Article
Microbiology
Francesca Oberto, Emanuele Carella, Claudio Caruso, Pier Luigi Acutis, Davide Lelli, Luigi Bertolotti, Loretta Masoero, Simone Peletto
Summary: The aim of this study was to develop a qualitative PCR assay that allows the discrimination of BuHV-1, BoHV-1 and BoHV-5. Specific nucleotide sequences of BuHV-1, BoHV-1 and BoHV-5 were identified through the alignment of homologous genes. The design of the primers and the optimization of the PCR assay were focused on the target sequences located on the portions of gD, gE and gG genes. This qualitative PCR assay allowed the differentiation of BoHV-1 and BoHV-5 infections in both cattle and water buffaloes, as well as heterologous BuHV-1 infections in bovine.
Review
Virology
Ifeanyi Kingsley Uche, Konstantin G. Kousoulas, Paul J. F. Rider
Summary: The development of cancer disrupts anti-tumor immunity needed for surveillance and elimination of tumor cells, with immunotherapy aiming to restore or establish these responses. However, a significant proportion of patients do not respond equally to immunotherapies, partially due to the immunosuppressive tumor microenvironment. Current research focuses on mechanisms of immunosuppression in the TME, with the goal of promoting anti-tumor immune responses.
Article
Biochemistry & Molecular Biology
Wencai Qiu, Xiuyan Ding, Shitao Li, Yongming He, Liqian Zhu
Summary: The study demonstrates that BoHV-1 has oncolytic effects against human lung adenocarcinoma in vivo. BoHV-1 infection reduced HDAC protein levels in A549 cells and showed synergy with the HDAC inhibitor TSA. In the mouse xenograft model, BoHV-1 effectively infected tumors and suppressed tumor growth, potentially through interaction with HDACs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Chang Liu, Weifeng Yuan, Hao Yang, Junqing Ni, Linke Tang, Heci Zhao, Donna Neumann, Xiuyan Ding, Liqian Zhu
Summary: Phospholipase C gamma 1 (PLC-gamma 1) plays an important role in the replication process of bovine herpesvirus 1 (BoHV-1), specifically in the Golgi apparatus.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yang Liu, Qi Zhang, Min Zou, Jin Cui, Xiaojie Shi, Linjie Li, Faxing Wu, Xingang Xu
Summary: This study investigated the mechanism of Bovine herpesvirus-1 (BoHV-1) entry into MDBK cells. It was found that BoHV-1 entry is dependent on the acidic environment of endosomes and requires the dynamin protein. Moreover, clathrin-mediated endocytosis and caveolin-mediated endocytosis play a role in promoting BoHV-1 entry.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Chang Liu, Xiuyan Ding, Long Chang, Weifeng Yuan, Shitao Li, Liqian Zhu
Summary: This study found that PLC-gamma 1 interacts with beta-catenin, and the PLC-gamma 1 signaling pathway potentially regulates beta-catenin signaling stimulated by BoHV-1 infection by affecting the subcellular localization of p-beta-catenin(S552).
VETERINARY MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wenxiao Liu, Jiabing Hong, Jinglong Duan, Bo Jiang, Runan Zhu, Jing Cheng, Ping Wang, Yongqing Li
Summary: In this study, a blocking ELISA was developed to detect neutralizing antibodies against bovine herpesvirus 1 (BoHV-1) using a neutralizing monoclonal antibody (3F8) and protein gD. The assay showed high sensitivity and specificity and a significant correlation with the virus neutralization test. A serological survey in dairy farms in Beijing revealed a high risk of BoHV-1 infection from 2012 to 2017, but protection after implementing an immunization program in 2018.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Chang Liu, Weifeng Yuan, Hao Yang, Junqing Ni, Linke Tang, Heci Zhao, Donna Neumann, Xiuyan Ding, Liqian Zhu
Summary: During productive infection of bovine herpesvirus 1 (BoHV-1), activated form of phospholipase C gamma 1 (PLC-gamma 1), p-PLC-gamma 1(S1248), is partially located in the Golgi apparatus and associated with the viral protein gD. This phosphorylated PLC-gamma 1(S1248) may function as an escort during the trafficking of progeny virions from the Golgi apparatus to the plasma membrane.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Aleksandra Synowiec, Agnieszka Dabrowska, Magdalena Pachota, Meriem Baouche, Katarzyna Owczarek, Wojciech Nizanski, Krzysztof Pyrc
Summary: Feline herpesvirus 1 (FHV-1) is a common virus in cats, causing respiratory diseases and ocular lesions. The available vaccine is not able to prevent infection or limit virus shedding. This study examined the entry mechanisms of FHV-1 into host cells and found that it enters via endocytosis.
