Review
Allergy
Alexandra F. Santos, Oral Alpan, Hans-Jurgen Hoffmann
Summary: The basophil activation test (BAT) measures the degree of degranulation of basophils following stimulation with allergen, and it can be used as a more specific diagnostic tool for allergic diseases. Clinical application of BAT requires clinical validation, quality assurance, and efforts are ongoing to establish a platform for laboratories to ensure reproducibility and reliability of results.
Article
Allergy
Nicolas Castano, Kaiser Chua, Abhinav Kaushik, Sungu Kim, Seth C. Cordts, Ceena D. Nafarzadegan, Grady H. Hofmann, Hana Seastedt, Jackson P. Schuetz, Diane Dunham, Ella S. Parsons, Mindy Tsai, Shu Cao, Manisha Desai, Sayantani B. Sindher, R. Sharon Chinthrajah, Stephen J. Galli, Kari C. Nadeau, Sindy K. Y. Tang
Summary: Avidin and CD63 have been found to be reliable basophil activation markers that enhance the identification of activated basophils and improve the classification accuracy of peanut allergy.
Review
Allergy
Julie Wang, David B. K. Golden, David A. Khan
Summary: An accurate diagnosis of IgE-mediated allergies is crucial for managing severe allergic reactions. However, currently available laboratory tests have limitations, especially when it comes to diagnosing drug allergies. Researchers are exploring alternative laboratory testing methods to improve diagnostic accuracy and predict treatment responses.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Medical Laboratory Technology
Theodore Kim, Jing Yu, Henry Li, Mark Scarupa, Richard L. Wasserman, Athena Economides, Martha White, Carla Ward, Atul Shah, Douglas Jones, Melinda Rathkopf, Kelly Frye, Ahmet Aybar, Shahrooz Shayegan, Benjamin Enav, Laura Ispas, Denise Loizou, David Fitzhugh, James Tracy, James Friedlander, Zachary Jacobs, Jonathan Matz, David Golden, Donald McNeil, William McCann, Christopher Copenhaver, Jeffrey Factor, Raavi Gupta, Oral Alpan, Matthew Plassmeyer, Soren Ulrik Sonder
Summary: This study found that CD63 remains a stable marker at Day 1 under temperature-controlled conditions, while the CD203c ratio significantly decreases at Day 1. Calculating the CD63/IgE ratio proves to be more stable than CD63 alone. The most stable readouts are the semi-quantitative results and the trajectory of the dose response curve. Ultimately, the stability of basophil activation assay can be extended to 1 day for samples stored at 18-25 degrees C and samples shipped under ambient conditions within the 2-37 degrees C range.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2021)
Review
Allergy
Patrizia Bonadonna, Peter Korosec, Francesca Nalin, David B. K. Golden
Summary: Diagnostic testing for insect venom allergy requires a detailed initial history to assess severity and predict future reactions. Venom skin tests and specific IgE measurement confirm the diagnosis but have limited predictive value. Testing for serum IgE to recombinant venom component allergens can distinguish true allergy from cross reactivity. Basophil activation tests and elevated tryptase levels are useful for detecting and predicting the severity of venom allergy. Bone marrow biopsy is the definitive tool for characterizing mast cell disorders in patients with insect sting allergy. (From J Allergy Clin Immunol Pract 2023;11:2024-31)
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Allergy
Hannah Jaumdally, Matthew Kwok, Zainab Jama, Rochelle Hesse-Lamptey, Richard McKendry, Oliver Galvez, Yvonne Daniel, Alexandra F. Santos
Summary: The basophil activation test (BAT) is a reliable test that can be transferred between laboratories, but different BAT methods are not interchangeable. BAT can be well integrated into the clinical decision-making process and reduces the need for oral food challenges (OFC).
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2022)
Review
Allergy
Didier G. Ebo, Chris H. Bridts, Christel H. Mertens, Vito Sabato
Summary: The major challenge in allergy diagnosis is the development of accessible and reliable diagnostics for predicting clinical outcomes after exposure to allergens. Flow-assisted analysis and the basophil activation test (BAT) show promise in meeting this requirement for various IgE-dependent allergies, but proper execution and interpretation are essential. Other uses of BAT include therapeutic monitoring and identifying allergenic recognition sites.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Allergy
Christine Y. Y. Wai, Nicki Y. H. Leung, Agnes S. Y. Leung, Yuki Shum, Patrick S. C. Leung, Ka Hou Chu, Yat Wah Kwan, Qun Ui Lee, Joshua S. C. Wong, Ivan C. S. Lam, Pui Fung Li, Kary J. Y. Xu, Cheuk Yin Lam, Jinlyu Sun, Gary W. K. Wong, Ting Fan Leung
Summary: This study compared diagnostic methods for shrimp allergy and found that BAT is more accurate than SPT and shrimp sIgE, with EXiLE being a good alternative to BAT. In addition, tropomyosin may not be the most important shrimp allergen in Chinese populations, suggesting a need for further investigation into other major allergens and diagnostic markers.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2021)
Article
Medicine, Research & Experimental
Ragha Suresh, Collin Dunnam, Dhananjay Vaidya, Robert A. Wood, Bruce S. Bochner, Donald W. MacGlashan Jr, Melanie C. Dispenza
Summary: In this study, acalabrutinib, a BTK inhibitor, showed significant efficacy in increasing the tolerance of patients with peanut allergy to peanut protein, with minimal adverse events. The findings support the need for larger, placebo-controlled trials.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Allergy
Kevin Heremans, Alessandro Toscano, Jessy Elst, Athina L. Van Gasse, Christel Mertens, Michiel Beyens, Marie -Line M. van der Poorten, Margo M. Hagendorens, Didier G. Ebo, Vito Sabato
Summary: Safe in vitro diagnostic methods are needed for the accurate diagnosis of amoxicillin allergy. The basophil activation test (BAT) has shown potential, but its clinical reliability is doubtful.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Immunology
Jingyang Li, Jinhong Wu, Haipei Liu, Li Hua, Quanhua Liu, Dingzhu Fang, Yi Chen, Ruoxu Ji, Jianhua Zhang, Wenwei Zhong
Summary: The study analyzed the differences in basophil activation test (BAT) results among children with recurrent wheezing episodes who had different asthma outcomes. Results showed that CD63-based BAT has potential clinical value for predicting asthma outcomes in young children with wheezing episodes.
