Article
Biochemistry & Molecular Biology
J. Bernard Heymann, Roberto Marabini, Mohsen Kazemi, Carlos Oscar S. Sorzano, Maya Holmdahl, Joshua H. Mendez, Scott M. Stagg, Slavica Jonic, Eugene Palovcak, Jean-Paul Armache, Jianhua Zhao, Yifan Cheng, Grigore Pintilie, Wah Chiu, Ardan Patwardhan, Jose-Maria Carazo
JOURNAL OF STRUCTURAL BIOLOGY
(2018)
Article
Cell Biology
J. Bernard Heymann, Camasamudra Vijayasarathy, Rick K. Huang, Altaira D. Dearborn, Paul A. Sieving, Alasdair C. Steven
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biochemical Research Methods
J. Bernard Heymann
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2019)
Meeting Abstract
Biophysics
Bernard Heymann, Christopher K. E. Bleck, Robert N. Fariss, Alexandr Smirnov, Dennis C. Winkler, Camasamudram Vijayasarathy, Rick Huang, Altaira D. Dearborn, Paul A. Sieving, Alasdair C. Steven
BIOPHYSICAL JOURNAL
(2019)
Article
Microbiology
Ambroise Desfosses, Hariprasad Venugopal, Tapan Joshi, Jan Felix, Matthew Jessop, Hyengseop Jeong, Jaekyung Hyun, J. Bernard Heymann, Mark R. H. Hurst, Irina Gutsche, Alok K. Mitra
NATURE MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
J. Bernard Heymann
Summary: Bsoft offers tools for processing tomographic tilt series and interpreting tomograms. The field has advanced significantly in the past two decades, requiring refinement of old algorithms and development of new ones. Direct detectors now allow more efficient data collection and progress towards automation.
Article
Virology
J. Bernard Heymann, Bing Wang, William W. Newcomb, Weimin Wu, Dennis C. Winkler, Naiqian Cheng, Erin R. Reilly, Ru-Ching Hsia, Julie A. Thomas, Alasdair C. Steven
Article
Multidisciplinary Sciences
Erney Ramirez-Aportela, David Maluenda, Yunior C. Fonseca, Pablo Conesa, Roberto Marabini, J. Bernard Heymann, Jose Maria Carazo, Carlos Oscar S. Sorzano
Summary: This article introduces a method for local analysis of map-to-model fit in cryoEM, using a comparison of two local resolution maps and introducing FSC-Q as a quantitative estimation of how much of the model is supported by the signal content of the map.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Michael H. C. Buch, William W. Newcomb, Dennis C. Winkler, Alasdair C. Steven, J. Bernard Heymann
Summary: The portal protein of Herpes simplex virus 1 plays a crucial role in packaging its genome and maturation process, forming a complex with scaffold proteins. As the capsid angularizes during maturation, the portal protein moves outward and strengthens its contacts with the capsid shell. Most capsids contain a single portal protein supporting the assembly of procapsids.
Article
Computer Science, Interdisciplinary Applications
J. Bernard Heymann
Summary: This study aims to assess and improve the quality of cryoEM reconstructions by calculating the spectral SNR (SSNR) and radiation damage. Through alignment and calculation of several test cases of movie frames, it was found that alignment compensated for global movement, the effect on SSNR was negligible, and the subset SSNR confirmed residual movement. The progressive SSNR reflected radiation damage and signal accumulation decrease. The results contribute to the assessment of micrograph quality and progression of radiation damage.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2022)
Review
Ophthalmology
J. Bernard Heymann, Camasamudram Vijayasarathy, Robert N. Fariss, Paul A. Sieving
Summary: Retinoschisin (RS1) is crucial for maintaining retinal integrity, but mutations in RS1 cause X-linked retinoschisis (XLRS) and vision loss. The formation of cysts and schisis in the retina disrupts synaptic transmission. Gene augmentation therapy using AAV vectors is being evaluated as a potential treatment for XLRS. Understanding the secretion and molecular function of RS1 is essential for improving therapeutic strategies.
PROGRESS IN RETINAL AND EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
J. Bernard Heymann
Summary: In cryoEM, it is essential to consider the Ewald sphere/focus gradient and its implications for high resolution and handedness information recovery. Different approaches have been proposed, but the correct one remains uncertain. The traditional projection approximation breaks down at high acceleration voltages, but correct reconstruction along the Ewald spheres can avoid its limitations and reach resolutions to the Nyquist frequency.
JOURNAL OF STRUCTURAL BIOLOGY-X
(2023)
Article
Biology
J. Bernard Heymann
Summary: Bsoft is a software package developed for processing electron micrographs. It has over 150 programs for manipulating and understanding images. The primary aim is to provide powerful capabilities for processing large numbers of images.
Article
Biology
J. Bernard Heymann
Article
Biology
Evan S. Krystofiak, J. Bernard Heymann, Bechara Kachar
COMMUNICATIONS BIOLOGY
(2019)