Article
Food Science & Technology
Phanthipha Laosam, Worawan Panpipat, Manat Chaijan, Sittiruk Roytrakul, Sawanya Charoenlappanit, Atikorn Panya, Natthaporn Phonsatta, Ling-Zhi Cheong, Gorawit Yusakul
Summary: In this study, a porcine placenta hydrolysate (PPH) with a 30% degree of hydrolysis was fractionated using ultrafiltration (UF) to obtain the UF4 fraction. The UF4 fraction showed excellent antioxidant and enzyme inhibitory activities, as well as high stability. Additionally, the UF4 fraction was rich in hydrophobic amino acids. These findings suggest that the UF4 fraction isolated from crude PPH can be developed as a potential functional food ingredient.
FOOD AND BIOPROCESS TECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Jun-Hui Cheng, Xiao-Yu Zhang, Zhen Wang, Xia Zhang, Shi-Cheng Liu, Xiao-Yan Song, Yu-Zhong Zhang, Jun-Mei Ding, Xiu-Lan Chen, Fei Xu
Summary: The thermophilic TLP A69 has shown promising potential in preparing bioactive collagen peptides with antioxidative activity, laying a foundation for the high-valued utilization of bovine bone collagen through efficient hydrolysis processes.
Article
Chemistry, Multidisciplinary
Hana Derbel, Jihen Elleuch, Wael Mahfoudh, Philippe Michaud, Imen Fendri, Slim Abdelkafi
Summary: Bioactive peptides extracted from microalgae have gained significant attention recently due to their promising properties. In this study, proteins from the marine microalga Rhodomonas sp. were hydrolyzed into peptides using alcalase and pepsin. The biological activities of the protein preparations were evaluated and it was found that the crude protein exhibited higher antioxidant and anti-inflammatory activities compared to the peptides obtained after hydrolysis.
APPLIED SCIENCES-BASEL
(2023)
Article
Acoustics
Yuejing Hao, Lujuan Xing, Zixu Wang, Jiaming Cai, Fidel Toldra, Wangang Zhang
Summary: In this study, the effects of ultrasound-assisted enzymatic hydrolysis on the extraction of anti-inflammatory peptides from porcine bone collagen were investigated. It was found that ultrasound treatment increased the content of alpha-helix while decreased beta-chain and random coil, and promoted the generation of small molecular peptides. Ultrasound-assisted enzymatic hydrolysis improved the peptide content, enhanced ABTS+ radical scavenging and ferrous ion chelating ability. Peptides obtained through ultrasound-assisted enzymatic hydrolysis demonstrated significant anti-inflammatory activity, suppressing the release of interleukin-6 and tumor necrosis factor-alpha in lipopolysaccharide-induced RAW264.7 cells. Eight peptides with potential anti-inflammatory activities were selected through LC-MS/MS analysis, PeptideRanker, and molecular docking. Overall, ultrasound-assisted enzymatic hydrolysis proved to be an effective strategy for extracting bioactive peptides from porcine bone, and the anti-inflammatory regulation capacity of bone collagen sourced peptides was demonstrated for the first time.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Mengchu Gao, Xu Zhang, Yongxin Tian, Chunxiao Zhang, Biyu Peng
Summary: In this study, collagen hydrolytic activity of protease was detected using unlabeled native bovine hide powder, and the optimal conditions for the determination method were identified. The results showed distinct trends in collagenolytic activities compared to proteolytic activity assay methods based on different substrates, such as casein or dye-labeled hide powder. This method is considered more reasonable than traditional protease assay methods in various fields.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Food Science & Technology
Boo-Sik Jang, Mi-Jin Lee, Noh-Hee Jeong
Summary: The study examined the potential use of canola glycoprotein (CNG) in various fields by comparing its characteristics and physiological activities with commercially available carrot glycoprotein (CRG). CNG has higher protein content, lower molecular weight distribution, and stronger antioxidant activity compared to CRG.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Agriculture, Multidisciplinary
Jun-Hui Cheng, Wen-Xiao Zhao, Hai-Yan Cao, Zhen Wang, Yan Wang, Qi Sheng, Yin Chen, Peng Wang, Xiu-Lan Chen, Yu-Zhong Zhang
Summary: Collagen oligopeptides are widely used in various industries for their high bioactivities and bioavailability. A4095, the most abundant S8 protease secreted by Anoxybacillus caldiproteolyticus 1A02591, was characterized as a collagenolytic protease that can produce collagen oligopeptides by preferentially hydrolyzing the Y-Gly peptide bonds in denatured bovine bone collagen.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Kritsanaporn Tansathien, Phuvamin Suriyaamporn, Tanasait Ngawhirunpat, Praneet Opanasopit, Worranan Rangsimawong
Summary: The study investigates the potential of bioactive placenta for skin regeneration and develops dissolving microneedles loaded with bioactive extract for dermal delivery. The placental extracts contain high amounts of proteins, growth factors, and amino acids. They are non-toxic to skin cells and promote the proliferation of fibroblast cells. GP-loaded DMNs show the highest bioactivity and can serve as a novel approach for skin regeneration.
