Journal
JOURNAL OF VIROLOGY
Volume 87, Issue 14, Pages 8257-8260Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00163-13
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- Deutsche Forschungsgemeinschaft (DFG)
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We established a reverse genetics system for Nyamanini virus (NYMV) and recovered green fluorescent protein (GFP)-expressing virus from full-length cDNA. Using this technology, we assessed the functions of two poorly characterized viral genes. NYMV lacking open reading frame 2 (ORF2) could not be rescued, whereas virus lacking ORF4 was replication competent. ORF4-deficient NYMV readily established a persisting noncytolytic infection but failed to produce infectious viral particles, supporting the view that ORF4 represents an essential factor for NYMV particle assembly.
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