Review
Biochemistry & Molecular Biology
Priti Gupta, Shilpa Sharma, Shagufta Jabin, Sapana Jadoun
Summary: Regenerative medicine and tissue engineering have great potential in addressing the limitations of traditional medical approaches. Chitosan, as a naturally derived biocompatible biomaterial, has played a significant role in tissue regeneration. It has unique properties and can be applied in various fields, such as agriculture, nutraceuticals, biomedicine, and food. Chitosan is also an important material for developing next-generation hydrogels and bio-scaffolds. Furthermore, chitosan can be easily modified to enhance its mechanical strength, biodegradability, and controlled release of bioactive molecules. Blending chitosan with other polymers or incorporating nanoparticles expands its possibilities in tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Engineering, Biomedical
Qi Feng, Dingguo Li, Qingtao Li, Xiaodong Cao, Hua Dong
Summary: This mini-review summarizes the fabrication strategies, unique characteristics, and applications of microgel assembly in tissue engineering and regenerative medicine, with a focus on 3D printing. The challenges for further applications of microgel assembly are also discussed.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Nikita Voloshin, Pyotr Tyurin-Kuzmin, Maxim Karagyaur, Zhanna Akopyan, Konstantin Kulebyakin
Summary: Immortalized cells are increasingly used in practical medicine due to their advantages, such as the ability to produce larger amounts of cells and use them for longer periods of time. However, they also have drawbacks, like the possibility of malignant transformation or major phenotype change. This review discusses ways to overcome these barriers and highlights specific fields of application where these concerns are minimal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Izeia Lukin, Itsasne Erezuma, Lidia Maeso, Jon Zarate, Martin Federico Desimone, Taleb H. Al-Tel, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Tissue engineering has become a viable medical alternative in response to the increasing lifespan of society. This review focuses on gelatin, a widely used biomaterial in tissue engineering, and discusses ongoing concerns and the latest developments in this field. Advancements in technology, such as 3D bioprinting and gelatin functionalization, have allowed for better replication of native tissue in gelatin-based systems. This breakthrough has implications for both the manufacturing process, making it more practical and cost-effective, and the future use of gelatin in disease detection and diagnosis rather than treatment.
Article
Chemistry, Physical
Ramon Rial, Natalia Hassan, Zhen Liu, Juan M. Ruso
Summary: The study successfully produced hydrogel microparticles with different morphologies and compositions by combining experimental and computational methods, incorporating drug-doped Hydroxyapatite in their inner matrix. The use of a microfluidic system to obtain crosslinked HMPs with homogeneous sizes and morphologies, as well as the adsorption kinetics and desorption profiles of the chosen drug models, were key aspects of the research. The development of these hydrogel microparticles with improved functionalities is crucial for innovative and sustainable solutions in regenerative medicine.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Review
Engineering, Biomedical
Michelle Koerselman, Lisanne C. M. Morshuis, Marcel Karperien
Summary: This article discusses the application of small molecules such as peptides, aptamers, and variable antigen-binding domain of heavy chain only antibodies in tissue engineering and regenerative medicine. It compares these biological functionalities with conventional antibodies and provides an overview of recent work in this area. The article focuses on the structural differences, modifications, and conjugation strategies of these biologicals, as well as the clinical trials involving them. The challenges associated with each of these biologicals are also highlighted.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Daniel Salthouse, Katarina Novakovic, Catharien M. U. Hilkens, Ana Marina Ferreira
Summary: This review highlights the importance of the immune response to biomaterials and discusses challenges and opportunities in the development and standardized assessment of the immune response to biomaterials, including the importance of endotoxin levels. It also explores the properties of biomaterials that impact the host immune response and the use of immunomodulatory biomaterials in regenerative medicine. Finally, a standardized in vitro pathway for evaluating the immune response to biomaterials (hydrogels) and their regenerative potential is proposed to ensure safety, consistency, and reduce costs and animal use.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Yiting Lou, Huiming Wang, Guanchen Ye, Yongzheng Li, Chao Liu, Mengfei Yu, Binbin Ying
Summary: This review discusses the important role of periosteum in bone defect repair and regeneration, as well as the need for developing tissue-engineered periosteum (TEP). The construction strategies for TEP are explored from the perspectives of materials, mechanical properties, cell-based strategies, and combination with growth factors. Current challenges and future perspectives for TEP development are also discussed.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Cell & Tissue Engineering
Umber Cheema
