Article
Immunology
Grant C. Weaver, Richa Arya, Christine L. Schneider, Amy W. Hudson, Lawrence J. Stern
Summary: This article reviews the structural biology of viral immunoevasins, introduces the current status of computational structure prediction algorithms, and predicts and evaluates the structural models for U20 and U21 proteins, providing insights into viral immune evasion.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Shivai Gupta, Danmeng Li, David A. Ostrov, Cuong Q. Nguyen
Summary: This study proposes a novel therapeutic approach for SjS by blocking MHC class II I-Ag-7 antigen presentation in NOD mice. The results demonstrate that treating NOD mice with TATD or 8-Azaguanine can alleviate SjS symptoms and reduce the severity of the disease.
Article
Biochemistry & Molecular Biology
Ankur Saikia, Andries Hadeler, Pranathi Prasad, Martin Zacharias, Sebastian Springer
Summary: This study investigates the importance of peptide exchange in immunodominance and uncovers the mechanism of peptide exchange under low pH conditions. The results demonstrate that low pH triggers peptide exchange and provide insights into the individual steps of the exchange reaction.
Article
Immunology
Karen Toledo-Stuardo, Carolina H. Ribeiro, Andrea Canals, Marcela Morales, Valentina Garate, Jose Rodriguez-Siza, Samantha Tello, Marco Bustamante, Ricardo Armisen, Douglas J. Matthies, Gerald Zapata-Torres, Patricio Gonzalez-Hormazabal, Maria Carmen Molina
Summary: This study revealed the association between MICA gene polymorphisms and susceptibility and prognosis of gastric cancer. Different MICA alleles were found to be significantly correlated with the risk and survival rates of gastric cancer patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Natsumi Mizuno, Yoshiki Yanagawa
Summary: In this study, it was found that tofacitinib enhanced IFN-gamma-induced MHC II expression in macrophages by transcriptional regulation through induction of CIITA, while decreasing the expression of CD86.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Oncology
Adrianna Piatakova, Ingrid Polakova, Jana Smahelova, Shweta Dilip Johari, Jaroslav Nunvar, Michal Smahel
Summary: Tumor-associated macrophages (TAMs) play a crucial role in the tumor microenvironment and their response to immunotherapy can vary depending on the expression of MHC-I molecules in the tumor. This study found that TAMs in tumors with distinct MHC-I expression levels show different immune reactions and cytotoxic capabilities. Consideration of these differences in TAMs is important for the development of effective cancer immunotherapies.
Article
Multidisciplinary Sciences
Katherine R. Martin, Katherine L. Mansfield, Anna E. Savage
Summary: Characterizing MHC genes' polymorphism is crucial for understanding vertebrates' immune response to disease. This study explores the immunogenetic variation in sea turtles afflicted by fibropapillomatosis, a global infectious tumour disease. Extensive variation and trans-species polymorphism were recovered in MHC class I genes among green and loggerhead sea turtles. Supertyping analysis identified three functional MHC supertypes corresponding to well-supported clades in the phylogeny. Positive selection at seven amino acid sites in the class I exon was observed. One allele weakly associated with a specific tumour texture was found, suggesting a potential link between MHC diversity and disease outcome. This study highlights the importance of evaluating genetic predictors of disease, including MHC and other functional markers, in sea turtles.
ROYAL SOCIETY OPEN SCIENCE
(2022)
Article
Biology
Orsolya Vincze, Claire Loiseau, Mathieu Giraudeau
Summary: High MHC class I diversity is associated with significantly lower richness of haemosporidian parasites in birds, indicating more efficient protection against intracellular pathogens. However, these associations were only detected when MHC diversity was assessed using cloning, suggesting the need for further clarification on the use of different genotyping methods for MHC loci quantification.
Article
Multidisciplinary Sciences
William H. Palmer, Marco Telford, Arcadi Navarro, Gabriel Santpere, Paul J. Norman
Summary: The diversity of herpesvirus proteins is related to the evasion of HLA presentation. Latent herpesvirus proteins have increased peptide sequence diversity and greater population structure in HLA binding, while lytic proteins are conserved. Recognition of EBV isolates by high-frequency HLA class I allotypes is less efficient, and CD8+ T cell epitopes inversely correlate with the frequency of HLA class I recognition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Patricia T. Illing, Andy van Hateren, Rachel Darley, Nathan P. Croft, Nicole A. Mifsud, Samuel King, Lyudmila Kostenko, Mandvi Bharadwaj, James McCluskey, Tim Elliott, Anthony W. Purcell
Summary: Abacavir hypersensitivity syndrome occurs in individuals with a specific gene expression, altering immune responses. Research shows that abacavir affects the assembly of HLA-B*57:01 complexes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Brett A. Eyford, Maciej J. Lazarczyk, Kyung Bok Choi, Merina Varghese, Hitesh Arora, Suresh Kari, Lonna Munro, Cheryl G. Pfeifer, Allison Sowa, Daniel R. Dickstein, Dara L. Dickstein, Wilfred A. Jefferies
Summary: This study investigates the significance of the cytoplasmic tail of MHC-I in synaptic signaling and its impact on the expression of synaptic glutamate receptors. The findings reveal that the Y321F substitution influences the expression of AMPAR subunits and alters the phosphorylation of key kinases. These results illuminate the crucial role of MHC-I in AMPAR function and synaptic plasticity modulation.
