Evaluation of the MicroScan NegCombo Panel Type 44 for Detection of Extended-Spectrum β-Lactamase among Clinical Isolates of Escherichia coli, Klebsiella species, and Proteus mirabilis

Background : Accurate and rapid detection of extended-spectrum beta-lactamases (ESBLs) is important in guiding proper antimicrobial therapy for infected patients. We evaluated the performance of MicroScan NegCombo Type 44 panel (Dade Behring, USA), which was developed to confirm ESBL-producing Enterobacteriaceae using ceftazidime/clavulanate and cefotaxime/clavulanate. Methods : From August 30 to September 20, 2007, 206 non-duplicate clinical isolates, including 106 Escherichia coli, 81 Klebsiella pneumoniae, 11 Klebsiella oxytoca, and 8 Proteus mirabilis were subcultured and tested with Type 32 and Type 44 panels. The results were compared with those of the CLSI phenotypic confirmatory test (CLSI-PCT) and disk approximation test (DAT). Isolates not susceptible to cefotetan or flagged as Possible ESBL, unable to interpret confirm test (Possible ESBL) on Type 44 panel were tested with boronic acid disks to confirm AmpC beta-lactamases (AmpC) production. Results : Of the 206 isolates tested, 44 (21.4%) produced ESBL by CLSI-PCT or DAT, including 27 E coli, 14 K. pneumoniae, 2 K. oxytoca, and 1 P. mirabilis. Thirty-eight isolates flagged as Confirmed ESBL on Type 44 panel were all confirmed as ESBL-producers. Of 14 K pneumoniae flagged as Possible ESBL, 6 were confirmed as ESBL and AmpC co-producers and 8 as AmpC-producers. Conclusions: Type 44 panel showed an excellent performance in detecting ESBL-producing E coli, Klebsiella spp., and P. mirabilis. When flagged as Confirmed ESBL, no other confirmatory test was necessary to report as ESBL; however, Possible ESBL required a differential test for AmpC production. (Korean J Lab Med 2009;29:3540)