Summary: Tissue engineering is a multidisciplinary approach to building 3D human tissues in the laboratory. Despite three decades of advancements in medical sciences, there is limited use of tissue-engineered organs in humans. This position paper outlines the challenges and advancements in engineering specific tissues and organs, as well as the most successful technologies in tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biophysics
M. R. Poorna, R. Jayakumar, Jyh-Ping Chen, Ullas Mony
Summary: Induced pluripotent stem cells (iPSCs) have great potential in regenerative medicine, and improving culture methods, such as using hydrogels as substrates, can support iPSC adhesion, proliferation, and differentiation. Using hydrogels may help generate large numbers of clinical-grade cells for potential clinical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biophysics
Zhiwei Jiang, Jin He, Xueting Wang, Danji Zhu, Na Li, Lingfei Ren, Guoli Yang
Summary: Nanomaterial-based cell sheet technology has significant potential in regenerative medicine and tissue engineering. Different types of nanomaterials have been used to harvest cell sheets, categorized by mechanisms such as light-induced, thermo-responsive, magnetic-controlled, and ROS-induced. Studies have demonstrated successful outcomes in the regeneration of bone, skeletal muscle, cardiac tissue, tendon, angiogenesis, and osseointegration. However, challenges remain in terms of comprehensive preparation, safety, and cell quality.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Sunena Dhania, Manju Bernela, Ruma Rani, Minakshi Parsad, Sapna Grewal, Santosh Kumari, Rajesh Thakur
Summary: Tissue engineering technology utilizes biopolymer materials like polyhydroxyalkanoates to mimic and replace damaged tissues, with excellent biocompatibility and biodegradability. This technology has vast potential applications in areas such as bone grafts, cardiovascular repair, and nerve regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Chemistry, Multidisciplinary
Zhen Wang, Wasim Kapadia, Cuidi Li, Feng Lin, Ruben F. Pereira, Pedro L. Granja, Bruno Sarmento, Wenguo Cui
Summary: The human body is composed of four basic tissue types: epithelial, connective, muscular, and nervous tissues, each consisting of cells with similar functions. Three-dimensional bioprinting, based on histological categories, is a versatile technology that can form functional organ structures simply. This technology shows remarkable regenerative power and novel breakthroughs in specific tissues like muscle and nerve tissues.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Endocrinology & Metabolism
Maria Heim, Ian J. Nixon, Elaine Emmerson, Anthony Callanan
Summary: Primary hypothyroidism significantly impacts patient's quality of life. Current standard treatment has limitations, and combination therapy is an alternative. Tissue engineering and regenerative medicine techniques have potential to restore thyroid function, but more research and clinical translation are needed.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Alperen Abaci, Gulden Camci-Unal, Murat Guvendiren
Summary: Three-dimensional (3D) bioprinting is an emerging technology that can fabricate functional tissues and organs, replicating native tissue function. With its precise positioning of cellular materials and utilization of medical images, 3D bioprinting has enormous potential in biomedical applications, such as tissue engineering and regenerative medicine. It is a rapidly progressing field that has shown clinically relevant uses.
Article
Surgery
Karina M. da Costa, Amulya K. Saxena
Summary: This study reviewed the outcomes of laparoscopic surgery for neonate/infant malrotation and found it to be feasible and safe in hemodynamically stable patients, with a notable conversion rate and reintervention rate. The presence of volvulus was associated with symptom recurrence and the need for conversion during surgery. The commonly practiced approach involved laparoscopic Ladd's procedure with appendectomy and without cecopexy, which was associated with minor complications.
Article
Gastroenterology & Hepatology
Amulya K. Saxena, Ede Biro, Gerhard Sommer, Gerhard A. Holzapfel
Summary: This study investigated light microscopic changes after uniaxial stretching of the ovine esophagus and found that esophageal stretching > 1.3 leads to tears in muscle fibers and rupture of capillaries. These findings may explain the decrease in microcirculation and scarring during repairs of esophageal atresias under excessive tension, providing insights into impaired motility in the esophagus.
Review
Surgery
Karina Miura da Costa, Amulya Kumar Saxena
Summary: Laparoscopic cholecystectomy in children is safe, although complications such as bile duct injury are a concern. Most complications are minor, but nearly 1/5 of cases require interventions, mainly due to bleeding and biliary tree complications.
UPDATES IN SURGERY
(2021)
Letter
Surgery
Joshua J. Cave, Amulya K. Saxena
SURGICAL LAPAROSCOPY ENDOSCOPY & PERCUTANEOUS TECHNIQUES
(2021)
Review
Surgery
Ana S. Marinho, Amulya K. Saxena
Summary: This systematic review analyzed the outcomes of thoracoscopic repair of esophageal atresia (EA) between primary and staged repairs. Results showed that there were no significant differences in outcomes such as re-fistulation, anastomotic leaks, conversion rates, and mortality between primary and staged repairs. Successful repair with low complication rates was reported for both approaches.