Article
Allergy
Suzana Radulovic, Ru-Xin Foong, Irene Bartha, Andreina Marques-Mejias, Marta Krawiec, Matthew Kwok, Zainab Jama, Faye Harrison, Cristian Ricci, Gideon Lack, George Du Toit, Alexandra F. Santos
Summary: Identifying patients at risk of severe allergic reactions and/or low threshold of reactivity to staple foods like egg is crucial. A study found that blood serology and basophil activation test (BAT) could better predict the severity and threshold of allergic reactions to egg compared to the double-blind placebo-controlled food challenge (DBPCFC).
Article
Immunology
Yasmeen S. El Ansari, Cynthia Kanagaratham, Oliver T. Burton, Jenna V. Santos, Brianna-Marie A. Hollister, Owen L. Lewis, Harald Renz, Hans C. Oettgen
Summary: Mast cells and basophils play important roles in IgE-mediated hypersensitivity reactions. IgG and IgA antibodies can regulate the activation of these cells by activating or inhibiting receptors. This study found that IgA antibodies can bind to mast cells and basophils, and inhibit their activation to maintain immune homeostasis at mucosal sites.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Allergy
Michael R. Goldberg, Michael Y. Appel, Rachel Nega, Michael B. Levy, Naama Epstein-Rigbi, Liat Nachshon, Arnon Elizur
Summary: The study validated a diagnostic algorithm to reduce the need for oral food challenges and predict the severity of walnut/pecan allergies using SPT and BAT. BAT is associated with the severity of walnut/pecan allergies, and the algorithm decreased the need for oral food challenges.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2021)
Article
Allergy
Oliver Hemmings, Umar Niazi, Matthew Kwok, Louisa K. James, Gideon Lack, Alexandra F. Santos
Summary: The study showed that individuals with peanut allergy have stronger IgE characteristics compared to peanut-sensitized but tolerant individuals, with peanut SA and peanut diversity having the greatest influence on effector cell activation.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Engineering, Biomedical
Laura Huth, Sebastian Huth, Yvonne Marquardt, Philipp Winterhalder, Timm Steiner, Frank Hoelzle, Reinhard Gansel, Jens Malte Baron, Laurenz Schmitt
LASERS IN MEDICAL SCIENCE
(2021)
Article
Dermatology
Sebastian Huth, Yvonne Marquardt, Laura Huth, Laurenz Schmitt, Kirsten Prescher, Philipp Winterhalder, Timm Steiner, Frank Hoelzle, Michael Eble, Jens Malte Baron
Summary: The study found that aftercare treatment with dexpanthenol-containing ointment or liquid can completely restore the epidermal part of skin models after radiation, exhibiting anti-oxidative and anti-inflammatory effects, and positively influencing epidermal differentiation and structures.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Engineering, Biomedical
Sebastian Huth, Laura Huth, Yvonne Marquardt, Maria Cheremkhina, Ruth Heise, Jens Malte Baron
Summary: The study investigated the role of MMP-3 and its regulation by calcium pantothenate in wound healing after ablative fractional laser treatment. Results showed that aftercare treatment with calcium pantothenate accelerated wound closure in MMP-3 expressing models faster than in MMP-3 knockdown models. This study provides evidence for the first time that calcium pantothenate promotes wound healing at least partly via MMP-3.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Immunology
Ramona A. A. Eichkorn, Morna F. F. Schmidt, Elias Walter, Michael Hertl, Jens Malte Baron, Jens Waschke, Amir S. S. Yazdi
Summary: This study reveals the molecular mechanisms underlying auto-antibody-induced acantholysis in pemphigus vulgaris and identifies UVA as a cofactor for acantholysis. It demonstrates that UVA induces IL-1 secretion in keratinocytes and enhances the loss of epidermal adhesion mediated by anti-Dsg3 antibodies. These findings suggest that UVA plays a caspase-dependent exogenous role in blister formation in pemphigus vulgaris.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Dermatology
Uwe Paasch, Miriam Zidane, Jens Malte Baron, Thorsten Bund, Hans-Joachim Cappius, Michael Drosner, Konstantin Feise, Tanja Fischer, Gerd Gauglitz, Peter Arne Gerber, Sonja Grunewald, Katharina Herberger, Anja Jung, Syrus Karsai, Gerd Kautz, Carsten Philipp, Daniela Schaedel, Anna-Theresa Seitz, Alexander Nast
