yen-Cheng Chen
Summary: Cholangiocarcinoma (CCA) is often associated with the deregulation of oncogenic signaling molecules, such as receptor tyrosine kinases (RTKs). Over-expression of hydrogen peroxide inducible clone-5 (Hic-5) and over-activation of Src, AKT, and JNK have been observed in CCA with metastatic potential. Positive cross-talk between Hic-5 and Src triggers AKT activation. Depletion of Hic-5 and inhibition of Src effectively suppresses HuCCT1 cell migration.
Article
Chemistry, Applied
Lijuan Zhu, Wanfeng Hu, Ayesha Murtaza, Aamir Iqbal, Mengjie Kong, Jiao Zhang, Jiaxing Li, Xiaoyun Xu, Siyi Pan
Summary: In this study, high pressure CO2 treatment was found to inhibit browning reactions in fresh-cut Chinese water chestnut. This was achieved by regulating ROS and membrane lipid metabolism, reducing enzymatic oxidation of phenolic compounds and biosynthesis of flavonoids in surface tissue, and enhancing antioxidant activity in inner tissue.
Article
Plant Sciences
Suhyeon Lee, Hyo Jun Won, Seunghyun Ban, Yun Ji Park, Sang Min Kim, Hyoung Seok Kim, Jaeyoung Choi, Ho-Youn Kim, Jae Hoon Lee, Je Hyeong Jung
Summary: This study comprehensively analyzed the transcriptome data and flavonoids contents from four different tissues of Artemisia argyi for the first time, and identified candidate genes involved in the biosynthesis of eupatilin and jaceosidin. A total of 7,265 differentially expressed genes were found, among which 153 were annotated as flavonoid-related genes. Furthermore, eight putative flavone-6-hydroxylase genes and five O-methyltransferases genes were identified. These findings pave the way for modifying and mass-producing pharmacologically important polymethoxy flavonoids through genetic engineering and synthetic biological approaches.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Anna Och, Marta Olech, Kamil Bak, Sebastian Kanak, Anna Cwener, Marek Ciesla, Renata Nowak
Summary: Berberis vulgaris L. is being studied for its antioxidant and chemopreventive properties, particularly in relation to its fruits. This study aimed to investigate the effects of extracts from different parts of the plant on antioxidant and LOX inhibitory activity, as well as to analyze the presence and quantity of phenolic acids and flavonoids. The research found that the leaves had the highest polyphenol content and antioxidant activity, while the stem had the lowest values.
Article
Biochemistry & Molecular Biology
Gregory E. Conner
Summary: The redox status of cells is crucial for cellular activities; H2O2 metabolism regulates the redox status by modifying protein cysteine oxidation; Calcium-stimulated epithelial Duox H2O2 synthesis is transient and prevents harmful changes in redox tone following continuous stimulation.
Article
Cell Biology
Patricia M. Silva, Ines da Silva, Maria J. Sarmento, Itala C. Silva, Filomena A. Carvalho, Graca Soveral, Nuno C. Santos
Summary: This study evaluated the involvement of AQP3 and AQP5 in cell biomechanical properties, cell-cell adhesion, and cell migration. The results showed that silencing AQP3 and AQP5 could slow down cell migration and wound healing, increase membrane fluidity, and decrease cell stiffness and cell-cell adhesion.
Article
Biochemistry & Molecular Biology
Liping Chen, Shihao Zhu, Tianyuan Liu, Xuan Zhao, Tao Xiang, Xiao Hu, Chen Wu, Dongxin Lin
Summary: This study unravels the role of EFNB1-EPHB4 interaction in triggering epithelial-mesenchymal transition (EMT) and cell proliferation in epithelial cancer formation and progression.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Cell Biology
Divya Choudhary, Valentine Lagage, Kevin R. Foster, Stephan Uphoff
Summary: Genetically identical bacterial cells often exhibit different phenotypes, which is commonly explained as a bet-hedging strategy against unpredictable environmental threats. However, this study reveals that phenotypic heterogeneity in Escherichia coli's stress response to hydrogen peroxide stress has a fundamentally different basis. Through microfluidic experiments, it is found that the heterogeneity arises from precise and rapid feedback between individual cells and their immediate environment. Furthermore, cell-cell interactions play a crucial role in shielding each other from hydrogen peroxide, resulting in a collective phenotype that protects a significant portion of the population.
Article
Biochemistry & Molecular Biology
Yubo Sun, Xiangrong Tian, Hao Wu, Xinchang Hao, Baowei Gao, Hongyan Zhang, Juntao Feng, Lirong Han
Summary: GP-1 is a glycoprotein elicitor found in Streptomyces kanasensis zx01 with antiviral activity, and it induces programmed cell death (PCD) in tobacco suspension cells, modulated by hydrogen peroxide (H2O2) and participating in biologically relevant signaling in plant cells.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hirokazu Komatsu, Nadiia Velychkivska, Anastasiia B. Shatan, Yutaka Shindo, Kotaro Oka, Katsuhiko Ariga, Jonathan P. Hill, Jan Labuta
Summary: Nicotine adenine dinucleotide derivatives NADH and NADPH are involved in energy and electron transport in cells. The fluorescent ubiquinone-rhodol probe Q-Rh is used to monitor NADPH activation, and the addition of an Ir-III-complex promotes the activation. Experiments show that the reduction rate of Q-Rh is increased in HeLa cells especially with the addition of the Ir-III-complex promoter, involving the nonspecific activation of other intracellular reducing agents.
Article
Chemistry, Analytical
Fanny Girard-Sahun, Pauline Lefrancois, Vasilica Badets, Stephane Arbault, Franck Clement
Summary: This study aims to detect the in situ production of reactive oxygen and nitrogen species (RONS), specifically superoxide anion (O-2(center dot-)), in aqueous buffer solutions exposed to cold atmospheric plasmas (CAPs). The production and accumulation of RONS in the liquids were found to depend on the plasma composition, with N-2-based shieldings providing the highest concentrations of superoxide. The study provides valuable insights into the interactions between CAPs and physiological solutions for biomedical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sopita Rattanopas, Albert Schulte, Somjai Teanphonkrang
Summary: In this study, effective amperometric sensors for cathodic hydrogen peroxide (H2O2) detection were produced and characterized, demonstrating good linearity and high sensitivity. The combination of PB/CNT layer and gelatin/zein layer in a sandwich-type sensor proved to be successful, providing accurate detection of H2O2 concentrations in model and real samples. The sensors are cost-effective, easy to make, and can be used for electrochemical H2O2 analyses in various applications.
ANALYTICAL CHEMISTRY
(2022)