SURGICAL LAPAROSCOPY ENDOSCOPY & PERCUTANEOUS TECHNIQUES
(2021)
Review
Surgery
Karina Miura da Costa, Amulya Kumar Saxena
Summary: This study reviewed outcomes in pediatric ACS/DAC after CDH repair, finding that ACS/DAC occur more frequently in neonates and a clear rationale behind the decision to perform DAC is lacking, with silo being the preferred approach. Criteria need to be established for DAC in CDH cases with large herniated content and small abdominal volume to prevent ACS.
UPDATES IN SURGERY
(2021)
Review
Surgery
Gratiana O. Alqadi, Amulya K. Saxena
Summary: This study reviewed the literature on the psychological aspects of patients with pectus excavatum (PE) and pectus carinatum (PC). The majority of studies reported an improvement in overall quality of life after surgery, including social and physical function, body image, and self-esteem. However, there is no consensus on the correlation between the severity of chest wall deformities and psychological issues.
Article
Biochemistry & Molecular Biology
Magdalena Grill, Isaac Lazzeri, Andrijana Kirsch, Nina Steurer, Tanja Grossmann, Michael Karbiener, Ellen Heitzer, Markus Gugatschka
Summary: Reinke's edema is a voice disorder associated with smoking and voice abuse, characterized by changes in vocal fold fibroblasts. Differential expression analysis between RE and control VFF revealed upregulated genes and pathways involved in extracellular matrix remodeling, inflammation, and fibrosis, while downregulated genes and pathways were associated with ECM degradation, cell cycle control, and proliferation.
Review
Pediatrics
Manish Pathak, Amulya K. Saxena
Summary: This study reviewed the literature on laparoscopic management of cloaca. The results showed that laparoscopic surgery could be used for repairing high rectum and performing urogenital separation, but there is currently no conclusive evidence to support that laparoscopic repair has better functional outcomes than open surgery.
JOURNAL OF PEDIATRIC UROLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Nida Mushtaq, Elliot Elwood, Esther Westwood, Alexander Macdonald, Amulya K. Saxena, Josephine Bretherton
Summary: Acute intestinal obstruction is a common emergency in pediatric surgery and can result from various causes, including congenital anomalies. Intestinal malrotation and Meckel's diverticulum are two common congenital anomalies that can coexist.
Article
Multidisciplinary Sciences
Ede Biro, Gerhard Sommer, Gerd Leitinger, Hajnalka Abraham, Daniel J. Kardos, Zsolt Oberritter, Amulya K. Saxena
Summary: Esophageal biomechanical studies were conducted to investigate structural changes and obtain biomechanical values for tissue-engineered esophagus. Uniaxial stretching tests were performed on 1-month-old lamb esophagus samples. Results showed that stretching over 30% led to significant alterations in the intra- and extracellular structure, indicating the disruption of bonds. These findings are important for understanding structural changes in repaired esophageal atresias and for tissue engineering of the esophagus.
SCIENTIFIC REPORTS
(2023)
Review
Pediatrics
Eva Sorensen, Connie Yu, Shu-Ling Chuang, Paola Midrio, Leopoldo Martinez, Mathew Nash, Ingo Jester, Amulya K. K. Saxena
Summary: The aim of this multicenter retrospective study and literature review was to review management and outcomes of neonatal esophageal perforation (NEP). The study found that NEP during NGT insertion is rare even in premature infants. In this small cohort, conservative management seems to be safe. A larger sample size will be necessary to answer questions on efficacy of antibiotics, antacids and NGT re-insertion time frame in NEP.
Article
Pediatrics
Amulya Saxena, Annika Mutanen, Ramon Gorter, Andrea Conforti, Pietro Bagolan, Paolo De Coppi, Tutku Soyer
Summary: This study reviewed the literature on the management of neonatal ovarian simple cysts and formulated a consensus statement based on current evidence and peer practice. Although there is limited data on the potential benefits and complications of prenatal ultrasound-guided aspiration, neonates after such procedures should be followed for 6 months. Neonates with simple ovarian cysts larger than 4 cm should undergo surgical interventions within the first two weeks of life, with laparoscopic cyst aspiration and fenestration using bipolar instruments as the preferred approach. Ultrasound follow-up should be done at 3 months after surgical intervention and every 3 to 4 months with the conservative approach until 1 year.
EUROPEAN JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Pediatrics
Ophelia Aubert, Hind Zaidan, Hanna Garnier, Amulya K. Saxena, Salvatore Cascio
Summary: Most recommendations of the EAU/ESPU guidelines are being followed by EUPSA members; however, personal and institutional practice impact decision making. Hormonal therapy in bilateral UDT, management of vanishing testes, and UDT in postpubertal boys could be improved.
EUROPEAN JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Pediatrics
Maja Raicevic, Amulya K. Saxena
Summary: The quality and benefits of laparoscopic pyloromyotomy videos on YouTube are limited, with a small number of videos and lack of content. English videos are predominant and have higher views.
MINERVA PEDIATRICS
(2022)