SCIENTIFIC REPORTS
(2023)
Review
Immunology
Andy van Hateren, Tim Elliott
Summary: The selection of peptides presented by MHC-I molecules plays a crucial role in immune responses. The proteins tapasin and TAPBPR coordinate the peptide selection process, ensuring that MHC-I molecules acquire high-affinity-binding peptides. New structural analyses have provided insights into the mechanisms of tapasin's role within the peptide-loading complex and TAPBPR's ability to independently perform peptide editing. These structures reveal the intricate interactions between tapasin, TAPBPR, and MHC-I, as well as the complementary roles of calreticulin and ERp57 in achieving peptide editing.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Marion Rambault, Florence B. Gilbert, Philippe Roussel, Alexia Tessier, Pierre Germon, Nathalie Winter, Aude Remot
Summary: Bovine mastitis, caused by bacterial infection, not only leads to economic losses and health problems in animals, but also affects their welfare. Neutrophils play a crucial role in eliminating pathogens and are responsible for the increase in somatic cell count during mastitis. Recent research reveals that a subset of neutrophils expressing major histocompatibility complex class II have regulatory capacities in cattle, providing new insights into the immune response during mastitis.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Computer Science, Artificial Intelligence
Benjamin Alexander Albert, Yunxiao Yang, Xiaoshan M. M. Shao, Dipika Singh, Kellie N. N. Smith, Valsamo Anagnostou, Rachel Karchin
Summary: Researchers propose a method based on long short-term memory ensembles and transfer learning to predict effective neoepitopes that elicit an immune response. This method can help address the challenge of predicting immunogenicity of neoepitopes in developing personalized cancer vaccines. Compared to other state-of-the-art classifiers, this method significantly improves the prediction of epitope presentation.
NATURE MACHINE INTELLIGENCE
(2023)
Article
Biochemistry & Molecular Biology
Gemma Palomar, Katarzyna Dudek, Magdalena Migalska, J. W. Arntzen, G. Francesco Ficetola, Dusan Jelic, Elizabeth Jockusch, Inigo Martinez-Solano, Masatoshi Matsunami, H. Bradley Shaffer, Judit Voros, Bruce Waldman, Ben Wielstra, Wieslaw Babik
Summary: The study found a positive correlation between MHC-I diversity and the diversity of two APGs (TAP1 and TAP2) in salamanders, supporting their coevolution. However, no consistent effect was detected for the other three APGs (PSMB8, PSMB9, and TAPBP). This suggests that while coevolution occurs in salamanders, it does not limit the expansion of the MHC-I gene family.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Alexander E. Yarawsky, Stefanie L. Johns, Peter Schuck, Andrew B. Herr
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Chemistry, Physical
Luiza L. Knittel, Huaying Zhao, Ai Nguyen, Antonio Miranda, Peter Schuck, Alioscka A. Sousa
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)
Article
Multidisciplinary Sciences
Alexander E. Conicella, Rui Huang, Zev A. Ripstein, Ai Nguyen, Eric Wang, Thomas Lohr, Peter Schuck, Michele Vendruscolo, John L. Rubinstein, Lewis E. Kay
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Microbiology
Zhaochun Chen, Ronald E. Engle, Chen-Hsiang Shen, Huaying Zhao, Peter W. Schuck, Emily J. Danoff, Hanh Nguyen, Norihisa Nishimura, Kevin W. Bock, Ian N. Moore, Peter D. Kwong, Robert H. Purcell, Sugantha Govindarajan, Patrizia Farci
Article
Biophysics
Huaying Zhao, Ai Nguyen, Samuel C. To, Peter Schuck
Summary: Analytical ultracentrifugation (AUC) provides absolute measurements of macromolecular parameters, but many instruments suffer from significant systematic errors. External calibration can greatly improve measurement precision and consistency.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Biochemical Research Methods
Susan M. Clardy, David H. Lee, Peter Schuck
Summary: Advances in polymer science have expanded the applications of protein-polymer conjugates as biopharmaceuticals and biotechnology reagents. This study demonstrates the use of multisignal sedimentation velocity analytical ultracentrifugation to measure the polymer-to-protein ratio in antibody-drug conjugates of varying ratios and heterogeneity levels. The results show that multisignal sedimentation velocity can accurately provide crucial information on attributes like polymer-to-protein ratio.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Robert W. Harkness, Yuki Toyama, Zev A. Ripstein, Huaying Zhao, Alexander I. M. Sever, Qing Luan, Jacob P. Brady, Patricia L. Clark, Peter Schuck, Lewis E. Kay