Article
Chemistry, Multidisciplinary
Xiaoyun Mo, Suwen Zhao, Jie Zhao, Yongjie Huang, Tao Li, Yongqiao Zhu, Gang Li, Yang Li, Hong Shan
Summary: Antimicrobial peptides (AMPs) have potential as anti-infective drugs, but their effectiveness is limited by short retention at the infection site and non-targeted uptake. Researchers have developed a strategy to overcome these limitations by immobilizing AMPs to damaged collagen, creating a reservoir for sustained release. This approach has shown enhanced antimicrobial efficacy and tissue repair in vitro and in vivo, offering a new avenue for antimicrobial treatments in a range of infected tissues.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Endocrinology & Metabolism
Yinglin Feng, Xia Chen, Huiqiao Wang, Xueping Chen, Zixin Lan, Pan Li, Yingshi Cao, Mian Liu, Jin Lv, Yun Chen, Yu Wang, Chao Sheng, Yingying Huang, Mei Zhong, Zhijian Wang, Xiaojing Yue, Liping Huang
Summary: The study revealed that human preeclamptic placenta exhibited significantly higher fibrosis in stem and terminal villi, characterized by collagen I deposition. Collagen I suppressed trophoblast proliferation and invasion by inhibiting ERK phosphorylation and WNT/beta-catenin signaling pathways.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ikhlas Laasri, Mohammed Bakkali, Laura Mejias, Amin Laglaoui
Summary: Marine organisms such as fish and shellfish contain collagen with useful properties that can be applied in various sectors. Over the past decade, research on collagen extraction from fish waste has gained attention. Acidic and enzymatic methods for collagen extraction are cost-effective and yield a high amount of collagen. Marine collagen is environmentally sustainable and has excellent biocompatibility, bioactivity, and low antigenicity. Additionally, collagen-derived peptides have potential antioxidant, antihypertensive, and antimicrobial activities. This review discusses the advancements in marine collagen extraction and detection methods as well as its diverse range of applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Umesh Patil, Khurshid Ahmed Baloch, Shivraj Hariram Nile, Jun Tae Kim, Soottawat Benjakul
Summary: The study aimed to purify trypsin from the pyloric caeca of Asian seabass and compare its proteolytic capability with commercial porcine trypsin. Trypsin was purified using ammonium sulphate precipitation and a soybean trypsin inhibitor column. The purified trypsin showed high stability and enzymatic activity, making it a potential alternative to commercial proteases. It also had a slightly lower hydrolysis capacity toward acid-soluble collagen compared to commercial trypsin.
Article
Engineering, Environmental
Hui Liu, Xiumin Li, Keyong Tang, Shufa Qin, Jie Liu
Summary: By using alkaline protease to remove hair and open collagen fiber-bundles after pre-swelling bovine hides with alkali, a green and high-efficiency ecological processing technology for preparing pure collagen fiber woven materials was designed. The cross-linked pure collagen material produced from this process has better fiber fullness, fleeciness, and grain surface smoothness compared to conventional methods. The pollutants load of wastewater from this process is significantly lower as well.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Jerrell Felim, Chun-Kai Chen, David Tsou, Hsiang-Ping Kuo, Zwe-Ling Kong
Summary: Marine collagen has potential therapeutic effects for treating osteoarthritis. Collagen and glycine can inhibit the production of proinflammatory cytokines, attenuating cartilage degradation, while collagen peptides promote cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Yifei Sun, Yuanmeng Cui, Ruhua Wang, Junrui Ma, Haili Sun, Lei Cheng, Rui Yang
Summary: Phycoerythrin (PE) was extracted from Porphyra haitanensis with a yield of 0.2% (w/w). The enzymatic hydrolysis of PE with bromelain resulted in the formation of phycoerythrin hydrolysates (PEH) (3-10 kDa) with a degree of hydrolysis (DH) of 11.57 ± 0.39% and improved antioxidant activity and stability. This study provides a potential scheme for overcoming the sensitivity of PE to thermal and light variations and facilitates the application of PEH as a natural colorant ingredient in food and pigment industries.