JOURNAL OF VIROLOGY
(2023)
Article
Veterinary Sciences
Kassahun Asmare, Kassaye Aragaw, Berhanu Sibhat, Takele Abayneh, Esayas Gelaye, Belayneh Getachew, Sharon Egan
Summary: This study aimed to estimate the seroprevalence of bovine herpesvirus 1 (BHV-1) and identify potential risk factors in dairy cattle in central and southern Ethiopia. The study showed that BHV-1 had an animal-level seroprevalence of 30.0% and a herd-level seroprevalence of 75.5%. The logistic regression model revealed that adult animals (> 2.5 years) had a higher seroprevalence of BHV-1, and arranging animals in a tail-to-tail fashion appeared to be protective against BHV-1 infection.
PREVENTIVE VETERINARY MEDICINE
(2023)
Article
Virology
Fouad S. El-Mayet, Gabriela Toomer, Jeffery B. Ostler, Kelly S. Harrison, Vanessa Claire Santos, Nishani Wijesekera, Erin Stayton, Jerry Ritchey, Clinton Jones
Summary: Steroid hormone fluctuations are predicted to enhance or initiate bovine herpesvirus 1 (BoHV-1) replication and virus spread in cattle. Progesterone stimulates BoHV-1 productive infection and sporadically induces reactivation from latency, while heat stress augments this reactivation.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Kevin Desaulniers, Levine Ortiz, Caroline Dufour, Alix Claudel, Melodie B. Plourde, Natacha Merindol, Lionel Berthoux
Summary: Introducing two mutations into the endogenous human TRIM5 gene using CRISPR-Cas9-mediated HDR significantly enhances TRIM5 alpha's ability to restrict HIV-1. Biallelic modification is crucial for achieving significant levels of inhibition by TRIM5 alpha.
Article
Virology
Iris Maria Forte, Paola Indovina, Serena Montagnaro, Aurora Costa, Carmelina Antonella Iannuzzi, Francesca Capone, Rosa Camerlingo, Anna Maria Malfitano, Francesca Pentimalli, Gianmarco Ferrara, Massimiliamo Quintiliani, Giuseppe Portella, Antonio Giordano, Roberto Ciarcia
Summary: Research has shown that caprine herpesvirus 1 (CpHV-1) has a cytotoxic effect on malignant mesothelioma (MM) cells and synergizes strongly with cisplatin, without affecting normal mesothelial cells. Further studies are needed to understand the mechanisms underlying CpHV-1's selective action on MM cells, but the data suggest that the CpHV-1-cisplatin combination could be a feasible strategy against MM.
Article
Virology
Soumya Sucharita, Suresh Tikoo, Sylvia Van Drunen Littel-van den Hurk
Summary: VP8, the most abundant tegument protein of bovine herpesvirus-1, interacts with glycoprotein M (gM) to localize to the Golgi apparatus and be packaged into mature virions.
Article
Virology
Beth H. Thompson, Colin P. Sharp, Inga R. Dry, Robert G. Dalziel, Eleanor R. Gaunt
Summary: This study validates the pro-viral roles of cellular protein TTC4 and heat shock protein 90 (HSP90) during BoHV-1 replication. TTC4 expression is upregulated during infection, depletion impairs protein production, and overexpression increases infectious virus production. Inhibition of HSP90 using geldanamycin inhibits protein production and viral genome replication.