Journal
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
Volume 88-89, Issue -, Pages 48-85Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pnmrs.2015.05.001
Keywords
Oriented-sample solid-state NMR; Orientational restraints; Membrane proteins; Antimicrobial peptides; Macroscopic orientation
Funding
- Danish National Research Foundation [DNRF 0059]
- Carlsbergfondet
- Novo Nordisk Foundation
- Danish Natural Science Research Council
- Lundbeckfonden
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Oriented-sample solid-state NMR represents one of few experimental methods capable of characterising the membrane-bound conformation of proteins in the cell membrane. Since the technique was developed 25 years ago, the technique has been applied to study the structure of helix bundle membrane proteins and antimicrobial peptides, characterise protein-lipid interactions, and derive information on dynamics of the membrane anchoring of membrane proteins. We will review the major developments in various aspects of oriented-sample solid-state NMR, including sample-preparation methods, pulse sequences, theory required to interpret the experiments, perspectives for and guidelines to new experiments, and a number of representative applications. (C) 2015 Elsevier B.V. All rights reserved.
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