Summary: This guideline aims to provide specific treatment recommendations for various skin lesions, ensuring safe and effective laser therapy. It emphasizes the importance of understanding the differences in treatment methods for different types of skin conditions to avoid unnecessary risks.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2022)
Article
Dermatology
Helena Isabell Riedemann, Morna F. Schmidt, Jens Malte Baron
Summary: A scar is the result of a deep tissue defect during the wound healing process. Disorders can occur at all stages, leading to impaired wound healing or the formation of atrophic and hypertrophic scars or keloids. This article focuses on treating pathologic scars, which may cause functional limitations, complaints, and stigmatization. Medical, physical, surgical, and laser therapeutic approaches are pursued, based on an understanding of pathophysiological mechanisms and a comprehensive therapeutic approach.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Article
Dermatology
Katharina Webollek, Yvonne Marquardt, Sylvia Wagner-Schiffler, Jens Malte Baron, Sebastian Huth
Summary: The purpose of this in vitro study was to investigate the molecular effects of dexpanthenol-containing ointment (DCO) after micro-needling. The results showed that DCO accelerated wound healing and did not impair the immunostimulatory properties of micro-needling. These findings can optimize the aftercare routine and reduce downtime for patients after microneedling procedures.
CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY
(2023)
Article
Allergy
Karsten Weller, Ana Maria Gimenez-Arnau, Jens Baron, Randolf Brehler, Marta Ferrer, Adriane Groffik, Sonja Grundmann, Thilo Jakob, Moises Labrador-Horrillo, Sabine Mueller, Petra Staubach, Gerda Wurpts, Martin Metz, Marcus Maurer
Summary: This study compared on-demand (OD) treatment versus daily maintenance treatment with non-sedating H1-antihistamines for chronic spontaneous urticaria. It also assessed the efficacy of rupatadine updosing and investigated potential long-term disease-modifying effects. The results showed that daily rupatadine treatment significantly improved disease activity and quality of life during treatment, but there was no significant difference after discontinuation of rupatadine.
Article
Dermatology
Helena Isabell Riedemann, Morna F. Schmidt, Jens Malte Baron
Summary: A scar is formed as part of the wound healing process, involving inflammation and the proliferation of connective tissue cells. Pathological scars can result in impaired wound healing or the formation of atrophic and hypertrophic scars or keloids. This article focuses on the treatment of pathological scars using medical, physical, surgical, and laser therapeutic approaches, based on an understanding of the underlying mechanisms and interdisciplinary therapeutic approach.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Article
Multidisciplinary Sciences
Sebastian Huth, Laura Huth, Ruth Heise, Yvonne Marquardt, Linda Lopopolo, Marta Piecychna, Peter Boor, Guenter Fingerle-Rowson, Aphrodite Kapurniotu, Amir S. Yazdi, Richard Bucala, Juergen Bernhagen, Jens Malte Baron
Summary: The study found that MIF and D-DT play important roles in the development and progression of UVB-induced non-melanoma skin cancer (NMSC), but have no major effects on chemically-induced skin tumors. These findings suggest that MIF and D-DT are potential therapeutic targets for the prevention of photocarcinogenesis but not chemical carcinogenesis.
SCIENTIFIC REPORTS
(2023)
Article
Allergy
Conny Hoeflich, Galina Balakirski, Zuzanna Hajdu, Jens Malte Baron, Katharina Fietkau, Hans F. Merk, Ulrich Strassen, Henning Bier, Wolfgang Dott, Hans-Guido Muecke, Wolfgang Straff, Gerda Wurpts, Amir S. Yazdi, Adam Chaker, Stefani T. M. Roeseler
Meeting Abstract
Dermatology
Helena Isabell Riedemann, Yvonne Marquardt, Manuela Jansen, Jens Malte Baron, Sebastian Huth
LASERS IN SURGERY AND MEDICINE
(2023)
Meeting Abstract
Cell & Tissue Engineering
Rahul Rimal, Yvonne Marquardt, Suzana Djeljaidni, Thomas Neovalis, Dmitry Chigrin, Matthias Wessling, Jens Malte Baron, Martin Moeller, Smriti Singh
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Dermatology
L. Huth, Y. Marquardt, S. Huth, L. Schmitt, K. Prescher, P. Winterhalder, T. Steiner, F. Hoelzle, M. Eble, J. M. Baron
EXPERIMENTAL DERMATOLOGY
(2021)
Meeting Abstract
Dermatology
S. Huth, L. Huth, Y. Marquardt, M. Cheremkhina, R. Heise, J. M. Baron
EXPERIMENTAL DERMATOLOGY
(2021)