Summary: DegP is an oligomeric protein with dual protease and chaperone activity that regulates protein homeostasis and virulence factor trafficking in gram-negative bacteria. Different oligomeric states are adopted by DegP to facilitate its function, with resting hexamers formed when not engaged to substrates and cage or bowl-like structures formed when bound to substrates, leading to increased proteolytic activity. The self-assembly mechanism of apo-DegP involves an ensemble of oligomeric states mediated by self-assembly of trimers, which can shift towards larger oligomeric assemblies under physiological conditions to respond to biological insults.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Erik W. Martin, Tyler S. Harmon, Jesse B. Hopkins, Srinivas Chakravarthy, J. Jeremias Incicco, Peter Schuck, Andrea Soranno, Tanja Mittag
Summary: This study investigates the assembly kinetics of a prion-like domain using liquid-liquid phase separation and identifies two kinetic regimes on different timescales characterized by the size distribution of clusters. The research uncovers the nucleation mechanisms of biological protein phase separation and reveals multiple kinetic regimes on the micro- to millisecond timescale.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Antonio Rodriguez-Blazquez, Arturo Carabias, Alba Moran-Vaquero, Sergio de Cima, Juan R. Luque-Ortega, Carlos Alfonso, Peter Schuck, Jose Antonio Manso, Sandra Macedo-Ribeiro, Carmen Guerrero, Jose M. de Pereda
Summary: C3G activation is regulated by phosphorylation and interaction with Crk adaptor proteins, revealing a two-factor mechanism for tight control of its activity.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Huaying Zhao, Di Wu, Sergio A. Hassan, Ai Nguyen, Jiji Chen, Grzegorz Piszczek, Peter Schuck
Summary: The N-protein of SARS-CoV-2 plays a crucial role in viral assembly and RNA scaffolding. It promotes LLPS, forming dense droplets that aid in the assembly of ribonucleoprotein particles. Through experiments, simulations, and mutational analysis, researchers have discovered an unknown oligomerization site that contributes to LLPS and is necessary for the assembly of protein-nucleic acid complexes. This finding presents a potential target for antiviral therapeutics.
Article
Microbiology
Peter Schuck, Huaying Zhao
Summary: Molecular mimicry of short linear interaction motifs is a key mechanism for viral proteins to bind host domains and hijack host cell processes. Through analyzing the sequence diversity of SARS-CoV-2, this study reveals that motif mimicry is a highly dynamic process that can broadly explore host motifs, allowing the virus to rapidly evolve the virus-host interface.
Article
Multidisciplinary Sciences
Huaying Zhao, Ai Nguyen, Di Wu, Yan Li, Sergio A. Hassan, Jiji Chen, Hari Shroff, Grzegorz Piszczek, Peter Schuck
Summary: Global SARS-CoV-2 sequencing efforts have identified mutations in the spike protein and other viral proteins, with the mutational landscape of the nucleocapsid protein exhibiting more variability than the spike protein. The N-G215C mutation in the Delta variant may stabilize protein-protein interaction interfaces and enhance viral assembly efficiency, leading to greater infectivity. Through biophysical experiments, it was found that the N-G215C mutation shifts the unliganded protein from a dimeric to a tetrameric state, suggesting high plasticity in the N-protein's biophysical properties.
Review
Chemistry, Multidisciplinary
Alioscka A. Sousa, Peter Schuck, Sergio A. Hassan
Summary: The use of nanoparticles in biomedicine has transitioned from concept to clinical trials, with ultrasmall nanoparticles showing potential applications in diagnosis and therapy. The ability to control the behavior of these nanoparticles is limited, and further advances are needed in nanoengineering and chemical synthesis to fully understand their interactions in biological environments.
NANOSCALE ADVANCES
(2021)
Article
Medicine, Research & Experimental
Sumit K. Chaturvedi, Arun Parupudi, Kristian Juul-Madsen, Ai Nguyen, Thomas Vorup-Jensen, Sonia Dragulin-Otto, Huaying Zhao, Reza Esfandiary, Peter Schuck
Article
Chemistry, Multidisciplinary
Andre L. Lira, Rodrigo S. Ferreira, Ricardo J. S. Torquato, Maria Luiza Oliva, Peter Schuck, Alioscka A. Sousa
NANOSCALE ADVANCES
(2019)